Correlation between type 2 diabetes and DNA methylation and mRNA expression of APN, TNF-α and MCP-1 in abdominal adipose tissues in Xinjiang Uygur population
Related Articles |
Objective To investigate the relationship between type 2 diabetes mellitus (T2DM) onset and mRNA expression and promoter methylation of adiponectin (APN) , tumor necrosis factor alpha(TNF-α), monocyte chemotactic cytokines protein 1 (MCP-1) gene in abdominal adipose tissues of Xinjiang Uygur population. Methods The abdominal adipose tissue of Uyghur subjects were collected and divided into normal control group (NC, n= 50), obesity group（Ob, n = 48）and T2DM group( n = 26).The status of DNA methylation was detected by denaturing high performance liquid chromatography and the mRNA level was detected by real time-PCR. Results Compared the methylation positive rate in the NC, Ob and T2DM group, the result showed that APN was in turn increased (P<0.05); TNF-α was in turn decreased (P<0.01);.The of APN in control group was significantly higher than that of obesity and T2DM groups, while the relative mRNA level of TNF-α and MCP-1 in control group was significantly higher than that of obesity and T2DM groups(P<0.05).The mRNA expression of APN was significantly negative correlated with BMI, DBP, FPG, TG, LDL(P<0.05); The mRNA expression of TNF-α was significantly positive correlated with BMI, FPG, TG (P<0.05); The mRNA expression of MCP-1 was significantly positive correlated with body weight, BMI and waist circumference(P<0.05). DNA methylation of APN was significantly negative correlated with the mRNA expression level of APN (P < 0.01), and was was significantly positive correlated with plasma level of FPG, TC, TG and LDL (P<0.05); DNA methylation of TNF-α was significantly negative correlated with the mRNA expression level of TNF-α, BMI, FPG, TC, TG and LDL. Conclusion the different expression of APN, TNF-α and MCP-1 gene in abdominal adipose tissue may be a reason for the obesity and T2DM in the Xinjiang Uyghur population; In addition, the glucose and lipid metabolic disorder may result in DNA methylation, and then affect the mRNA expression of APN and TNF-α.