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Table of Content

    05 December 2022, Volume 42 Issue 12
    Original Articles
    Ischemic preconditioning attenuates endothelial injury induced by femoral artery puncture in rabbits
    LIU Peng, LI Kai-yuan, YANG Yang, MAN Yi-long, DU Feng-li, LIU Miao
    2022, 42(12):  1817-1822.  doi:10.16352/j.issn.1001-6325.2022.12.1817
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    Objective To investigate whether ischemic preconditioning (IP) attenuates endothelial injury induced by arterial puncture. Methods Rabbits were divided into control group, ischemic preconditioning group (IP group), femoral artery puncture group (P group) and ischemic preconditioning+femoral artery puncture group (IP-P group). The expression of PI3K, AKT and eNOS in endothelial tissue was determined by immuno-histochemistry. p-AKT and p-eNOS were detected by Western blot. eNOS was detected by RT-qPCR. Plasma NO was detected by enzyme-linked immunosorbent assay(ELISA). Results The expression of PI3K, AKT and eNOS in the vascular endothelium of IP-P group was higher than that from P group and lower than that from IP group(P<0.05). The expression of p-AKT and p-eNOS in IP-P group was higher than that from P group(P<0.05). The expression of eNOS in IP-P group was higher than that from P group and lower than that from IP group(P<0.05). Plasma NO level in IP-P group was higher than that in P group and lower than that in IP group (P<0.05). Conclusions IP promotes eNOS phos-phorylation and NO synthesis in rabbit vascular endothelium, which may be one of the potential mechanisms by which IP reduces endothelial injury before arterial puncture.
    Etomidate reduces acute lung injury induced by LPS in mice
    JING Jian-chuang, ZHANG Zhi-jun
    2022, 42(12):  1823-1828.  doi:10.16352/j.issn.1001-6325.2022.12.1823
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    Objective To investigate the effect of etomidate on acute lung injury (ALI) induced by lipopolysaccharide (LPS) in mice. Methods The mice were randomly divided into control group, model group, etomidate groups of low (5.0 mg/kg), medium (10.0 mg/kg) and high dose (20.0 mg/kg) and positive control (Xuebijing injection, 100 μL) group with 15 mice in each. After 12 hours, the Buxco pulmonary function meter was used to detect the mouse respiratory function indicators-lung compliance, respiratory frequency, airway resistance; ELISA method was used to determine the contents of inflammatory factors-tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) in the serum of mice; the wet weight/dry weight (W/D) ratio of the mouse lungs was calculated; HE staining microscopy was used to observe the pathological changes of lung tissue in mice; Western blot was used to test the expression of TXNIP and NLRP3 proteins in mouse lung tissue. In addition, TXNIP over-expression vector (PCDNA3.1-TXNIP, 5 nmol/L) was added on the basis of 20.0 mg/kg etomidate and negative control group (pcDNA3.1) was set to explore the effect of etomidate regulation through TXNIP/NLRP3 signaling pathway on LPS-induced ALI. Results Compared with the control group, the lung tissue of the model group showed inflammatory cell infiltration and alveolar wall thickening, the lung compliance and respiratory frequency were significantly reduced (P<0.05). The lung tissue pathology score, airway resistance, serum content of TNF-α and IL-6, lung W/D ratio, lung tissue TXNIP, NLRP3 protein expression were all increased significantly(P<0.05). Compared with the model group, the inflammatory cell infiltration and alveolar wall thickening of the lung tissue of the mice in the etomidate low, medium and high dose groups were relieved to varying degrees and the lung compliance and respiratory frequency were gradually increased (P<0.05). The lung tissue pathology score, airway resistance, serum TNF-α and IL-6 contents, lung W/D ratio, lung tissue TXNIP, NLRP3 protein expression were gradually decreased (P<0.05). Compared with pcDNA3.1 group, pcDNA3.1-TXNIP group reversed protective effect of etomidate on mice. Conclusions Etomidate inhibits inflammatory response and improves pathological damage of lung tissue.
    Construction and functional verification of AP-2 gene family eukaryotic expression vector
    MI Yue, JIANG Qi-wei, SHEN Yan-jie, ZHANG De-yu, YE Qi-nong
    2022, 42(12):  1829-1834.  doi:10.16352/j.issn.1001-6325.2022.12.1829
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    Objective To construct the eukaryotic expression recombinant plasmids of activator protein 2(AP-2) family genes and to identify the effect of AP-2α, AP-2β and AP-2γ on proliferation and migration of HepG2 cell line. Methods Ap-2α,AP-2β and AP-2γ gene were amplified with human mammary cDNA library as template and ligated with pCDNA3.0-FLAG vector, respectively. The eukaryotic expression vector pCDNA3.0-FLAG-AP-2α/β/γ was constructed and transfected into HepG2 cells. CCK8 assay and clone formation assay were used to detect the effect of AP-2 family genes on HepG2 cell proliferation. Scratch assay was used to detect the effect of AP-2 family genes on HepG2 cell migration. Results The pCDNA3.0-FLAG-AP-2α/β/γ were successfully constructed and expressed in HepG2 cells.AP-2 family genes significantly inhibited the proliferation and migration of HepG2 cells(P<0.05). Conclusions Ap-2α, AP-2β and AP-2γ may inhibit the proliferation and migration of HepG2 cells.
    Application of the steroid profiling detecting by LC-MS/MS in the diagnosis of primary aldosteronism
    GAO Yin-jie, XIE Shao-wei, LIU Shi-ying, LU Yi, ZHANG Fang, QIU Ling, TONG An-li
    2022, 42(12):  1835-1840.  doi:10.16352/j.issn.1001-6325.2022.12.1835
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    Objective To explore the characteristics of steroid hormone spectrum in patients with primary aldoste- ronism (PA) and to evaluate the diagnostic efficacy of 18-oxocortisol and 18-hydroxycortisol. Methods Fifty-nine PA patients including 41 cases of aldosterone-producing adenoma (APA) and 18 cases of idiopathic hyperaldosteronism (IHA)and 42 essential hypertension (EH) patients as control group diagnosed in Department of Endocrinology of Peking Union Medical College Hospital from November 2020 to November 2021 were enrolled in this study. The basic information and clinical characteristics of patients were collected and 8 kinds of steroid hormones,including mineral corticoids (pregnenolone,progesterone, 11-deoxycorticosterone, corticosterone, 18-OH-corticoster-one) and aldosterone, and metabolites 18-oxocortisol as well as 18-hydroxycortisol were detected by liquid chromatography-tandem mass spectrometry(LC-MS/MS).The differences of steroid hormone level from different groups were compared and their diagnostic efficacy was evaluated. Results The average level of progesterone in APA group was higher than that in EH group (P<0.05). The average level of 11-deoxycorticosterone in APA and IHA group was higher than that in EH group (both P<0.001). The average level of 18-OH-corticosterone in APA group was higher than that in EH group (P<0.001). The average level of aldosterone in APA and IHA group were higher than that in EH group(P<0.01). The average level of 18-oxocortisol in APA group was higher than that in IHA group and higher than that in EH group (all P<0.05). The average level of 18-hydroxycortisol in APA group was higher than that in EH group (P<0.001). As for the diagnostic efficiency of 18-oxocortisol and 18-hydroxycortisol differentiating PA from EH, when the cut-off value of 18-oxocortisol was 0.06 ng/mL, the sensitivity reached 74.6% with specificity as 88.1%; when the cut-off value of 18-hydroxycortisol was 1.44 ng/mL, the sensitivity was 71.2% and the specificity was 69.0%. As for the diagnostic efficiency of 18-oxocortisol and 18-hydroxycortisol recognizing APA, when the cut-off value of 18-oxocortisol was 0.11 ng/mL, the sensitivity was 73.2% and the specificity was 95.2%; when the cut-off value of 18-hydroxycortisol was 1.77 ng/mL, the sensitivity was 68.3% and the specificity was 78.6%. Conclusions The level of various steroid hormone precursors and metabolites in PA patients is significantly higher than those in EH patients. The detection of steroid hormone profiling by LC-MS/MS plays an important role in the diagnosis and typing of PA. In particular, 18-oxocortisol and 18-hydroxycortisol show unique advantages in the recognition of APA.
    hsa-Let-7a-5p inhibits fibrosis of fibroblasts from localized scleroderma through targeted binding of TGF-βR1
    WANG Li-quan, HUANG Jiu-zuo, YU Nan-ze, MA Xu-da, LI Tian-hao, LONG Xiao
    2022, 42(12):  1841-1849.  doi:10.16352/j.issn.1001-6325.2022.12.1841
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    Objective To investigate the effect of hsa-Let-7a-5p on the fibrosis process of localized scleroderma fibroblasts (LSFs) and its correlation with transforming growth factor-β receptor (TGF-βR) and TGF-β/Smad signaling pathway. Methods LSFs were infected with lentiviral vectors carrying hsa-Let-7a-5p and shTGF-βR1 and blank lentiviral vectors. The proliferation and migration of LSFs in each group were detected by CCK-8 method and scratch test. Real-time quantitative PCR (RT-qPCR), immunofluorescence and Western blot were used to detect the transfection efficiency, and the mRNA and protein levels of collagen type Ⅰ & Ⅲ, α-SMA, TGF-βR1, TGF-β, p-Smad2/3 in LSFs in each group. Results After virus infection of LSFs, the expression of TGF-βR1 in the shTGF-βR1 group was significantly lower than that in the control groups (P<0.05); the cell proliferation rate and migration of shTGF-βR1 group were inhibited(P<0.05); The mRNA and protein expression of collagen type Ⅰ & Ⅲ, α-SMA, TGF-β, p-Smad2/3 were significantly lower than those of the control groups(P<0.05). Bioinformatics analysis showed that hsa-Let-7a-5p can be matched with the target region of TGF-βR1 3′-UTR, and the expression of hsa-Let-7a-5p in the lentivirus transfection group was significantly increased compared with the control groups(P<0.05), the expression of TGF-βR1 was significantly decreased(P<0.05). The cell proliferation rate and migration ability of the hsa-Let-7a-5p group were decreased; the mRNA and protein expression of collagen type Ⅰ & Ⅲ, α-SMA, TGF-β, p-Smad2/3 in the hsa-Let-7a-5p group were significantly lower than those of the control groups(P<0.05). Conclusions hsa-Let-7a-5p regulates TGF-β/Smad pathway by targeting at TGF-βR1,inhibits proliferation and migration of LSFs, decreases fibrotic markers in LSFs thereby inhibiting fibrosis in localized scleroderma.
    Intrahippocampal injection of irisin ameliorates depression-like behaviors in mice
    LI Jing, HUANG Bing-xuan, XU Qi
    2022, 42(12):  1850-1854.  doi:10.16352/j.issn.1001-6325.2022.12.1850
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    Objective To investigate the effects of irisin on the depression-like behaviors in depression mice. Methods C57BL/6 mice were randomly divided into model and healthy groups. Mice in the model group were trained by open-space forced swim (OSFS) model and then were sub-divided into “model-control” and “model-irisin”. Healthy wild mice were bred normally and sub-divided into “healthy-control” and “healthy-irisin”groups. All mice in test group and in control groups were injected with 77 pmol irisin or the solvent (PBS) into both hippocampi by brain stereotactic injection, respectively. Depression-like behaviors were examined by forced swim test (FST) and tail suspension test (TST). Results Compared with the healthy ones, the model mice showed a markedly prolonged immobility time in FST(P<0.01) and TST(P<0.05), which indicated that the successfully established model can simulate the despair-like behaviors of patients with depression. Compared with the “model-control”, irisin can remarkably reduce the immobility time of “model-irisin” mice in FST(P<0.001) and TST(P<0.05). Conclusions Irisin can significantly improve the depression-like behaviors of OSFS depression mice.
    Toll-like receptor 3 activation combined with INFα inhibits the expression of DISC1 in hippocampal neurons of mice
    YAO Jing-hong, LI Jia-huan, LIU Zi-jian
    2022, 42(12):  1855-1860.  doi:10.16352/j.issn.1001-6325.2022.12.1855
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    Objective To explore the effect of Toll-like receptor 3 (TLR3) activation on the expression of neuronal disrupted-in-schizophrenia 1 (DISC1). Methods C57BL/6J mouse hippocampal neurons were incubated with polyI:C(1 μg/mL) or/and mIFNα(100 IU/mL) for 24 hrs. DISC1 mRNA and protein were detected by quantitive RT-PCR, immuno-cytochemistry staining and Western blot. The expression of STAT1, phospho-STAT1, p38 MAPK, phospho-p38 MAPK, GSK-3β, and phospho-GSK-3β was also detected by Western blot. Results Comparing with control group, the mRNA and protein expression of DISC1 in polyI:C group and IFNα group didn't show any changes, but significantly decreased with the co-stimulation by polyI:C and IFNα (P<0.05). In addition, the phosphorylation of STAT1 was significantly increased in both IFNα group and co-stimulation group(P<0.05) and the phosphorylation of p38 MAPK was elevated in polyI:C group and co-stimulation group (P<0.05).The expression of GSK-3β and phospho-GSK-3β had no changes. Conclusions The combined stimulations of IFNα and TLR3 activation may inhibit the expression of DISC1 potentially through JAK/STAT1 and p38 MAPK signaling pathways.
    Correlation of expression of lncRNA ZFAS1 and hsa-miR-372-3p in lung cancer tissues with the malignant characteristics and prognosis of patients
    YANG Ye, DANG Rong-guang, ZHANG Jing, DONG Chao, SONG Xue-jing
    2022, 42(12):  1861-1866.  doi:10.16352/j.issn.1001-6325.2022.12.1861
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    Objective To study the correlation of the expression of long non-coding RNA zinc finger antisense 1 (lncRNA ZFAS1) and hsa-miR-372-3p in lung cancer tissues with the malignant characteristics and prognosis of patients. Methods Eighty-two lung cancer patients hospitalized in Shijiazhuang People's Hospital from March 2015 to October 2016 were selected. The expression of ZFAS1 and miR-372-3p in lung cancer tissues and in adjacent tissues, the potential correlation of the level of ZFAS1 and miR-372-3p with malignant characteristics of patients were analyzed; Pearson method was used to analyze the relationship between ZFAS1 and miR-372-3p in lung cancer tissues; Kaplan-Meier was used to analyze the correlation of the expression of ZFAS1 and miR-372-3p with the prognosis of patients; COX regression was used to analyze the risk factors for poor prognosis of lung cancer patients. Results Compared with the situation of adjacent tissues, the expression of ZFAS1 and miR-372-3p in tumor tissue was significantly higher (P<0.05). The expression of ZFAS1 and miR-372-3p in tumor tissue was related to TNM staging, lung membrane infiltration, whether lymph node metastasis occurred, and the degree of tumor differentiation (P<0.05). The expression of ZFAS1 and miR-372-3p content in lung cancer tissues was significantly and positively correlated (r=0.477, P<0.001). The 5-year survival rate of patients in the ZFAS1 and miR-372-3p high expression group was significantly lower than the low expression group (P<0.05). TNM staging, lymph node metastasis, ZFAS1, and miR-372-3p were independent risk factors for poor prognosis in patients with lung cancer(P<0.05). Conclusions ZFAS1 and miR-372-3p are highly expressed in lung cancer tissues and the expression level is related to the malignant characteristics and prognosis of lung cancer patients, so it may be regarded as an independent risk factor to predict poor prognosis of lung cancer patients.
    Resveratrol alleviates LPS-induced heart injury in mouse models
    ZHENG Ling, CHEN Li, WANG Yue
    2022, 42(12):  1867-1872.  doi:10.16352/j.issn.1001-6325.2022.12.1867
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    Objective To investigate whether resveratrol (RES) can alleviate lipopolysaccharide (LPS)-induced heart damage in mice. Methods Mice were randomly divided into control group, LPS group(intraperitoneal LPS 7.5 mg/kg) and RES low(20 mg/kg) and high-dose(60 mg/kg) intervention groups. The cardiac function was examined by ultrosound method; heart tissue sections was microscopied with HE staining for morphological change and apoptosis of heart tissue; Western blot was used to examine the expression of the apoptosis-related proteins Bax and Bcl-2. Microplate assay was used to detect CK-MB and LDH in serum; ELISA was performed to detect the proinflammatory cytokines IL-1β, IL-6 and TNF-α levels in serum and a commercially available kit was used to detect SOD viability and MDA content in heart tissue homogenates. Results Compared with the control group, LPS group mice showed a decreased cardiac function(P<0.05) and morphological change of tissue(P<0.05), significant increase of CK-MB, LDH, IL-1, IL-6, TNF-α release(P<0.05), decreased SOD activity(P<0.05), increased MDA content(P<0.05), increased myocardial apoptotic cells and Bax to Bcl-2 ratio(P<0.05) were recorded. The RES intervention significantly reversed these changes (P<0.05), with a dose-dependent manner. Conclusions RES alleviates LPS-induced cardiac damage through an anti-inflammatory,antioxidant and anti-apoptotic mechanism.
    miR-499a-5p alleviates hypoxic damage of pheochromocytoma cells through targeting at Pten
    ZHANG Li-yang, SUN Jun, CHEN Di
    2022, 42(12):  1873-1878.  doi:10.16352/j.issn.1001-6325.2022.12.1873
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    Objective To study the effect of miR-499a-5p on injury induced by chemical hypoxia of pheochromocytoma cell(PC12). Methods PC12 cells were divided into control group, hypoxia group (treated with CoCl2 for 24 h), hypoxia+miR-NC or miR-mimic groups. Cell viability was detected by MTT assay; the expression of miR-499a-5p was detected by RT-qPCR; lactate dehydrogenase (LDH) activity was detected by kit; apoptosis was detected by flow cytometry; the expression of phosphatase and tensin homolog (PTEN) protein was detected by Western blot. The luciferase reporter gene was used to detect whether Pten was a direct target of miR-499a-5p. Results Compared with control group, the cell viability and miR-499a-5p expression decreased (P<0.01 or P<0.001), LDH activity and apoptosis increased (P<0.01 or P<0.001) in hypoxia group. Over-expression of miR-499a-5p enhanced survival rate and inhibited apoptosis of PC12 cells under hypoxia (P<0.05 or P<0.01). Pten was a direct target of miR-499a-5p. Conclusions miR-499a-5p alleviates hypoxic injury of PC12 cells through targeting at Pten.
    Inhibition of p-caveolin1 attenuates neurological injury induced by cerebral ischemia-reperfusion in rats
    CHEN Ning-ning, YING Xin-wang, XIE Qing-feng
    2022, 42(12):  1879-1884.  doi:10.16352/j.issn.1001-6325.2022.12.1879
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    Objective To explore the mechanism of phosphorylation of caveolin1 (Cav1) in neurological injury after cerebral ischemia-reperfusion in rats. Methods The rats were randomly divided into sham group, model group and inhibitor group(PP2). Neurological function was evaluated by modified neurological deficit score (mNSS) neurobehavioral score, cerebral infarction volume was evaluated by triphenyltetrazolium chloride (TTC) staining, histomorphology after cerebral ischemia-reperfusion was evaluated by Nissl staining, and apoptosis of peri-infarcted tissue was detected by Tunel staining. Western blot and immunofluorescence were used to detect the protein expression of p-Cav1, glial fibrillary acidic protein (GFAP) and ion calcium binding junction molecule 1 (Iba1) in the tissue around cerebral infarction. Results Compared with the sham operation group, there were significantly increased mNSS score, volume of cerebral infarction, number of apoptotic cells and expression of p-Cav1, GFAP and Iba1 in the model group,while the number of Nissl corpuscles decreased. The inhibitor PP2 reduced the changes of the above-mentioned indexes and protect the neurological function of rats with cerebral ischemia-reperfusion injury. Conclusions Inhibition of p-Cav1 may improve the neurological function after cerebral ischemia-reperfusion in rats with potential mechanism of inhibiting glial cell activation and interaction.
    Screening of strategies for HA protein vaccine for influenza virus H1N1
    ZHENG Ning-chen, YANG Jiao-jiao, ZHANG Ting, WANG Zhi-rong, XU Xue-mei
    2022, 42(12):  1885-1890.  doi:10.16352/j.issn.1001-6325.2022.12.1885
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    Objective To screen and establish the optimal expression strategy of influenza virus H1N1 A/Nebraska/14/2019 HA, and lay a foundation for the development of protein vaccines. Methods The recombinant baculovirus of HA full-length, different C-terminal mutants of the extra cellular regions and co-expressing HA/M1 were constructed respectively. After 80 hours of infection in insect cells, the culture supernatant was collected, and the expression of recombinant antigen was identified by Western blot. Hemagglutination test indirectly analyzes its immunogenic. Then, HA/M1 protein was expressed in large quantities, and the physicochemical properties, hemagglutination activity and stability were analyzed after Core700 purification. Results The two C-terminal mutants of the extra cellular region of HA were all expressed in the form of trimers, but the expression supernatants had no hemagglutination activity; the full-length HA trimers had good hemagglutination activity, but they were membrane proteins thus were difficult to be purified. M1/HA co-expression supernatant also had hemagglutination activity. Electron microscopy of expression product purified by Core700 showed virus-like particles (VLPs) of 80 nm~150 nm. The hemagglutination titer of HA-M1-VLP reached 26 and was stable for three months at 4 ℃ as shown by light scattering. Conclusions HA-M1-VLP is easy to express and to be purified. The product has good stability, and hemagglutination activity, so can be used for the research of influenza virus protein vaccine.
    Correlation between susceptibility to chronic obstructive pulmonary disease and single nucleotide polymorphisms
    XIA Cen-feng, WANG Na, ZHANG Peng
    2022, 42(12):  1891-1894.  doi:10.16352/j.issn.1001-6325.2022.12.1891
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    Objective To investigate the correlation between single nucleotide polymorphism (SNP) and susceptibility of chronic obstructive pulmonary disease (COPD). Methods A total of 444 COPD patients and 434 healthy people were selected as the research objects. The Sequenom MassARRAY typing was performed at four loci of chitotriosidase-1(CHIT1): rs771576027, rs1402870472, rs61745299 and rs527497633, and a site rs117292391 on phosphodiesterase 4D(PDE4D). The correlation between single nucleotide polymorphism (SNP) and the risk of COPD was statistically evaluated. Results Genotype CG and allele C of rs61745299(CHIT1) increased the risk of COPD, and the distribution frequency was significantly different (P<0.05). Genotype AG and allele G of rs117292391(PDE4D) also increased the risk of COPD with significant, distribution frequency(P<0.05). Smoking also increased the risk of COPD. Conclusions rs61745299(CHIT1) and rs117292391 (PDE4D) are potential candidates of COPD biomarkers.
    IL-6 trans-signaling inhibitor sgp130Fc suppresses inflammation and regulates immune cells in mice with sepsis
    SHI Dan-dan, JIANG Su-fang, WANG Qian, ZHAO Peng-bo, LIU Ya
    2022, 42(12):  1895-1899.  doi:10.16352/j.issn.1001-6325.2022.12.1895
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    Objective To investigate effects of IL-6 trans-signaling on immuno-inflammatory regulation in cecum ligation and puncture (CLP)-induced mice with sepsis. Methods Eighty mice were divided randomly into four groups: sham group (n=15), CLP group (n=30), CLP+sgp130Fc group (n=20) and sgp130Fc group (n=15). Sgp130Fc 0.5 mg/kg was injected intraperitoneally one hour after CLP in the CLP+sgp130Fc group and sgp130Fc group. Body weight and survival rate of mice were monitored daily during the research period (10 days). At 24 hrs after CLP, IL-6,IL-10, MCP-1 and TNFα were detected by ELISA; Peripheral blood leukocytes were separated and lymphocyte subgroups were sorted with the flow cytometry. Results Compared with sham group, the survival rate and body weight decreased significantly(P<0.05). At 24 hrs after CLP, the expression of IL-6, IL-10, MCP-1 and TNFα increased significantly(P<0.05). Lower level of the CD3+ CD4+ T cell and CD45+ CD19+ B cell but higher level of the CD3+ CD8+ T cell in the CLP group were found (P<0.05). Compared with the CLP group, the survival rate was increased significantly(P<0.05). At 24 hrs after CLP, the expression of IL-6, IL-10, MCP-1 and TNFα decreased significantly, and higher level of CD3+ CD4+ T cell and CD45+ CD19+ B cell but lower level of CD3+ CD8+ T cell in the CLP+ sgp130Fc group(P<0.05) were recorded. Conclusions Inhibition of IL-6 trans-signaling can reduce mortality of mice with sepsis, which is potentially related to the regulation of early peripheral blood lymphocyte subgroups and inhibition of inflammatory responses.
    Clinical Sciences
    Reason analysis for unplanned discontinuation of patient-controlled intravenous analgesia after thoracic surgery
    WANG Jing-yi, CHEN Si, ZHANG Yue-lun, WANG Ying-li, REN Li-ying, SHEN Le
    2022, 42(12):  1900-1905.  doi:10.16352/j.issn.1001-6325.2022.12.1900
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    Objective To analyze the impact factors for unplanned discontinuation of patient-controlled intravenous analgesia (PCIA) within 24 h after thoracic surgery so as to prevent over-use of medical resources. Methods Clinical data of patients with thoracic surgery in Peking Union Medical College Hospital from April 1, 2018 to March 31, 2019 were reviewed. Patients were divided into two groups according to the duration time from the start to the end of PCIA: group A(≤24 h) and group B(>24 h). Univariate analysis and logistic multivariate regression were used to compare the demographic characteristics, anesthesia/surgery type, PCIA regimen and adverse effect between the two groups. Results A total of 371 patients were included in this study. Univariate analysis showed that the proportion of patients stopped PCIA in 24 hours was higher in female patients than that in male patients (16.8%/39.4%). The proportion of PCIA stop in 24 hours after thoracoscopic surgery was higher than that after thoracotomy (31.4%/19.7%). In PCIA, patients who used morphine were more likely to stop using postoperative PCIA within 24 hours than those who used oxycodone and sufentanil, and patients who used oxycodone were more likely to stop using PCIA within 24 hours than those who used sufentanil (60%/40.5%/25.3%). The proportion of patients who stopped using 5-HT3 receptor blocker in PCIA within 24 hours was lower than that of those who did not (14.9%/30.6%). Patients who were of sedation at the time of follow-up were less likely to stop using PCIA within 24 hours than those who did not have sedation (25.3%/41.8%). Patients who complained of postoperative nausea were more likely to stop using PCIA within 24 hours than those who did not(62.1%/17.3%).The proportion of patients with postoperative vomiting who stopped using PCIA within 24 hours was higher than those without postoperative vomiting (64%/22.1%). Logistic multivariate analysis showed that female, thoracoscopic surgery, intraoperative inhalation of nitrous oxide, postoperative nausea and postoperative vomiting were related with unplanned discontinuation of PCIA within 24 h after thoracic surgery. Conclusions For patients undergoing thoracic surgery,the application of postoperative PCIA should be comprehensively evaluated according to the patient's personal situation, surgery type, and the risk of postoperative nausea/vomiting.
    Combined detection of biomarkers of cartilage metabolism for early diagnosis of knee osteoarthritis
    HU Nan, ZHANG Jing, WANG Jing, WANG Pei, WANG Jing, LI Zi-cheng, WU Tian-ming, WANG Ya-hong, WANG Qiang, HE Lan
    2022, 42(12):  1906-1910.  doi:10.16352/j.issn.1001-6325.2022.12.1906
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    Objective To evaluate the clinical significance of biomarkers of cartilage synthesis and degradation for early diagnosis and prognosis of knee osteoarthritis (OA). Methods Two hundred and twenty-seven knee OA outpatients and inpatients in Shenmu Hospital were collected as the knee OA group, while 178 healthy physical examiners as the control group during the same period. Serum pro-collagen ⅡA N-teminal peptide (PⅡANP),cartilage oligomeric matrix protein (COMP), matrix metalloproteinase (MMP-3), and urinary cross-linked C-telopeptide (CTX-Ⅱ)were measured by enzyme-linked immunosorbent assay. The degree of knee OA involvement and quality of life were assessed by antero-posterior and lateral radiographs of both knees and the SF-36 brief health questionnaire, respectively. Results 1)Compared with the control group, the serum PⅡANP level decreased and serum COMP, MMP-3 as well as urinary CTX-Ⅱ increased in the knee OA group (P<0.05). 2)Serum PⅡANP was lower (P<0.05), serum COMP, MMP-3(P<0.05) and urinary CTX-Ⅱ levels were higher in mid-late stage knee OA group than early knee OA group and control group. 3)Serum PⅡANP levels were inversely correlated with K-L grade (P<0.05), serum COMP and MMP-3 levels were positively correlated with K-L grade (P<0.05), and serum COMP level was positively correlated with age (P<0.05). Serum PⅡANP level was negatively correlated with mental health dimension in the SF-36 scale (P<0.05); serum COMP level was negatively correlated with physical function dimension (P<0.05) but positively correlated with mental health dimension (P<0.05). Conclusions The expression of PⅡANP, COMP, MMP-3 and CTX-Ⅱ is abnormal in knee OA and related to the progress of the severity of the disease, so is a potential reference index for the diagnosis and prognosis of knee OA.
    Osteoporosis and influencing factors in adult women of Inner Mongolia
    HUI Chun-xia, SHANG Shu, QIAN Yong-gang, LU Ai-tao, GUO Lin, LI Na
    2022, 42(12):  1911-1915.  doi:10.16352/j.issn.1001-6325.2022.12.1911
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    Objective To explore the prevalence of osteoporosis in adult women in Inner Mongolia Autonomous Region and to analyze the influencing factors. Methods Totally 1 333 adult women from 4 regions of Inner Mongolia were selected by stratified cluster random sampling method from July 2014 to December 2015. Questionnaire survey, physical examination and laboratory testing were conducted. Calcaneal bone mineral density was measured by dry ultrasound bone mineral density analyzer. Univariate and multivariate Logistic regression were used to analyze the influencing factors of osteoporosis in adult women. Results The prevalence of osteoporosis in adult women in Inner Mongolia was 7.88% (standardized rate 8.56%). Logistic regression analysis showed that the protective factors of osteoporosis prevalence included young age, high intensity of physical exercise and high height (P<0.05), while menopause was an independent risk factor for osteoporosis (P<0.05). Conclusions The osteoporosis of adult women in Inner Mongolia is closely related to age, physical exercise, menopause and body height.
    Hyaluronic acid medical dressing promotes skin barrier repair after Picoway laser treatment
    CHEN Feng-juan, KE Jin, QIAN Li-jie, MA Wei-wei, WANG Xiong
    2022, 42(12):  1916-1920.  doi:10.16352/j.issn.1001-6325.2022.12.1916
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    Objective To study the efficacy and safety of hyaluronic acid medical wound dressing on skin repair after Picoway laser treatment. Methods Thirty patients after Picoway laser treatment were randomly divided into medical wound dressing group and control group. Medical wound dressing group was treated with medical wound dressing combined with the Danrushuang made by Shanghai Dermatology Hospital immediately after treatment, while the control group was treated with the Danrushuang made by Shanghai Dermatology Hospital alone for 7 days. Results The quantitative evaluation results of skin image analyzer (VISIA-6) showed that the improvement of redness area, texture and brown spots in the medical wound dressing group was better than that in the control group(P<0.05) after 7 days treatment. CK skin tester (MPA-580) showed that the skin water content of the medical wound dressing group was obviously higher than that of the control group at different time points after laser treatment, and the transepidermal water loss at each time point was significantly lower than that of the control group(P<0.05). There was no significant adverse events recorded in all patients. Conclusions The use of hyaluronic acid medical wound dressing after Picoway laser treatment may promote the recovery of the skin barrier, alleviate acute inflammatory reaction and have an obvious moisturizing effect.
    Mini Reviews
    Research progress on the role of winter swimming in the pathogenesis of acute myocardial infarction
    SHI Yang, BI Xiao-lei, SHAO Yi-bing
    2022, 42(12):  1921-1924.  doi:10.16352/j.issn.1001-6325.2022.12.1921
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    Incidence rate and mortality rate of acute cardiovascular disease(CVD), especially acute myocardial infarction(AMI)are significantly higher in cold environment. This may be closely related to sympathetic activation, blood viscosity change, coagulation activation, oxidative stress, inflammatory response and other factors affecting plaque stability by cold stimulation. Winter swimming is a kind of sports exposed to extreme cold pressure. People who often engage in winter swimming will have different degrees of cold adaptation, which may provide some protection to cardiovascular system against cold exposure.
    Research progress on the role of bilirubin in inflammatory bowel disease
    SHI Li, YUAN Hao, LIU Min, ZHENG Ya, WANG Yu-ping, GUO Qing-hong
    2022, 42(12):  1925-1929.  doi:10.16352/j.issn.1001-6325.2022.12.1925
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    The role of bilirubin in inflammatory bowel disease is not only functioned as an antioxidant and anti-inflammatory factor, but also functioned in immune regulation, anti-platelet aggregation, and as inhibition factor of digestive protease and remodeling of microbiota. Serum bilirubin level is correlated with disease activity and severity, so is a potential serum marker of inflammatory bowel disease in terms of supporting clinical diagnosis and treatment of inflammatory bowel disease.
    Research progress of microRNAs as remodelers of lipid metabolic reprogramming in hepatocellular carcinoma
    WANG Yu-min, CHEN Ji-chao, WANG Hong-quan, WANG Jin-hua, TANG Bo
    2022, 42(12):  1930-1934.  doi:10.16352/j.issn.1001-6325.2022.12.1930
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    Metabolic reprogramming is considered to be one of the top characteristics of tumor cells. Ample experimental studies have shown that microRNAs, which are a group of multifunctional non-coding RNAs, play an important role in the development of hepatocellular carcinoma (HCC) through lipid metabolic reprogramming. The current review briefly introduces the recent progress of specific microRNAs targeting at lipid metabolic reprogramming in HCC, which may provide a potential strategy for clinical targeting tumor therapy.
    Research progress on STAT3 and pathogenesis of Parkinson's disease
    WU Chang-bao, HAO Yan-lei
    2022, 42(12):  1935-1938.  doi:10.16352/j.issn.1001-6325.2022.12.1935
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    Parkinson's disease (PD) is a chronic progressive neurodegenerative disease. Signal transducer and activator of transcription 3 (STAT3) is involved in the development and progression of PD, oxidative stress, neuro-inflammatory response, abnormal α-synuclein aggregation, mitochondrial dysfunction and iron death, so they are expected to be a new therapeutic targets and provide new research ideas for the treatment of PD.
    Progress of CD8+CD25+regulatory T cells in diseases
    WEN Li-na, CHEN Li, DUAN Rui, LI Fang
    2022, 42(12):  1939-1944.  doi:10.16352/j.issn.1001-6325.2022.12.1939
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    CD8+CD25+Treg can inhibit immune response and induce immune tolerance. This article reviews the changes and underlying mechanisms of CD8+CD25+Treg in pathogenesis of malignant tumors, autoimmune diseases and other common diseases such as asthma, allergic diseases, type 2 diabetes, and pre-eclampsia. The changes of CD8+CD25+Treg in different diseases are inconsistent, cell counting increased in malignant diseases but decreased in autoimmune diseases. Further understanding of CD8+CD25+Treg would supporte a basis for the prevention, treatment and improving prognosis of these diseases.
    Advances in research of Blau syndrome
    KOU Yu-hui, YE Cai-ying, XING Cheng-feng
    2022, 42(12):  1945-1949.  doi:10.16352/j.issn.1001-6325.2022.12.1945
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    Blau syndrome is a rare auto-inflammatory disease caused by NOD2 mutation and a persistent hyper-inflammatory reaction mediated by transcription factor (NF-κB). The disease is characterized by an early onset of age and non-casein-like granulomatous inflammatory responses. The typical clinical manifestations are granulomatous dermatitis, symmetric arthritis, and uveitis. At present, the treatment of Blau syndrome is mainly based on glucocorticoid and immuno-suppressants, but the therapeutic effect is still not satisfactory.
    Research progress of oxidative stress increasing the risk of cardiovascular disease of polycystic ovary syndrome patients
    LI Huan-huan, ZHANG Yu-qi, LYU Hai-hong
    2022, 42(12):  1950-1954.  doi:10.16352/j.issn.1001-6325.2022.12.1950
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    Polycystic ovary syndrome (PCOS) is related to obesity, hyperinsulinemia, impaired glucose toler-ance, hyperandrogenism, chronic inflammatory response, dyslipidemia and hypertension. PCOS is critically associated with oxidative stress (OS), which is a risk factor for cardiovascular disease (CVD). This article reviews the mechanisms by which OS increases the risk of CVD in PCOS and the possibility of preventing CVD in PCOS patients by blocking the pathway of reactive oxygen species (ROS) production.
    Research progress of transcription factor AP-2 γ in malignant tumors
    CHEN Kang, CHENG Fan
    2022, 42(12):  1955-1959.  doi:10.16352/j.issn.1001-6325.2022.12.1955
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    Transcription factor AP-2γ regulates not only the genetic procedures that control the proliferation and differentiation of trophoblast cells during early embryonic development, but also is the key target in the development of breast cancer, lung cancer, testicular cancer, melanoma and pancreatic cancer, thus affecting the occurrence and development of tumors. Therefore, paying attention to the regulatory mechanisms of AP-2γ in the occurrence and development of malignant tumors may provide new ideas for the diagnosis and treatment of tumors.
    Research progress on the role of gastric microecology in gastric carcinogenesis
    JI Ruo-yu, CAO Xin-yuan, ZHANG Yi-zhen, WANG Qiang, WU Xi, YANG Ying-yun, YANG Ai-ming
    2022, 42(12):  1960-1964.  doi:10.16352/j.issn.1001-6325.2022.12.1960
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    Human stomach has a unique microbial ecosystem, and the disruption of its homeostasis is closely associated with the development of gastric cancer (GC). Gastric carcinogenesis is correlated with the dysbiosis of gastric mucosal microbiota, which is characterized by the decrease of biologigical diversity and alterations of the composition. The microbiota dysbiosis further induces metabolic disorders and triggers the formation of tumor microenvironment in the stomach which contributes to the development and progression of GC. Exploring the role of gastric micro-ecology in gastric carcinogenesis may provide new insights into the diagnosis and treatment for GC.
    Medical Education
    Application of scenario simulation teaching in pediatric internship of eight-year program medical students
    TANG Xiao-yan, QUAN Mei-ying, LI Ji, ZHAO Jun, LI Zheng-hong, SONG Hong-mei
    2022, 42(12):  1965-1967.  doi:10.16352/j.issn.1001-6325.2022.12.1965
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    Objective To evaluate the impact of scenario simulation teaching on the training of pediatric internship for eight-year program medical students. Methods Sixty medical students were enrolled in the study. The month spend in pediatric department was divided into traditional teaching for two weeks and scenario simulation teaching for another two weeks. Electronic questionnaires and anonymous surveys were applied to all medical students, and then learning effects were compared. Results The response from 64.71% of students showed great interests on the scenario simulation method which improved the enthusiasm for learning. The results also showed that 58.82% of students acknowledged the great support to their training. Also,74.51% of students agreed that scenario simulation teaching promoted their clinical practice. However, the implementation of scenario simulation teaching often required students to spend more time. Conclusions Scenario simulation teaching can be used as an important assistant teaching mode in the process of pediatric internship training and it should be adopted in combination with the in spot training.
    Analysis of consultation in orthopedic department of comprehensive grade A hospital and its guiding significance to clinic and teaching
    LIU Sen, WANG Wei, YUAN Tang-mi, ZHANG Jian-guo, YANG Bo
    2022, 42(12):  1968-1972.  doi:10.16352/j.issn.1001-6325.2022.12.1968
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    Objective To evaluate the time span, space distribution, disease spectrum, professional and technical requirements of orthopedic surgeons for consultation in orthopedic department in a large comprehensive grade A hospital, so as to strengthen clinical teaching and improve the quality and efficiency of consultation. Methods The data of consultation received by the department of orthopedics in Peking Union Medical College Hospital from December 2020 to January 2022 were collected and sorted out by the hospital information system, and the correlation between consultation time span, department, consultation purpose, diseases involved, orthopedic treatment opinions, relevance of consultation to patients' main purpose of hospitalization was classified and summarized. Results In the statistical time range, a total of 1 528 consultations were completed by the department of orthopedics, involving 41 clinical departments. The consultation calling peak was from 8 am to 11 am and from 15 pm to 17 pm,and consultation on Monday was more than other days. The department of neurology submitted the most applications for orthopedic consultation, and the main diseases were degenerative diseases. There were also a large number of orthopedic disorders associated with hormone use. About 1/3 of the consultations were not directly related to the primary purpose of hospitalization. About 2/3 of the consultation cases required orthopedic intervention, while another 1/3 had incomplete preparation or only needed oral instruction. Conclusions The working load of in-hospital consultation can not be ignored. The time span and departments submitting the consultation application are unevenly distributed. The recommendation for consultants is to reserve sufficient time on Monday to ensure timely consultation. The consultation content mainly focuses on degenerative diseases and glucocorticoids related orthopedic diseases. In the future, the training of consulting physicians and the basic education as well as post-graduation education of common orthopedic diseases should be strengthened.