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Table of Content

    05 November 2015, Volume 35 Issue 11
    Construction of human recombinant KNDC1 adenovirus and its effect of promoting HUVEC senescence
    2015, 35(11):  1447-1452. 
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    Objective To construct the human recombinant KNDC1 adenovirus and to investigate the expression and function of KNDC1 protein in human umbilical vein endothelial cells (HUVECs). Methods Human KNDC1 gene was firstly amplified by PCR and directly cloned into the linearized adenoviral vector in vitro, followed by screening and gene sequencing. Finally, the linearized recombinant plasmid was transfected into HEK293A cells for packaging. PAdenoⅩ-KNDC1 was purified by pAdenoⅩ kits and the endpoint dilution method was used for titering recombinant pAdenoⅩ-KNDC1. For observing the expression level of KNDC1 proteins, pAdenoⅩ-KNDC1 was used to infect HUVECs and then examined by Western Blot. The function of pAdenoⅩ-KNDC1 was detected by senescence β-galactosidase staining kit. Results Gene sequencing indicated that pAdenoⅩ-KNDC1 contained KNDC1 gene. HEK293A cells turned ground and partially floated in the tenth day after transfection. The titer was 1×1011 PFU. Meantime, significant upregulation of KNDC1 protein expression in HUVECs at 48h after infection was observed. The number of senescence cells was increased with the increase of pAdenoⅩ-KNDC1 infection. Conclusion PAdenoⅩ-KNDC1 was successfully constructed and could promote the senescence of HUVECs.
    Effect of adenovirus-mediated AIF and its mutants on the apoptosis of K562 cells
    2015, 35(11):  1453-1459. 
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    Objective To construct the recombinant adenovirus Ad-AIF, Ad-DMLS-AIF and Ad-DHBD-AIF and detect their apoptotic effect on leukemia K562 cells. Methods AIF gene and its mutants(DMLS-AIF gene and DHBD-AIF gene)were amplified and cloned into the shuttle plasmid pAd -Track-CMV-HA in turn, after identification by PCR、double-enzyme digestion and sequencing, recombined homology with pAdEasy1 to obtain recombinant adenovirus plasmid and then which were packaged and amplified, adenovirus without exogenous sequence as empty vector. Western blot was used to analyze expression of the recombinant adenovirus in K562 cells; Co-immunoprecipitation assay (CO-IP) were applied to detect the relationship between HSP70 and AIF or DMLS-AIF or DHBD-AIF; Hoechst33258 dye and FCM were applied to detect the apoptotic effect of the recombinant adenovirus on K562 cells. Results The recombinant adenovirus were constructed successfully and expressed in K562 cells; AIF and DMLS-AIF were connected to HSP70 except DHBD-AIF; the chromatin were agglutinated in K562 cells infected with Ad-DHBD-AIF, that were uniformity in K562 cells infected with Ad-AIF or Ad-DMLS-AIF; the apoptotic effect of Ad-DHBD-AIF on K562 cells was higher than that of Ad-AIF and Ad-DMLS-AIF. Conclusion Ad-AIF、Ad-DMLS-AIF and Ad-DHBD-AIF were constructed successfully, the apoptotic effect of Ad-DHBD-AIF on K562 cells were higher significantly comparing with that of Ad-AIF and Ad-DMLS-AIF, which could be as the basis of the further experience of K562 cells anti-apoptosis.
    Cloning and expression of human DNA methyltransferase 3B isoform and its biological effects on human embryonic kidney cell line 293A growth
    2015, 35(11):  1460-1465. 
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    Objective To construct six kinds eukaryotic expression vectors of human DNA methyltransferase 3B isofroms and overexpress these recombinant DNMT3B isoforms, study its biological effects on human embryonic kidney (HEK) cell line 293A.Methods The human DNMT3B1 and DNMT3B2 were amplified from HCT116 using high fidelity Taq PCR. Others isoforms were amplified from DNMT3B1 or DNMT3B2. Then the PCR product were linked into eukaryotic expression vector pCMV-2B. These recombination plasmids were identified by double enzyme digestion with BamHⅠand ECORⅠand by sequence. These recombination plasmids were transfected into 293A cells when the sequencing results of recombination plasmids were correct. The G418 was used to get the stable cell line which express DNMT3B isofoms respectively. MTT assay was used to detect the proliferation of cells which stable express the DNMT3B isoform. Results six kinds of Human DNMT3B CDS sequence were cloned. The stable 293A cells overexpressing human DNMT3B isoforms were established. Overexpression DNMT3B3、DNMT3B4 and DNMT3B7 can inhibit the growth of 293A cells. Conclusion Eukaryotic expression vectors encoding human DNMT3B isoforms and corresponding stable overexpression cells were constructed. Different human DNMT3B isoforms protein may have different effects on 293A cell growth.
    HSG inhibits the proliferation of fibroblasts in COPD rats by Wnt / β-Catenin signaling pathway
    2015, 35(11):  1466-1470. 
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    Objective Research the relationship between HSG and Wnt/β-Catenin signal transduction pathway in airway remodeling in COPD,try to elaborate HSG gene regulation mechanism of airway remodeling.Methods rats were divided into normal control group and COPD model group.COPD model group were intratracheal injection of LPS plus smoked methods.Used HE staining to identify COPD model.Determination of the total area of the airway wall/airway basement membrane perimeter(WAt/Pbm)evalution of airway remodeling situation.Cultured the rat airway primary fibroblasts cells in vitro by tissue blocking adhering method.Realtime-PCR were used to detect the HSG and β-catenin mRNA expression in fibroblasts.Used Western blotting assay and ELISA for HSG,β-catenin、GSK-3β,TGF-β1,MMP-9 in small airway fibroblasts.Explored the correlation analysis of HSG,β-catenin with TGF-β1,MMP-9.Results Model group rats small airway remodeling was significantly.COPD model group airway fibroblasts HSG mRNA,protein expression were lower than the normal group. mRNA,protein expression of β-catenin were higher than normal group.Expression of P-GSK-3β,TGF-β1 and MMP-9was also higher than the normal group.HSG mRNA expression and mRNA expression of β-catenin and TGF-β1, MMP-9 secretion of cytokines were negatively correlated.Conclusion The HSG gene might regulated Wnt/β-catenin signaling pathway,whcih resulted in inhibiting the fibroblasts proliferation.
    The changes of immune cell populations in peripheral blood of mice with lymph node metastasis of pancreatic cancer
    2015, 35(11):  1471-1475. 
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    Objective To observe the changes of immune cell populations in peripheral blood of mice with lymph node metastasis of pancreatic cancer. Methods C57BL/6J mouse syngeneic Panc02 pancreatic cancer cells were injected into intra-footpad . The FCM (fluorescence activated cytometry) was adopted to detect 13 immune cell populations in peripheral blood. Results Six weeks after injection, the lymph node metastasis rate was 65%. Compared to that of normal mice, in the peripheral blood of the mice with and without lymph node metastasis, the B lymphocytes(P<0.05,P<0.01) and T lymphocytes (P<0.05,P<0.01)were significantly decreased; however, the granulocytes(P<0.05,P<0.01), myeloid derived suppressor cells (MDSC) (P<0.01,P<0.01)and M2 polarized tumor associated macrophages (TAM) (P<0.05,P<0.01 were significantly elevated. Compared with the tumor bearing mice without lymph node metastasis, in the peripheral blood of the tumor bearing mice with lymph node metastasis, the changes of the above immuen cell populations were more obvious(P<0.05). Conclusion Pancreatic cancer with lmyph node metastasis can induce intensively systemic immunosuppression and the MDSC and M2 polarized TAM may play crucial roles. Immune reconstructions are potential important targets for the prevention and treatment of lymph node metastasis of pancreatic cancer.
    Effects of the polymorphisms in MEF2A promoter on the transcription activity and the susceptibility to coronary artery disease
    2015, 35(11):  1476-1480. 
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    Objective To explore the influence of the polymorphisms in the promoter of myocyte enhancer factor 2A (MEF2A) on the promoter activity, and the relationship of the polymorphisms with the susceptibility to coronary artery disease (CAD). Methods Forty-four CAD patients and 45 controls were enrolled. The single nucleotide polymorphisms (SNPs) in the promoter of MEF2A and the promoter haplotypes were identified with cloning and Sanger DNA sequencing. The transcription activity of the different promoter haplotypes were determined with dual luciferase reporter system. The frequency of the haplotypes and the homozygosity were compared between CAD and control group. Results Nine SNPs were found, and they combined into 18 haplotypes. H1(16.29%)、H5(17.42%)、H8(16.85%) and H16(28.65%)are the most popular haplotypes, and take 79.21% of the total. H5 and H8 showed the highest transcription activity, and H9 was next, the others were relative weak. Three SNPs, -580(C -> A), -646(G -> T) and -948(C -> T), cause a loss of predicted transcription factor binding site (TFBS). The haplotypes clustered with this 3 SNPs and the frequency was shown as follow: CGC(17.98%)、CGA(3.94%)、TGA(36.51%)、TTC(33.15%)、TTA(3.94%)、TGC(3.37%)、CTC(1.69%). The haplotype with TGA showed the strongest transcription activity. The homozygosity in CAD group is markedly higher than that in the control group (P < 0.05). Conclusion The SNPs in the promoter of MEF2A may lead to change of TFBS. The different haplotypes have distinct promoter activity, and the homozygosity of MEF2A promoter may contribute to a higher susceptibility to CAD.
    Regulation of dihydrotestosterone on the expression of GDNF in primary cultured rat Sertoli cell
    2015, 35(11):  1481-1485. 
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    Objective To study the effects of Dihydrotestosterone (DHT) on the expression of GDNF and the changes of signaling pathway in primary cultured rat Sertoli cells. Method the primary cultured 20 days old sertoli cells stimulateused by DHT, immumofluorescence was used to detect the localization and expression of GDNF in Sertoli cell, Western-blot to detect the expressions of GDNF, ERK1/2 and CREB , and the phosphorylations of ERK1/2 and CREB. while the inhibitors of ERK and cAMP/PKA signaling pathway were used. Result GDNF expressed in cytoplasm of Sertoli cell. DHT promoted the expression of GDNF, while the levels of ERK1/2 and CREB phosphorylation were increased. U0126 and H89 decreased the GDNF expression induced by DHT. Conclusion DHT stimulated the expression of GDNF of Setoli cell by activing the ERK signaling pathway.
    The Physiological Characteristics and the Most Common Diseases in the People Living in the Region from North Latitude 53°27′40″ to 53°29′30″
    2015, 35(11):  1486-1491. 
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    Objective To explore the physiological characteristics and the most common diseases in the people living in the region from North Latitude 53°27′40″to 53°29′30″. Methods A whole sampling design method was used to a population living at the region from North Latitude 53°27′40″to 53°29′30″. All people were included in this study.The inclusion criteria were that all participants were 18 years or older, they were descendants of at least three generations living in the same region, and there was no history of intermarriage. Interviewers administered a simple questionnaire to collect demographic and behavioural risk data. The anthropometric parameters were obtained by the medical examination. The prevalence of some common diseases(such as hypertension, obesity, diabetes, etc). Results 1)1064 people were included in this study, of them, 489(45.96%) were males, mean age (48.8±13.5) year; 575(54.04%) were females, mean age (47.1±13.8) years. The proportion of excessive drinking(more than 4 days in a week, and more than 250g for each day) was 18.14%(193/1064) in the participants. The level of systolic blood pressure and diastolic blood pressure in males were higher than that in females, they were SBP(126±16)mmHg vs.(120±22) mmHg and(83±10)mmHg vs.(77±13) mmHg(P<0.001). The levels of TG, UA, AST, ALT and GGT were higher in males than that in females(P<0.01). Some common diseases: the prevalence of hypertension, obesity, hypertriglyceridemia, hypercholesterolemia and stroke was 22.84%, 19.68%, 18.74%, 17.12% and 4.63%, respectively. The prevalence of abnormal liver function was 26.41% in all participants. Conclusion The priliminary results showed that the main physiological characteristics are high prevalence of overweight/obesity and dyslipidemia, and common diseases are hypertention and stroke. Obesity and excessive drinking might be the main risk factors for cardiovascular disease high prevalence of cardiovascular and cerebrovascular diseases in this population.
    α-Synuclein amino-terminus induces MN9D cells mitochondrial membrane injuries
    2015, 35(11):  1492-1498. 
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    Objective: The purpose of this study is to exam the changes of mitochondrial membrane induced by overexpressing α-syn, α-syn /N, NAC domain and α-syn /C domain in MN9D cells. Methods: Transiently transfected various domains of α-syn gene into MN9D cells. The MN9D cell’s viability was observed by MTT assay and release of LDH respectively. The reactive oxygen species (ROS) level, mitochondrial permeability pore (mPTP) and mitochondrial membrane potential (ΔΨm) were examined by confocal image and flow cytometry, the morphology of mitochondria was examined by confocal image. Results: Overexpressing α-syn,α-syn/N,α-syn/delC could induce the decrease of cell viability. The LDH release was increased by α-syn,α-syn/N,α-syn/delC (p <0.01). A-syn,α-syn/N,α-syn/delC could increase the ROS level(p<0.001),decrease mitochondrial membrane potential (p <0.01)and increase the opening of mPTP(p <0.01).The result of confocal microscopy showed that 55% mitochondria of HEK293T cells overexpressing α-syn/N had so-called balloon phenotype. Conclusions: Amino-terminus of α-synuclein induces mitochondrial membrane injuries.
    The survey of handgrip strength of 8~80 years of general population in Han population in Shaanxi province
    2015, 35(11):  1499-1502. 
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    Objective: To understand the distribution of handgrip strength of 8~80 years of general population in Han population in Shaanxi province, to provide evidence of general population reference of grip strength. Methods: Data was collected by questionnaire and Jamar dynamometer in Shaanxi Province (Xi'an City, Hanzhong City, Hu County, and Qishan County) between June to July in 2014. The value of grip strength was for sex-age description. Results: Except 12~13 years (P=0.07), grip strength of men was larger than that of women in each age group (P<0.05). Men showed a rapid rise in childhood of grip strength, kept stable in adulthood, and then gradually declined like the parabolic. Women showed a gradually increasing of grip strength at first, and then declined smoothly. In men, the means of grip peaked at 20~29 years (41 kg), remained to 30~39 years, and declined after 40~49 years. In women, the means of grip peaked at 30~39 years (24 kg), remained to 40~49 years, and declined after 50~59 years. Conclusion: Grip strength of men was almost larger than that of women in each age group of 8~80 years of general population in Han population in Shaanxi province. By age increasing, there was a trend of increasing at first and declining subsequently.
    SULT2B1 interference inhibits the epithelial-mesenchymal transition of human hepatocellular carcinoma BEL-7402 cells
    2015, 35(11):  1503-1507. 
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    Objective To investigate the relationship of hydroxysteroid sulfotransferase 2B1 (SULT2B1) and epithelial-mesenchymal transition (EMT), exploring its possible mechanisms. Methods cells were divided into three groups: control group, negative control siRNA group and SULT2B1-siRNA group. LipofectamineTM 2000 was employed for transient transfection. The SULT2B1 interference efficiency in treated BEL-7402 cells was detected by real-time PCR and western blot assays respectively. The protein levels of zonula occludens-1 (ZO-1), vimentin and transcriptional factor ZEB1 in BEL-7402 cells with SULT2B1 interference were determined by western blot analysis. Results The mRNA and protein levels of SULT2B1 in treated BEL-7402 cells were decreased significantly as compared with the negative control group (P< 0.05). The protein level of ZO-1 was up-regulated significantly (P<0.05), while vimentin and the transcriptional factor ZEB1 were decreased in BEL-7402 cells with SULT2B1 interference (P<0.05),. Conclusion SULT2B1 interference can inhibit the epithelial-mesenchymal transition of BEL-7402 cells by down-regulating transcriptional factor ZEB1.
    IL-25 induces the expression of Th2 inflammatory factor by nuocyte in asthma of mouse
    2015, 35(11):  1508-1513. 
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    Objective To study the effect of Interleukin-25 on expressing Th2 inflammatory factor in asthma by nuocyte cells. Methods Nuocyte were collected from mouse spleen of OVA-induced asthma model by density gradient centrifugation. Hematoxylin-eosin staining and pulmonary function tests were determined OVA-induced asthma model to be established successfully from the pathology and Physiology; Samples were divided into 3 groups according to different intervention conditions: Interleukin-25 group; control group; anti-Interleukin-25 group. The expression of Interleukin-5 and Interleukin-13 proteins in nuocyte cells supernatant was detected by ELISA. The expression of Interleukin-5 and Interleukin-13 proteins in nuocyte cells was detected by Western blot. The expression of Interleukin-5 and Interleukin-13 mRNA was detected by RT-PCR. The number of nuocyte cells was detected by flow cytometry. Results The study showed that expression of Interleukin-5 and Interleukin-13 protein and related gene in Interleukin-25 group was higer than that in the control group or anti-Interleukin-25 group (P<0.05). Flow cytometry showed that the number of nuocyte cells in Interleukin-25 group was higer than that in control group or anti-Interleukin-25(P<0.05). Conclusions Interleukin-25 can promote the expressing Interleukin-5 and Interleukin-13 in asthma by nuocyte cells and promote the occurrence and development of asthma.
    Analysis on genetic traits of human adipose derived mesenchymal stem cells during in vitro culture
    2015, 35(11):  1514-1519. 
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    Objective Investigate the biological characteristics and genetic features alteration of human adipose derived mesenchymal stem cells (ADSCs) cultured in vitro, to provide theory basis and a new source of cells for the research on the safety of its clinical use. Methods Observe the shape of the 1st, 3rd, 5th, 7th, 10th, 14th and 15th generation of the ADSCs cultured in vitro using the serum free culture medium. Analyze their cell cycle and cell surface markers by Flow cytometry,karyotype were analyzed by the chromosome G—banding technology.Expression level of relevant gene and cytokine was Measured respectively by the Real-time fluorescence quantitative technique and ELISA.Results The growth rate of ADSCs began to slow down after extended to the 7th generation, and after the 10th generation, it slowed down significantly . The shape of cell remained fusiform, no significant change. Cell cycle analysis revealed that the percentage of G2phase and S phase cells remained stable. There was no obvious missing, translocation or dislocation in terms of karyotype. Measured gene expression level had a little difference change, and the cytokine secreted in culture medium remained stable Conclusion The adipose derived mesenchymal stem cells cultured in vitro using the serum free culture medium are safe before the 5th generation.
    Cordycepin enhances apoptosis of Eca109 cells induced by cis-platinum
    2015, 35(11):  1520-1525. 
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    Objective To investigate the mechanism of action and impact of apoptosis in Eca109 treated with cordycepin and cisplatin METHODS Divide Eca109 into four groups named control group,cordycepin,cisplatin and combined group which was treated with different concentrations of cordycepin,cisplatin and cordycepin(70μg/mL)and cisplatin(0.8μg/mL).The proliferation was measured by MTS assay. Apoptosis was observed by using Hoechst 33258 staining and flow cytometry. Western blotting was used to detect the expression of NF-κBp65,Bcl-2 and Bax. Enzyme linked immunosorbent assay(ELISA) was used to detct the binding activity with NF-κBP65 and DNA in cell nucleus.RESULTS The inhibition ratio induced by cordycepin and cis-platinum combination was increased from 29.3% to 70.4% in Eca109 cells(P<0.05). Cordycepin combined with cis-platinum can remarkablely promote apoptosis in Eca109 cells than using cisplatin alone.Cisplatin increased the activity of the NF-κBP65 in the nuclei while cordycepin can inhibit the activity of the NF-κB P65, the activity of the NF-κB P65 decreased with combined treatment of cordycep and cisplatin. Compared to cisplatin alone,cordycepin combined with cisplatin decreased the expression of Bcl-2 while remarkblely increased the expression of Bax. CONCLUSION Cordycepin could enhanced the apoptosis of Eca109 cells induced by cisplatin and regulated downstream signaling molecule expression of Bcl-2 and Bax by inhibiting the NF-κB pathway.
    Expressions of Survivin and Ki-67 in colorectal adenocarcinoma and its clinical significance
    2015, 35(11):  1526-1530. 
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    Objective To investigate the expressions of Survivin and ki-67 in the colorectal adenocarcinoma and its clinical significance. Methods The expressions of Survivin and ki-67 in 60 specimens of coloreetal adenocarcinoma tissues and 30 specimens of normal eolorectal tissues were detected using Immunohistochemical SP technique and in situ hybridization. Results The positive expression rates of Survivin and Ki-67 in colorectal adenoeareinoma tissues were 73.34%and 80.00%,respectively,while in normal colorectal tissues they were 10.00% and 6.67%.The difference was significant (P<0.01). The positive expression rates of Survivin mRNA in colorectal adenoeareinoma and normal tissues were 10.00% and 6.67%.Expression of Survivin was positively related with expression of Ki-67 in coloreetal adenocarcinoma tissues(P<0.05).Expression levels of Survivin and Ki-67 were correlated with the TNM staging,liver metastasis,the depth of tumor invasion,lymph node metastasis,and peritoneal micrometastasis(P<0.05).The patients with positive expression of Survivin and Ki-67 had poor progosis with lower one,three,and five year survival rates.Survivin,Ki-67,the TNM staging,liver metastasis,lymph node metastasis, and peritoneal micrometastasis were independent prognosis factors for coloreetal adenocarcinoma. Conclusion: Thethe high expression levels of Survivin and Ki-67 have a high correlation with the initiation and progression of colorectal adenocarcinoma, and they may be amolecular-biological indicators in forecasting the progress and prognosis of colorectal carcinoma.
    Therapeutic observation on the early integrated rehabilitation treatment of cerebral stroke patients
    2015, 35(11):  1531-1534. 
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    Objective: To investigate the effect of early rehabilitation treatment on the recovery degree of the limb function and the activity of daily life in stroke patients. Methods: Seventy-two patients with acute stroke were randomly divided into two groups (Early rehabilitation group 36 cases, later rehabilitation group 36 cases). Early rehabilitation group was treated with normalized rehabilitation therapy as well as medication. Later rehabilitation group was treated with medication in the hospital for 4 weeks. After nerve symptoms were stable and no further deterioration, the rehabilitation training was performed. Results: Compared with the prior treatment, the FMA score and the Barthel score of the early rehabilitation group increased significantly (P<0.05); The score of FMA and Barthel in the early rehabilitation group were significantly higher than those in the later rehabilitation group, and the difference was significant (P<0.05);The effect of language rehabilitation training in early rehabilitation group was more obvious than the later rehabilitation group(P<0.05), and the effective rate was 94.1%. Conclusions: Early implementation of standardized rehabilitation training can significantly improve limb motor function, daily living activity, language barrier recovery ability, and the quality of life.
    Carnosine Reduces H2O2-induced cellular Injury in N2a Cells
    2015, 35(11):  1535-1539. 
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    Objective To investigate the protective effect of carnosine in oxidative stress induced by H2O2 in N2a cells. Methods The N2a cells were divided into three groups: normal control; H2O2 group; carnosine group: carnosine was used in advance. Cell morphology was observed by phase-contrast microscope; immunohistochemistry and Western blot were applied to detect expression of AKT1 and FAS-L. Results Carnosine significantly increased cell proliferation. Compared with control group,cell morphology changed significantly, cell shrinkage, membrane thickened, foaming phenomenon in H2O2 injury group; In the carnosine protection group, carnosine significantly inhibited the H2O2-induced cellular injury, cell morphology obviously better than H2O2 group. Compared with the control group, the expression of the AKT1 down-regulated and Fas-L up-regulated in H2O2 group, but in the carnosine protection group, the expression of the AKT1 up-regulated and Fas-L down-regulated than H2O2 group (P<0.05). Conclusions Carnosine obviously reduced H2O2-induced cellular injury in N2a cells.
    Research progress on the competing endogenous RNA and tumorigenesis
    2015, 35(11):  1549-1553. 
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    MiRNA can reduce the stability of the messenger RNA or inhibit the translation of the messenger RNA by targeting its 3’-Untranslated Region(3’UTR),thereby negatively regulate the expression of the target gene .Nevertheless , some mRNAs;pseudogenes;long non-coding RNAs(LncRNA)and circRNAs can competitively combine with the same miRNAs by miRNA respone elements(MREs),removing or reducing the inhibition of genes targedted by the miRNAs and regulating the expression of the tagert genes .This mechanism is called competing endogenous RNA(ceRNA) hypothesis. In the process of tumorigenesis,mRNAs;pseudogene;LncRNA and circRNA can function as a ceRNA and promote or inhibit the occurrence of tumor by competiting to binding the same miRNAs that can promote or suppress tumorigenesis.
    The advance of the interaction between microRNA and lncRNA in cancer reseach
    Ye Zhu
    2015, 35(11):  1554-1558. 
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    microRNA (miRNA) are a class of small non-coding RNA molecules have expression mediated gene transcription regulatory functions, long non-coding RNA (lncRNA) is untranslated transcripts with the number of bases greater than 200 nucleotides, through a variety of mechanisms including gene imprinting, chromatin remodeling, cell cycle regulation exerts its biological function.It has been found there exist the interaction relationship between miRNA and lncRNA,and plays an important role for tumorigenesis,tumor development and treatment.
    The relationship between FGF10 and related diseases and its clinical application
    2015, 35(11):  1559-1562. 
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    Fibroblast growth factor 10 (FGF10) is one of the fibroblast growth factor(FGFs) family members,which is related to many diseases, including tumor and various organs deficiency.In addition, it has been demonstrated that FGF10 possessed some abilities to promote wound healing, the regrowth of hair , and the proliferation and differentiation of stem cells and to cure the facial telangiectasis.
    The policy and legal analysis of medical institutions held by social capital
    2015, 35(11):  1563-1566. 
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    Through the review of policy and law on the setting up of medical institutions by social capital of Chinese Central Government and the introduction of local legislation experience, this paper analyzes the status quo and existing problems, and explores policy and legal measures to promote the establishing of medical institutions by social capital.
    Progress in the detection and targeted therapy of epidermal growth factor receptor variant III in ovarian cancer
    2015, 35(11):  1567-1571. 
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    The epidermal growth factor receptor variant III (EGFRvIII) also known as the most common mutant form of EGFR is a tumor-specific mutation, hasn’t yet been detected in normal tissues. It is regarded as a tumor-specific gene and offers a new way for targeted therapy of ovarian cancer.
    Current status of critical thinking and the co-relationship between critical thinking and the scores of preclinical courses on year 2 medical students
    2015, 35(11):  1572-1575. 
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    Objectives To investigate current status of critical thinking on year 2 medical students and explore the relationship between critical thinking and the scores of preclinical courses. Methods Chinese version of Critical Thinking Disposition Inventory (CTDI-CV) was used to investigate the current status of critical thinking on year 2 medical students in Peking University, correlation was analyzed between critical thinking and the scores of preclinical courses. Results The average total score of critical thinking ability of year 2 medical students in Peking University was 306. All seven CTDI-CV dispositional subscales were positive, scores of the inquisitiveness subscale and the analytic subscale were higher and the CT self-confidence subscale and the truth-seeking subscale were lower. Scores of preclinical courses had significant positive correlation with CTDI-CV and the open-mindedness subscale. Conclusions The results suggest that critical thinking disposition and the open-mindedness subscale should be improved in the medical education.
    An exploration on paperless examination system the practice of objective structured clinical examination at Peking Union Medical College
    2015, 35(11):  1576-1578. 
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    Objective structured clinical examination plays an increasing role in medical education nowadays, while its disadvantage of organizing is obviously, it needs much more resource. Its complex organizational processes bring a lot of tedious examination affairs for administers who in charge of OSCE, also it is not conducive to achieve standardization. A paperless examination system was established to address this problem at Peking Union Medical College. The system has its advantages and disadvantages. To address its disadvantages, 1-2 sets of solutions should prepared to deal with problems when it happens.
    The experience of teaching and learning histology and embryology in English: for medical undergraduates 2013
    2015, 35(11):  1579-1582. 
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    To meet the requirement for international medical professionals, histology and embryology was taught in English to the medical undergraduate students of year 2013 in Shenzhen University Health Science Center. This article not only addressed the problems and solutions in the relevant teaching and learning activities, but also analyzed and summarized the teaching results. In conclusion, histology and embryology was efficiently taught in English in the medical school of Shenzhen University.
    Problems and countermeasures of practice teaching in medical clinical
    2015, 35(11):  1583-1585. 
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    The clinical practice training plays an important role for medical students to be a clinical doctor. In recent years the main problems in the process of medical clinical practice are that short of enthusiasm about teaching of teachers, not strong practice of consciousness of students, emergence of the new physician-patient relationship, imperfect of the departmental rotation examination system and so on. The article discusses the causes of these problems, and puts forward some corresponding countermeasures, in order to further improve the level of medical clinical practice.
    Research and practice of scope management of hospital information system
    2015, 35(11):  1586-1590. 
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    Contraposing the current hospital information system characteristics and construction process of weak scope management situation,In this paper, the information system project management as the theoretical basis, the process of thinking being introduced into the scope management.The whole scope management process is divided into four stages: collect requirements,define scope, draft WBS and control scope. As a basis for the construction of hospital information system project scope management model. And proposed the stakeholder analysis method, the decomposition sub project method, the formulation of the change control process and the use of the method of change analysis software.Thus the effective management and control of project scope, to ensure the project on schedule according to the quality of the completion.