Basic & Clinical Medicine ›› 2015, Vol. 35 ›› Issue (12): 1633-1639.
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Abstract: Objective: To explore the effect of small molecule noncoding RNA(miRNA-95) on the invasion and migration ability of colon cancer SW620 cell line and its mechanism.Methods: The SW620 cells were infected by miR-95 interference lentivirus.The expression of miR-95 mRNA was confirmed by quantitative real-time polymerase chain reaction.Transwell chambers, scrath test were conducted to detect the ability of cell invasion,migration among each group;and MTT method was used to detect the intercellular adhesion of homogeneous and heterogeneous cells;Western blot was used to detect the expression of EMP1,VEGFC and MMP2 proteins.Results: After the infection of SW620 with miR-95 interference lentivirus for 72h,significangt fluorescence expression was observed.Compared with empty virus group and ccontrol group,the expression of miR-95 was decreased in transfection group (P<0.05);The invasion and migration ability weakened (P<0.05);the intercellular adhesion ability of homogeneous cells increased (P<0.05);the intercellular adhesion ability of heterogenous cells decreased (P<0.05).The expression of EMP1 protein in transfection group was obviously higher,VEGFC ,MMP2 protein significangtly lower (P<0.05).Conclusion: miR-95 may promote the invasion and migration ability of SW620 cells,its may be achieved by EMP1,VEGFC,MMP2.
Key words: Key words : mir-95 , colon cancer, invasive, migration, EMP1
CLC Number:
R735.3
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http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2015/V35/I12/1633