Table of Content

05 January 2013, Volume 33 Issue 1

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The Research progressions of Latest Molecular Cell Biology Method and Technology

2013, 33(1):  1-2. 

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The technology and application progressions of induced pluripotent stem cells

2013, 33(1):  3-8. 

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This article reviewed the technological progression, clinical application and existing problems of the induced pluripotent stem cells

microRNA, cell cycle regulation, and its research method

2013, 33(1):  9-14. 

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Recent studies have identi?ed speci?c microRNAs that regulate classic cell-cycle control pathways by directly targeting proteins such as E2F transcription factors, cyclin-dependent kinases (Cdks), cyclins and Cdk inhibitors, and have documented that the loss or gain of miRNA-mediated cell-cycle control contributes to malignancy. This review summarizes experimental strategies for miRNAs research and recent progression on the involvement of miRNAs in cell cycle regulation and tumorigenesis.

Loop-mediated isothermal amplification technology and its application in pathogenic microorganism detection

2013, 33(1):  19-13. 

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Loop-mediated isothermal amplification (LAMP) technology is a novel rapid nucleic acid isothermal amplification technology developed in recent years. Because of its high effiency, rapidity, specificity, and simplicity, scientists believe it will be a common amplification technology replacing the conventional PCR method. This paper briefly reviews the principle of the technology, techinical characteristics, progress, and its application in pathogenic microorganism detection.

P450 Oxidoreductase Deficiency: Clinical Manifestations and Prenatal Diagnosis by Amniocentesis

2013, 33(1):  24-27. 

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Objective To investigate the values of P450 oxidoreductase (POR) gene test by amniocentesis for prenatal diagnosis in a pregnant woman whose husband had a heterozygous mutation in POR gene. Methods In this case report, the abnormal pregnant history in mother and genitalia deformities in her fetus were described. POR gene from the woman and her husband was tested by PCR. During her second pregnancy, the cells from amniotic fluid were collected for POR gene test. The external genitalia and bone status was evaluated after the baby was born. Results 1) the woman presented excessive virilization, such as deepen voice and facial acnes, during her first pregnancy. Her baby twins, manifesting elbow osseous fusion, clitoridauxe and cerebral palsy, died 2 weeks after birth. 2) A heterozygous mutation in POR gene (G1370A, Arg457His) was revealed in the peripheral blood cells from her husband. 3) During her second pregnancy, amniocentesis was conducted and POR gene test for cells from amniotic fluid was negative. 4) A female baby was born uneventfully with normal external genitalia and bone status. Conclusions POR gene test in amniotic fluid can provide important information for prenatal diagnosis.

DDAH2 overexpression inhibits hypoxia-induced apoptosis of rat cardiomyocytes

2013, 33(1):  28-32. 

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Objective To investigate the inhibitory effect of DDAH2 on the apoptosis induced by hypoxia in rat cardiomyocytes through phosphorylation of eNOS. Methods Cell apoptosis was induce by hypoxia within 36hour and was determined by wst-1 and flow cytometry. The phosphorylation of eNOS was examined by western-blot. Results Survival rate of hypoxia group was significantly decreased compared with control group; overexpression DDAH2 induces survival of myocardial cells markedly. After transfection of DDAH2 gene to cardiomyocytes, hypoxia-induced apoptosis significantly declined (17.38%±1.52% vs 27.34%±1.33%, P<0.05). DDAH2 gene transfection significantly increased level of phosphorylation of eNOS in hypoxia of myocardial cells. eNOS inhibitor, L-NAME alleviated the anti-apoptosis effect of overexpression-DDAH2 significantly in H9c2(2-1).Conclusion Overexpression DDAH2 inhibits the apoptosis effects of hypoxia on rat cardiomyocytes H9c2 (2-1) by upregulation of phosphorylation of eNOS.

Human mesenchymal stem cell transplantation promotes functional recovery of macaca fascicularis with cerebral ischemia

2013, 33(1):  33-38. 

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Objective By using human bone marrow-derived mesenchymal stem cell (hBMSC) transplantation to treat experimentally induced cerebral ischemia in Macaca fascicularis to examine the effects of hBMSC transplantation on the recovery of neurological function. Methods In 18 Macaca fasciculari, cerebral ischemia was induced by photochemical occlusion to generate the stoke model. The animals were randomly divided into high-dose, low-dose, and control groups. After 4 weeks, 250 μl of hBMSC at 5×106, 1×106 , or saline were injected respectively at the edge of the hematoma. High field MR scanning and positron-emission tomography (PET) scans were performed every 2 weeks before and after the hBMSC transplant. Neurological functioning and upper limb motor test were used to evaluate on 24hrs,3d,and 1, 2, 3, 4,5,6,7,8,9,10,12weeks after the surgery. Results After hBMSC transplantation, neural function scores and upper limb motor test improved significantly in the high-and low-dose groups compared with the control group. 4and 6 weeks after the transplantation, SUV% of the surrounding area of the lesion had significant different between dose group and control group(P<0.05). At week 4 and 12, pathological examination showed a large amount of angiogenesis around the lesion. The area of the lesion of low-dose group decreased significant compared to the control group (P <0.05). Conclusions- Intracerebral transplantation of human mesenchymal stem cells can improve functional recovery of Macaca fascicularis and can be used to restore neurological deficits in experimentally induced ischemia stroke.

NF-кB participate in delayed protective of sevoflurane preconditioning on myocardium against ischemia-reperfusion injury in rats

2013, 33(1):  39-43. 

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Objective To investigate the delayed protective of sevoflurane preconditioning (SWOP) against myocardium ischemia/reperfusion (I/R) injury and the mechanism of NF-кB during SWOP. Methods Adult male Sprague-Dawley rats were randomly assigned to receive 33% oxygen (I/R group) with or without 2.5% sevoflurane for 2 h (SWOP group). The NF-κB inhibitor parthenolide (500 μg/kg) was administered intraperitoneally before sevoflurane exposure (PTN+SWOP group). Infarct size was measured by TTC staining. The related proteins of NF-κB were determined with Western blot. Results Sevoflurane significantly reduced infarct size, and this effect was blocked by PTN. Sevoflurane up-regulated the expression of NF-кB (p65, p50) proteins compared with CON group before ischemia. The expression of NF-кB (p65, p50) proteins were up-regulated in I/R and SWOP group compared with CON group after reperfusion, but the they were lower in SWOP group. Conclusion Preconditioning with sevoflurane reduces myocardial infarct size, produces SWOP. NF-κB may play an important role in the mechanism of SWOP against myocardium I/R injury.

Oleic acid increase the expression of TLR4 and inflammatory cytokines mRNA and proteins in HA-VSMC

2013, 33(1):  44-48. 

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Objective To investigate the regulation of TLR4 and inflammatory cytokines by oleic acid in human aortic vascular smooth muscle cells (HA-VSMC). Methods MMT methods were used to analyze the proliferation ability of human aortic vascular smooth muscle cells. TLR4, IL-6, IL-8 and MCP-1mRNA expressions were detected by quantitative real-time PCR. TLR4, IL-6, IL-8 and MCP-1 protein levels were measured by western blot and ELISA. Results Oleic acid induced the proliferation ability of HA-VSMC, but the proliferation ability of cells were inhibited with the rising of dose. Oleic acid upregulated the expression of TLR4, IL-6, IL-8 and MCP-1 mRNA by 6.51 fold,7.57 fold,7.62 fold and 7.21 fold, and the levels of IL-6, IL-8 and MCP-1 proteins were significantly enhanced by 2.06 fold,1.97 fold and 1.96 fold (all P<0.01). The levels of TLR4 protein (1.70±0.62) were markedly augmented by 200μmol/L oleic acid than that of control(0.29±0.22). LPS markedly increased TLR4, IL-6, IL-8 and MCP-1 expression in HA-VSMC (P<0.01). Conclusion Oleic acid induced the expression of TLR4 and inflammatory cytokines mRNA and proteins in HA-VSMC.

Calcitonin gene-related peptide alleviates typeⅡ alveolar epithelial cells injury induced by hyperoxia and influences Gli1 expression

2013, 33(1):  49-54. 

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Objective To explore the protection of calcitonin gene-related peptide(CGRP) on typeⅡ alveolar epithelial cells(AECⅡ) injuries induced by 95% oxygen, and influence on Gli1 protein in Sonic hedgehog(Shh) signal pathway. Methods AECⅡs were isolated and purified from premature rats and exposed to air, air+CGRP, air+CGRP+CGRP antagonists(CGRP8-37), hyperoxia(95% oxygen), hyperoxia+CGRP and hyperoxia+CGRP+CGRP8-37 for 24 h respectively. Morphologic change of AECⅡs were observed under electron microscope. The apoptosis ratio of AECⅡs were detected by flow cytometry. The level of cellular reactive oxygen species (ROS) were measured by fluorescence molecular probes, malondialdehyde (MDA) and superoxide dismutase(SOD) in AECⅡs were determined by spectrophotography. The expression of surfactant protein C (SPC) mRNA and protein of Gli11 in AECⅡs were also detected by real-time quantitative polymerase chain reaction(RT-qPCR) or western blot. Results Under the 95% oxygen condition, the apoptosis ratio of AECⅡs were increased 3.6-fold, the level of ROS increased 1.5-fold , and the activation of MDA and SOD also up-regulated sharply, SPC mRNA and Gli11 protein were decreased significantly comparison with air group. Administration of CGRP before hyperoxia could attenuate these changes induced by 95% hyperoxia. Conclusion CGRP can alleviate AECII injuries induced by 95% oxygen, which might be associated with the activation of Gli1 in Shh signal pathway.

5-Aza-2’-deoxycytidine reverses the expression of HepaCAM mRNA and inhibits the growth of T24 and BIU-87Cell

2013, 33(1):  55-59. 

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Objective To investigate the effect of 5-aza-CdR on expression of hepaCAM mRNA and the inhibition of T24 and BIU-87cell lines. Methods The effects of 5-aza-CdR on cell proliferation of T24 and BIU-87 cell lines were measured by MTT; Flow cytometry was used to examine apoptosis of T24 and BIU-87 cells; RT-PCR detected the expression of hepaCAM in T24 and BIU-87 cell lines with or without 5-aza-CdR treatment. Results MTT showed that the inhibition ratios of 3.0 and 10.0 μmol/L 5-aza-CdR were significantly higher than that of 0.3 and 1.0 μmol/L 5-aza-CdR in T24 cell (p<0.05); And the inhibition ratio of 5.0 μmol/L 5-aza-CdR was higher than that of 0.1 and 0.5 μmol/L 5-aza-CdR in BIU-87 cell (p<0.05). Cell apoptotic rate was increased in cells with 5-aza-CdR treatment (p<0.05). The expression of hepaCAM mRNA was reversed in T24 and BIU-87 cells after treatment. Conclosion 5-aza-CdR can reverse the expression of hepaCAM mRNA and inhibit the cell growth through inducing apoptosis in bladder cancer.

Overexpressing GATA-4 increase rat bone marrow mesenchymal stem cells transdifferentiating into cardiomyocytes

2013, 33(1):  60-65. 

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Objective To investigate whether overexpression of GATA-4 increases adult mesenchymal stromal cell (MSC) transdifferentiation into a cardiac phenotype in vitro. Methods MSCs and cardiomyocytes (CMs) were isolated and cultured. MSCs were transduced with GATA-4 (MSCGATA-4) at the third passage. Gene expression in MSCGATA-4 was analyzed using real-time quantitative polymerase chain reaction (real-time PCR) and Western blot and immunohistochemistry. After co-cultured with CM for one week, the expression of cardiac genes of MSCGATA-4, including brain natriuretic peptide (BNP), Islet-1 and α -sarcomeric actinin, was determined by real-time PCR and Western blot. After mixed coculture with CM for one week, the beating of MSC was observed. MSC were examined α-actinin by for immunohistochemistry and the transdifferentiation rate of MSC was calculated directly from ?ow cytometery. Results Compared with control cells that were transfected with Green Fluorescent Protein (GFP) only (MSCNull), MSCGATA-4 were shown by immuno?uorescence, real-time PCR, and Western blot to have higher expression of GATA-4. After cocultured for one week, the mRNA and protein expression of cardiac genes, including BNP, Islet-1 and α -sarcomeric actinin, was up-regulated in MSCGATA-4 compared with MSCNull (P<0.05). After mixed coculture for one week, some MSCs beated and some MSCs were positive for α-actinin staining. The transdifferentiation rate was signi?cantly higher in MSCGATA-4 than MSCNul (P<0.05). Conclusions Overexpression of GATA-4 signi?cantly increases MSC differentiation into a myocardial phenotype and expressing BNP, Islet-1 and α-actinin.

The Change in Hepatic Insulin Sensitivity and Inflammation Factors In Fatty Liver Induced by Short-term High Fat Feeding

2013, 33(1):  66-69. 

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Objective To investigate the effect of short-term (3 days) high fat feeding on liver triglyceride content, hepatic insulin sensitivity and hepatic inflammation pathways. Methods Male C57BL/J6 mice(~30g) were fed a standard chow (Con) or high fat diet(HF). Mice were sacrificed after 3-day feeding. Fasting blood glucose, serum triglyceride and serum alanine transaminase (ALT) were measured. Triglyceride content in liver were measured with GPO method. Western blot method were applied to detect the protein contents of phosphorylated-Akt/total-Akt, phosphorylated -GSK-3α/β/total- GSK-3α/β, phosphorylated-JNK/ total-JNK in JNK pathway, phosphorylated -IKKα/β/ total-IKKα/β and NF-κB in IKKα/β-NF-κB pathway. Results After 3-day feeding, fasting blood glucose, serum TG and serum AST were unaltered in high fat group compared with control group（p>0.05）. Liver triglyceride content was significantly increased in high fat group than in control group (p all﹤0.01), which were 11.03±0.29 and 24.92±2.98（mmol/g） respectively(p﹤0.01). The ratio of phosphorylated-Akt/total-Akt and phosphorylated - GSK-3α/β/ total- GSK-3α/βwere significantly decreased in HF group than in control group after insulin stimulation (p both﹤0.01). The protein expression of p-JNK/t-JNK was significantly increased in high-fat group than in control group (P ﹤0.01). Conclusions JNK pathway in liver is involved in the early development of fatty liver and hepatic insulin resistance induced by high-fat-feeding.

THE EXPRESSIONS OF 4.1 PROTEIN FAMILY MEMBER MERLIN AND EZRIN IN GASTRIC CANCER OF QINGHAI TIBETAN PATIENTS AND THE INFLUENCES ON its CELLULAR FUNCTIONS OF GASTRIC CANCER CELL

2013, 33(1):  70-76. 

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Objective To study the mutation of tumor suppressor protein merlin and the expression of ezrin in gastric cancer of Qinghai Tibetan patients, meanwhile to explore the influences of merlin and ezrin on the proliferation and adhesion ability of gastric cancer cell. Method PCR and DNA sequencing was used to analyze the mutation, and the ezrin expression was detected by immunochemical staining. The proliferation and adhesion ability of gastric cancer cell were analyzed by MTT assay. Results The mutation had been detected in merlin gene NF2 exons, and two sites in exon 17 showed high mutation rate, it was more than 50%. Ezrin protein expression was higher in gastric cancer than normal gastric mucosa in Qinghai Tibetan patients（P<0.05）. The expression level of ezrin protein was correlated with the degree of tumor differentiation, lymph node metastasis and histological type of Qinghai Tibetan patients（P<0.05）. Furthermore, the MTT assay showed that FERM domain and merlin-2 had no significant influences on the proliferation and adhesion ability of gastric cancer cell, and ezrin showed no effect on the adhesion ability of gastric cancer cell, but it showed ezrin influenced the cell proliferation. Tumor suppressor protein merlin-1 showed not only a much stronger inhibition of proliferation, but also decreased the cell adhesion ability of gastric cancer cell, and the inhibition of proliferation by merlin was much stronger than ezrin protein. Conclusion The expression of merlin and ezrin is related with the susceptibility to gastric cancer development and metastasis in Qinghai Tibetan patients. Furthermore, merlin-1 and ezrin are involved with adjustment of proliferation and adhesion ability of gastric cancer cell.

The human testis TRIM69 protein possesses an activity of catalyzing formation of muti-ubiquitination

2013, 33(1):  77-81. 

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Objective To identify whether human TRIM69 could catalyze formation of multiubiquitinylated products. Methods The gene of human TRIM69 was cloned into a procaryotic expression vector, which was then transformed into E.coli. Purified protein of TRIM69 was obtained through the process of induction, expression, and purification, which was used in the following experiment of ‘in vitro ubiquitination assay’ to detect whether TRIM69 could catalyze formation of multiubiquitinylated products. In addition, we constructed TRIM69 gene full length or a point mutagenesis of RING domain sequence or a deleted RING domain sequence into a eukaryotic expression vector. Then the corresponding vectors were transiently transfected into HEK293T cells or HeLa cells of stably expressed ubiquitination. The experiment of immunoprecipitation combined with immunoblotting was performed to detect whether TRIM69 could catalyze formation of multiubiquitinylated products in cells. Results The ubiquitination assay results demonstrated that TRIM69 could catalyze formation of multiubiquitinylated products, which was dependent on its RING domain. Conclusion The enzyme activity of TRIM69 in catalyzing the formation of muti-uiquitinylated products was successfully identified, which builds up a basis for further characterizing TRIM69 as a novel E3 ubiquitin ligase.

Effects of PKC/NADPH oxidase on AGEs-induced eNOS uncoupling in Rat cardiac microvascular endothelial cells

2013, 33(1):  82-87. 

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Objective To observe the Effects of PKC/NADPH oxidase on AGEs-induced eNOS uncoupling in rat cardiac microvascular endothelial cells Methods Cultured the cardiac microvascular endothelial cells in vitro. AGEs (100mg/l) 、 DPI and LY33531 （2.5μmol/L,5μmol/L and 10μmol/L ）respectively, was added and resumed incubation for 24 hrs. Meanwhile, a group managed with serum-free culture solution was assumed as blank control, we determined the BH4 、NO、O2-generation、 eNOS 、 ROS and p47phox protein expression of all groups. Results As LY33531 and DPI concentration was increased, production of NO was noted increased gradually(（90.67±0.33～122.58±0.26、160.58±0.58， P＜0.05) ,while production of O2- was decreased gradually(179.59±0.37～125.59±0.57、125.51±0.65，P＜0.05)，BH4 expression was seen increased (213.73±0.01～434.55±0.01、480.89±0.01，P＜0.05)， eNOS expression was seen gradually decreased (126.10±3.49～112.61±1.76、114.43±1.75，P＜0.05) .Meanwhile, p47phox protein expression and ROS was seen decreased gradually (P＜0.05). Conclusion AGEs may trigger the activation of PKC/NADPH oxidative stress pathway inducing intracellular eNOS uncoupling in cardiac microvascular endothelial cells.

Effect of silencing hypoxia inducible factor 1α by siRNA on the expression of cardiotrophin-1 in rat myocardium cell

2013, 33(1):  88-92. 

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Objective To study the regulation mechanism of hypoxia-inducible factor-1α（HIF-1α）in rat myocardium cell under hypoxia environment. Methods Small interference RNA （siRNA） targeing HIF-1α was constructed and transfected into rat myocardium cell lines(H9C2).The HIF-1α and cardiotrophin-1（CT-1 ）gene expression at mRNA levels were detected by real-time PCR. The HIF-1α and CT-1 gene expression at protein levels were detected by western blot. Results Expression of the mRNA and protein level of HIF-1α and CT-1 were down regulated after silencing of HIF-1α under hypoxia environment(P<0.05). Expression of the protein level of HIF-1α and the mRNA and protein level of CT-1 increased after exposure of untransfected H9C2 cells to hypoxia(P<0.05). Expression of the protein level of CT-1 increased from 0.34±0.05 to 0.55±0.05(P<0.05).Conclusion The regulation of CT-1 gene under hypoxia could be activated by HIF-1α that plays an important role in myocardial adaptation to hypoxia.

The expression and clinical significance of LATS2 in epithelial ovarian cancer

2013, 33(1):  93-97. 

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Objective To detect the expression of the large tumor suppressor homolog 2(LATS2) in human epithelial ovarian cancer tissue and to analysis the correlation with the tumor progression. Methods RT-PCR、SYBR Green quantitative real-time PCR and Immunohistochemistry were employed to detect the expression the of LATS2 mRNA and protein. Results The expression of LATS2 mRNA in epithelial ovarian cancer was significant lower than that in benign，and normal group(P<0．05)，and there are obvious connection between stage and metastasis of lymph node (P<0．05)．The LATS2 was mainly located in cytolymph ．The expression of LATS2 protein in epithelial ovarian cancer group was significant lower than that in the other groups(P<0．05)．There are same results between the different stage and metastasis of lymph node. Conclusion LATS2 might play a role in the tumorigenesis of epithelial Ovarian cancer. It maybe has certain progression for early diagnosis and forecast of ovarian cancer.

Tiotropium inhibition of the proliferation of airway smooth muscle cells stimulated by leptin in rats

2013, 33(1):  98-101. 

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Objective To explore the effects of tiotropium on the proliferation of rats’ airway smooth muscle cells by leptin stimulating. Methods ASMCs were derived from rat airway tissue and cultured in vitro. Then ASMCs were treated with leptin (the final concentration 100 μg/L) and tiotropium at different concerntrations (μg/L) (15、30、60 and 120) and cell proliferation was measured by CCK-8 assay. Realtime RT-PCR and Western blot were used to verify the expressions of leptin receptors on ASMCs. Results Leptin could promote the proliferation of ASMCs compared with control group (P<0.05). After treated with tiotropium, leptin’s promotion of the proliferation of ASMCs was inhibited in a dose-dependent manner (P<0.01). After treated with leptin, the expressions of leptin receptor mRNA were increased and the proteins were rose from 0.327±0.011 to 0.541±0.016 （P<0.05）; then treated with different concerntrations of tiotropium, the expressions of leptin receptor mRNA were decreased and the proteins were reduced respectively to 0.514±0.017、0.468±0.025、0.331±0.023 and 0.313±0.017（P<0.05）. The expressions of leptin receptors were positively related to tiotropium concentrations (P<0.01). Conclusion Tiotropium can inhibit the proliferation of rats’ airway smooth muscle cells stimulated by leptin, and its mechanism may be correlated to the decreased the expression of leptin receptors on rats’ ASMCs.

Recombinant human granulocyte colony stimulating factor accelerates wound healing after treatment of chemotherapy and surgery

2013, 33(1):  102-105. 

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Objective To study the effect of rh-GCSF on wound healing after treatment of chemotherapy and surgery in rabbits ,and the effect of VEGF and TNF-α. Methods New Zealand white rabbits (24cases),were randomly divided into 3 groups: normal group(8cases) was given surgery and medication, control group(8cases) and treatment group(8cases) were given infusion of epirubicin on the day before the operation，control group was treated as routine and treatment group was given rh-GCSF. Results The differences were observed including wound healing, VEGF and TNF-α in 7th day after surgery. The VEGF in treatment group was increased and the wound healing was better than the control group, but there was no difference of TNF-α in treatment and control group. Conclusion The rh-GCSF can increase the VEGF expression of incision sites, and it is helpful to wound healing after treatment with both chemotherapy and surgery.

Influence of lysophosphatidic acid on proliferation of breast cancer cell by adjusted RhoA/ROCK2 signal pathway

2013, 33(1):  106-110. 

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Objective To investigate the influence and mechanism of lysophosphatidic acid and RhoA/ROCK2 signal pathway on proliferation of breast cancer cell. Methods After breast cancer cell(MDA-MB-231) was treated with different concentration of LPA, the proliferation of MDA-MB-231 was observed and recorded by cell count method every of 24h. MDA-MB-231 was treated with optimal concentration of LPA and observed the effect of Rho kinase inhibitor(Y-27632) on LPA-induced proliferation. The activity of RhoA was tested by Pull-down way. The protein expression of RhoA and ROCK2 were determined by Western blot. Results LPA could promote MDA-MB-231 proliferation in a time and dose-dependent manner (P<0.05). ROCK inhibitor could significantly inhibit LPA-induced cell proliferation(P<0.05). The activity of RhoA and expressionof RhoA, ROCK2 were enhanced significantly affected by LPA (P<0.05). However Y-27632 markedly decreased LPA-induced the increase of RhoA activity and protein expression of RhoA and ROCK2 (P<0.05). Conclusions LPA may promote breast cancer cell proliferation through regulating by RhoA/ROCK2 signal pathway. It provides a new idea for breast cancer clinical treatment.

High fever as the predominant presenting symptom of collapsed multiple myeloma：A case report

Chan Meng Wei LV

2013, 33(1):  111-114. 

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Objective：Improve understanding and management of non-infectious fever in multiple myeloma patients. Methods: Study the differentiation and treatment of a collapsed multiple myeloma case predominantly presented as a high fever，and review the correlative literatures. Results：A 56 year-old male with diagnosed multiple myeloma for 5 years got a high fever when his disease collapsed and widely extra-medullary infiltrated. Examinations for pathogens were negative and antibiotics treatment had no effect. Though the temperature became normal after chemotherapy，the patient died of complication. Conclusion: For myeloma patients，non-infectious fever may be either the admission reason or the presentation as the disease deteriorates. If there is no evidence for infection，chemotherapy should be administrated early.

Analysis of KRAS mutation in non-small cell carcinoma of lung and colorectal cancer

2013, 33(1):  115-116. 

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Dexmedetomidine abate cerebral ischemic-reperfusion injury in ischemia-reperfusion rats

2013, 33(1):  117-118. 

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Transgenic Animal Models of Polycystic Ovary Syndrome

2013, 33(1):  119-122. 

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Polycystic ovary syndrome is the most common endocrinal and metabolic disease in reproductive female. The etiology is still unclear. Building an appropriate animal model will improve the knowledge of etiology, pathogenesis, clinical strategies and the prevention for complication of PCOS. The previous animal models are induced by estrogen, androgen or constant illumination. Recently, new genetically modified animal model, such as transgenic, generalized knock-out or tissue specific knock-out mice were applied in the research of PCOS. The present review summarizes the origin, features, research progression, application and advantages/disadvantages of several PCOS-like mice, providing optimal model for research.

The progress of studies on the role of EIF5A2 in human cancer

2013, 33(1):  123-125. 

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Eukaryotic initiation factor 5A2 gene is a highly conserved gene of EIF5A family. EIF5A2, as a potential oncogene, was highly expressed in a variety of human tumors, and associated with the staging and / or poor prognosis of the tumor. EIF5A2 induces epithelial mesenchymal transition through a variety of the mechanisms, and thus promote tumor invasion and metastasis. EIF5A-MTA1/c-MYC axis may be one of the important pathways involved in regulation of tumor invasion and metastasis. The underlying molecular mechanisms need further studies.

Setting up and application of a comparative morphology experiment education system with case guiding

2013, 33(1):  126-128. 

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A comparative morphology experiment education system with case guiding based on integrated morphology experiment subject was introduced. The learning efficiency of medical students increases by combining the normal structure and pathological changes of human body and compared learning the knowledge of anatomy, histology and pathology.