Abstract: Objective To investigate the effect of short-term (3 days) high fat feeding on liver triglyceride content, hepatic insulin sensitivity and hepatic inflammation pathways. Methods Male C57BL/J6 mice(~30g) were fed a standard chow (Con) or high fat diet(HF). Mice were sacrificed after 3-day feeding. Fasting blood glucose, serum triglyceride and serum alanine transaminase (ALT) were measured. Triglyceride content in liver were measured with GPO method. Western blot method were applied to detect the protein contents of phosphorylated-Akt/total-Akt, phosphorylated -GSK-3α/β/total- GSK-3α/β, phosphorylated-JNK/ total-JNK in JNK pathway, phosphorylated -IKKα/β/ total-IKKα/β and NF-κB in IKKα/β-NF-κB pathway. Results After 3-day feeding, fasting blood glucose, serum TG and serum AST were unaltered in high fat group compared with control group（p>0.05）. Liver triglyceride content was significantly increased in high fat group than in control group (p all﹤0.01), which were 11.03±0.29 and 24.92±2.98（mmol/g） respectively(p﹤0.01). The ratio of phosphorylated-Akt/total-Akt and phosphorylated - GSK-3α/β/ total- GSK-3α/βwere significantly decreased in HF group than in control group after insulin stimulation (p both﹤0.01). The protein expression of p-JNK/t-JNK was significantly increased in high-fat group than in control group (P ﹤0.01). Conclusions JNK pathway in liver is involved in the early development of fatty liver and hepatic insulin resistance induced by high-fat-feeding.

Key words: triglyceride, fatty liver, insulin resistance, inflammation