Abstract: Objective To investigate the effect of 5-aza-CdR on expression of hepaCAM mRNA and the inhibition of T24 and BIU-87cell lines. Methods The effects of 5-aza-CdR on cell proliferation of T24 and BIU-87 cell lines were measured by MTT; Flow cytometry was used to examine apoptosis of T24 and BIU-87 cells; RT-PCR detected the expression of hepaCAM in T24 and BIU-87 cell lines with or without 5-aza-CdR treatment. Results MTT showed that the inhibition ratios of 3.0 and 10.0 μmol/L 5-aza-CdR were significantly higher than that of 0.3 and 1.0 μmol/L 5-aza-CdR in T24 cell (p<0.05); And the inhibition ratio of 5.0 μmol/L 5-aza-CdR was higher than that of 0.1 and 0.5 μmol/L 5-aza-CdR in BIU-87 cell (p<0.05). Cell apoptotic rate was increased in cells with 5-aza-CdR treatment (p<0.05). The expression of hepaCAM mRNA was reversed in T24 and BIU-87 cells after treatment. Conclosion 5-aza-CdR can reverse the expression of hepaCAM mRNA and inhibit the cell growth through inducing apoptosis in bladder cancer.

Key words: 5-aza-CdR, hepaCAM, bladder cancer, apoptosis