Table of Content

05 September 2010, Volume 30 Issue 9

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研究论文

Preparation and biocompatibility evaluation of polyurethane filled with carbon nanotubes

Jie MENG; Xiao-tian GUO; Hua KONG; Man YANG; Zhen XU; Hai-yan XU

2010, 30(9):  897-901. 

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Objective To prepare and evaluate the biocompatibility of carbon nanotubes-polyurethane composite. Method multiwalled carbon nanotubes were introduced into a polyurethane elastomer through a lab developed process of sol-gel. The biocompatibilities including cytotoxcicity, tissue compatibility, hemolysis, dynamic coagulation time, and platelets activation have been evaluated according to the guideline of ISO10993. The tensile strength and elongation rate of the composite were also tested. Result Experimental results indicated that the composite remained the excellent mechanical property of polyurethane; meanwhile, there was no cytotoxicity observed with fibroblasts, inflammation was obvious lighter, and platelets activation was significantly decreased. Conclusion A new composite of carbon nanotube-polyurethane were obtained and its biocompatibility was improved notably.

Expression, Purification and Identification of CPPs-EGFP Fusion Protein

Jie ZHOU; Xiao-yun GUO; Jing LENG; Yan-bin WAN

2010, 30(9):  902-906. 

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Objective To construct EGFP-CPPs fusion genetic carrier,express,purify and identify EGFP-CPPs fusion protein.Methods Upstream primer containing CPPs gene sequence of green fluorescent was synthesized by basic radical synthesise methods.CPPs-EGFP fusion gene fragment was amplificatied by PCR methods and was indentified by electrophoresis. Bacillus coli were transfected with the fusion gene express vector and the fusion gene was amplificated and expressed.Fusion protein was collected and purified by column chromatography.Protein molecular mass was detected by protein electrophoresis.Protein sequence was detected by TOF analysis.Cell-penetrating function was tested by vitro cell experiment. Results Electrophoresis site corresponding to EGFP-CPPs was displayed in electrophoresis;The gene sequence of constructed plasmid contained interest gene sequence.Purified fusion protein was displayed in protein electrophoresis.Protein sequence was in accord with interested protein sequence. CPPs-EGFP fusion protein displayed Cell-penetrating function in vitro. Conclusions CPPs-EGFP fusion protein with cell-penetrating function was prepared successfully in the experiment.It will establish basis for study of promoting antibody-albumin nanosphere conjugate to enter tumor cell and bring into full play therapeutical effect.

Preparation and identification of monoclonal antibodies to YTMLC-90 cells

Rui-zhu TANG; Ji-mei LI; Li BIAN; Li-ju MA; Ping HAO; Qin-qin WANG

2010, 30(9):  907-911. 

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Objective To obtain hybridoma cell lines which can secrete high-titer monoclonal antibodies against human lung squamous cell carcinoma YTMLC-90 cell line. Methods Balb/c mice were immunized by a human lung squamous cell carcinoma YTMLC-90 cell line. And the immunized splenocytes were fused separately with two murine myeloma cell lines SP2/0 and NS-1 using hybridoma technique. And then, positive hybridomas were selected to cloned culture and subcloned by limited dilution. The titers of McAbs from cell culture supernatant and ascites were assayed by ELISA. The isotype of the McAbs were also identified by ELISA and the average number of chromosome of the hybridoma cell lines were counted by a general method after stained with Gimesa. McAb's cross-reaction with different cells and tissues were detected by ABC immunohistochemistry technique. Results Stable hybridomas S2D1, N2D1, N3C5 were constructed. The titers of these 3 McAbs from cell culture supernatant and ascites were 1:512,1:284,1:1024 and 1:105,1:104,1:104 respectively. The isotypes were IgG1, IgG2a and IgG3. The numbers of chromosome were 90~110. No cross-reaction with other cell lines and tissues. Conclusion Specific and high titer antibodies against human lung cancer were successfully prepared.

Cardiac Repolarization Analysis Based on T-wave Morphology in an Adult Population from Heilongjiang Province

Xiao-lin YANG; Jing ZHANG; Shao-mei HAN; Zheng-guo ZHANG; Guang-jin ZHU

2010, 30(9):  912-917. 

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Objective To analyze the characteristics of ventricular repolarization variables based on T-wave morphology and to determine their relationships with age, sex, blood pressure, heart rate and cardiac hypertrophy in an adult population from Heilongjiang province. Methods A total of 2514 subjects (range 35-81years; 43% men) were included. Bazett corrected QT interval (QTcB), Fridericia corrected QT interval (QTcF) and four T-wave morphology parameters total cosine R-to-T(TCRT), T-wave morphology dispersion(TMD), relative T-wave residuum(rTWR) and principle component T-wave analysis ratio(PCA ratio) were used to evaluate the ventricular repolarization. Multivariate regression was used to test for the dependence of T-wave morphology variables with the subject age, sex, systolic blood pressure and Sokolow index. Result All ventricular repolarization variables had significant sex different (P<0.05). All parameters but TCRT were higher in the high blood pressure group (P<0.05). Age, sex and systolic pressure had independent effect on ventricular repolarization variables. Sokolow index only had effect on T-wave morphology variables. Conclusion T-wave morphology variables are quantified marker of ventricular repolarization heterogeneity, which was influenced by age, sex, blood pressure and myocardial hypertrophy.

The protective effect of rAAV-BF on different strains of mice from lethal bacteria infection

Xiang-yu GUAN; Zhen-long LIU; Zhe LV; Qing-li KONG; Wei WANG; Yun-qing AN

2010, 30(9):  918-924. 

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Objective The purpose of this study is to observe the protective effect of rAAV-BF infected mice against lethal dose of E.coli.Methods The rBF protein in CHO cells、mice injected muscle and serum were detected by Dot-blot、Western blot、immunohistochemistry assay and a modified enzyme linked immunosorbent assay(ELISA),the levels of endotoxin were measured by reagent kits,establishing MLD E.coli causing death model for the detection of the mice resistant to MLD E.coli infection after administration with rAAV-BF.Results (1)The presence of rBF protein was detected in CHO cells and injected mice muscle, serum from the gene transfected mice could neutralize endotoxin and kills E.coli.(2)The survival rate of Balb/c and C57BL/6 mice of rAAV group（30%-40%）was significantly higher than control group（0%）.Serum endotoxin level of rAAV-BF group mice was significantly lower than that control group. rAAV-BF plus antibiotics have synergistic effect：the survival rate of rAAV-BF group was significantly higher than that of control group; the survival rate（85%） of rAAV-BF infected C57BL/6 mice was significantly higher than that of Balb/c mice（65%）.Conclusion rAAV-BF infected mice have restistence to lethal dose of E.coli, which might provide the base to protect clinical high risk patients from being infected by bacteria and accordingly to reduce mortality.

Effect of Tectoridin on Serum Lipid in Rat Model with hyperlipidemia

Jin-feng WANG; Cui-yan YANG; Yan-ping ZHANG; Ming-xiao HOU; Fang WANG; Guo-yu WANG; Shu-hong WANG

2010, 30(9):  925-929. 

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Objective To investigate the effect of Tectoridin on the lipoprotein of the rat with hyperlipidemia and it?s mechanism. Method 48 healthy male Wistar rats were devided randomly into four groups, high fat diet model group; high doge Tectoridin group; low doge Tectoridin group and Atorvastin group. Each rat was given drugs with intragastric administration after fed with high fat forage for 2 weeks. All the rats blood lipid was mensurated after 12 weeks. LDL-R and it?s mRNA expression in rats? liver were determined by immunohistochemical and RT-PCR method. Result Tectoridin markedly reduced the rat serum lipid and raised the LDL-R and it?s mRNA expression in rats? liver, especially to total cholesterol and low density lipoprotein-cholesterol. Conclusion Tectoridin had significant effect on reducing the rat?s serum lipid perhaps because of increasing the expression of LDL-R in liver of the rat with hyperlipidemia.

Transmyocardial Drilling Revascularization Combined with Heparinized bFGF-Incorporating Stent Promotes Regeneration of Myocardium after Pig Acute Myocardial Infarction

Guang-wei ZHANG; Xiao-cheng LIU; Rong-fang SHI; Xiao-bin ZHAO; Tian-jun LIU; Feng LV

2010, 30(9):  930-934. 

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Objective To investigate the effect of transmyocardial drilling revascularization combined with heparinized bFGF-incorporating degradable tubular stent implantation (TMDRSI) on myocardial regeneration after acute myocardial infarction. Methods After the mid third of left anterior descending (LAD) was ligated, miniswine were grouped into control group and treatment group (n=6 each group). In treatment group, two transmural channels with 3.5 mm in diameter were established by using a self-made drilling device, followed by implantation of two stents into the channels. All animals received 5-bromo-2-deoxyuridine (BrdU) 250 mg IV twice a week post-operatively, to label cells undergoing DNA replication. Expression of stromal cell-derived factor 1 (SDF-1) was determined with RT-PCR. Myocardial perfusion and left ventricular ejection fraction (LVEF) were evaluated by 99m Tc-MIBI Myocardial Perfusion Imaging. Histological and morphological analyses were used to observe myocardial regeneration. Results At 6 weeks post-treatment, expression of SDF-1 significantly upregulated in treatment group (4.02±0.31), compared with control group (1.63±0.21, P<0.001). Change of Mass defect percent significantly decreased in treatment group (-3.22%±0.46%), compared with control group (2.83%±0.31%, P<0.001). A value of BrdU in treatment group (1639±105 pixels/hpf) was more than that in control group (240±53 pixels/hpf, P<0.001). A value of area of viable myocardium (94118±4476 pixels/hpf) in treatment group was higher than that in control group (36345±3714 pixels/hpf, P<0.001). LVEF was significantly improved in treatment group (61%±4%), compared with control group (49%±3%, P<0.001). Conclusions TMDRSI may be a new method for the regeneration treatment of acute myocardial infarction.

Rosiglitazone up-regulates expression of CPTⅠand PPARγ in skeletal muscles of aged rat fed high-fat diets

Yu GAO; Hui-juan MA; Jing WANG; Dong-ming QU; Guang-yao SONG; Shu-guo HU

2010, 30(9):  935-939. 

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Objective To observe the expression of carnitine palmitoyltransferase Ⅰ (CPTⅠ) and peroxisome proliferators activated receptorγ(PPARγ) in high-fat-fed aged rats and rosiglitazone intervention. Methods Wistar rats were divided into control group, high-fat diet (HF) group and high-fat diet plus rosiglitazone maleate tablets (RSG) group, (n=20 in each group). Insulin sensitivity was evaluated by conscious hyperinsulinemic-euglycemic clamp techniques, the expression of CPTⅠmRNA and PPARγmRNA and protein were analyzed by RT-PCR and Western-blot. Results Skeletal muscle triglyceride was significantly higher and glucose infusion rate was significantly lower in the control group than those in the high-fat diets group. The expression of CPTⅠ and PPARγ decreased in HF group, RSG group reserves above of all the changes. Conclusion It is suggested that a increased expression of CPTⅠand PPARγ play a pivotal role in insulin-sensitizing effect of rosiglitazone treatment, maybe the one of the mechanism of improving insulin resistance.

Inhibition of TGF-β1 expression by sodium ferulate in rats' myocardium necrosis model

Hai-cheng GAO; Hai-mei GAO; Ying-gang ZOU; Li-qun REN

2010, 30(9):  940-943. 

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Objective To investigate the TGF-β1 expression and the effects of sodium ferulate in rats' myocardium necrosis model. Methods Rat myocardium necrosis model was induced by Isoproterenol (15mg/kg). The rats were injected SF to carry out intervention studies after stable 2 days. TGF-β1 protein and mRNA expression in myocardium were detected by immunohistochemistry and RT-PCR. Results TGF-β1 mRNA expression increased obviously as compared with control group at 24 h（P <0.05）and decreased gradually at 48 h. TGF-β1 protein expression increased to follow the severity. TGF-β1 mRNA expression decreased gradually by injection SF later, its protein expression was also improving. Conclusion SF could inhibit TGF-β1 expression in MF.

Relationship between SnoN , Smad2/3 signaling protein and fibrosis of renal tubule-interstitium in rats with diabetes

Rui-xia LIU; Bing GUO; YIng XIAO; Ming-jun SHI; Yuan-yuan WANG; Xiao-ying LI; Guo-zhong ZHANG

2010, 30(9):  944-951. 

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Objective This study was aimed to explore the expression of transcription co-repressor Ski-related protein N(SnoN) and Smad2/3 in renal tissues of diabetic rats, and to investigate their contribution to the fibrosis of renal tubule-interstitium in diabetic rats. Methods The rat model of diabetes was induced by vena caudalis injection of streptozotocin (STZ). Rats in different groups were killed at 2, 4, 8, 12,16 and 24 week of experiments. Meanwhile, the rats were in age-matched as normal control(n=6). The protein or mRNA levels of SnoN, TGF-β1, Smad2/3, α-SMA and fibronectin( FN) in renal tissues were detected. Blood glucose, serum creatinine and 24h urine protein were examined by biochemistry methods. Results The renal SnoN protein level was significant decrease from DM4 weeks(P < 0.01). However, the SnoN mRNA levels were unchanged in each diabetes group, The protein levels of TGF-β1, Smad2/3 and FN were significantly up-regulated in diabetes rats, in diabetes rats, α-SMA tubules staining was seen from 12th week. Conclusion These results showed that SnoN and TGF-β1/Smad signaling pathway may play a key role in the pathogenesis of diabetic nephropathy.

Establishment of hybridoma cell strains expressing membrane- specific antibody against AchR

Qing ZHAO; Na YIN; Wei ZHANG

2010, 30(9):  952-955. 

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Objective To establish cell strains that express membrane-specific anti-AchR autoantibodies. Methods The extracellular reagion of AchR subunit was expressed and used as an immunogen to immunize mice. Using hybridoma technology to get anti-AchR antibody cell strains. Flow cytometry analysis was performed to detect membrane specific antibody and membrane Ig expression. SDS-PAGE was performed to compare molecular weight of membrane and secreted antibodies. Immunoprecipitation and Western blot were performed to charicterize membrane and secretary antibodies. Results Hybridoma cells could not only secrete antibodies, but also expresse membrane antibodies. Conclusion Membrane anti-AchR antibody expressing hybridoma cells were similar as membrane autoantibody expression B cells in Myasthernia Gravis and may be used as target cells for studying this disease.

Peroxynitrite Increases the Expression of PDCD4 Gene in Human Cerebral Vascular Smooth Muscle Cells

Lin LI; Xiao-rui CHEN; Sha-sha SONG; Yu-lian JIAO; Chun-yan MA; Yuan-rong JU; Jian-feng LI

2010, 30(9):  956-960. 

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Objective This study was designed to investigate the change of PDCD4 gene expression in cerebral vascular smooth muscle cells (HCVSMCs) in response to peroxynitrite (ONOO-). Methods HCVSMCs were cultured in vitro and subjected to different concentrations of ONOO-. Cell viability was measured by MTT assay. The morphological changes were assessed by acridine orange staining as well as Ho33342/PI double staining to determine whether cell experienced apoptosis. The apoptotic rate was determined by flow cytometry. In particular, the expression of PDCD4 gene was examined by the means of RT-PCR and Western blot simultaneously. Results Direct exposure of HCVSMCs to ONOO- was able to block the cell proliferation, which was further revealed via the apoptotic pathway. Under the stress by ONOO-, the expression of PDCD4 gene was apparently increased at both mRNA and protein levels. Conclusions PDCD4 gene may play a pivotal role underlying the ONOO--induced apoptosis in HCVSMCs.

Angiopoietin-1 prevents rat cardiomyocytes from H2O2-induced apoptosis by regulating the activity of PI3K/Akt

Yuan-peng LI; Yi-qing WANG; Peng ZHANG

2010, 30(9):  961-965. 

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Objective To investigated whether Angiopoietin-1 (Ang-1) has direct cytoprotective effects on rat cardiomyocytes against oxidative stress and its possible signaling pathway involved.Methods A model for primary culture of cardiomyocytes injuried by H2O2 was established, the cultured cardiomyocytes were divided randomly into four groups.The morphology of cardiomyocytes was observed by Hoechst-33342, Apoptosis rate of cardiomyocytes was determined by flow cytometry (FCM), the expression of p-akt、active-caspase 3 were measured by western blot analysis.Results The flow cytometry(FCM) analysis showed that the percentage of apoptosis cells in control group and Ang-1 intervention group were 2.13±0.61%、31.20±2.01%，and compared with the induced group（48.16±1.37%）was significantly lower(P<0.01), and apoptosis rate of cointervention group was 47.42±2.02%,has no significant difference with induced group(p＞0.05). Western blotting analysis showed Ang-1 restored the phosphorylation of Akt and inhibited the activation of caspase 3,and these two effects were reversed by the presence of LY294002.Conclusion This study suggest that Angiopoietin-1 (Ang-1) has direct cytoprotective effects on cardiomyocytes against oxidative stress and this effects is dependent on the PI3K/Akt pathway and inhibition of caspase 3 cleavage.

Rb1 Reduce the Damage of Aβ25~35 on Neonatal Rat Neural Cells

Ping DUAN; Meng-tao XING; Yan XU; Xue-fei HAN; Bo LI; Qing-xia ZHAO; Wen-hai YAN

2010, 30(9):  966-970. 

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Objective To explore the protein kinase/protein phosphatase imbalance induced by amyloid β（Aβ） and to observe the intervention role of Ginsenoside Rb1 in neonatal neural cells. Methods The neural stem cells(NSCs) were prepared after isolated from the newborn rat hippocampus and cultured. After 7 d induction the NSCs differentiated into neural cells were randomly divided into three groups: Aβ25~35 group was treated with 20 μmol/L Aβ25~35 for 12 h; Rb1+Aβ25~35 group was preconditioned with 10 μmol/L Rb1 for 24 h and then treated with 20 μmol/L Aβ25~35 for 12 h; control group was given nothing else. RT-PCR and immunofluorescence cytochemistry were used to detect the expression of protein kinase/protein phosphatase and tau. Results Compared with control group, in Aβ25~35 group glycogen synthase kinase 3β(GSK-3β) and cyclin dependent kinase 5(CDK-5) mRNA were higher, protein phosphatase 2A(PP2A) mRNA was lower; the expression of GSK-3β(pY279,216) was higher and PP2A was lower under fluorescence microscope; the positive cell rates of microtubule associated protein Tau(pS396) and Tau(ps262) were higher(p<0.05). Rb1 pretreatment can depress the changes of phosphotase/phosphorylase and tau mentioned above induced by Aβ25~35. Conclusions Aβ25~35 induces phosphotase/phosphorylase imbalance and tau hyperphosphorylation in neonatal neural cells which can be depressed by Rb1.

Overexpression of caveolin-1 induces apoptosis in laryngeal squamous cell carcinoma HEp2 cell line

Dong-hua GU; Jin-liang PING; Qi CHEN; Rong ZHU

2010, 30(9):  971-975. 

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Objective To investigate the effects of caveolin-1 on apoptosis of laryngeal squamous cell carcinoma cell line HEp2. Methods Human caveolin-1 gene were transfected into HEp2 cell line, the positive clones with high expression of caveolin-1 were identified by fluorescence quantitative real time reverse transcriptase-polymerse chain reaction（Real-time RT-PCR）and Western Blot. Cell cycle and apoptosis were analyzed by Flow cytometry. Caspase-3 relative activities were calculated according to the changs of optical density after reaction with caspase-3 substrate. Real-time RT-PCR was used to determine the expression level of survivin mRNA and the expression of bcl-2 and survivin protein were detected by Western Blot. Results the clones stably overexpressing caveolin-1 were obtained, namely HEp2-CAV1. Overexpressing caveolin-1 resulted in the cell cycle arrest at G0/G1 phase and increased the apoptosis cell fraction. Compared with the parental HEp2 cells, the realtive activites of caspase-3 increased by 2.72 fold（p<0.05）, the expression level of survivin mRNA reduced by 74%（p<0.01） and the protein bcl-2 significantly decreased in HEp2-CAV1 cells, respectively. Conclusion overexpression of caveolin-1 induces apoptosis in laryngeal squamous cell carcinoma HEp2 cell line, downregulation of bcl-2 and survivin expression may be involved in its mechanism.

Effect of silencing Hes1 and Hes5 gene on proliferation of human glioma cell line U251

Chun-hua WANG; Ling LIN; Zhi-hong ZHENG

2010, 30(9):  976-980. 

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Objective To study the effect of Hes1，Hes5 gene silencing on proliferation of U251 cells. Methods Lentiviral vectors expressing shRNA targeting human Hes1 and Hes5 gene were constructed to interfere Hes1 and Hes5 gene expression in U251 cells. MTT assay，plate clone formation assay and flow cytometry were performed to assay the proliferation and colony formation ability of U251 cells. Results Proliferation and colony formation of U251 cells can be effectively inhibit by Hes1 or Hes5 gene silencing. Conclusion Hes1 and Hes5 are directly involved in proliferation regulation of U251 cells, and can be regarded as potential gene therapeutic targets of glioma. There are no difference between them in effecting proliferation of U251 cells.

The increased expression of granulysin in peripheral blood of acute viral hepatitis C

Xin-li LI; Jun JU; Xiang-hong XU; Wan-xia WANG

2010, 30(9):  981-983. 

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Objective To investigate granulysin expression in peripheral blood of patients with acute viral hepatitis C and its clinical significance. Methods The level of GNLYmRNA in the PBMC and the content of serum GNLY were determinded by RT-PCR and ELISA, respectively. Results The expression of PBMC GNLYmRNA in patients with acute hepatitis C (7.71 ± 1.44) was higher than that of controls (0.83 ± 0.17) (P<0.01)；and serum GNLY average content (20.16 ± 6.03)ng/mL was also higher than that of controls (9.94 ± 2.99)ng/mL (P<0.01). The level of GNLYmRNA expression in PBMC had negatively correlation with the level of HCV-RNA expression (r=-0.869, P<0.01); the content of serum GNLY and the ALT was positively correlated (r=0.522, P <0.01), and was also positively correlated with AST (r=0.661, P<0.01). Conclusion The GNLY gene and protein expression levels are both higher in patients with acute viral hepatitis C compared with those in normal controls. The change level of GNLY expression may be helpful for determining of the cell immune status of acute viral hepatitis C patients, monitoring the condition of acute viral hepatitis C.

临床园地

A Modified Ultra-long Down-regulation Protocol for Infertile Patients with PCOS in IVF/ICSI-ET

Fei GONG; Ke-li LUO; Guang-xiu LU

2010, 30(9):  984-987. 

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Objectives To compare and analyze the effect of two controlled ovarian stimulation (COH) protocols (Modified ultra-long down-regulation protocol vs. conventional long down-regulation protocol) for In Vitro Fertilization / Intracytoplasmic Sperm Injection - Embryo Transfer (IVF/ICSI-ET) treatment in infertility patients with Polycystic Ovarian Syndrome (PCOS).Methods 132 IVF/ICSI-ET cycles utilizing modified ultra-long down-regulation protocol and 588 IVF/ICSI-ET cycles underwent conventional long down-regulation protocol during August 2008 to December 2009 were retrospectively analyzed. Several key parameters of the two COH protocols, including: fertilization and cleavage rate, clinical pregnancy rate, live birth rate, abortion rate, treatment duration and therapeutic expenses etc. were compared and analyzed. Results In modified ultra-long down-regulation protocol, the fertilization and cleavage rate, clinical pregnancy rate and implantation rate were significantly increased, P<0.001, as well for the live birth rate (P<0.05), the abortion rate (P<0.05) and the progesterone level on the day of HCG injection were decreased (P<0.001). In the mean while, the treatment duration was shortened (P<0.001), and treatment cost were reduced (P<0.001), when compared to conventional long protocol. Conclusion Modified super-long protocol plus hMG for PCOS infertile patients saved the therapeutic cost and raised the success rate of ART.

Benign recurrent intrahepatic cholestasis

Dong-wen CHEN; Dong WU; Xiao-hong SUN; Li-ming ZHU; Gang SUN

2010, 30(9):  988-990. 

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Objective We try to make a thorough and systemic review on benign recurrent intrahepatic cholestais (BRIC). Methods We made a brief introduction on 1 case of BRIC in Peking Union Medical College Hospital and then browsed qualified BRIC cases on internet databases. Result We found 6 cases in China and 21 cases from abroad. BRIC is characteristic by recurrent cholestasis and pruritus with normal manifestation, biochemical sign and imaging during the interval. The clinical course in most cases is benign, with relatively few cases develop progressive familial intrahepatic cholestasis (PFIC) and have a bad prognosis.

研究短文

Pre-miR-15a enhances the sensitivity of Raji cells to Ara-C

Qin CHEN; Dong-mei HE

2010, 30(9):  991-992. 

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Increased expression of SPARC in cervical carcinoma

Xuan LIU; Zhu-mei CUI; Ye ZHENG; Zhi-hua LIU; Li-li YAN

2010, 30(9):  993-994. 

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短篇综述

Aquaporin-1 and Endothelial Function

Xiang-dong LI; Yue-jin YANG

2010, 30(9):  995-998. 

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Aquaporin-1 (AQP1), the only water channel that expressed in most microvascular endothelia outside of brain, plays an important role in the mechanism of urinary concentration in outer modularly descending vasa recta, in chylomicron absorption in lacteal endothelia, and in fluid balance across the corneal endothelium. In vascular endothelium, AQP1 is closely related to nitric oxide (NO) and CO2 transport, vascular function of dilatation and contraction, myocardial edema and necrosis after myocardial ischemia, angiopoiesis, migration of endothelial cells and tumor spread.

Progress in ClC Mutant and its related Diseases

Hai-feng ZHANG; Zhi-quan BAI; Li-xin CHEN; Li-wei WANG

2010, 30(9):  999-1001. 

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ClC are chloride family proteins helping chloride ion transmembrane, which participated in a variety of physiological and pathological processes. Dysfunctional ClC protein induced by gene mutation could change the characters of channel gate and disturb chloride ion transmembrane, resulting in congenital myotonia, idiopathic epilepsy, Bartter's syndrome, Dent's disease, retina degeneration, osteopetrosis etc.

Research progress of the correlation between Hippo pathway and tumorigenesis

Gui-lan LI; Li ZHANG; Hua-chao YAN

2010, 30(9):  1002-1004. 

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The Hippo kinase pathway is a newly described growth regulatory pathway and restricts both cell growth and proliferation, as well as to induce apoptosis, the inactivation or deregulation of the pathway might be particularly advantageous to cancer cells. Several tumor suppressor genes and one candidate oncogene have been implicated as members of this pathway, YAP is a candidate oncogene, which is observed to overexpress in several human cancers, and RASSF or MER functions as tumour suppressor gene and loss-of-function mutation of them has been observed in several human cancer cell lines.

Androgen Receptor Gene Knockout Models and Spermatogenesis

Xin-yu ZHANG; Han-zhong LI; Hong-jun LI

2010, 30(9):  1005-1009. 

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Androgens play an important role in male spermatogenesis.These steroid hormones function through binding to intracellular androgen receptors(ARs).The abnormality of structure and function of ARs may influence spermatogenesis.To obtain a better insight into molecular mechanisms of ARs in various testicular cells and its impact on spermatogenesis, several testicular cell type-specific AR knockout mouse models have been generated and this rview summaries studies using these animal models.