Basic & Clinical Medicine ›› 2010, Vol. 30 ›› Issue (9): 971-975.

• 研究论文 • Previous Articles     Next Articles

Overexpression of caveolin-1 induces apoptosis in laryngeal squamous cell carcinoma HEp2 cell line

Dong-hua GU, Jin-liang PING, Qi CHEN, Rong ZHU   

  1. Affiliated Hospital of Huzhou Teachers College Shanghai Medical College, Fudan University
  • Received:2009-10-23 Revised:2009-12-29 Online:2010-09-05 Published:2010-09-05
  • Contact: Dong-hua GU,

Abstract: Objective To investigate the effects of caveolin-1 on apoptosis of laryngeal squamous cell carcinoma cell line HEp2. Methods Human caveolin-1 gene were transfected into HEp2 cell line, the positive clones with high expression of caveolin-1 were identified by fluorescence quantitative real time reverse transcriptase-polymerse chain reaction(Real-time RT-PCR)and Western Blot. Cell cycle and apoptosis were analyzed by Flow cytometry. Caspase-3 relative activities were calculated according to the changs of optical density after reaction with caspase-3 substrate. Real-time RT-PCR was used to determine the expression level of survivin mRNA and the expression of bcl-2 and survivin protein were detected by Western Blot. Results the clones stably overexpressing caveolin-1 were obtained, namely HEp2-CAV1. Overexpressing caveolin-1 resulted in the cell cycle arrest at G0/G1 phase and increased the apoptosis cell fraction. Compared with the parental HEp2 cells, the realtive activites of caspase-3 increased by 2.72 fold(p<0.05), the expression level of survivin mRNA reduced by 74%(p<0.01) and the protein bcl-2 significantly decreased in HEp2-CAV1 cells, respectively. Conclusion overexpression of caveolin-1 induces apoptosis in laryngeal squamous cell carcinoma HEp2 cell line, downregulation of bcl-2 and survivin expression may be involved in its mechanism.

Key words: laryngeal tumor, caveolin, apoptosis