Basic & Clinical Medicine ›› 2010, Vol. 30 ›› Issue (9): 976-980.

• 研究论文 • Previous Articles     Next Articles

Effect of silencing Hes1 and Hes5 gene on proliferation of human glioma cell line U251

Chun-hua WANG, Ling LIN, Zhi-hong ZHENG   

  1. Research Center of Molecular Medicine, Fujian Medical University Research Center of Molecular Medicine, Fujian Medical University
  • Received:2010-01-11 Revised:2010-01-27 Online:2010-09-05 Published:2010-09-05
  • Contact: Zhi-hong ZHENG

Abstract: Objective To study the effect of Hes1,Hes5 gene silencing on proliferation of U251 cells. Methods Lentiviral vectors expressing shRNA targeting human Hes1 and Hes5 gene were constructed to interfere Hes1 and Hes5 gene expression in U251 cells. MTT assay,plate clone formation assay and flow cytometry were performed to assay the proliferation and colony formation ability of U251 cells. Results Proliferation and colony formation of U251 cells can be effectively inhibit by Hes1 or Hes5 gene silencing. Conclusion Hes1 and Hes5 are directly involved in proliferation regulation of U251 cells, and can be regarded as potential gene therapeutic targets of glioma. There are no difference between them in effecting proliferation of U251 cells.

Key words: Hes1, Hes5, glioma, RNAi, lentivirus