Table of Content

05 August 2010, Volume 30 Issue 8

Previous Issue    Next Issue

研究论文

The Relationship between AQP4 and Kir4.1's expressions and Brain Edema after Cerebral Ischemia Reperfusion

Xing-ye ZHANG; Shan-quan SUN; Hui LIU; Guo-ping QIU; Fei ZHUO; Jing LI; Bao-bing GAO

2010, 30(8):  785-789. 

Asbtract ( 1945 )   PDF (2053KB) ( 727 )  

Related Articles | Metrics

Objective The purpose of this study was to investigate the change of AQP4 and Kir4.1's expression to explore the mechanism of brain edema after CIR. Methods A total of 136 healthy SD rats were randomly divided into two groups , CIR group and control group.CIR models were established by occluding unilateral middle cerebral artery of the rats with suture method.The sutures were removed after two hours to allow reperfusion.The brains of the rats were taken out at 6h、12h、24h、48h、72h and 7d after reperfusion.The change of brain water content(BWC) was measured by the wet and dry weight .AQP4 and Kir4．l were detected by immunofluorescence．The contents of AQP4 and Kir4．1 mRNA were detected by RT-PCR．And calculated the ratios of AQP4 mRNA and Kir4．1 mRNA Results The BWC of ischemic brain tissue increased form CIR 6h ,and reached its peak during the CIR 48-72h. The permeability of BBB increased after CIR,the increasing permeability had two peaks,at CIR12h and CIR 48h respectively。AQP4 and Kir4．1 were co-expressed at cerebral pia mater、choroid plexes and ependyma 。The expression of AQP4 and Kir4.1mRNA increased after CIR.The ratio of AQP4 and Kir4．1 mRNA began to increased at 12 h after CIR．Conclusions In the early stage after CIR,The increased capillary vessel permeability induced the brain edema;In the later stage．The redistribution of AQP4 and Kir4．1 played an important role in the pathological course of CIR brain edema.

Analysis of the relationship between serum uric acid and metabolic syndrome in Beijing adults

Zhi-xin BIE; Wei-gang FANG; Xiao-ming HUANG; Yu WANG; Wei-guo ZHU; Jia-lin CHEN; Xue-jun ZENG

2010, 30(8):  790-794. 

Asbtract ( 1596 )   PDF (540KB) ( 830 )  

Related Articles | Metrics

Objective To evaluate the relationship between serum uric acid and metabolic syndrome in Beijing adults. Methods We conducted a study in a cohort of 2174 individuals (1280 men and 894 women) who participated in annual health examinations at the Peking Union Medical College Hospital from September to December 2005. We analysed the relationship between serum uric acid and metabolic syndrome. Results Serum uric acid levels increased in groups of individuals with more components of metabolic syndrome. The risk of metabolic syndrome showed a graded increase according to serum uric acid levels, the risk of metabolic syndrome (odds ratio, 95% CI) in sex-specific quartiles of serum uric acid was 1.90 (1.26～2.86), 2.66 (1.79～3.94), and 5.06 (3.35～7.65) in men, and 1.66 (1.03～2.68), 2.33 (1.42～3.82), and 4.65 (2.91～7.43) in women. Conclusion Serum uric acid level is an independent risk factors associated with metabolic syndrome. Further studies are needed to explore the mechanism for the close relationship between them.

Cigarette smoke extracts upregulate the expression of γ－glutamylcysteine synthetase via aPKCι/ζ-NRF2 signaling pathway in the bronchial epithelial cells of rats

Gang JIANG; Ai-guo DAI; Rui-cheng HU

2010, 30(8):  795-800. 

Asbtract ( 1974 )   PDF (976KB) ( 801 )  

Related Articles | Metrics

Objective To observe the effect of the signal pathway of aPKCι/ζ (antypical protein kinase Cι/ζ)-NRF2 (nuclear factor-Ｅ2 related factor) on γ-glutamylcysteine synthetase（γ-GCS）of the bronchial epithelial cells of rats after exposure to cigarette smoke extracts（CSE）. Methods γ-GCS, NRF2 and p-aPKCι/ζ proteins were semi-quantified by Western blot. The expression of γ-GCS protein was assessed by immunocytochemistry. γ-GCS mRNA expression were detected by reverse transcription-polymerase chain reaction (RT-PCR). NRF2 protein was observed by immunofluorescence. Results NRF2 protein mainly expressed in the nucleus, NRF2 protein of nucleus strongly expressed after exposure to CSE for 3 hours. p-aPKCι/ζ protein significantly enhanced in CSE 3h group than the control group（P＜0.05）. γ-GCS protein and mRNA were significantly increased in CSE 3h group as compared to the control group（P＜0.05）.Pretreated with aPKCι/ζ inhibitor RO318220, NRF2 protein of cytoplasm strongly expressed, p-aPKCι/ζ protein, γ-GCS protein and mRNA were significantly decreased than CSE3h group（P＜0.05）. The correlation analysis demonstrated that there were a positive correlation between NRF2 and γ-GCS and p- aPKCι/ζ, and between p-aPKCι/ζ and NRF2 and γ-GCS（P＜0.05）. Conclusion CSE might upregulate γ-GCS expression through aPKCι/ζ -NRF2 signaling pathway in the bronchial epithelial cells of rats.

MiR-141 increases proliferation and migration of human vascular endothelial cells

Da-quan LIU; Dong-hua LI; Hong-bin LIU

2010, 30(8):  801-806. 

Asbtract ( 2401 )   PDF (863KB) ( 1150 )  

Related Articles | Metrics

Objective In this study, the effects of microRNA on the proliferation and migration of endothelial cells were researched in order to illuminate the molecular mechanisms of microRNA in angiogenesis. Methods MicroRNA was blocked in endothelial cells and the changes of cell proliferation were detected with methyl thiazolyl tetrazolium (MTT) assay. Subsequently, microRNA was blocked or overexpressed in endothelial cells and the changes of cell phenotypes were detected with MTT assay, colony-forming test and migration assay. Results Consequence of MTT assay demonstrated that 7 microRNAs increased growth activity of endothelial cells. With suppression of miR-141, the endothelial cells proliferation, migration and the colony formation activity were both inhibited significantly. However, the changes of cell phenotypes were reversed when miR- 141 was overexpressed. Conclusion These results suggest that miR-141 promotes proliferation and migration of endothelial cells and increases angiogenesis.

The Detection of HPV16 L1 in Infected Cervical Specimen

Yan-mei SHI; Chang-yi XIAO; Hong YE; Ya-qin WANG; Jiang-feng WU

2010, 30(8):  807-810. 

Asbtract ( 2268 )   PDF (742KB) ( 869 )  

Related Articles | Metrics

Objectives Detecting the expression of L1 in HPV16 infected cervical tissues and unearthing the correlation of HPV16 L1 with cervix injured degree. Methods Multiplex PCR was used to detect HPV16-positive specimen in cervicitis, CINⅠ~Ⅱ,cancer in situ and cervical cancer. The expression of L1 was detected in each group by ELISA and Immunohistochemistry. Results 46 out of 54 cases were HPV16 positive(85.2%). The reaction between antigen and antibody was lower as the aggravated of cervical damage in ELISA; the positive reaction appeared in the epithelium tissue in Immunohistochemistry. which effected by tissue`s damaged degree and differentiated states. Conclusion There are higher rate of HPV16 detection in women. L1 expressed in epithelium which affected by tissue`s differentiation. The expression of HPV16 L1 was lower as the damage aggravated. Detecting HPV L1 earlier in clinic can provide the direction for cervical cancer.

The detection of gene polymorphisms of MTHFR C677T and A1298C in severe depression patients

Lei-guang FENG; Chun-qing SHAO; Ying-hui LIU; Yong-hong TAO; Xiao-lei HAO

2010, 30(8):  811-814. 

Asbtract ( 2076 )   PDF (511KB) ( 866 )  

Related Articles | Metrics

Objective Investigating the relationships of polymorphisms of 5,10-MTHFR gene and severe depression in northern Chinese Han population provided reliable evidences for the prevention and cure of diseases. Methods Using case-control study,We detect the gene polymorphisms of MTHFR C677T and A1298C by polymerase chain reaction-Restriction fragment length polymorphism (PCR-RFLP). Results ①The frequencies of genotypes of 677TT in control groups and that of T alleles were 13.16% and 39.80%;the frequencies of genotypes of 1298CC and that of C alleles were 1.32% and 12.83%;②The frequency of MTHFR 677T/T genotype (35.53%) and 677T allele (57.24%) in depression were respectively higher than that in control group(13.16%)(P<0.001);the differences of the frequencies of MTHFR A1298C genotype and C allele between control group were respectively no statistically significant(P<0.05);③The Ligistic regression analysis showed that the genotype of C677T was relative to the occurrence of disease,and the genotype of A1298C wasnot relative to the occurrence of disease. Conclusions The mutation of MTHFR C677T in the groups of severe depression patients was relative to the occurrence of severe depression disease,and it is the risk factors of disease;the mutation of MTHFR A1298C wasnot relative to the occurrence of the severe depression.

Construction of TK expression vector regulated by hypoxia and radiation and its radiosensitization effect on human breast cancer cells

Ling WEI; Xian-rang SONG; Xing-wu WANG; Ju-jie SUN; Bao SONG; Yan ZHENG

2010, 30(8):  815-819. 

Asbtract ( 1686 )   PDF (901KB) ( 793 )  

Related Articles | Metrics

Objective To construct adenovirus vector Ad.HRE.CArG.HSV-TK regulated by hypoxia and radiation via AdEasy system and to observe its radiosensitization effect in human breast cancer cell lines Bcap37 and MDA-MB-435.Methods Using molecular clone technologies, Ad.HRE.CArG.HSV-TK inserted with HRE.CArG.HSV-TK fragment was constructed. The virus was purifed by CsCl gradient centrifuge. The functional titer of adenovirus was detected by GFP tag expression. Cell lines of Bcap37 and MDA-MB-435 were transducted with adenovirus in vitro, 48h later, expression rate of GFP was detected by flow cytometry.TK gene mRNA and protein expression were detected with real -time RT-PCR and Western blot respectively.The growth inhibition effect and sensitizer enhancement ratio( SER)of adenovirus（Ad）transduction combined with radiation（RT） were detected with MTT assay.Results The final titers of adenovirus was 2.1×109TU/ml. Bcap37 and MDA-MB-435 cells transducted by 50 MOI adenovirus in vitro for 48h, positive rate of GFP was 92% and 93%,respectively.TK expression increased dramatically（P<0.05）. Growth inhibition rate in group of Ad＋RT was significantly higher than that in Ad group and the same dose of RT group（P<0.05）. SER were both 1.55.Conclusion The recombinant adenovirus expressing GFP and TK gene can be generated via AdEasy system.The adenovirus has radiosensitization effect on breast cancer cells. It may serve as a basis for later gene radiotherapy research in breast cancer.

Simvastatin promotes the osteoblast differentiation of rat bone marrow stromal cells

Fa-ming TIAN; Liu ZHANG; Ya-qiang MENG; Ying-ze ZHANG

2010, 30(8):  820-825. 

Asbtract ( 1902 )   PDF (824KB) ( 956 )  

Related Articles | Metrics

Objective To investigate the effects of simvastatin on osteoblast differentiated rat bone marrow stromal cells(BMSCs), as well as the differently expressed genes associated with osteogenesis of the BMSCs during this process. Methods Bone marrow stromal cells from the femurs and tibias of 6-week old rats were cultured in vitro, three days later the cells were treated with simvastatin(1×10-7mol/L)(group SIM) or vehicle(group V). Alkaline phosphatase activity was assessed at 14th day, the following assays were performed at 21th day: Von Kossa staining for observing extracellular matrix mineralization; total RNA was extracted for detecting the gene expression profiles by Oligonucleotides microarray chip and were confirmed by real-time quantitative RT-PCR. Results 1.Cells in SIM showed significantly higher ALP activity and ability of extracellular matrix mineralization compared with those of group V;（p<0.05） 2.Oligonucleotides microarray chip analysis showed that 678 genes out of 22,575 rat genes had differential expression (≥2 fold or ≤0.5 fold), including genes known to be related to osteogenesis, such as , ALPl、TGFβ1、OCN、DLX5、Axin2、BMP-2、IBSP、MMP13, etc. Conclusion Simvastatin could promote the osteogenic differentiation of BMSCs in vitro, many genes associated with osteogenesis may participate in this process.

The regulation of telomerase activity and telomere stability by MiR-138 in MCF-7 cells

Rong SHI; Li JIANG; Yang GAO; Jue-yu ZHOU; Da-peng DING; Wen-ling ZHENG; Wen-li MA

2010, 30(8):  826-830. 

Asbtract ( 2050 )   PDF (860KB) ( 1005 )  

Related Articles | Metrics

Objective To study the regulation of telomerase activity of MiR-138 through targeting HTERT as the downstream molecular, and its effect on telomere stability in breast cancer cell line MCF-7. Methods MiR-138 mimics were transiently transfected into breast cancer cell line MCF-7. MTT method was applied for detecting the effect on cell proliferation. The HTERT expression level of the cells was detected by real-time RT-PCR and telomerase activity was checked by TRAP Assay 48h after transfection. Meanwhile, the cells were checked simultaneously by 53BP1 antibody immunofluorescent dyeing and telomere FISH dyeing. Results The HTERT expression level of MCF-7 cells treated with MiR-138 was repressed by 2.18 folds (2-△△Ct) when compared with the control cells 48h after transfection. Telomerase activity was also decreased by 2.69 folds when compare with the control cells. 53BP1congregated into many Foci and co-localized with the telomere spots at a percentage of 20.62%±1.55%. Conclusion MiR-138 can regulate telomerase activity in MCF-7 cells by targeting HTERT as the downstream molecular, and affects the telomere stability as well.

Improvement of Coeloglossum viride var. bracteatum extract in IBO-induced AD rats

Jie WANG; Cai-yuan YU; Jian-jun ZHANG

2010, 30(8):  831-835. 

Asbtract ( 1910 )   PDF (734KB) ( 805 )  

Related Articles | Metrics

Objective To study the effect of Coeloglossum viride var. bracteatum extract (CE) on the learning and memory ability in dementia rats by ibotenic acid (IBO), and explore its mechanism. Methods IBO (2 μl, 8 g/L) was stereoscopic injected into unilateral nbM of SD rats to produce AD-mimic animal model. CE was given to rat by intragastric administration (5mg/kg) 1h before the stereoscopic operation, and after the operation once a day. The Morris water maze test and the step down test was taken. The expressions of acetylcholinesterase (AChE), choline acetyltransferase (ChAT), nerve growth factor (NGF) and tyrosine kinase (TrkA) in the hippocampus, cortex and striatum were examined by Western blot. Results CE significantly improved the learning and memory ability in IBO-induced AD-mimic rats. CE treatment shortened escape latency significantly as compared with nbM-lesion rats. The expression of AChE increased significantly in nbM-lesion rat hippocampus; CE inhibited the excessive expression of AChE. Conclusion CE could improve the dysfunction of learning and memory ability in AD animals by inhibiting the excessive expression of AChE in hippocampus under the pathological condition.

The investigation apoptosis of hepatic stellate cell line of rat bone marrow mesenchymal stem cells in vitro

Zi-yu LIANG; Hai-xing JIANG; Shan-yu QIN; Dong-xu WANG; Si-biao SU

2010, 30(8):  836-842. 

Asbtract ( 1881 )   PDF (1350KB) ( 1009 )  

Related Articles | Metrics

Objective To investigate the effect of Rat bone marrow mesenchymal stem cells (MSCs) on apoptosis of hepatic stellate cells (HSCs) by co-cultured, and explore the underlying mechanism of MSCs on the HSCs. Methods MSCs were isolated and cultured from bone marrow in rats . HSCs and fiberoblast cells were recoveried and activated morphologically，a-SMA expression in HSCs was evaluated immuno-histochemically. The co-culture of MSCs with HSCs were performed by using a 6-well Transwell membranes (24mm diameter, 0.4 μm pore size).The HSCs were seeded in the lower chamber with MSCs and fiberoblast cells (2×105cells /ml) were seeded onto the Transwell membrane of the inner chamber.Cultures were maintained in HSCs in medium for 24h、48h and 72h. Three groups were divided: ①HSCs control group②fiberoblast control group③MSCs group. At the different pointed time, the inhibitory rate of HSCs proliferation with MSCs co-culture were tested by the method of WST-8, and the rate of apoptosis of HSCs were detected by Annexin-V-FITC/PI and DNA Ladder, and the HSCs in co-culture system were collected and the mRNA expressions of Caspase-3,Bax in HSCs were detected by RT- PCR . The protein expressions of Caspase-3,Bax in HSCs were evaluated by Western blot. Results With the co-culture time lasting, MSCs group showed the strip of DNA Ladder, the inhibitory rate of HSCs ,the rate of apoptosis of HSCs and the mRNA expression of Caspase-3,Bax and Caspase-3,Bax protein in MSCs group were significantly increased at time 24h, which were significant higher than control groups(P<0.01）, and the same effection was taken place at the following times. Conclusions MSCs could induce apoptosis of HSCs in vitro by up-regulating the expression levels of Caspase-3 and Bax, and thus play important role in the antifibrosis

The Association of I/D Genetic Polymorphism of ACE and Plasma Lipid with Essential Hypertension

Xiu-ling HAN; Zhi-xia SHEN; Hong-fen LI; Zhi-fang ZHANG; He-yong WANG; Shu-qing ZHANG; Shou-ling WU

2010, 30(8):  843-846. 

Asbtract ( 1927 )   PDF (480KB) ( 798 )  

Related Articles | Metrics

Objective To study the relationship between angiotensin converting enzyme(ACE) gene dyslipemia and Essential Hypertension.Methods To examinate the I/D Polymorphism of ACE gene by PCR .Biochemical indexex included of TC、TG、HDL、LDL and VLDL were detected by autochiemical emalyzer. Results The HDL was higher in II type than the others; The LDL was higher in ID、DD types than the II type.The morbidities of EH were different among three gene types, the morbidity of EH was higher in ID (19.88%)、DD (20.82%) types than the II (14.26%) type(p<0.05);There was a significant difference in the distribution of ACE genetypes and allele frequency in different cohort who were EH or not (P<0.05,P<0.01).The lipemia was different between EH group and NEH group,except HDL ,and LDL in ID、II genetypes.In NEH group,the TC was higher in II type than the DD type;The LDL was higher in ID、DD types than the II type. Conclusion The morbidity of EH were different among three gene types.Polymorphism of ACE gene has some relation of lipemia levels such as TC、HDL、LDH .

The repressive effects of HTERT interference on γ ray induced DNA damage response in MCF-7 cell

Rong SHI; Wen-li MA; Yang GAO; Jue-yu ZHOU; Da-peng DING; Hai-lang YU; Wen-ling ZHENG

2010, 30(8):  847-851. 

Asbtract ( 1834 )   PDF (1184KB) ( 887 )  

Related Articles | Metrics

Objective To study the effect of HTERT RNA interference on γ ray irradiation induced DNA damage response in breast cancer cell line MCF-7. Methods A retrovirus carrying HTERT- siRNA was used to repress the expression of telomerase catalyze unit HTERT in breast cancer cell line MCF-7. The HTERT expression level of the cells was confirmed by real-time RT-PCR and Western blot. 3 Gy dosage of γ ray from 137Cs was applied to irradiate the cells, cells were then collected and fixed before and 1, 2, 4, 8 and 12h after irradiation, and Phospho-53BP1 antibody was used for immunofluorescent dyeing of the cells. Cells were also collected before and 1h, 4h, 12h after irradiation and phosphorylation level of 53BP1 was detected by Western blot as well. Results The HTERT expression level of Cells treated with HTERT -siRNA was repressed by 3.20 folds (2-△△Ct) when compared with the control cells. The HTERT protein level also decreased by 2.56 folds. DNA damage response of cells treated with HTERT -siRNA was repressed obviously. Conclusion DNA damage response induced by γ ray irradiation in breast cancer cell line MCF-7 could be repressed through RNA interference of HTERT expression level.

High glucose-induced up-regulation of osteopontin in rat aortic vascular smooth muscle cells

Wei JIE; Bo-tao LUO; Han-guo JIANG; Jin CHEN; Zhi-hua SHEN

2010, 30(8):  852-856. 

Asbtract ( 1841 )   PDF (1076KB) ( 781 )  

Related Articles | Metrics

Objective To investigate the effects and mechanism of high glucose on the osteopontin (OPN) expression in rat aortic vascular smooth muscle cells (VSMCs). Methods SD rats were intraperitoneally injected with streptozotocin, OPN and PCNA expression in rat thoracic aortas were detected by immunohistochemical staining, RT-PCR and western blot analysis; After primary rat aortic VSMCs treated with DMEM medium supplemented with normal glucose (5.5mmol/L), high glucose (25mmol/L), mannitol (25mmol/L), high glucose + GF109203X (PKC inhibitor, 10μmol/L) and high glucose + NAC (N-acetyl-l-cysteine, NF-κB inhibitor, 10μmol/L) for 48 h, OPN expression was examined by immunofluorescence and western blot, expression of PCNA, PKC, and p65 were assessed by western blot analysis. Results OPN and PCNA expression were increased in hyperglycemic rat aortic VSMCs. Expression of OPN, PCNA, PKC, and p65 were significantly up-regulated in VSMCs exposed to high glucose. Expression of PCNA was attenuated by GF109203X and NAC in high glucose-treated VSMCs, while the expression of OPN was only inhibited by GF109203X. Conclusion High glucose induces OPN expression and cell proliferation in VSMCs may be due to the activation of PKC signal, which play a critical role in diabetic vascular complications.

The correlation of AR expression and AR gene gain/lose with tumor prognosis in prostate cancer

Zhi-en ZHOU; Han-zhong LI; Wei-gang YAN

2010, 30(8):  857-861. 

Asbtract ( 2051 )   PDF (1058KB) ( 853 )  

Related Articles | Metrics

Objective To study the clinical value of AR expression and its gene gain/lose in evaluating the prognosis of prostate cancer. Methods 19 patients were aged 58～90 years, and their PSA level before therapy were (13.3～120)ng/mL. As for gleason score, two had high differentiation (gleason score<7) and 17 had low differentiation (gleason score>=7).Using immunohistochemical and FISH method, we analyzed the expression of AR and its gene gain/lose in PC tissues from 19 samples. The correlation of AR expression and AR gene gain/lose with tumor prognosis in prostate cancer were analyzed in combination with the clinical data. Results 2 of 19 were found AR gene gain, the positive rate was 10.5%. The two positive samples were both from the survival group. However, none of the 6 samples from the death group was found AR gene gain. AR positive intensity of the unrecurent group were significantly higher than the death group(P<0.05). Conclusions Strong positive degree of AR expression in immunohistochemical test and AR gene gain occurring before therapy may be indicators for favorable prognosis in prostate cancer.

Identification and the humanization of antibody from anti-Aβ murine monoclonal antibody against Alzheimer's disease

Yan-wei LI; De CHANG; Xue-mei ZHAO; Ping LIANG

2010, 30(8):  862-867. 

Asbtract ( 2055 )   PDF (799KB) ( 1002 )  

Related Articles | Metrics

Objectives　Humanize the Frame regions in Variable regions of the murine monoclonal antibody against Alzheimer's disease, to establish a basis for clinic application in the future. Methods　Based on the chimeric antibody constructed previously, we humanized the frame regions of the heavy chain and the light chain in variable regions using template-replacement method. Eukaryotic expression vectors were got by inserting humanized antibody genes of the heavy and light chains into vectors. Then the eukaryotic expression vectors were co-transfected into CHO cells using liposome fusion method. The tests of characters include the titer and expression amount using ELISA, as well as molecular weight using SDS-PAGE and Western Blot and biological activity tested by immunohistochemistry. Results　The heavy chain of the recombinant antibody was about 1500bp and the light chain was about 750bp, which was in accordance with our anticipation. The expression amount of the product was 1.11mg/L. The molecular weight of the humanized antibody was about 51ku(heavy chain) and 25ku(light chain). The expressed product can bind Aβ antigen specifically tested by ELISA(A value of the humanized antibody: 2.24), keeping the same as that of the chimeric antibody basically. The expressed product can only be recognized by goat-anti-human antibody rather than goat-anti-mouse antibody. The results also showed the expressed product can bind Aβ antigen specifically tested by immunohistochemistry. Conclusions The humanized antibody keeps the same as the chimeric antibody in biological activity basically, this provides a possibility for its clinic application for Alzheimer's disease in the future.

技术与方法

Construction of expression vector of autocrine motility factor receptor and it's RNAi

Yan SUN; Wen-xia ZHOU; Yong-xiang ZHANG

2010, 30(8):  868-872. 

Asbtract ( 1768 )   PDF (1020KB) ( 797 )  

Related Articles | Metrics

Objective To construct the expression vector of AMFR gene and AMFRsiRNA, and investigate the effect of AMFRsiRNA on the expression of AMFR gene. Methods AMFR gene was amplified by PCR technique and the product of PCR was inserted into the pcDNA3-FLAGC eukaryotic expression vector. Two AMFRsiRNAs were designed by RNAi technique and the AMFRsiRNAs were inserted into the pSliencer 2.1-U6 neo expression vector. Then human embryo kidney 293T cells were co-transfected with the AMFRsiRNA expression vectors and FLAG-tagged AMFR expression vector. The effect of AMFRsiRNAs on the expression of AMFR gene was identified by Western blot. Results The expression vectors of AMFR gene and AMFRsiRNAs were constructed and confirmed by restriction enzyme digestion and DNA sequence analysis. Western blot showed that AMFRsiRNAs could effectively inhibit the expression of AMFR gene. Conclusions The expression vectors of AMFR gene and AMFRsiRNAs were constructed successfully, and the AMFRsiRNAs could effectively inhibit the expression of AMFR gene.

临床园地

Clinical investigation and gene analysis of one pedigree with multiple endocrine neoplasia type 2A

Wen WEI; Xiao-ying LIU; Xiao-hua JIANG; Yi-chuan LIN; Li-bin LIU

2010, 30(8):  873-876. 

Asbtract ( 1518 )   PDF (768KB) ( 713 )  

Related Articles | Metrics

Objective To carry out a clinical investigation and gene analysis of one pedigree with multiple endocrine neoplasia type 2A(MEN2A). Methods Carried out a clinical investigation of one MEN2A family,which has 24 members including the proband.The DNAs of the 16 members from the family were extracted from blood leukocytes,PCR and gene sequencing of PCR products by an automated DNA sequencer were applied to scan the exonl0 and 11 of the RET proto-oncogene. Results 1、The pedigree of this index case had one patient with multiple endocrine neoplasia type 2A,who refused to be phlebotomized for examining;Three doubtful patients had died,so could not have the gene analysis;Genetic screening identified the same mutation in a family member,who then died with chest distress and pallor suddenly at home.2、A missense mutation of TGC (Cys) to CGC (Arg) at codon 634 in exon 11 of the RET proto-oncogene was detected in the index case and a family member. Conclusion The mutation (C634R) is detected in the family with MEN2A.Early RET mutation analysis should be performed routinely in all MEN2A, and screening methods may be used in analyzing familymembers at risk.

研究短文

Epstein-Barr Virus Infection Enhances the Expression of BCL-2 Gene in Gastric Carcinoma Cell Line NU-GC-3

Yong-zhan ZHEN; Tian-ji ZHOU; Guang-ling ZHANG; Li-hua ZHU; Jun-ling GAO

2010, 30(8):  877-879. 

Asbtract ( 1263 )   PDF (580KB) ( 767 )  

Related Articles | Metrics

Association of renal injury in rats with hypothyroidism and podocyte injury

Zhan CHANG; Juan LI; Qian WANG; Song-yun ZHANG; Rui-ying WANG

2010, 30(8):  880-881. 

Asbtract ( 1426 )   PDF (453KB) ( 813 )  

Related Articles | Metrics

短篇综述

Hydrodynamic gene delivery

Xue-kun XING; Cun-shuan XU

2010, 30(8):  882-884. 

Asbtract ( 2313 )   PDF (441KB) ( 819 )  

Related Articles | Metrics

Foreign gene targeting transfection into vivo and highly expression are the fundation of investigating gene function and gene therapy. The hydrodynamics-based transfection founded in recent years can deliver plasmid into animal cells conveniently, quickly, safely, efficiently, and gene can highly, stably express. This paper introduces the concept, mechanism and application of hydronamic gene delivery.

The mechanism of mesenchymal stem cell therapy for diabets

Hong-bin XIE; Hui QI; Fu-rong LI

2010, 30(8):  885-888. 

Asbtract ( 1935 )   PDF (492KB) ( 1051 )  

Related Articles | Metrics

Mesenchymal stem cells (MSCs) after transplantation to diabetic rats reduced blood glucose levels quickly and effectively, reversed the symptoms of high blood sugar in diabetic rats. The mechanism that MSCs treate diabetes may be associated with transdifferentiation, cell fusion,DNA methylation, paracrine mechanism and endogenous pancreatic stem cells.In this paper, we write a review about the current mechanism of mesenchymal stem cell therapy for diabetes

医学教育

The innovation of experimental pathology teaching system

Xiao-li WEI; Gen-you YAO; Ren ZHOU; Ling-ling WANG

2010, 30(8):  889-891. 

Asbtract ( 1723 )   PDF (519KB) ( 793 )  

Related Articles | Metrics

In order to keep step with the times and adapt to the needs of training innovative compound medical talents in the New Century, we dare to innovate experimental pathology teaching system, reform curriculum and assessment methods, actively encourage the bilingual teaching and practice teaching, reform teaching methods by comprehensively utilizing digital microscope mutual system and digital slides, and establish a brand-new teaching system, then promote the comprehensive development of experimental pathology teaching.

协和大查房

Recurrent menorrhea for 6 years and worsening with bilateral lower extremities edema for 2 months.

Jun-ling ZHUANG; Ying LI; Ying XU; Shu-jie WANG; Qian WANG

2010, 30(8):  892-896. 

Asbtract ( 1277 )   PDF (756KB) ( 821 )  

Related Articles | Metrics

无