Basic & Clinical Medicine ›› 2010, Vol. 30 ›› Issue (8): 807-810.

• 研究论文 • Previous Articles     Next Articles

The Detection of HPV16 L1 in Infected Cervical Specimen

Yan-mei SHI, Chang-yi XIAO, Hong YE, Ya-qin WANG, Jiang-feng WU   

  1. Medical College of China Three Gorges University
  • Received:2009-04-27 Revised:2009-11-12 Online:2010-08-05 Published:2010-08-05

Abstract: Objectives Detecting the expression of L1 in HPV16 infected cervical tissues and unearthing the correlation of HPV16 L1 with cervix injured degree. Methods Multiplex PCR was used to detect HPV16-positive specimen in cervicitis, CINⅠ~Ⅱ,cancer in situ and cervical cancer. The expression of L1 was detected in each group by ELISA and Immunohistochemistry. Results 46 out of 54 cases were HPV16 positive(85.2%). The reaction between antigen and antibody was lower as the aggravated of cervical damage in ELISA; the positive reaction appeared in the epithelium tissue in Immunohistochemistry. which effected by tissue`s damaged degree and differentiated states. Conclusion There are higher rate of HPV16 detection in women. L1 expressed in epithelium which affected by tissue`s differentiation. The expression of HPV16 L1 was lower as the damage aggravated. Detecting HPV L1 earlier in clinic can provide the direction for cervical cancer.

Key words: human papillomavirus, cervical carcinoma, L1 capsid protein, PCR, Immunohistochemistry

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