Basic & Clinical Medicine ›› 2010, Vol. 30 ›› Issue (9): 956-960.

• 研究论文 • Previous Articles     Next Articles

Peroxynitrite Increases the Expression of PDCD4 Gene in Human Cerebral Vascular Smooth Muscle Cells

Lin LI, Xiao-rui CHEN, Sha-sha SONG, Yu-lian JIAO, Chun-yan MA, Yuan-rong JU, Jian-feng LI   

  1. Provincial Hospital affiliated to Shandong University Provincial Hospital affiliated to Shandong University
  • Received:2009-11-09 Revised:2010-01-24 Online:2010-09-05 Published:2010-09-05
  • Contact: Jian-feng LI

Abstract: Objective This study was designed to investigate the change of PDCD4 gene expression in cerebral vascular smooth muscle cells (HCVSMCs) in response to peroxynitrite (ONOO-). Methods HCVSMCs were cultured in vitro and subjected to different concentrations of ONOO-. Cell viability was measured by MTT assay. The morphological changes were assessed by acridine orange staining as well as Ho33342/PI double staining to determine whether cell experienced apoptosis. The apoptotic rate was determined by flow cytometry. In particular, the expression of PDCD4 gene was examined by the means of RT-PCR and Western blot simultaneously. Results Direct exposure of HCVSMCs to ONOO- was able to block the cell proliferation, which was further revealed via the apoptotic pathway. Under the stress by ONOO-, the expression of PDCD4 gene was apparently increased at both mRNA and protein levels. Conclusions PDCD4 gene may play a pivotal role underlying the ONOO--induced apoptosis in HCVSMCs.

Key words: Peroxynitrite, PDCD4 gene, cerebral vascular smooth cells, Apoptosis

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