基础医学与临床 ›› 2024, Vol. 44 ›› Issue (2): 231-234.doi: 10.16352/j.issn.1001-6325.2024.02.0231

• 研究论文 • 上一篇    下一篇

改善昼夜节律紊乱减轻糖尿病大鼠心肌缺血/再灌注损伤

秦小英1, 刘慧1, 韩冲芳2*, 贺建东2   

  1. 1.山西医科大学 麻醉学院,山西 太原 030001;
    2.山西白求恩医院 麻醉科,山西 太原 030001
  • 收稿日期:2023-07-31 修回日期:2023-11-27 发布日期:2024-02-05
  • 通讯作者: *hanchongfang2003@foxmail.com
  • 基金资助:
    山西白求恩医院优秀青年启动基金(2019YJ11)

Improving circadian rhythm disturbance reduces myocardial ischemia-reperfusion injury in diabetic rats

QIN Xiaoying1, LIU Hui1, HAN Chongfang2*, HE Jiandong2   

  1. 1. College of Anesthesiology, Shanxi Medical University,Taiyuan 030001;
    2. Department of Anesthesiology, Shanxi Bethune Hospital,Taiyuan 030001,China
  • Received:2023-07-31 Revised:2023-11-27 Published:2024-02-05
  • Contact: *hanchongfang2003@foxmail.com

摘要: 目的 探讨间断补充睡眠能否减轻昼夜节律紊乱加重的糖尿病大鼠心肌缺血/再灌注损伤。方法 给大鼠腹腔注射1%链脲佐菌素(STZ)30 mg/kg及高脂高糖饮食构建糖尿病模型。造模成功的40只糖尿病大鼠,随机分为4组,每组10只:假手术组(Sham组)、缺血/再灌注组[I/R组,结扎左冠状动脉前降支(LDA)30 min,再灌注120 min]、昼夜节律紊乱组(Crd组,每日持续24 h光照并提供食物)、间断补充睡眠组(Dss组,夜间每光照3 h关闭1.5 h补充睡眠)。三苯基四氮唑(TTC)染色法计算心肌梗死体积;ELISA测定血清中肌酸激酶同工酶(CK-MB)活性、肌钙蛋白Ⅰ(cTnⅠ)含量;Western blot测定心肌生物钟基因Bmal1Rev-erbα的表达量。结果 与Sham组相比,I/R组心肌梗死体积明显增大,血清CK-MB活性和cTnⅠ含量明显升高,心肌组织Bmall表达下调,Rev-erbα上调(P<0.05);与I/R组相比,Crd组心肌梗死体积增大,血清CK-MB活性和cTnⅠ含量升高,心肌组织钟基因Bmal1表达下调、Rev-erbα表达上调(P<0.05);与Crd组相比,Dss组心肌梗死体积减小,血清cTnⅠ含量和CK-MB活性降低,钟基因Bmal1表达上调、Rev-erbα表达下调(P<0.05)。结论 间断补充睡眠可减轻由昼夜节律紊乱加重的糖尿病大鼠心肌缺血/再灌注损伤。

关键词: 生物钟基因, 昼夜节律紊乱, 糖尿病, 心肌缺血/再灌注

Abstract: Objective To investigate whether discontinuous sleep supplementation can reduce myocardial ischemia-reperfusion injury in diabetic rats aggravated by circadian rhythm disorder. Methods The rats were injected intra-peritoneal with 1% streptozotocin(STZ) 30 mg/kg combined with high-fat and high-glucose diet to replicate diabetic model. Forty diabetic rats were randomly divided into four groups with 10 in each: sham surgery group(Sham group),ischemia-reperfusion group(I/R group), in which the left anterior descending coronary artery(LDA) was ligated for thirty minutes and reperfusion for 2 h,circadian rhythm disorder group(Crd group,24 h daily light and food),discontinuous sleep supplementation group(Dss group,every 3 hours of illumination and 1.5 hours break at night). We analyzed the myocardial infarct size (by 2,3,5-triphenyltetrazolium chloride staining),determined serum creatine kinase-myoglobin(CK-MB) activity and cardiac troponin Ⅰ(cTnⅠ) concentrations; the expression level of BMAL1 and REV-ERBα was determined by Western blot. Results Compared to the sham group, the I/R group showed a significantly increased in myocardial infarct size, serum CK-MB activity and cTnⅠ concentration. The expression of the myocardial biological clock gene BMAL1 was down-regulated, while the expression of REV-ERBα was up-regulated(P<0.05).Compared to the I/R group, the Crd group showed a significantly increase in myocardial infarct size, serum CK-MB activity and cTnⅠ concentration. The expression of the myocardial biological clock gene BMAL1 was down-regulated, while the expression of REV-ERBα was up-regulated (P<0.05).Compared to the Crd group, Dss group showed a significantly decrease in the myocardial infarct size, serum cTn concentration and CK-MB activity.Furthermore,there was an increased protein expression of BMAL1 and a decrease of REV-ERBα(P<0.05). Conclusions Discontinuous sleep supplementation can reduce myocardial ischemia-reperfusion injury in diabetic rats aggravated by circadian rhythm disorder.

Key words: biological clock gene, circadian rhythm disorder, diabetes, myocardial ischemia-reperfusion

中图分类号: