Atorvastatin promotes the expression of bone morphology protein-7 and SnoN in the kidney of diabetic rats
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Objective To observe the effects of Atorvastatin on the expression of Bone morphology protein-7 and SnoN in the kidney of diabetic rats. Methods The rats were divided into normal control（NC） group，diabetic (DM) group and atorvastatin lavage treatment (AT) group randomly.After 16 weeks,the rats were sacrificed to measure biochemical parameters ,and to observe the changes of pathomorphology.Meanwhile,immunohistochemistry and Western blot were employed to examine protein expression of collagen-I（Col-I）,collagen-III（Col-III）,BMP-7,SnoN,E-cadherin,Desmin and collagen-IV（Col-IV）in the renal tissue.Results Compared with NC group, the levels of blood glucose(BG), triglyceride(TG), kidney index and the urine trace albumin/uric creatinine (MA/CR) were increased remarkably in DM group（P<0.05）. In AT group ,the levels of BG, TG, MA/CR were reduced（P<0.05）. The glomerular atrophy was observed in the DM group compared with the NC group, and the alleviated renal fibrosis was observed in AT group. Compare with NC group, the expression of BMP-7, SnoN, E-cadherin were significantly decreased in DM group, the expression of Desmin, ColI, ColIII and ColIV were a significantly increased in DM group（P<0.05）. In vivo，compare with DM group, Atorvastatin increase the expression of BMP-7, SnoN, E-cadherin the expression of Desmin，ColI,，ColIII and ColIV were a significantly decreased in AT group（P<0.05）. Conclusion Atorvastatin promotes the expression of BMP-7 and SnoN ,therefore protect the kidney of diabetic rats.