Abstract: Objective: To explore the influence on L428 cell lines morphology, growth inhibition rate, apoptosis rate, tumor invasion and metastasis of different doses of Bortezomib, and then illustrate the mechanism that how Bortezomib influence the tumor invasion and metastasis so that we can provide new thought and therapeutic target for the clinical treatment of lymphoma. Methods: Inverted microscope was used to observe L428 cell growth state after treating cells with 10 nM Bortezomib for 24 h; MTT was used to detect the L428 cell growth inhibition rate after treating cells with different Bortezomib concentration and time; TUNEL kit was used to detect the L428 cell apoptosis rate after treating cells with different Bortezomib concentration and time; Transwell was carried out to detect the invasion and metastasis of lymphoma influenced by different Bortezomib doses; Western blot was carried to detect the apoptosis proteins expression of Bcl-2 and Bax influenced by different Bortezomib doses. Results: Cells have appeard cliques, wire drawing and sparse phenomenon after treating with 10nM Bortezomib for 24h when compared with the blank control group; MTT data has indicated that as the Bortezomib concentration increased from 10nM to 50nM and the treating time increased from 12h to 36h, ?the cell growth inhibition rate showed a trend of rising (P<0.05); TUNEL data has indicated that as the Bortezomib concentration increased from 10nM to 50nM and the treating time increased from 24h to 72h, ?the cell apoptosis rate showed a trend of rising (P<0.05); Transwell has indicated that as the Bortezomib concentration increased from 10nM to 50nM, the?cell invasion and metastasis ability decreased gradually (P<0.05); ?The Bcl - 2 protein level showed a trend of concentration dependence downgrade (P<0.05) and Bax protein level showed a trend of concentration dependence upgrade (P<0.05) when treating cells with 10nM, 30nM, 50nM Bortezomib for 48h. Conclusions: Bortezomib can influence the growth state and the ability of invasion and metastasis of tumor cells by influencing the expression of apoptosis proteins, and the inhibition rate of tumor cells are concentration dependence?in a certain dosage range, the study has important reference value for clinical treatment of lymphoma.

Key words: Bortezomib, lymphoma, growth inhibition rate, apoptosis rate, invasion and metastasis ability