Abstract: Objective To determine the effect of DMB on bone formation in OVX mice via the differentiation of BMSCs to osteoblasts. Methods We isolated the BMSCs and identified them by flow cytometry. The mice were divided into three groups: control、OVX mice and OVX mice+DMB. DMB was given for 2 month to observe the bone formation. Results DMB induced BMSCs proliferation in dose- and time-dependent manner. Moreover, DMB promoted osteoblasts differentiation and inhibited adipocytes differentiation. DMB could also increase the rate of BV/TV, the trabecular number and thickness. Conclusion DMB promotes osteogenesis in OVX mice through osteoblasts differentiation of BMSCs.

Key words: DMB, BMSCs, OVX, bone formation, osteoblasts