Table of Content

    05 July 2016, Volume 36 Issue 7
    Self-assembling peptide K2I4K2 enhances the thermal-stability of Taq DNA polymerase in PCR system
    2016, 36(7):  879-885. 
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    Objective: To design a self-assembling peptide K2I4K2 as a new type of surfactant to stabilize Taq DNA polymerase, to explore the half-life of Taq DNA polymerase at high temperature and the influence on PCR amplification efficiency. Methods: Circular dichroism spectrum was used to detect the secondary structure of short peptide K2I4K2 in different physical and chemical condition. Atomic force microscope was used to detect the nanostructure characteristics formed by K2I4K2. UV-vis spectrum was used to examine the impact on the half-life of Taq enzyme stabilized by K2I4K2 at high temperature. Ordinary PCR was used to verify the feasibility of the application of K2I4K2 in the PCR. RT-qPCR was used to detecte of K2I4K2’s effect on the amplification efficiency of Taq polymerase. Results: Peptide K2I4K2 can form nanofiber structure in salt solution, and owns stable secondary structure at high temperature. UV-vis spectrum result indicated that peptide K2I4K2 increases the half-life by 1.6-fold compared with the stability in buffer alone. Furthermore, RT-qPCR indicated that peptide K2I4K2 greatly increase the amplification efficiency of Taq polymerase. Conclusion: Peptide K2I4K2 more significantly increased the thermal stability of the Taq DNA polymerase at a high temperature, and can be well used in the PCR system.
    Endoplasmic reticulum stress is involved in bisphenol A-induced hepatic lipid deposition of mice
    2016, 36(7):  886-890. 
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    Objective To investigate the role of endoplasmic reticulum stress(ERS)in hepatic lipid deposition induced by bisphenol A(BPA). Methods Male CD1 mice were randomly divided into two groups:control group (n = 10)and BPA group(n = 10). Mice fed with BPA [500μg/(kg?d)] in BPA group for 8 weeks. The content of hepatic triglyceride(TG)was measured by enzymic assay kit and oil red O staining was used to determine the hepatic lipid accumulation. The mRNA levels of fatty acid synthase(FAS)and fatty acid transport protein 1(FATP1)were determined by RT-PCR. Western blot was used to detect the protein expression levels of ERS associated proteins(Bip、p-IRE1α/IRE1α、p-PERK/PERK、p-eIF2α/eIF2α and SREBP-1c). Results Compared with control group ,the level of TG was higher in BPA group(P < 0.05). Oil red O staining also revealed that BPA promoted hepatic lipid accumulation. The mRNA levels of FAS and FATP1 were higher in BPA group than those in control group(P < 0.05). The protein expression levels of Bip、p-IRE1α/IRE1α、p-PERK/PERK、p-eIF2α/eIF2α and SREBP-1c in BPA group were also significantly higher than control group (P < 0.05). Conclusion ERS could be involved in hepatic lipid deposition of mice induced by BPA.
    Effects of SEMA3G overexpression mediated by lentivirus on human pancreatic cancer cell line PANC-1
    2016, 36(7):  891-895. 
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    Objective To investigate the effect of SEMA3G protein overexpression on proliferation, invasion and migration of human pancreatic cancer PANC-1 cells in vitro. Methods PANC-1 cells of human pancreatic cancer were transfected with lentiviral vector carrying SEMA3G. The expression of SEMA3G at mRNA and protein level in celsl were detected by real-time PCR and western blot, respectively. CCK8 assay was applied to examine cell proliferation, the cell invasion was analyzed by transwell assay and wound healing assay was used to examine cell migration in PANC-1 cells. Results The results showed that stable transfected SEMA3G cell line of pancreatic cancer line was established successfully. The proliferation capacity of SEMA3G group was significantly lower compared to blank and the negative control groups (P<0.05). Similarly, cell invasion and migration capacity of SEMA3G group were significantly lower than the two kinds of control groups(P<0.05). Conclusions SEMA3G lentiviral vectors can effectively increase SEMA3G protein expression in PANC-1 cells, thereby inhibiting cell proliferation, invasion and migration.
    Naringenin ameliorates kidney injury by inhibitiing TGF-β1/smad signaling pathway in diabetic nephropathy rats
    2016, 36(7):  896-901. 
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    Objective To explore the protectory effects of naringenin (NAR) on kidney as well as its effects on TGF-β1/smad signaling pathway in diabetic nephropathy rats. Methods Experimental rats were randomly assigned into diabetic nephropathy group and naringenin group. After rats were treated with naringenin for 6 weeks, the 24 h-urinary protein (24 h-UP), Creatinine clearance rate (Ccr) and kidney index (KI) were detected. Glomerular pathological changes and glomerular area(GA) were observed by HE-straning. Type Ⅳ collagen(Col4)、fibronectin(FN) were detected by real-time PCR and Western blot. TGF-β1,smad7,t-smad2 and p-smad2 were detected by Western blot. Results Compared with CON group, the 24 h-UP (P <0.01), KI (P <0.01) and GA (P <0.05) were increased, whereas Ccr (P <0.01) was declined in DN group, the mRNA and protein of Col4 and FN were increased, the protein of TGF-β1 and p-smad2 were increased and smad7 was decreased. Naringenin treatment could significantly attenuated the renal lesion, inhibited the expression of Col4,FN,TGF-β1 and p-smad2, improved the expression of smad7. Conclusion Naringenin ameliorates renal function, has marked protectory effects on kidneys in diabetic nephropathy, possibly through inhibited the activation of TGF-β1/smad and reduced the deposition of ECM proteins.
    Rosiglitazone attenuates LPS-induced mice acute lung injury by reducing polarization of Th17 cells
    2016, 36(7):  902-906. 
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    Objective To investigate the effect of rosiglitazone(RSG) on acute lung injury (ALI) murine model and to explore the possible mechanism. Methods BLAB/c (n=24) mice were randomized into control group, ALI model group, RSG group and GW9662 group. The percentages of the inflammation cells in the bronchoalveolar lavage fluid (BALF) and the histopathological changes of the lung tissues were examined to reflex the degree of inflammation. Meanwhile, the levels of inflammatory cytokines were measured by ELISA. The expression of RORγt and PPARγ at mRNA and protein levels in the lung tissues was determined by RT-qPCR and Western blotting respectively. Results Compared with control group, the percentage of neutrophils and the contents of the inflammatory cytokines in LPS group were significantly increased in BALF(P<0.05). Meanwhile, elevated mRNA and protein levels of RORγt suggested increased number of Th17 cells. Compared with LPS group, the degree of lung inflammation decreased in RSG group accompanied with low expression of RORγt and high expression of PPARγ(P<0.05). GW9662 significantly antagonized the protective effect of rosiglitazone, showing obvious mouse lung tissue inflammation ,and higher mRNA and protein levels of RORγt as compared with RSG group(P<0.05). Conclusions Rosiglitazone attenuates lung inflammation by regulating the polarization of Th17 cells in a murine ALI model induced by LPS.
    Expression of tRNA-derived fragments in myocardium
    2016, 36(7):  907-911. 
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    Objective Study whether the transfer RNA (tRNA) derived fragments (tRFs) was expression in myocardium. Methods The anterior wall of left ventricle tissues which are matched to infarct zone and border zone were isolated and extracted the total RNA. The small RNA sequencing was conducted by the illumina sequencer. The sequences and dosage of expression were acquired. The software of Fastax and Bowtie, and perl language program were applied to annotate small RNAs with length between 30 and 36nt and compare the difference between the two area cardiac tissues. Results The tRFs were found to be expressed in the anterior wall of left ventricle tissues which are matched to infarct zone and border zone. In addition, it was found that the percentage of tRFs in matched to infarct zone and border zone was 9.15% and 7.30% comparing with total small RNA. The identified 28 tRFs and their expression are similar between the two areas. The percentage of two tRFs, GlyGC and ValCAC, were 84.73%和84.66% between the two areas. The expression difference between each tRFs in two areas was less than 4%. Conclusions tRFs were expressed in myocardium. The expressional profile of tRFs in the myocardial tissues which are matched to infarct zone and border zone in myocardium was similar.
    Expression of MYH9 in human hepatocellular carcinomatissue and its effects onproliferation and apoptosis in hepatocellular carcinoma cell line
    2016, 36(7):  912-917. 
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    Objective To analyze the expression of nonmuscle myosin heavy chain9 (MYH9) in thehuman hepatocellular carcinoma(HCC) and investigate the effects of silencingMYH9gene on the proliferation and apoptosis ability of HCC cell line SMMC-7721. MethodsImmunohistochemistry and western blot were used to analyze the expression of MYH9 proteinin 50 cases of HCC tissues and paraneoplastic tissues in HCC tissues and cancer-adjacent tissues, and the relationship between MYH9 and clinical pathological parameters was studied. Western blot was also used to analyze the expression of MYH9 protein in HCC cell lines SMMC-7721,HepG2 and normal liver cell LO2.RNA interference targeted silence MYH9 gene was transfected into SMMC-7721 cells. The proliferation and apoptosis of the transfected cells were assessed by CCK-8 assay and flow cytometry.ResultsThe expression of MYH9 in HCC tissues was found to be significantly higher than that of cancer-adjacent tissues(P<0.05),and the relative amount of MYH9 protein in SMMC-7721 and HepG2 was significantly higher than that in LO2(P<0.05). It was showed that downregulation of MYH9 inhibited cell proliferation(P<0.05) and induced the apoptosis(P<0.05). ConclusionThe expression of MYH9 protein in HCC tissues was significantly higher than that in cancer-adjacent tissues, and RNAi ofMYH9 in the SMMC-7721 cells may inhibit cells proliferation and induce apoptosis.
    Cadmium promotes proliferation of thyroid cancer cell line WRO through GPER1-ERK/AKT pathway
    2016, 36(7):  918-923. 
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    ObjectiveTo investigate the proliferative effect of Cadmium on thyroid cancer cell WRO and the involvement of GPER1 in this process.Methods Expression of GPER1 in MCF-7, MDA-MB-231 and WRO cell lineswere detected by Western blot; Three cell lines were divided into 7 groups, respectively: control, Cd (250, 500, 750 and 1000 nmol/L), E2 (10 nmol/L) and G1 (10 nmol/L) groups, Cell proliferationwere measured by MTT method; WRO cell were divided into groups: 1) control, Cd (5, 10, 15 and 30 min), E2 (15 min) and G1 (15 min) groups; 2) control, G15, Cd+G15, E2 and E2+G15 groups; 3) control, Cd, Cd+scramble siRNA and Cd+GPER1 siRNA groups; expression of p-ERK, t-ERK, p-AKT or AKT were detected by Western blot under the above three situations; WRO cell were divided into 6 groups: control, Cd, Cd+G15, Cd+PD98095, Cd+LY294002and Cd+GPER1 siRNA groups, cell proliferation was measured by MTT method. Results Thyroid cancer cell WRO expresses GPER1. Cd promoted cell proliferation in low concentrations (P<0.05), 500nmol/L Cd exhibit the most effective proliferative effect. Cd induced rapid phosphorylation of ERK and AKT with time (P<0.05), which peaked at 15min. Cd-induced ERK and AKT phosphorylationwas inhibited (P<0.05) either by inhibitor for GPER1 or siRNA target GPER1.Cd-induced cell proliferationwas suppressed (P<0.05) byG15,LY294002,PD98059 and GPER1 siRNA. ConclusionsGPER1-ERK/AKT signaling pathway was involved in Cd-induced proliferation of GPER1-positive thyroid cancer cell WRO.
    Combined gene vector can transduce rat mesenchymal stem cells efficiently and safely
    2016, 36(7):  924-928. 
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    Objective To explore the transgene efficiency and safety of combined vector of spermine-pullulan polymer (SP) and adenovirus vector (Adv) on rat bone marrow derived MSCs (MSCs). Methods To combine vectors, Adv was incubated with the SP at room temperature for 15 min to form SP-Adv, and the morphology was observed under a transmission electron microscope. Luciferase activity reflecting the transduction efficiency was determined by using a luciferase assay system, and cell viability was determined using an MTT assay system. The osteogenic differentiation was detected by alkaline-phosphatase staining, and adipogenic differentiation was detected by Oil-Red-O staining. Results We observed that Adv was coated with SP polymer and the size of SP-Adv was bigger than uncoated Adv. SP-Adv showed significantly higher transgene expression than uncoated Adv and did not affect cell viability of MSCs. SP-Advs did not impair the osteogenic nor adipogenic differentiation of MSCs. Conclusion SP-Adv can transduce mesenchymal stem cells efficiently, and their safety was demonstrated in cell level.
    Effect of miR-150 on the expression of migration related protein in MSCs from diabetic nephropathy mice
    2016, 36(7):  929-933. 
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    Objective To study whether miR-150 can regμLate the expression of CXCR4 in mesenchymal stem cells of diabetic nephropathy. Method Flow cytometry was used to evaluate the CD phenotype on the surface of MSCs. Adipogenic, osteogenic differentiation of MSCs were detected by fluorescence microscopy, real-time RT -PCR was used to detect the expression of miR-150 in MSCs. Also, the expressions of CXCR4, and SDF-1 were tested by Western blot. Transwell migration experiment was perform to detected MSCs migration Results : MSCs expressed positively the CD29(97.6%±2.2%), CD90 (99.1%±0.6%),and negatively the CD45(17.3%±4.5%). MSCs had the potency of differentiating into fat, bone cells. The expression of miR-150 in MSCs was reduced in DN compared with normal group, However, the expressions of CXCR4 and SDF-1 were declined in miR-150 over-expressed MSCs. Moreover,the numbers of MSCs migration were increased in miR-150 inhibitor compared with control group. Conclusions:The change of miR-150 in MSCs from DN may be an important factor to migration related protein expression.
    Small molecule inhibitor JQ1 suppresses growth and metastasis of mouse breast cancer
    2016, 36(7):  934-940. 
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    ObjectiveTo explore the effect of Fra1 inhibitor JQ1 on TAMs phenotype and growth and metastasis of breast cancer. MethodsJQ1 toxicity to mouse macrophages (RAW 264.7) was detected by CCK8. Fra1 protein expression inhibited by JQ1 was detected by Western blot, qRT-PCR and flow cytometry were performed to check the expression of M1and M2 markers in TAMs. Transwell test was used to detect migration of 4T1 cells affected by RAW264.7. In vivo, 4T1 cells transplanted mice were used to test therapeuticeffect of JQ1 combined with Taxol. ResultsJQ1 inhibits Fra1 protein expression and M2 phenotype in RAW264.7; Fra-1 inhibition in macrophages can decrease migration of 4T1 cells. JQ1, combined with Taxol, inhibits tumor growth and lung metastasis of mouse breast cancer. ConclusionsDownregulation of Fra1in macrophages by JQ1 can suppress M2 phenotype and migration of 4T1 cells, and inhibit tumor growth and lung metastasis in mouse breast cancer model.
    Identification of serological autoantibody for multiple sclerosis using proteome array
    2016, 36(7):  941-945. 
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    Objective To identify some new autoantibodies, as the assistant serum diagnostic indicator for multiple sclerosis (MS)Methods The experimental samples were consisted of two groups: MS patients group and health control(HC) group, each group contains 15 samples. For proteome array detection, both groups were separated into 3 smaller groups randomly and the sera of each smaller group would be mixed sufficiently before using. Results After comparing with HC group,have identified 27 kinds of autoantibodies, among which the NOL3, SLC16A4 and DTX1targeted autoantibodies have a higher positive rate. The total of autoantibodiesdetected from MS group are much more than HC group. The functional cluster analysis indicated that the autoantibodies are mainly targeting the neuron associated proteins.Conclusions The study indicated that the high-throughput proteome array is highly suitable for the discovering of autoantibodies in serum. The result has certain guidance over our further research.
    Deficiency of FcεR1 aggravates NAFLD in mice
    2016, 36(7):  946-950. 
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    Objective To study the role of IgE high affinity receptor FcεR1 on HFD-induced non-alcoholic fatty liver disease (NAFLD) in mice. Methods To induce NAFLD, both WT and FcεR1-/- mice from each group (n=10, male) were fed with a high fat western diet for 12 weeks. Body weight was measured at a fixed time every week. All mice were sacrificed after 12 weeks treatment. Serum and liver were harvested to examine liver relevant parameters. Results Compared with WT mice, body weight and liver/body ratio in FcεR1-/- mice were increased (P<0.05). Meanwhile, serum AST and ALT, blood lipids (including TG, CHOL), hepatic TG and hepatic Collagen III mRNA levels were also significantly elevated in FcεR1-/- mice(P<0.01), indicating a more severe NAFLD symptom. Conclusions IgE high affinity receptor FcεR1 may have a protective effect on HFD-induced NAFLD,the mechanism may be reducing body weight and improving lipid metabolism.
    Analysis of the interaction proteins of PIH1D1
    2016, 36(7):  951-955. 
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    Objective To investigate the binding proteins of PIH1D1. Methods A PIHID1 stable-expressed HEK293T cell strain was constructed using a FLAG-HA double-tagged PIHID1 expressing plasmid. After performing the FLAG-HA tandem affinity purification (TAP), the mass spectrometric was carried out to analyze the interaction protein complex of PIH1D. Results The FLAG-HA double-tagged PIHID stable-expressed HEK293T cell strain was obtained. We found a number of potential binding proteins of PIHID1 by means of the mass spectrometric, including RPAP3, UXT, PFD2 and PFD6 which were involved in RNAP II assemble factory and MONAD/WDR92 which was able to promote apoptosis. Besides we found PIP and CALM1 belonging to calmodulin-Ca2+ complex, and PKM and LCN1 which were classic enzyme of metabolism as binding proteins of PIHID1 for the first time. Conclusions PIH1D1 might participate in multiple biological processes including apoptosis, calmodulin-Ca(2+) signaling pathway, RNA polymerase II assembling, and metabolism regulation through interaction with a RPAP3,UXT,PFD2,PFD6,MONAD/WDR92,PIP,CALM1,PKM and LCN1.
    TRIM69 inhibits the expression of cleaved caspase7 and PARP in HeLa and HEK293T cell line with the treatment of UV and etoposide
    2016, 36(7):  956-961. 
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    Objective To identify whether human TRIM69 may inhibit the expression of cleaved capase7 and PARP in HeLa and HEK293T cells in response to UV irradiation or stimulation. Methods The gene of human TRIM69 was cloned into eukaryotic expression vectors,which was then transfected into HeLa or HEK293T cells.The cells were exposed to UV irradiation or stimulated with etoposide and then collected for western blot to detected the level of activation of caspase7 and the cleavage of PARP. Results The levels of expression of cleaved caspase7 and PARP showed a significant decline in the stable cells with ectopically expressed TRIM69 comparing with the control one (P<0.05). Conclusions Expressed TRIM69 inhibited the levels of expression of cleaved caspase7 and PARP in HeLa and HEK293T cells in response to both UV irradiation and etoposide stimulation.
    T-uc.33 located in the genomic loci of nPTB may function in the neural development
    2016, 36(7):  962-968. 
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    Objective Identification of T-uc.33, which is an ultra-conserved lncRNA locates in the genomic loci of nPTB, and exploration of its function during the neural development in mouse. Method Bioinformatics analysis was used to operate the data mining of 481 ultra-conserved regions (UCRs) and identified the T-uc.33 which located in the genomic loci of nPTB using experimental test; RACE was used to obtain the full length of T-uc.33; quantitative real-time PCR was performed to observe the expression pattern of T-uc.33 and nPTB during the developmental stages of mouse brain as well as the differentiation process of neural stem cell; Loss-of-function strategy was used to study the regulatory role of T-uc.33. Results A bona fide ultra-conserved lncRNA named T-uc.33 whose length was 807nt was screened and identified. The expression level of T-uc.33 was significantly positively correlated with nPTB and both of them shared the similar expression pattern during mouse brain development or the differentiation of NSC. Knocking down the expression of T-uc.33 could decrease the abundance of nPTB. Conclusion T-uc.33 is the ultra-conserved lncRNA which locates the genomic loci of nPTB and positively regulates the expression of it. T-uc.33 potentially plays the important role in mouse brain development and NSC differentiation.
    Forensic evaluation on a new 6 dye STR kit with 27 loci
    2016, 36(7):  969-975. 
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    Objective: To explore polymorphism and forensic application by detecting 27 STR locus simultaneously. Method : The DNA of the samples was extracted by magnetic beads, amplified with PowerPlex ? Fusion 6C amplification Kit with 6-dye fluorescences labeling, and analyzed with GeneMapper@ID-X software. The Population genetics parameters were evaluated with Power States v 1.2 software. In addition, The effect of system about trace and mixture sample was observed. Results: The twenty-three autosomal STR loci were of high polymorphism in Guangdong Han population.TDP was 1-2.30×10-28 ,TPE was 0.999 999 999. DYS391 and DYS570 DYS576 loci GD value was 0.481, 0.791and 0.751, respectively. Three Y - STR in 172 unrelated individuals male 58 kinds of haploid type was detected, haplotype polymorphism of 0.337. The trace and mixture samples STR detection rate is 93.33% and 96.67%. Conclusion: PowerPlex? Fusion 6C is a high genetic polymorphism system, which can be used for kinship analysis, identification of the individual, and database construction.
    Over-expressed Rab27B induces apoptosis and inhibits proliferation of MKN45 cells in vitro
    2016, 36(7):  976-980. 
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    Objective To explore the effects of Rab27B on the proliferation and apoptosis in human MKN-45 cells. Methods Constructed the Lv-Rab27B-EGFP carrier and transferred it to MKN-45 cells as Rab27B group. Set up blank and negative control group. Cell growth was measured by CCK8 assay, the cell cycle and apoptosis were detected by flow cytometry. Relative expression levels of Bax, Bcl- 2 and caspase3 were detected by Western blot. Results Compare to blank and negative control cells, the proliferation of Lv-Rab27B-EGFP MKN-45 cells were decreased(P<0.05). Flow cytometry showed that over-expressed Rab27B resulted in an increase of G0/G1 phase and apoptotic cells. Expression of pro-apoptotic protein Bax was increased, and that of anti-apoptotic protein Bcl-2 was decreased differentially(P<0.05), the expression of caspase3 also showed significant difference(P<0.05). Conclusions Rab27B over-expression can inhibit cell proliferation by regulating cell cycles and induce apoptosis mainly through regulating Bcl-2 and caspase family proteins expression in MKN-45 cells.
    Genetics characteristics of ear lobes onTujia Gejiaand Bai national populations in Guizhou province
    renguangxiang REN
    2016, 36(7):  981-983. 
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    ObjectiveTounderstand the population genetics of ear lobes of Tujia,Gejia and Bai populations in Guizhou province. MethodsA somatological survey on 1212 Tujia,Gejia ang Bai populations,The gene frequencies of ear lobes were calculation out according to the Hardy-Weinberg law.ResultsIncidence of earlobe were 47.97%,76.21%,67.98%.The frequency of dominant gene of ear lobes were 0.2787,0.5121,0.4341.and that of recessive gene were 0.7213,0.4879,0.5659 of Tujia,Gejia and Bai populations.b Conclusions The earlobe has specific genetic traits,The genetic traits of different ethnic groups. There are ethnic, gender and regional differences.
    ER stress induces apoptosis of human breast cancer cell line MDA-MB-231
    2016, 36(7):  984-989. 
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    Objective: To investigate endoplasmic reticulum(ER) stress- induced the apoptosis of estrogen receptor negative breast cancer cells; moreover, the effect of calpain2 on ER stress-induced the apoptosis is explored as well. Methods Experimental group(DMEM),control group(DMEM+DMSO), and experimental group, Tunicamycin (TM) of various concentrations acted on cells for different durations, MTT method and flow cytometry were used for detecting the cell proliferation inhibition rate and apoptosis rate; Western blot was used for the expression of the marker protein glucose regulated protein of ERS was determined, and based on the highest point of GRP78 expression, the drug concentration and timing of TM established by ERS were determined, determining the expressions of apoptosis proteins caspase4 and CHOP, as well as the expression of calpain and its endogenous inhibition enzyme calpastatin; ERS inhibitor (4-PBA) and calpain inhibitor (calpeptin) were respectively subject to pretreated cells,and the influences of the above effects observed. Results ERS featured inhibiting efficacies on the cells’ proliferations (9, 12, 18 μmol/L) were respectively (33.88±1.32)%, (51.51±8.85)% and (55.77±2.61)%,and in comparison with the controls (P<0.01); 9 and 12 μmol/L TM’s cell apoptosis rates induced by ERS were respectively (16.70±0.46)% and (28.10±1.09)%, in comparison to the controls (P<0.05); 9 μmol/L TM’s action on cells 24 h GRP78 yielded the highest expression and featured statistical significance in comparison to the controls (P<0.05), and those of 12 μmol/L were obviously down-regulated; ERS obviously up-regulated the expressions of caspase4, CHOP and calpain,and the expression of calpastatin down-regulated ( P<0.01~0.05). The effects can be reduced or blocked by 4-PBA and calpeptin (P<0.05). And the expressions of CHOP all down-regulated among them 4-PBA and the treated group( P<0.05). Conclusion: The strong ER stress can induce the apoptosis of breast cancer MDA-MB-231 cells; expect that, calpain2 may be engaged in its mechanism.
    Parameters analysis of peripheral red blood cell and urine of residents in Jinzhou hexavalent chromium polluted area
    2016, 36(7):  990-994. 
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    Objective To evaluate the residents’ status of peripheral red blood cell in hexavalent chromium areas. Methods 135 residents in Jinzhou were randomly selected nearby an alloy plant with 50 years of hexavalent chromium by cluster sampling method. We conducted a questionnaire-based survey and gather blood and urine sample.Results By using robust regression model adjusted for age, sex, smoking, drinking and BMI, we find association of chromium in urinary with RBC (β=-0.09,P<0.01),MCH(β=1.71,P<0.01) and MCV(β=0.29,P<0.05)respectively. Furtheranalysis showed thatMCV’s relation with urinarychromium limited to individualsyounger than 60.Theassociation with RBC was only seen forwomen and the association with MCHC was only seen for men. Conclusion Exposure to hexavalent chromium in the environment have chronical impact to peripheral red blood cell.
    Effect of Gli2 gene silencing on the angiopoiesis in hepatocellular carcinoma cell line SMMC-7721
    2016, 36(7):  995-999. 
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    Objective To explore the effect of Gli2 on the angiopoiesisin hepatpcellular carcinoma cell line SMMC-7721.Methods Complementary shRNA oligonucleotides targeting the Gli2 gene was designed and inserted into lentiviral vector.shRNA lentivirus were transfected intoSMMC-7721 cells and then fluorescence photographs were taken. The gene silencing efficiency was measured by qRT-PCR and Western blot. Interfered group,negative group and blank group were set up.Endothelial tube formation assay was used to detect the ability of angiopoiesis.Detection the expression of VEGF-A in supernant by ELISA;Meanwhile,the qRT-PCR、Western blot were used to examineVEGF-A and MMP-2 mRNA and protein expression. Results The transfection efficiency of shRNA lentivirus was about 90%.Compared with the negative group and blank group,the expression of Gli2in the interfered groupwas lower,the capacity of angiopoiesis was significantly weaken,the expression of VEGF-A in supernant was decreased.meanwhile,the expression of VEGF-Aand MMP-2 was declined significantly(p<0.05). Conclusion Silencing Gli2 gene could suppress angiopoiesis ability in hepatpcellular carcinoma cell line SMMC-7721.its mechanism may be related to the down-regulation of VEGF-A and MMP-2.
    Effects of different forced-air-warming strategies on incidence of shivering during cesarean section
    2016, 36(7):  1000-1003. 
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    Objective To study the effect of different forced-air-warming strategies on incidence of shivering during cesarean section. Methods Sixty patients scheduled for cesarean section were randomly divided into four groups: control group (group A), prewarming group (group B), combined warming group (group C) and intraoperative warming group (group D). In group A, conventional passive warming was applied. Active warming was applied only 15 minutes before the induction of anesthesia in group B, only during surgery in group D and both in group C. Core temperature, incidence of shivering and shivering grading status were recorded. Results Core body temperature in four groups were reduced over time and there was a significant lower change in group C(0.39±0.26℃)than the other three groups(P<0.01). The incidence of shivering in four groups were 60.0%, 13.3%, 6.7%, 6.7%, and was lower in group B, group C and group D than that in control group(P<0.01). Conclusions Forced-air-warming system could effectively reduce the incidence of shivering in patients with cesarean section, and prewarming combined with intraoperative warming could most effectively reduce intraoperative hypothermia.
    Genomewide association analysis of essential hypertension in Tibetan population
    2016, 36(7):  1004-1009. 
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    Objective To discover and identify genetic variants associated with hypertension through using genomewide association study (GWAS). Methods Using a approach which we called SNP-MaP (SNP microarrays and pooling), We conducted a GWAS to explore the association between multigenic polymorphisms and the development of EH in patients with essential hypertension and health controls in Tibetan population from Lhasa. In this study, DNA from patients or controls were pooled and genotyped using an affymetrix genechip array 6.0. After detected the SNP locus, the SNP frequencies were found in patients and controls respectively by sequencing or PCR-RFLP. The relationship between SNPs and EH was analyzed. Results 5 sequence tagged sites met the genomic criteria of P<9.2×10-8 after Bonferroni correction among whole-genome genetic markers. The differences of genotypes and alleles frequency distributions of the 5 SNP locus was detected by sequencing or PCR-RFLP. The results show that differences of rs17136827 and rs1866525 genotypes and alleles frequency distributions between essential hypertension and healthy controls were not statistically significant, and differences of rs 9865108, rs12541835 and rs 4547758 genotypes and alleles frequency distributions between essential hypertension and healthy controls were statistically significant in population. Conclusions Our results demonstrate that affymetrix human SNP microarrays and pooling provide a very effective platform for genome-wide analysis of SNPs which are susceptible to essential hypertension. The polymorphisms of SNPs(rs 9865108, rs12541835 and rs 4547758 ) are associated with essential hypertension in Tibetan population.
    miR-96 and metastasis suppressor 1 might be the biological markers of bone metastasis in prostate cancer
    2016, 36(7):  1010-1013. 
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    Objective To investigate the expression of the miR-96 and metastasis suppressor 1(MTSS1) in prostate cancer and their clinical significances. Methods Compaired with adjacent tissue, hybridization in situ and immunohistochemistry were used to detect the miR-96 and MTSS1 expression in collected prostate cancer tissue specimens from 89 cases patient. Results The positive expression rate of the miR-96 and MTSS1 were 87.64%, 16.85% in respectivly prostate cancer (P<0.01). The up-regulation of miR-96 expression was associated with Gleason score and advanced clinical stage(P<0.05), and the expression of miR-96 was higher with bone metastasis than without bone metastasis in prostate cancer(P<0.05). But the down-regulation of MTSS1 expression was associated with Gleason score and advanced clinical stage(P<0.05), the expression of MTSS1 was lower with bone metastasis than without bone metastasis in prostate cancer(P<0.01). The expression of miR-96 had a close negative correlation to that of MTSS1 in prostate cancer (P<0.01). Conclusions miR-96 and MTSS1 may be important biological markers of bone metastasis in prostate cancer.
    3D laparoscopic surgery for adrenal pheochromocytoma / paraganglioma:35 cases report
    2016, 36(7):  1014-1016. 
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    Abstract:Object To highlight the effectivity and safety of 3D-laparoscopic surgery to adrenal pheochromocytomas /paragangliomas. Methods 24 cases of adrenal pheochromocytomas and 11 paragangliomas proven pathologically from 2013 to 2015were treated by 3D-laparoscopic surgery. Endocrine secretion examinations , B-US , CT, MRI , 131IMIBG ,and octreotide were used to diagnose the diease. Patients took α-receptor blocker for 2-4 weeks preoperatively. 211 patients from 2003 to 2010 by 2D laparoscopic surgery were compared . Results All the operations were completed successfully. There were no major intraoperative complications. Mean tumor diameter in 3D group was (4.12±1.95)cm and (3.97±2.21)cm in 2D group , without significant difference (P>0.05). Mean estimated blood loss in 3D group was (57.8±35.3)ml and (84.6±56.3) ml in 2D group , without significant difference (P>0.05).Mean hospital stay after operation in 3D group was (3.9±1.3) d and (4.9±1.6) d in 2D group without significant difference (P>0.05) . Mean operating time in 3D group was (69±19)min and ( 97±29) min in 2D group, with significant difference (P <0.05).They were followed up for 3-30months. There were no recurrences of tumor .Conclusions 3D-laparoscopic surgery has obvious advantage in spatial location and the sense of depth for adrenal pheochromocytomas /paragangliomas ,which make the operation easier and shortens the operation time obviously.
    miRNA-related single nucleotide polymorphism could be biomarker in tumors
    2016, 36(7):  1017-1020. 
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    microRNA-related single nucleotide polymorphisms (miRNA-SNPs) could exert influences on tumor development, treatment efficacy and clinical outcomes. Thus, as biomarkers, miRNA-SNPs can be applied to evaluate the tumor susceptibility for individuals and play an predictive role in patients' clinical outcomes.
    Diabetic induced neuropathic pain and itch
    2016, 36(7):  1021-1025. 
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    Diabetic neuropathic pain (DNP) and itch are the well-known complications of diabetes. Despite much advances in diabetes complications, the exact mechanisms of diabetic neuropathic pain and itch are still not clear. In this review, we discuss various possible mechanisms of the pathogenesis of neuropathic pain and itch, including the role of hyperglycemia in altering the physiology of peripheral nerves and other mechanisms of diabetic neuropathic pain and itch.
    Researh advances of PAX6 gene in cancer
    2016, 36(7):  1026-1029. 
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    PAX6, which is a PAX gene family member, is a highly conserved transcriptional regulator, plays vital roles in multiple biological processes such as embryonic development and tumorigenesis. It was reported that PAX6 regulates target gene expression including cdc2, cyclin D1, p21, p27, MMP-2 and VEGFA, controls cell growth, apoptosis, cell cycle, invasion and angiogenesis, affects tumorigenesis and progression of various types of cancer. PAX6 will be a potential target in the field of cancer therapy.
    Circulating tumor DNA for gliomas: research progress
    2016, 36(7):  1030-1034. 
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    Circulating tumor DNA (ctDNA), known as an emerging molecular biological technique, plays an increasingly vital role in glioma’s early diagnosis, treatment susceptibility monitoring and individualized treatment plan formulation. The IDH and EGFRvIII mutations, methylation status of promoters of MGMT as well as loss of heterozygosity (LOH) in chromosomes arms 1p, 19q and 10q have become the hot spots of glioma research.