Basic & Clinical Medicine ›› 2016, Vol. 36 ›› Issue (7): 886-890.

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Endoplasmic reticulum stress is involved in bisphenol A-induced hepatic lipid deposition of mice

  

  • Received:2015-10-09 Revised:2015-12-02 Online:2016-07-05 Published:2016-06-22
  • Supported by:
    ;National Key Clinical Specialties Construction Program of China (No. [2013]544)

Abstract: Objective To investigate the role of endoplasmic reticulum stress(ERS)in hepatic lipid deposition induced by bisphenol A(BPA). Methods Male CD1 mice were randomly divided into two groups:control group (n = 10)and BPA group(n = 10). Mice fed with BPA [500μg/(kg?d)] in BPA group for 8 weeks. The content of hepatic triglyceride(TG)was measured by enzymic assay kit and oil red O staining was used to determine the hepatic lipid accumulation. The mRNA levels of fatty acid synthase(FAS)and fatty acid transport protein 1(FATP1)were determined by RT-PCR. Western blot was used to detect the protein expression levels of ERS associated proteins(Bip、p-IRE1α/IRE1α、p-PERK/PERK、p-eIF2α/eIF2α and SREBP-1c). Results Compared with control group ,the level of TG was higher in BPA group(P < 0.05). Oil red O staining also revealed that BPA promoted hepatic lipid accumulation. The mRNA levels of FAS and FATP1 were higher in BPA group than those in control group(P < 0.05). The protein expression levels of Bip、p-IRE1α/IRE1α、p-PERK/PERK、p-eIF2α/eIF2α and SREBP-1c in BPA group were also significantly higher than control group (P < 0.05). Conclusion ERS could be involved in hepatic lipid deposition of mice induced by BPA.

Key words: bisphenol A, lipid deposition, endoplasmic reticulum stress