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Table of Content

    05 April 2011, Volume 31 Issue 4
    TNF- ? activations of Nuclear Factor-κB activity in rats H9C2 cells
    GAO Xia MA Yi-tong YANG Yi-ning XIANG Yang CHEN Bang-dang LIU Fen DU Lei
    2011, 31(4):  351-354. 
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    Objective To evaluate the effect of tumor necrosis factor ? (TNF-?) on Nuclear Factor-κB(NF-κB) activity in cultured rats H9C2 cells. Methods H9C2 cells were cultured in vitro and treated with TNF-a , The quantitative analysis of the protein content and the DNA binding activity of NF-κB was examined by the Western blot method and electrophoretic mobility shift assay (EMSA) method. Alamar Blue assay was used to assess the proliferation of the treated cells. Results TNF-a could active NF-κB activation pathway in rats H9C2 cells,Alamar Blue assay reveal significant difference in the absorbance between the treated cells and the control cells. Conclusion Some regulating effect of TNF-? on rats H9C2 cells might be exerted through NF-κB activation pathway.
    microRNA-21 regulates the migration of Flk1+ mesenchymal stem cells
    WANG Shi-hua HUANG Shan Chun-jing BIAN, Chun-hua ZHAO
    2011, 31(4):  355-359. 
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    Objective To evaluate the effect of microRNA-21 on migration of Flk1+ mesenchymal stem cells (MSCs). Methods Flk1+MSCs were transfected with microRNA-21 inhibitor using Lipofectamin2000 and their migratory activity was evaluated by transwell. Taqman real-time PCR was applied to detect expression of microRNA. Results Compared to control group, the expression of microRNA-21 was significantly reduced in the inhibitor-transfected group. Moreover, the number of cells migrated through transwell in the inhibitor-transfected group was 77.5%, 71.3% and 69.8% of that in the control group at 12、16 and 20h , respectively. Conclusion microRNA-21 can regulate the migratory capacity of Flk1+MSCs.
    BMP9 inhibits invasion and migration of human MDA-MB-231 cells in vitro
    WANG Ke FENG Hong-lei SUN Xiao-xiao LUO Jin-yong ZHANG Yan
    2011, 31(4):  360-365. 
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    Objective To investigate the correlation amony BMP9 expression, migration and invasion of breast cancer MDA-MB-231 cells in vitro. Methods The expression of BMP9 in MDA-MB-231 was detected by RT- PCR, high titer of adenovirus vector expressing BMP9 was used to infect MDA-MB-231.Experimental group: MDA-MB-231/BMP9,control group: MDA-MB-231/GFP and MDA-MB-231.To detect the changes of invasion and migration in the recombinant MDA-MB-231/BMP9 cells colony-forming assay, cell wounding assay, Transwell invasion assay were carried out. Results Compared with MDA-MB-231/GFP cells and MDA-MB-231 cells, the mRNA level of BMP9 was increased in MDA-MB-231/BMP9 cells. the cell colony formation rate of experimental group was decreased from the control group (95.4 ± 3.1)% and (92.5 ± 2.4)% to (74.0 ± 3.6)% (P <0.05), the healing rate of experimental group was decreased from control group (95.4 ± 3.1)% and (92.5 ± 2.4)% to (74.0 ± 3.6)% (P <0.05) , the number of invading cells which moved across the matrix barrier for experimental group was decreased from control group (255.3±16.1) and (267.3±14.9) to(150.3±14.6)(P<0.05). Conclusion BMP9 can inhibit the invasion, and migration of breast cancer in vitro.
    ERK and P38 MAPK regulates the contractile response of smooth muscle in hypoxic rats through increasing MLC20 phosphorylation
    YANG Guang-ming LI Tao XU Jing TANG Jing LIU Liang-ming
    2011, 31(4):  366-370. 
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    Objective To observe the effect of ERK, P38 MAPK and JNK on the contractile response of vascular smooth muscle to norepinephrine (NE) after hypoxia, and explore its calcium sensitivity regulation mechanism of myosin light chain (MLC20) phosphorylation. Methods With hypoxia-treated vascular smooth muscle cell (VSMC) and hemorrhagic shock model of rats, the contractile response of VSMC to NE after hypoxia were observed by fluorometric method. At the same time, the MLC20 phosphorylation of superior mesenteric artery (SMA) in hemorrhagic shock rats was detected by Western blot, and the calcium sensitivityof SMA was assayed with isolated organ perfusion system. Results After hypoxia, the contractile response of VSMC to NE was significantly decreased. Meanwhile the level of MLC20 phosphorylation and calcium sensitivity of SMA after shock were decreased. Angiotensin II (AngII), with the effect of MAPK stimulation, could increase the contractile response of VSMC to NE after hypoxia, and increased the level of MLC20 phosphorylation and calcium sensitivity of SMA after shock. PD98059 (ERK inhibitor), SB203580 (P38 MAPK inhibitor) and SP600125 (JNK inhibitor) antagonized the effects of AngII-induced increase of the contractile response of VSMC after hypoxia. And ERK and P38 MAPK inhibitors also antagonized the effects of AngII-induced increased the level of MLC20 phosphorylation and calcium sensitivity of SMA after shock, while JNK inhibitor had no effect. Conclusions ERK, P38 MAPK and JNK were involved in the regulation of the contractile response of VSMC after hypoxia, and the mechanism of ERK and P38 MAPK was possibly through MLC20 phosphorylation dependent mechanism (calcium sensitivity regulation pathway), while JNK may be partially involved in regulating of the contractile response of VSMC by other mechanisms.
    Effects of airway epithelial cells transfected by endogeny polypeptide Elafin on human airway mucus hypersecretion
    Hong-mei YU Qi LI Juliy M Perelman Victor P Kolosov Xiang-dong ZHOU
    2011, 31(4):  371-376. 
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    Objective To explore the effects of transfection of endogeny polypeptide elafin on mucin (MUC)5AC overexpression induced by lipopolysaccharide. Methods Eukaryotic expression vector pEGFP-N1-Elafin was constructed and transfected into HBE16 Cells. Cells were then stimulated by lipopolysaccharide (LPS). Elafin was detected by immunofluorescence and observed by Confocal Laser Scanning Microscopy (CLSM), the protein level inhibitor of NF-κB (IκB)α was detected by western blot, the transcription activity of nuclear factor κB (NF-κB) was detected by luciferase reporter gene detection system, MUC5AC protein level was assessed by ELISA, the levels of Elafin mRNA and MUC5AC mRNA were detected by RT-PCR, respectively. Results The levels of Elafin protein and mRNA were obviously increased after transfection of recombinant Elafin into HBE16 cells, compared with LPS group or control group. There was an obvious increase of MUC5AC protein production and mRNA expression, with elevation of NF-κB activity, all significantly higher than control group, IκBα protein production was significantly lower than control group. Transfected recombinant elafin reduced MUC5AC protein and mRNA level, inhibited NF-κB activity, and increased IκBα protein production, compared with single LPS-stimulated group. Conclusion Transfection of endogeny polypeptide Elafin may down-regulate MUC5AC overexpression induced by LPS, and this is through the inhibition by Elafin of NF-κB activiation via effects on IκB pathway.
    Construction of HepG2-HBX cell line and validation of the silencing effect of siHBX
    YANG Bing CHEN Wei-xin WEN Yang-an HUANG Shi-feng ZHOU Qian-yun ZHANG Li-ping
    2011, 31(4):  377-382. 
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    Objective To construct the HepG2-HBX cell line that stably expressing the hepatitis B virus protein X (HBX) and to explore the specific silencing effect of siRNA on the HBX gene. Methods The pCDNA3.1(+)/HBX-Flag plasmid containing the HBX sequence was transfected into the HepG2 cell lines with lipofectamine 2000, and the HepG2-HBX cell line stably expressing the HBX gene was screened out with G418; RT- PCR and immunohistochemistry were employed to validate the expression of the HBX gene; the siHBX fragment targeting the HBX gene was chemically synthesized and transfected in to the HepG2-HBX cell line, and RT-PCR, real time quantitative RT-PCR and Western blotting were respectively performed to evaluate the the expression of HBX at both the mRNA and protein level in order to validate the specific silencing effect of siHBX on the HBX gene,moreover,the cell cycle of HBX19 was detemined by flow cytometry . Results: The HepG2-HBX cell line stably expressing the HBX gene was successfully constructed and the siHBX fragment could specifically inhibit HBX expression with high efficiency, moreover, it was shown to inhibit cell cycle progression. Conclusions: siHBX could be used as a targeted strategy to inhibit the HBX gene in liver cancer cells, laying an experimental basis for the subsequent therapeutic research in HBV infection-related liver cancers.
    Expression Reinforcement of Hedgehog Signaling Genes in Breast Cancer Stem Cells
    TAO Ya-jun MAO Jun ZHANG Qing-qing LI Lian-hong
    2011, 31(4):  383-387. 
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    Objective To explore the effect of Hedgehog signaling pathway in breast carcinogenesis. Methods CD44+CD24- cells and non-CD44+CD24-cells were selected from cell suspension in the culture system of a serum-free medium by magnetic activated cell sorting system (MACS), real-time RT-PCR was employed to detect the expression of SHH,PTCH1,SMO and GLI1 mRNA in CD44+CD24- cells and non-CD44+CD24-cells, immunohistochemistry test was applied to study the expressions of SHH,PTCH1,SMO and GLI1 protein in breast cancer. Results CD44+CD24- cells accounted for 8.25% of breast cancer suspension cells and showed the positive expression of ALDH1A1 protein and Oct-4 protein.The expression levels of SHH、PTCH1、SMO、GLI1mRNA in CD44+CD24- cells were higher than those in non-CD44+CD24-cells. SMO and GLI1 expressions were increased in triple-negative breast cancer.Conclusions Hedgehog signaling is activated in breast cancer stem cell CD44+CD24- cells and to stop the recurrence and drug resistance by inhibiting the activation of Hedgehog signaling in breast cancer cells maybe an effective measure.
    Epidermal growth factor upregulates voltage-gated sodium channel/Nav1.5 expression and human breast cancer cell invasion
    PAN Hui-yan ZHAO Li-hong DAI Yin-mei ZHANG Wei-hua CHEN Hong HUANG Bing-ren
    2011, 31(4):  388-393. 
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    Objective To investigate whether epidermal growth factor (EGF) regulates expression of voltage-gated sodium channel (VGSC) subtype of Nav1.5 as well as the possible intracellular pathway through which EGF may influences Nav1.5 expression. Methods Matrigel test, immunofluorescence, real-time fluorogentic quantitative PCR ( RFQ-PCR) and Western blot were used to investigate the level and intracellular distribution of EGF receptor (EGFR) , Nav1.5 protein, the effect of EGF on Nav1.5 mRNA expression and possible effect of PI3K on EGF enhancement of MDA-MB-231 cell invasion. Results MDA-MB-231 cells significantly expressed higher EGFR and Nav1.5 protein. EGF increased invasion of the cells approximately 51% ± 2.6% (P﹤0.05), and Tetrodotoxin (TTX) , the blocker of VGSC, suppressed the effect of EGF on the cell invasion (P﹤0.05) . The level of Nav1.5 mRNA and protein were increased by EGF treatment by 128 ± 4 times and 39% ± 4% (P﹤0.05 for both), respectively. Wortmannin , the inhibitor of PI3K, significantly reduced the EGF-induced increase of both Nav1.5mRNA and protein, and also decresed MDA-MB-231 cell invasion induced by EGF through Nav1.5. Conclusions EGF upregulated Nav1.5 mRNA and protein expression which promoted the invasion of strongly metastatic breast cancer MDA-MB-231 cells, and PI3K was found to be involved in the signaling cascade of EGF-induced VGSC/Nav1.5 expression.
    15d-PGJ2 attenuates CSE-induced inhibition of HUVECs migration
    2011, 31(4):  393-399. 
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    Objective To explore the roles of 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) in cigarette smoke extract (CSE)-induced change of human umbilical vein endothelial cells (HUVECs) migration. Methods To determine the migration and angiogenesis of HUVECs with“scratch assay” and capillary-like tube formation assay. To observe the expression of occludin with western blot. Results CSE significantly inhibits the migration of HUVECs. Cell number of wound closure in one visual field decreases from 629±28 in control to 364±17 in CSE group (P < 0.01). 15d-PGJ2 treatment increases the cell number of wound closure from 364±17 in CSE group to 546±20 in 15d-PGJ2+CSE group (P < 0.01). CSE inhibits the angiogenesis of HUVECs. 15d-PGJ2 could attenuate CSE-induced inhibition of HUVECs angiogenesis. The expression of occludin decreases from 0.37±0.04 in control to 0.12±0.03 in CSE group (P < 0.01) and increases to 0.28±0.04 in 15d-PGJ2+CSE group (P < 0.01 compared with CSE group), but still lower than control (P < 0.05). Conclusions CSE may inhibit the migration of HUVECs via down-regulation of occludin. 15d-PGJ2 could attenuate the change of occludin to rescue the HUVECs migration inhibited by CSE.
    Applying pulmonary function parameter changes to screen hypoxia-susceptible expeditioners of the Antarctic Dome-A at Icecap
    Fu-min HUANG Zheng-min GUO Bo QIU Shu-yu ZU Guang-jin ZHU Cheng-li XU
    2011, 31(4):  400-403. 
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    Objective We observed the changes of pulmonary function parameters at different altitudes, so as to explore the correlation between AMS(Acute mountain sickness), mood, and pulmonary function. This data may help predict hypoxia susceptibility to select qualified expeditioners of Antarctic Dome-A at Icecap. Methods Pulmonary function, AMS, and moods were examined in 35 pre-selected healthy male Chinese members of the 25th and 26th expeditions to the Antarctic Kunlun station who were being screened for hypoxia susceptibility in Lhasa and Yangbajing. Statistical analysis was performed with SPSS 15.0. Results There was an increase in levels of tidal volume (VT), breathing frequency (BF), minute ventilation (MV), maximum voluntary ventilation (MVV), forced expiratory volume in one second per forced vital capacity (FEV/1FVC), peak expiratory flow (PEF), 25% expiratory flow (FEF25%), 50% expiratory flow (FEF50%), and mid-expiratory flow (MMEF75/25%)(p<0.05). There was a decrease in levels of vital capacity (VC), forced vital capacity (FVC), and forced expiratory volume in one second (FEV1) in Lhasa and Yangbajing(p<0.05). According to AMSW scoring, we divided the team members into two groups, the AMS group and non-AMS group, and compared baseline pulmonary function parameters between them. Non-AMS group members had better baseline lung function than AMS group members. Conclusion The participants with better pulmonary function and psychological stability experienced a milder degree of AMS. Acute mountain sickness was positively correlated with SAS and negatively correlated with VC, FVC, FEV1, FEF25%, FEF50%, FEF75%, and MMEF75/25%.
    The immuno-modulatory defect of Cancer Stem Cell from Chronic Myeloid Leukemia patients
    Liang WANG Xue-bin QU Chun-hua ZHAO
    2011, 31(4):  404-408. 
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    Objective To investigate that whether the immuno-modulatory capacity is intact in CML patients bone marrow derived MSCs and what are the differences compare with those from normal donors. Methods Isolate MSCs from CML patients and normal donors respectively and exam their differences on the T lymphocyte proliferation by MLR, on the T lymphocyte cycle and apoptosis using flow cytometry. Results The results show that there is no obvious difference on the immunophenotype between MSCs from CML and normal donors, respectively. However, for the MSCs from CML patients, the capacity of suppressing T cell proliferation as well as G0/G1 phase (CML MSC:74.5%±1.2%;BMSC:94.0%±1.9%, p<0.05) is weakened, while the capacity of suppressing T cell apoptosis is enhanced (CML MSC:8.36%±1.31%; BMSC:14.1%±0.65%, p<0.05). Conclusion We propose that MSCs from pathological environment might be abnormal and should not be used for autologous transplantation.
    Melatonin inhibits IL-1β-induced monolayer permeability of human umbilical vein endothelial cells
    2011, 31(4):  409-413. 
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    Objective To determine the effect of melatonin (MLT) on interleukin-1β(IL-1β)-induced vascular permeability. Methods Human umbilical vein endothelial cells (HUVECs) were exposed to IL-1β with or without MLT at different concentrations, and endothelial cell permeability was assessed by measuring the flux of FITC-dextran across the HUVEC monolayer. To observe the distribution of vascular endothelial cadherin (VE-cadherin) using immunofluorescence staining analysis and determine the expression of VE-cadherin with western blot. Results Treatment with IL-1β(5, 10 and 20ng/ml) increased the relative permeability of HUVECs from 1.5±0.1 to 4.8±0.3, 5.2±0.4 and 9.8±0.8 (P < 0.01); MLT (10 and 100μmol/L) attenuated IL-1β (10ng/ml) induced permeability from 5.2±0.4 to 2.8±0.3 and 2.7±0.3 (P < 0.01). Melatonin improved IL-1β-induced disruption of VE-cadherin adherens junctions. The relative expression of VE-cadherin decreased from 0.91±0.04 in control group to 0.42±0.05 in IL-1β group (P < 0.01) and increased to 0.69±0.04 in MLT group (P < 0.01 compared with IL-1β group), but still lower than control (P < 0.05). Conclusions Our data indicate that MLT may strengthen the barrier integrity of HUVECs through attenuating the change of VE-cadherin.
    The clinical features of stroke in Mongolian family
    Hai-hua BAI Chang-chun QIU Buren-bayaer BAO Hui-guang WU Qi-zhu WU
    2011, 31(4):  414-416. 
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    Object: To explore the clinical specialty of stroke in Mongolian family .Methods: The pedigree was analyzed along with physical examination, stroke patients were been diagnose by CT or MRI, pick sample of blood 10ml to check the correlative physiological and biochemical criterion and gene research. Result:This family has 137 individuals, and including 10 strock patients 16 hypertension/ hyperlipoidemia patients, the result of blood fat diagnose indicated that the triglyceride’s and total cholesterol’s means of patients is higher than control group(p<0.05) ,and the HDL is lower than control group(p<0.05). Conclusion: The abnormity of blood fat and hypertension are main reason of the stroke.
    Hyperthermia-induced glioma invasiveness decreases is mediated by tumor necrosis factor-alpha
    2011, 31(4):  417-421. 
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    Objective Role of tumor necrosis factor-α(TNF-α) during hyperthermia- induced tumor invasiveness decreases process. Methods Immunohistochemical method detect proliferating cell nuclear antigen(PCNA) of glioma tissue treated by hyperthemia/TNF-α/normal saline(NS), radioimmunoassay monitoring TNF-α content of culture medium and brain glioma tissue after heat treated tumor-bearing rat and rat glioma cell line (C6 cell). Established a tumor model using Transwell, crystal violet staining determine tumor invasiveness. Electron microscope of tumor vascular endothelial cell apoptosis. Results Hyperthemia not only increased the content of TNF-α of C6 rat glioma cell culture medium and tumor tissue, but also decreased glioma invasiveness. Both reached the peak at 120 minute after hyperthermia(P<0.01). Hyperthemia and TNF-α used respectively on the tumor-bearing rats, all can caused apoptosis of tumor vasculature endothelial cells. TNF-α caused tumor vascular endothelial cell apoptosis levels had the same trend with TNF-α content of C6 cell culture fluid after hyperthemia. Conclusion Hyperthemia induced tumor vascular endothelial cell apoptosis and inhibit tumor invasiveness may be to increased TNF-α content in tumor tissue.
    The effect of PKC on human sperm motility and its mechanism
    Yu-qin DING Hong JIANG Cun-li WANG
    2011, 31(4):  422-425. 
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    Objective To explore the effect of PKC on sperm motility and its mechanism. Methods After standard semen analysis, 30 healthy volunteers (sperm density ≥20×106 /ml, a≥25% or a + b ≥50% ) and 20 infertile males with asthenospermia (sperm density ≥20×106 /ml, a<25% and a+b≤40%) were classified as control group and asthenospermia group,and the spermatozoa PKCβⅠin two groups were measured by flow cytometry(FCM). Moreover, spermatozoa in control group were incubated with PMA (PKC activator)for 15min,then treated with GF109203X(PKC inhibitor)for 15min, and the phosphorylation and protein expression level of ERK were detected by Western blot. Results The level of PKCβⅠin asthenospermia group decreased significantly compared with control group. After incubation with PMA, following the improvement of sperm motility, the phosphorylation level of ERK in spermatozoa increased significantly, however, after incubation with GF109203X, both sperm motility and phosphorylation level of ERK decreased significantly. Conclusions PKC may involve in the regulation of sperm motility through ERK signal transduction pathway,and the level of PKC in spermatozoa may play an important role in pathogenesis of asthenospermia.
    Uric Acid Inhibits the Synthesis of Nitric Oxide in Human Umbilical Vein Endothelial Cells
    Qi WANG Xue-jun ZENG Wei-gang FANG Lian-feng CHEN Lian HE
    2011, 31(4):  426-429. 
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    Objective To investigate the effect of uric acid (UA) on the expression of endothelial nitric oxide synthase (eNOS) and the production of nitric oxide in cultured human umbilical vein endothelial cells (HUVECs). Methods HUVECs were incubated with different concentration of UA (0、5、10、15、20mg/dl) and 50mg/L ox-LDL(as positive control) for 24h、48h and 72h.. Then, eNOS mRNA was detected by real-time PCR. The expression of eNOS protein was observed by western blot. NO in supernatant medium was analyzed by Enzymic method. Results UA 5mg/dl group could increase the expression of eNOS mRNA of HUVECs ,compared with placebo control group(P<0.05). While the concentration of UA increasing and time lasting, the expression of eNOS and the production of NO were decreasing significantly(72h NO2-/NO3- is 0.52±0.18 versus 1.00±0.1 for 2 mg/L versus control, P<0.05), the same with ox-LDL. Conclusion UA inhibited the expression of eNOS and the production of NO of HUVECs in a dose-dependent and time-dependent manner. It suggested that UA can damage the function of endothelial cells and may influence genesis and development of cardiovascular diseases.
    Over-expression of proto-oncogene Wip1 in intracranial ependymomas association with P53
    LIANG Chao-hui JIAO Bao-hua GUO Er-kun LU Sheng-kui
    2011, 31(4):  430-434. 
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    Objective To explore the expression of proto-oncogene Wip1 in intracranial ependymomas and its relationship with P53. Methods Thirty-one intracranial ependymomas and 10 normal brain tissues were detected for Wip1 mRNA and protein expression by the uses of immunohistochemistry, RT-PCR and western blot. Also, we detected P53 expression in 31 tumors through immunohistochemistry. Meanwhile, we reviewed patients’ clinical data and its relationship with Wip1. Results The positive expression of Wip1 protein in ependymomas(19/31) were higher than normal brain tissues(1/10) significantly(p<0.05). P53 positive staining were 2 in 31 tumor tissues. The values of Wip1 mRNA in ependymomas were 0.34±0.07, which higher remarkably than normal brain tissues(0.05±0.01)(p<0.05). The relative contents of Wip1 protein in ependymomas were 0.83±0.16,which higer remarkably than normal brain tissues (0.14±0.03)(p<0.05). The expression of Wip1 had nothing to do with tumor size,age and sex(p>0.05). Conclusions The high expression of Wip1 in ependymomas may regulate negatively the expression of P53. Wip1 may play an important role in the tumor progress development and would be a new target for ependymomas therapy in the near future.
    Evaluation of the clinical efficacy of CIK cell union chemptherapy for advanced gastric cancer after operation
    YAO Gen-dong HUO Hong-qi LI Peng LIU Ai-min WANG Hai-dong ZHANG Can
    2011, 31(4):  435-439. 
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    Objective Evaluate the clinical efficacy and secruity of CIK cell union chemotherapy for adanced gastric cancer after operation. Methods Choose 76 patients who suffered from adanced gastric cancer, divide them into CIK cell and chemotherapy union treatment group and the pure chemotherapy group. Patients with the blood culture and infusion processe are observed the security of CIK cell infusion, analyse and detect T lymphocyte subsets in vivo with FCM, compare its short-term clinical curative effect and their cancer-free survival time and overall survival time between the two groups with log - rank inspection. Results In the process of autologous CIK cell treatment, some cases appear the symtoms of slight fever, nausea, vomiting, not appear evident adverse reactions. CD4 + / CD8+ ratio increases after CIK cell infusion, NK expression increased (P < 0.05). In terms of short-term clinical curative effect, the objective effective ratio and the disease control ratio in chemotherapy union treatment group and CIK are hither than those in the pure chemotherapy group (41.03% vs 18.92%,61.54% vs 56.76%), (P < 0.05). The survival time of median cancer-free patients in pure chemotherapy group after operation are shorter than those of the union therapy group (P < 0.05). Patient’s postoperation median survival time was 36 months in pure chemotherapy group, Patients in union group, 39 months. Conclusion The technique of CIK cell and chemotherapy union therapy is effective,it can improve patient’s immunity, it can also make the patient's cancer-free survival time longer.
    Construction and identification of lentiviral over-expression system of human NSPc1 gene
    2011, 31(4):  440-444. 
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    Objective To construct and identify lentiviral over-expression system to study the function of NSPc1 using over-expression technique.Methods The cDNA sequence of human NSPc1 was chosen to design the primer,and restricted enzyme site were added to primer sequences.The DNA purified from PCR were digested with double restriction enzymes and then linked with linearized pLenti6-TO-EGFP-TRIP.The recombinants were identified by restriction digestion and DNA sequencing.The plasmids of positive clones were transfected into 293T cells, and Western blot was used to identify the over-expression efficiency of the lentiviral vector. Then 293T cells were used to package the lentiviral particles and infected by obtained lentiviral particles. The over-expression efficiency of NSPc1 lentiviral system was examined by Western blot. Results The human NSPc1 sequences was successfully inserted into pLenti6-TO-EGFP-TRIP vector,which was identified by double restriction digestion and DNA sequencing.Western blot validated that both the lentiviral vector and lentiviral particle can effectively over-express human NSPc1 gene in 293T cells. Conclusion The construction of over-expression lentiviral system of human NSPc1 gene makes the further study of function of NSPc1 gene possible using over-expression technique.
    Clinical analysis of two case reports of endobronchial lipoma
    Ruo-yun OUYANG Ping CHEN
    2011, 31(4):  445-446. 
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    Elevated serum levels of carbohydrate antigen 19-9 in two cases the elderly without neoplasm
    ZHU Li-qun YU Gui-lan
    2011, 31(4):  447-449. 
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    Dynamic state of serum CRP, TNF-α and interleukin-6 in severe multiple trauma patients
    YUAN Wei HAO Wei
    2011, 31(4):  450-451. 
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    The Myocardial Protective Effect of Modefied HTK Solution on the Isolated Rat Heart
    FU Hong-jie YUAN Ming GUO Wen-yi
    2011, 31(4):  452-453. 
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    Advance in the Research of microRNA103
    YU Dong-qi ZHOU Huai-jun
    2011, 31(4):  454-457. 
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    MicroRNAs (miRNAs) are a group of small noncoding RNAs of about 22 nucleotides in length, which mediate the post-transcriptional gene modulation by annealing to incomplete complementary sequences in the 3’untranslated regions of target mRNAs. miR-103 as an important member of microRNA family, plays a regulatory role in multiple tumors; it also has a potential role in non-tumor diseases.
    The functions of key transcription factors in the process of producing induced pluripotent stem cells
    LUO Hu LI Ze-gui
    2011, 31(4):  458-462. 
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    It has made great breakthrough in the filed of stem cell study recently that we can induce cell reprogramming to gain induced pluripotent stem(iPS) cell in vitro via adding transcription factors, such as OCT4 and SOX2 . Transcription factors (OCT4, SOX2 and Naong etc.) play an important role in starting the cell reprogramming, maintaining the pluripotency of iPS cells and deciding whether it differentiates. Researching the mechanism of action of these transcription factors is beneficial to the further explanation of the molecular mechanism of the cell reprogramming.
    Progress on JAK-STAT signaling pathway in cancer
    HONG Xuan ZHANG Yan-qiao
    2011, 31(4):  463-466. 
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    Numerous cytokines and receptors activate the Janus kinases and signal transducer and activator of transcription signaling, which has been recognized as one of the important pathways in human solid tumors and blood malignancies. JAK-STAT pathway affects the expression and function of a variety of genes that are critical to cell survival, proliferation, angiogenesis, and immune evasion. Recently, targeting JAK-STAT signaling pathway is the hot spot for cancer therapy and a variety of JAK-STAT inhibitors have been identified that induce antitumor cell effects in vitro and in vivo.
    Research progresses on growth factor receptor-bound 7
    ZHANG Dan GAO You-he
    2011, 31(4):  467-470. 
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    Growth factor receptor-bound 7(Grb7), a member of Grb7 protein family, has three distinct functional regions: a lot of proline amino acids exist in its N terminal, and the mesial district has such similar amino acids sequence as that of MIG-10 protein from Caenorhabditis elegans, and a SH2 domain in its C terminal. With these different domain, Grb7 protein plays distinct roles in cellular signaling pathway. And if we know more about the biological function acted by different domains of Grb7 protein, we will make advances in treating of patients with high expression of Grb7 protein.