Basic & Clinical Medicine ›› 2011, Vol. 31 ›› Issue (4): 351-354.
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GAO Xia 1,MA Yi-tong 1, YANG Yi-ning 2,XIANG Yang 2,CHEN Bang-dang 2,LIU Fen 2,DU Lei 2
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Abstract: Objective To evaluate the effect of tumor necrosis factor ? (TNF-?) on Nuclear Factor-κB(NF-κB) activity in cultured rats H9C2 cells. Methods H9C2 cells were cultured in vitro and treated with TNF-a , The quantitative analysis of the protein content and the DNA binding activity of NF-κB was examined by the Western blot method and electrophoretic mobility shift assay (EMSA) method. Alamar Blue assay was used to assess the proliferation of the treated cells. Results TNF-a could active NF-κB activation pathway in rats H9C2 cells,Alamar Blue assay reveal significant difference in the absorbance between the treated cells and the control cells. Conclusion Some regulating effect of TNF-? on rats H9C2 cells might be exerted through NF-κB activation pathway.
GAO Xia MA Yi-tong YANG Yi-ning XIANG Yang CHEN Bang-dang LIU Fen DU Lei. TNF- ? activations of Nuclear Factor-κB activity in rats H9C2 cells[J]. Basic & Clinical Medicine, 2011, 31(4): 351-354.
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http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2011/V31/I4/351