Abstract: Objective We observed the changes of pulmonary function parameters at different altitudes, so as to explore the correlation between AMS（Acute mountain sickness）, mood, and pulmonary function. This data may help predict hypoxia susceptibility to select qualified expeditioners of Antarctic Dome-A at Icecap. Methods Pulmonary function, AMS, and moods were examined in 35 pre-selected healthy male Chinese members of the 25th and 26th expeditions to the Antarctic Kunlun station who were being screened for hypoxia susceptibility in Lhasa and Yangbajing. Statistical analysis was performed with SPSS 15.0. Results There was an increase in levels of tidal volume (VT), breathing frequency (BF), minute ventilation (MV), maximum voluntary ventilation (MVV), forced expiratory volume in one second per forced vital capacity (FEV/1FVC), peak expiratory flow (PEF), 25% expiratory flow (FEF25%), 50% expiratory flow (FEF50%), and mid-expiratory flow (MMEF75/25%)（p<0.05）. There was a decrease in levels of vital capacity (VC), forced vital capacity (FVC), and forced expiratory volume in one second (FEV1) in Lhasa and Yangbajing（p<0.05）. According to AMSW scoring, we divided the team members into two groups, the AMS group and non-AMS group, and compared baseline pulmonary function parameters between them. Non-AMS group members had better baseline lung function than AMS group members. Conclusion The participants with better pulmonary function and psychological stability experienced a milder degree of AMS. Acute mountain sickness was positively correlated with SAS and negatively correlated with VC, FVC, FEV1, FEF25%, FEF50%, FEF75%, and MMEF75/25%.