Abstract: It has made great breakthrough in the filed of stem cell study recently that we can induce cell reprogramming to gain induced pluripotent stem(iPS) cell in vitro via adding transcription factors, such as OCT4 and SOX2 . Transcription factors (OCT4, SOX2 and Naong etc.) play an important role in starting the cell reprogramming, maintaining the pluripotency of iPS cells and deciding whether it differentiates. Researching the mechanism of action of these transcription factors is beneficial to the further explanation of the molecular mechanism of the cell reprogramming.