Abstract: Objective To explore the expression of proto-oncogene Wip1 in intracranial ependymomas and its relationship with P53. Methods Thirty-one intracranial ependymomas and 10 normal brain tissues were detected for Wip1 mRNA and protein expression by the uses of immunohistochemistry, RT-PCR and western blot. Also, we detected P53 expression in 31 tumors through immunohistochemistry. Meanwhile, we reviewed patients’ clinical data and its relationship with Wip1. Results The positive expression of Wip1 protein in ependymomas(19/31) were higher than normal brain tissues(1/10) significantly（p<0.05）. P53 positive staining were 2 in 31 tumor tissues. The values of Wip1 mRNA in ependymomas were 0.34±0.07, which higher remarkably than normal brain tissues（0.05±0.01）（p<0.05）. The relative contents of Wip1 protein in ependymomas were 0.83±0.16，which higer remarkably than normal brain tissues （0.14±0.03）（p<0.05）. The expression of Wip1 had nothing to do with tumor size，age and sex（p＞0.05）. Conclusions The high expression of Wip1 in ependymomas may regulate negatively the expression of P53. Wip1 may play an important role in the tumor progress development and would be a new target for ependymomas therapy in the near future.

Key words: ependymomas, Wip1