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Table of Content

    05 March 2011, Volume 31 Issue 3
    The coordinate alteration of actin cytoskeleton, CD44 and matrix metalloproteinase-2 in the metastasis of breast cancer cells
    ZHAO Wei HAN Hai-bo LIN Zhong-xiang ZHANG Zhi-qian
    2011, 31(3):  225-230. 
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    Objective To study the roles of actin and associated molecules in the control of human breast cancer cells malignant behaviors in vitro and in vivo. Methods A highly metastatic human breast cancer cell line BICR-H1 was compared with another breast cancer cell line MCF-7, which was well differentiated and non-metastatic. Western blot, immunofluorescence, gelatin zymography analysis and a chick embryonic chorioallantoic membrane (CAM) assay were used in this research. 5~30μg cisplatin or MMP-2 C terminal PEX domain were injected i.v. in CAM. Results BICR-H1 expressed high level of CD44, which was closely associated with actin aggregates at the bottom side of attached cells. It was also shown with MMP-2 activity. On the contrary, MCF-7 cells showed weak disruption of actin cytoskeleton structures and few actin aggregates. It expressed low or minimal level of CD44 and MMP-2. The expression of CD44 was down-regulated in cisplatin-treated BICR-H1 cells, and the activity of MMP-2 was also decreased upon PEX treatment. Both cell lines could form tumors in CAM, but only BICR-H1 cells could metastasize to distant tissues. Cisplatin inhibited the growth of BICR-H1 and MCF-7 cells in a time and dose dependent manner in CAM. The lung metastatic foci of BICR-H1 cells treated with 30μg cisplatin reduces from 30±15/embryo (PBS group) to 8±6/embryo, and the same dose of PEX can completely inhibits BICR-H1 metastasis. Conclusions It is concluded that actin cytoskeleton,CD44 and MMP-2 (ACM)molecular linkage is associated with breast cancer metastatic phenotypes, and that both cisplatin and PEX can interfere with the ACM molecular linkage, resulting in the suppression of both tumor growth and metastasis.
    Identification of Plasma membrane binding region of P57 with HeLa
    ZHANG Xiang JIN Meng-meng HUANG Li ZHNAG Shi-qian ZHANG Li-cheng TANG Fu-lin
    2011, 31(3):  231-236. 
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    Objective To study the property and function of p57 protein, finding the plasma membrane binding sites of p57. Methods The distributions of the wild-type p57 and its mutants in cells were observed by fluorescence microscopy. The blowing crack technology was used to detect the binding between the wild-type p57 and its mutants with plasma membrane. Differential centrifugation technology was used to prepare subcellular components and the contents of p57 in subcellular components were detected by Western blot. Results It was found that the fragment 386-461 of p57 has a similar distribution in cells and similar plasma membrane binding abilility as the intact protein, but the fragment 1-299 or 1-432 hasn’t. Overexpression of the fragment 386-461 can compete the membrane binding site with the intact p57. Conclusion The C-terminal domain containing the fragment 386-461 is responsible for the binding of p57 to plasma membrane.
    Relationship between mothers’ mitochondrial DNA A3243G mutation load and offspring’s
    MA Yi-nan FANG Fang CAO Yan-yan YANG Yan-ling ZHANG Ying WANG Song-tao QI Yu
    2011, 31(3):  237-241. 
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    Objective To study the relationship between mothers’ A3243G mutation load and their offspring’s. Methods 50 families carrying A3243G mutation were recruited and A3243G mutation load were analysis. Result (1) A3243G mutation load in mothers’ blood correlated positively with that in offspring’s blood, the correlation coefficient r=0.438,P<0.01;(2) A3243G mutation load in mothers’ blood correlated positively with that in offspring’s urine, the correlation coefficient r=0.355,P<0.05;(3) In the families with 2 offspring, mutation ratio was higher in mothers having two affected children. Conclusion A3243G mutation load in mothers’ blood may have a positive correlation with the mutation load in blood and urine in their children.
    Analysis of Left Ventricular Ejection Time and Associate Factors in Han and Korean-Chinese Ethnics of Heilongjiang
    PAN Yang-xing Bao-shen Qi Xiao-mei Zhou Shao-mei HAN, Guang-jin ZHU,
    2011, 31(3):  242-246. 
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    Objective: Analysis of left ventricular ejection time and correlated factors in Han and Korean-Chinese ethnics of Heilongjiang Provence. Methods: Left ventricular ejection time (LVET), accelerate index (ACI), pre-ejection period (PEP), stroke time ratio (STR) and other parameters were obtained by BIOZ Cardio Dynamics Monitor. Data were analyzed by SPSS 15.0 soft ware. Results: LVET in Korean-Chinese was shorter than in Han. According to standard coefficients, LVET was effected by stroke volume index (SI), STR, stroke volume (SV), ACI, PEP, systemic vascular resistance index(SVRI), AGE, body mass index(BMI), cardiac output(CO), systolic blood pressure(SBP), diastolic blood pressure(DBP), and gender from strong to weak respectively. The influence extent to the difference of LVET between Han and Korean-Chinese were STR(-20%), systemic vascular resistance(SVR)(-40%), SVRI(-20%), SBP(-20%), DBP (-20%), SV(-160%), SV+HR(-140%), SI+HR(-140%), CO+HR(-140%) and cardiac output index(CI)+HR(-140%) by single factor linear regression. Conclusion: Relative shorter LVET in Korean-Chinese may be result from their relative higher SVR and blood pressure and may be result in relative lower SV at meantime.
    NF-кB participate the process of sevoflurane preconditioning on myocardium against ischemia-reperfusion injury in rats
    QIN Qin XIE Hong WANG Chen LIU Xia ZHU Jiang WU Xue-mei
    2011, 31(3):  247-251. 
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    Objective To investigate the protective effects of NF-кB P65 active expression during sevoflurane preconditioning on rat myocardium against ischemia-reperfusion injury in vivo. Methods Seventy-eight male adult SD rats weighing 270-390g were anesthetized with intraperitoneal pentobarbital sodium 50mg/kg, tracheostomized and mechanically ventilated. PaCO2 was maintained at 25-40 mm Hg. Their chests were opened and hearts exposed. I/R was produced by reversible occlusion of left anterior descending branch (LAD) of coronary artery for 30 min followed by 2h reperfusion. Seventy-eight animals were randomly divided into 13 groups (n=6): (A) sham group;Rats received no ischemic-reperfusion. (B) Simple-Ischemic group;Rats were experienced myocardium ischemia-reperfusion merely. (C) Parthenolide (PTN) group;Nuclear Factor-κB (NF-κB) inhibitor PTN (500μg/kg) was administered intraperitoneally(IP). (D) Sevoflurane-I/R group;Rats received 2.5% sevoflurane for 30 min and 15 min wash-out followed by a 30 minutes occlusion and 2h reperfusion. (E) PTN+sevoflurane-I/R group;PTN administered IP 15 min before exposure to sevoflurane. (F) Sevoflurane-I/R+PTN group;PTN administered IP after sevoflurane preconditioning. Myocardium sample of all groups were collected before the time of myocardial ischemia and after the time of myocardial ischemia reperfusion respectively. In addition, (G) Sevoflurane group;Rats were received 2.5% sevoflurane for 30 min and then collected myocardium sample after 165 min. NF-κB P65 was determined by Western Blot analysis. Results Myocardium sample of all groups were collected before the time of myocardial ischemia: the expression of NF-κB P65 protein was significantly up-regulated in Sevoflurane-I/R group as compared to that of the sham group. Myocardium sample of all groups were collected after myocardial ischemia reperfusion: the expression of NF-κB P65 protein was significantly up-regulated in Simple-Ischemic group than that in the sham group and the expression of NF-κB P65 protein was significantly down-regulated in Sevoflurane-I/R group, sham group, SEVO group, PTN group, SEVO-I/R+PTN group and PTN+SEVO-I/R group as compared to that of Simple-Ischemic group. Conclusion Sevoflurane preconditioning has protective effects on ischemia-reperfusion myocardial injury in rats. This protective effect may be involved in the activation of NF-κB P65 during sevoflurane preconditioning.
    The protective effect of HDL on organs function of burn rats
    ZHENG Jian-sheng ZHENG Qing-yi
    2011, 31(3):  252-258. 
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    Objective To explore the protective effect of high density lipoprotein(HDL) on organs function of rats with severe burns. Methods One hundred and thirty-five Wistar rats were employed in the study and were randomly divided into control (n =15), burn (n=60, with 30%TBSA full-thickness burn on the back) and HDL[(n =60,with the injection of HDL (80mg/kg) via the caudal vein immediately after burns )]groups. The rats in the latter two groups were resuscitated with intraperitoneal isotonic saline. The serum content of ICAM-1 and TNF-αas well as the blood content of PCO2 and PO2 of the rats were determined at 12, 24, 48and 72 post-burn hours(PBH) and in control group. The pathological changes in the cardiac muscle, pulmonary tissue, hepatic tissue, renal tissue of the rats in all groups were observed at 12, 24, 48 and 72 PBH. Results PCO2 and the contents of ICAM-1, TNF-α, oxLDL, CK, ALT, AST, BUN, Cr in burn group were significantly higher, but the PO2 was lower than those in control group at each time-point (P<0.05). PCO2 and the contents of ICAM-1, TNF-α, oxLDL, CK, ALT, AST, BUN, Cr in HDL group were significantly lower, but the PO2 was higher than those in burn group at each time-point (P<0.05). Compared with the burn group, the pathological changes of the cardiac muscle, pulmonary tissue, hepatic tissue, renal tissue injury was markedly alleviated in the HDL group. Conclusion HDL has the protection effect on organs function of rats with severe burns via suppress inflammation reaction by reducing the expression of ICAM-1 and TNF-α.
    NEP1-40 promoted expression of Nogo A in phenylalanine treated neuron
    HUANG Cheng-jiao HUANG Li-su ZHANG Yong-jun YE Jun LI Duan GU Xue-fan
    2011, 31(3):  259-262. 
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    Objective Recently reasearch suggests that Nogo A expressed by oligodendrocytes and its receptor NgR may be involved in the etiology of neurophology of PKU or Hyperphenylalaninemia as an inhibitors, yet the action of Nogo A expressed by neuron is still not known. And whether its the specific antagonist to NgR (NEP1-40) can affect its expression. So the study was designed to investigate the effect by NEP1-40 on Nogo A in high phenyalanine(Phe) in vitro. Methods Cells was dissected from the cerebral cortex of E16~E18 embryos of SD rat, Real-time PCR and Western-Blot were used to evaluate the mRNA and protein of Nogo A respectively. The growth cones, axon and the Nogo A location were performed by immunofluorescence and immunohistochemistry respectively. Results Compared to the control, The mRNA and protein level of Nogo A with Phe for 12h, 24h and 48h were slightly reduced. NEP1-40 can upregulate expression of Nogo A on neuron in the high Phe condition with high level, but has no act on normal neuron. Conclusion NEP1-40 can promote expression of Nogo A on neuron in high Phe, which can help the neuron grow.
    Detection of two novel mutations in the tissue-nonspeci?c alkaline phosphatase(TNSALP)gene in a patient with hypophosphatasia
    2011, 31(3):  263-267. 
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    Objective In this study,the clinical and genetic characteristics of a Chinese boy with childhood hypophosphatasia was analyzed, and genetic mechanism and genotype-phenotype correlation discussed. Methods According to the clinical manifestation and laboratory findings, the preliminary diagnose of hypophosphatasia was given to the patient. Furthermore, genomic DNA was extracted from peripheral blood leukocytes of the patient, his family members and 50 ethnically matched, unrelated controls. All the 12 exons and ?anking intron sequences of the ALPL gene were ampli?ed by PCR. Direct DNA sequence analysis was performed by automated DNA sequencing. Results Sequence analysis of PCR products in the proband indicated that HPP originated from the heterozygous mutations c.18delA and c.G407C of the ALPL gene. The c.18delA mutation results in frameshift and premature termination of the translation. The predicted truncated protein (p.V7YfsX18) lacks almost all the crucial regions for the enzyme function and bone mineralization. The nucleotide transition G>C at position 407 resulted in an amino acid exchange from arginine 136 to praline. Both of the two mutations were not detected in 50 normal controls and not reported previously. After searching PubMed and the TNSALP gene mutations database, both of the two mutations were not reported previously. Pedigree analysis showed that the c.18delA and c.G407C had been inherited from the proband’s mother and father, respectively. In addition, his grandmother was also found carring the mutation c.G407C. According to the family pedigree, the disease was transmitted as an autosomal recessive trait. Conclusion The two novel mutations c.18delA and c.G407C in ALPL gene may provide new insights into the pathological mechanism and clinical manifestation of this rare disease.
    Neural induction of human umbilical cord mesenchymal stem cells
    CHENG Xin PI Ting XIAO Fei MA Liu-hong XU Jian-bing LUO Huan-min
    2011, 31(3):  268-274. 
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    Objective To investigate the potential of human umbilical cord mesenchymal stem cells(hUC-MSCs) differentiating into neural cells in vitro induction. Method Using three methods, including growth factor bFGF and EGF associated with RA, traditional Chinese medicine monomer ursolic acid (UA), the liquid supernatant of the neurons from cerebral cortex of newborn rat cultured in serum-free to induce hUC-MSCs to differentiate into neural cells, observe the morphological changes and detect the special markers of neural cells by immunocytochemistry. Result Induced by cytokines, UA (2 mg/l), hUC-MSCs could gradually become bipolar, multipolar or conical instead of fibrous, and the body may also shrink; and some could extend processes, elongating gradually with the time, even some sub-processes and network. The bodies of some other cells showed sphericity with short processes. Induced by the supernatant of the neurons from cerebral cortex of newborn rat cultured in serum-free media, hUC-MSCs may firstly differentiate into Nestin and Musashi-1 positive neurospheres, then undergo the morphological changes mentioned above and form neural cells. Shown by immunocytochemistry, a small quantity of hUC-MSCs express Tubulin, GFAP, or O4 after induction of 14 days. Conclusion hUC-MSCs can be induced in vitro to differentiate into neural cells under certain conditions, providing a new approach for cell transplantation treatment of nervous system diseases.
    Annexin A3 inhibits apoptosis induced by cisplatin in human epithelial ovarian cancer cells
    Jie YIN Yong-li ZHANG Xin YAO Xiao-yan WANG Ning MAO Ling-ya PAN
    2011, 31(3):  275-280. 
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    Objective To determine whether annexin A3 could regulate cisplatin sensitivity through apoptosis induced by cisplatin or not. Methods Retroviral vector constuction and cell infection technology were used to obtain high-level annexin A3 and low-level annexin A3 ovarian cancer cells. Then Annexin V/propidium iodide analysis and western blot were used to determine the cell apoptosis rate and caspase cleavage. Results After annexin A3 level was up-regualted, the average apoptosis rate induced by 60μg/ml cisplatin in sensitive cells was changed from 68.72%±1.01% to 29.13%±2.61% (P<0.05), cleavages of caspase and PARP were induced by high level cisplatin. Conversely, in cisplatin resistant cells with low-level annexin A3, the average apoptosis rate was up-reguated from 35.05%±3.06% to 76.73%±6.42% (P<0.05), low level cisplatin stimulated cleavages of caspase and PARP. Besides, annexin A3 could delay phosphorylation of p38 MAPK in ovarian cancer cells. Conclusion All these results showed that apoptosis inhibition might be a potential mechanism of annexin A3 regulating platinum resistance in ovarian cancer cells.
    Losartan suppress expression of lectin-like oxidized LDL receptor-1 in lung tissue of ALI rat
    2011, 31(3):  281-285. 
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    Objective To study the effect of losartan on expression of lectin-like oxidized LDL receptor-1 (LOX-1) protein in acute lung injury (ALI), to investigate its role in the process and mechanism of the effect of losartan on ALI. Methods Rats were randomly divided into normal control group, lipopolysaccharide (LPS) group (5mg/kg), and LPS+Losartan group, each group 10 rats. Rats in LPS+Losartan group were given an intraperitoneal injection of losartann (8mg/kg) after LPS administration. The level of PaO2, wet/dry ratio(W/D), the concentration of protein and TNF-α in bronchoalveolar lavage fluid (BALF), T?N?F-α concentration in plasma, lung MPO (myeloperoxidase) activity by chromometry and lung tissue histopathological changes were examined, LOX-1 mRNA was detected by RT-PCR analysis, the expression of LOX-1, ICAM-1, Cleaved caspase-3 protein in lung was measured by Western blot. Results Histological examination showed that extensive lung inflammation were seen in the LPS group. The level of PaO2 in LPS group decreased compared with taht in sham group (P<0.05). The level of W/D , concentration of protein in BALF, TNF-α in BALF and plasma, lung MPO activity, the expression of LOX-1 mRNA, LOX-1, ICAM-1 and Cleaved caspase-3 protein in LPS group were increased significantly respectively compared with those in sham group (P<0.05). Compared with LPS group these changes were markedly attenuated in LPS+Losartan group (P<0.05). Conclusion There is protective effect of losartan on ALI, which may result from inhibiting the inflammation and apoptosis induced by LOX-1.
    UCP1 and UCP3 play an important role in the early stage of the development of obesity in OLETF rats
    JIN Cheng-ji WANG Xiao-mei LI Xiang LI Xiang MU Yi-ming MU Yi-ming
    2011, 31(3):  286-290. 
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    Objective To investigate the relationship between the body weight change and the mRNA or protein expression of uncoupling proteins (UCPs) in OLETF rats. Methods Body weights were measured per weeks. UCP1 protein expression in the brown adipose tissue was detected by western blotting, while UCP2, UCP3 mRNA expression in the skeletal muscle was measured by northern hybridization in the OLETF and LETO rats at age of 7,10 and 25 weeks. Results OLETF rats gained weight at a faster rate than LETO rats, and the difference in the rate of weight gain was most prominent around 9 weeks of age. UCP1 protein level was 1.9-fold higher in LETO than OLETF rats at 10 weeks of age (p<0.05). UCP3 mRNA level was 5.5-fold higher in LETO than OLETF rats at 10 weeks of age (p<0.01). Conclusions: UCP1 and UCP3 expression may play an important role particularly in the early stage of the development of obesity in OLETF rats.
    Construction and Identification of SHH-N Gene Adeno-associated Virus Vector and Its Effection on Genes Related to Proliferation In Neural Stem Cells
    LIU Dong-sheng CUI_Yan SHEN Lun DU Yan-ping Gui-lin LI Ren-zhi WANG WANG Yan ZHANG Bo
    2011, 31(3):  291-296. 
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    Objective Construct and identify SHH-N gene adeno-associated virus vector and detect its effections on genes related to proliferation in neural stem cells(NSCs).Methods Isolated and cultured the neural stem cells in the subventricular zone of postnatal rat brain, SHH-N was gained by clone.pSNAV2.0-CMV-SHH-N-IRES-EGFP was established by using enzyme cutting and ligation , and then transfecting to packaging cell line 293T to acquire virus. Real time PCR was performed after SHH-N was infected neural stem cells by rAAV-SHH-N-EGFP vector for 48 hours, SHH-N gene expression of infected cells after 2 week was obversed by fluorescence microscope. Result (1)SHH-N gene was coincident with NCBI report.(2) pSNAV2.0-CMV-SHH-N-IRES-EGFP expression vector and rAAV-SHH-N-EGFP vector was successful established and packaged.(3) Real time PCR was performed after SHH-N infection for 48 hour, induction of Nmyc and Gli1 in rAAV-SHH-N-EGFP -treated group was enhanced compared to control group.Conclusion rAAV-SHH -N-EGFP vector had been successfully established and it can stably express in neural stem cells. Forced expression of SHH-N in NSCs resulted in enhanced induction of Nmyc and Gli1.
    The expressions and significance of apoptosis gene and protein of colon smooth muscle cell in diabetic colon dysmotility
    SUN Man-yi LIU Yan FENG Ping
    2011, 31(3):  297-302. 
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    Objective To explore the apoptosis gene and protein expressions of colon smooth muscle cell in diabetic colon dysmotility. Methods Thirty-six male Sprague-Dawley(SD) rats were randomly divided into 4 groups which included control 6w and 10w group、DM 6w and 10w group (n=9). Fasting blood glucose(FBG)、gastrointestinal transit rate and the serum level of fasting insulin (FINS) were detected, and the changes in the colon smooth muscle were examined by HE staining. The mRNA expression levels of BAX、BCL-2 and CASPASE-3 in the colon smooth muscle cells were determined by real-time quantitative PCR(RT-qPCR). Immunohistochemistry was used to detect the protein expression of CASPASE-3.Results ⑴ FBG of DM group was significantly higher than that of the control group. Gastrointestinal transit rate and FINS of DM group were significantly lower than those of the control group. The colon smooth muscle in DM group became thinner than that in the control group. The mRNA expression levels of BAX and CASPASE-3 in DM group were higher than those in the control group and the mRNA expression level of BCL-2 in DM group was lower than that in the control group. The protein expression of CASPASE-3 in DM group was higher than that in the control group(control 6w and 10w group are 4694±807、4711±812;DM 6w and10w are 6974±952、12474±967),( P<0.01).⑵ The changes above are more significant in DM 10w group compared with DM 6w group,(P<0.05). Conclusion Diabetic colon dysmotility may be closely associated with the changes of apoptosis gene and protein expressions of colon smooth muscle cell, and the apoptosis may become more significant with disease developing.
    S-adenosyl-L-methionine increase insulin sensitivity in OLETF rats
    JIN Cheng-ji WANG Xiao-mei LI Xiang FENG Hai-juan MU Yi-ming
    2011, 31(3):  303-307. 
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    Objective To investigate the effective of S-adenosyl-L-methionine (SAMe) on skeletal muscle mitochondrial DNA density and insulin sensitivity in Otsuka Long-Evans Tokushima Fatty (OLETF) rats. Methods SAMe (15 mg /kg?d ) or placebo was administered intraperitoneally for 6-week-old OLETF rats. Food intake, body weight and skeletal muscle mitochondrial DNA density at different age were determined. Insulin sensitivity was evaluated by euglycemic-hyperinsulinemic clamp test. Results SAMe treatment significantly inhibited food intake and reduced body weight gain as well as increased skeletal muscle mitochondrial DNA density and whole body insulin sensitivity in OLETF rats compared with their controls. Furthermore, skeletal muscle mitochondrial DNA density correlated with insulin body weight (?=0.696. p<0.05). Conclusion SAMe treatment increased mitochondrial DNA density in the skeletal muscle , prevented body weight gain, and improved whole body insulin sensitivity in OLETF rats.
    The elevated fasting palsma glucose levels is not conducive to postoperative recovery in patients with esophageal and cardiac cancer
    ZHENG Qing-feng Yao Zhen-dan Li Shao-lei Wu Nan Chen Jin-feng Zhang Li-jian YANG Yue
    2011, 31(3):  308-311. 
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    Objective To study the realation of elevated fasting plasma glucose levels after operation and postoperative recovery in patients with esophageal and cardiac cancer. Methods From January 1996 to October 2009, 196 cases of surgical treatment in the thoracic surgery II of Peking University School of Oncology were recruited, observing and recording fasting plasma glucose levels before and after operation and postoperative complications . χ2 test was used to analyze the data. Results (1) The patients with elevated fasting glucose levels after operation were significantly more than those before operation ,respectively 115 cases and 38 cases; (2) No significant correlation was found between elevated fasting plasma glucose levels in preoperative patients and postoperative complications; (3) In postoperative patients with elevated fasting glucose levels, the incidence of postoperative severe infection and anastomotic leakage was significantly higher. p values were 0.028 and 0.002. Conclusion he elevated fasting plasma glucose levels in the patients of esophageal and cardiac cancer after operation is not conducive to postoperative recovery .The effective control of postoperative fasting plasma glucose to normal levels may contribute to patients recovery.
    Effect of Treatment Tragedy and Response on Survival of Small Cell Lung Cancer-Multivariate Analysis
    SHAO Ya-juan NING Xiao-hong WANG Yu-zhou
    2011, 31(3):  312-315. 
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    Objective To explore the effect of different treatment and different response on the prognosis of small cell lung cancer. Method Collect patients diagnosed as small cell lung cancer and admitted to our department to receive chemotherapy between Jan 2002 to Jan 2010, review their records and do follow-up. Analyze the potential variables and only factors associated with survival with a P value less than o.1 in univariate analysis were introduced into the Cox regression model. Results 76 cases were included in this study. For local stage, 1-year,2-year, and 5-year survival rate are 81%,56%,21%,respectively. For extend stage,1-year,2-year survival rate are 59%,24%. Chemotherapy combined with radiotherapy, CR+PR obtained in first-line chemotherapy are associated with survival. For local stage, complete resection is associate with survival. In Cox regression, the variables above are all independent factors of survival. Conclusion Chemotherapy combined with radiotherapy, increasing the effectiveness of first-line regimen, and complete resection for selected local stage patient may be the way to improve survival of small cell lung cancer patients.
    PEP-1-CAT fusion protein preconditioning alleviated myocardial ischemia-reperfusion injury in rats in vivo
    ZHANG Yong-jun WANG Jia-ning TANG Jun-ming HUANG Yong-zhang YANG Jian-ye GUO Ling-yun ZHENG Fei
    2011, 31(3):  316-317. 
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    The effects of intensive insulin treatment palliate oxidative stress in type 2 diabetic rats
    WANG De-feng SUN Li LI Yan CHEN Xing-qi LIU Hong WANG You-ming
    2011, 31(3):  318-319. 
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    TGF?1 expression in cultured human lymphatic endothelial cells in vitro
    WANG Pei-liang
    2011, 31(3):  320-321. 
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    Correlation between between Serum IL-6, IL-8, Changes of NSE and Diagnosis of Head Injured Patients
    LI Xiao-li CHEN Ke-fu
    2011, 31(3):  322-323. 
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    Autophagy and Helicobacter pylori infection
    XIONG Li-jing TONG Yu MAO Meng
    2011, 31(3):  324-327. 
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    Helicobacter pylori is a microbial pathogen that causes erosions of gastric epithelial cells, via escaping immune killing in local, which leads to a sustaining infection, and it is associated with various gastric diseases. Autohagy is an evolutionary cell mechanism which not only eliminates intercellular microorganisms, but also has no influence to the survival of cells. Helicobacter pylori invade into gastric epithelial cells and certain immune cells, inducing the occurrence of autophagy, and are cleared off after replicating in the autophagsomes. It is assumed that autophagy might play an important role in Helicobacter pylori infection.
    Cyclic-AMP Response Binding Protein and apoptosis
    Guo XIONG Kang-hua MA
    2011, 31(3):  328-330. 
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    cAMP response element binding protein, which controls expression of target genes under the activation by various factors and plays an important role in the process of adjusting apoptosis, is the transcription factor located in the nucleus of eukaryotic cells。 Apoptosis implicates in the growth、homeostasis、defensive reaction,and so on.
    The Researching Progress of Th17 Cells Related Cytokines and Dermatosis
    ZHENG Wen MA Hui-qun
    2011, 31(3):  331-334. 
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    Th17 cells are a newly appreciated T-helper cell subset, which distinct from both Th1 and Th2 cells. The cytokines produced by Th17 cells include IL-17A,IL-17F,IL-21,IL-22,IL-6,TNF-α and so on. These cytokines can play an important role in the onset and development of dermatosis. Investigations about the relationship between Th17 cells related cytokines and dermatosis may illustrated more about the nosogenesis of dermatosis such as psoriasis and atopic dermatitis,and provide new target for their therapy.
    Calnexin modulates cellular apoptosis induced by endoplasmic reticulum stress
    2011, 31(3):  334-338. 
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    Endoplasmic reticulum stress(ERS) could induce apoptosis of mamy kinds of cell, that is relative closely with disease development. Calnexin, a kind of chaperon in endoplasmic reticulum, could modulate the apoptosis induced by ERS via several pathway invovling UPR, recruiting apoptosis-related factor, activating IRE1-JNK, and so on.
    Research about NCRR’s Strategy and Consideration in Developing Translational Medicine in China
    LI Ya-zi QIAN Qing WANG Min XU Pei-yang
    2011, 31(3):  339-343. 
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    To obtain much comprehension of the aim, research project and strategic plan of National Center for Research Resources(NCRR), strengthen the cooperation with NCRR and provide some suggestions on translational medicine study in China. By introducing NCRR, including its mission, funded projects, and the role in NIH’s roadmap, to perceive the gap between developed countries and china for the research and practice in translational medicine. Finally concludes the skill in researching translational medicine. Compared with some developed countries, China is still at an elementary stage in translational research. To promote translational medicine research in China, a series of measures should be adopted, such as making full use of the advanced theories, converting concepts, integrating relevant resources, etc.
    Advancement in the Research of Animal Models of Hyperuricemia
    LIU Shu-fen ZENG Xue-jun
    2011, 31(3):  344-347. 
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    The incidence of gouty and hyperuricemia has been increasing recently. It has been accepted that hyperuricemia is highly associated with cardiovascular disease. However the mechanism is still unclear. It is necessary for studying hyperuricemia to build a more suitable animal model. Although there are various methods of building hyperuricemia model, no one is accepted by all users.
    The Common Problems And Strategies In The Teaching Of Surgical Medical Records
    Lin ZHU Gui-xing QIU Jian-chun YU
    2011, 31(3):  348-350. 
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    Objective Evaluate the specialties of the surgery medical record and the common problems the medical students had in writing it, so as to accelerate the clinic teaching of the surgery department and increase the clinic skills of the students. Methods 912 cases of medical records written by the interns of PUMCH were summarized. The common problems and deficiency were evaluated. Combined with the specialties of surgical medical records, the methods and strategies in surgical medical records teaching are proposed. Results There were some common problems in the 912 cases of medical records. The medical history and case characteristic were tediously long and repeated. The physical examination is incomplete. The diagnosis discussion is too simple and lack of order. Conclusions The writing of surgical medical records has its characteristics, which should be evaluated in detail during the teaching and training of medical students. Combined with these characteristics, the surgical medical records should be taught and written under the rules of standard, ordered and integrated.