Abstract: Objective Construct and identify SHH-N gene adeno-associated virus vector and detect its effections on genes related to proliferation in neural stem cells(NSCs).Methods Isolated and cultured the neural stem cells in the subventricular zone of postnatal rat brain, SHH-N was gained by clone.pSNAV2.0-CMV-SHH-N-IRES-EGFP was established by using enzyme cutting and ligation , and then transfecting to packaging cell line 293T to acquire virus. Real time PCR was performed after SHH-N was infected neural stem cells by rAAV-SHH-N-EGFP vector for 48 hours, SHH-N gene expression of infected cells after 2 week was obversed by fluorescence microscope. Result (1)SHH-N gene was coincident with NCBI report.(2) pSNAV2.0-CMV-SHH-N-IRES-EGFP expression vector and rAAV-SHH-N-EGFP vector was successful established and packaged.(3) Real time PCR was performed after SHH-N infection for 48 hour, induction of Nmyc and Gli1 in rAAV-SHH-N-EGFP -treated group was enhanced compared to control group.Conclusion rAAV-SHH -N-EGFP vector had been successfully established and it can stably express in neural stem cells. Forced expression of SHH-N in NSCs resulted in enhanced induction of Nmyc and Gli1.