Abstract: Objective Role of tumor necrosis factor-α(TNF-α) during hyperthermia- induced tumor invasiveness decreases process. Methods Immunohistochemical method detect proliferating cell nuclear antigen(PCNA) of glioma tissue treated by hyperthemia/TNF-α/normal saline(NS), radioimmunoassay monitoring TNF-α content of culture medium and brain glioma tissue after heat treated tumor-bearing rat and rat glioma cell line (C6 cell). Established a tumor model using Transwell, crystal violet staining determine tumor invasiveness. Electron microscope of tumor vascular endothelial cell apoptosis. Results Hyperthemia not only increased the content of TNF-α of C6 rat glioma cell culture medium and tumor tissue, but also decreased glioma invasiveness. Both reached the peak at 120 minute after hyperthermia(P＜0.01). Hyperthemia and TNF-α used respectively on the tumor-bearing rats, all can caused apoptosis of tumor vasculature endothelial cells. TNF-α caused tumor vascular endothelial cell apoptosis levels had the same trend with TNF-α content of C6 cell culture fluid after hyperthemia. Conclusion Hyperthemia induced tumor vascular endothelial cell apoptosis and inhibit tumor invasiveness may be to increased TNF-α content in tumor tissue.