LncRNA(HOTAIR)/miR-206/TAGLN2信号轴调控甲状腺乳头状癌细胞的侵袭

doi:10.16352/j.issn.1001-6325.2023.11.1642

基础医学与临床 ›› 2023, Vol. 43 ›› Issue (11): 1642-1647.doi: 10.16352/j.issn.1001-6325.2023.11.1642

LncRNA(HOTAIR)/miR-206/TAGLN2信号轴调控甲状腺乳头状癌细胞的侵袭

侯丽杰^1,2, 马承旭^1,2, 王晶宇³, 汤旭磊^1,2, 傅松波^1,2*

兰州大学 1.第一临床医学院; 3.基础医学院病理生理教研室,甘肃兰州 730000;
2.兰州大学第一医院内分泌科,甘肃兰州 730000

收稿日期:2022-10-04 修回日期:2023-06-20 出版日期:2023-11-05 发布日期:2023-10-30
通讯作者: ^*fusb@lzu.edu.cn
基金资助:
甘肃省自然科学基金(20JR10RA681,17JR5RA214);甘肃省内分泌疾病临床医学研究中心建设(20JR10FA667)

LncRNA (HOTAIR) /miR-206/TAGLN2 signal axis regulates the invasion of papillarythyroid carcinoma cells

HOU Lijie^1,2, MA Chengxu^1,2, WANG Jingyu³, TANG Xulei^1,2, FU Songbo^1,2*

1.The First Clinical School of Medicine; 3. Department of Pathophysiology, School of Basic Medicine, Lanzhou University, Lanzhou 730000;
2. Department of Endocrinology, the First Hospital of Lanzhou University, Lanzhou 730000, China

Received:2022-10-04 Revised:2023-06-20 Online:2023-11-05 Published:2023-10-30
Contact: ^*fusb@lzu.edu.cn

摘要/Abstract

摘要： 目的探讨lncRNA(HOTAIR)/miR-206/TAGLN2信号轴调控人甲状腺乳头状癌(PTC)细胞系TPC-1侵袭的分子机制。方法收集兰州大学第一医院甲状腺乳头状癌组织和癌旁组织,用RT-qPCR和Western blot检测PTC组织和癌旁组织中HOTAIR、miR-206和TAGLN2的表达;构建miR-206过表达和HOTAIR低表达的TPC-1细胞。RT-qPCR和Western blot检测mRNA和蛋白的表达,Transwell小室法实验检测TPC-1细胞系的侵袭能力。结果与癌旁组织相比PTC组织中HOTAIR表达量增加2.48倍,miR-206-5p表达量降低至0.6,TAGLN2及其蛋白表达量增加(P＜0.05);过表达miR-206后,TPC-1细胞中TAGLN2的蛋白表达量减少(P＜0.05),且TPC-1细胞的侵袭能力受到了抑制。敲低HOTAIR后,TPC-1细胞中miR-206 表达增加,TAGLN2表达降低,同时TPC-1细胞的侵袭能力也明显降低(P＜0.05)。结论在甲状腺乳头状癌中,降低HOTAIR的表达水平,可促成miR-206的过表达,进一步抑制TAGLN2的表达,最终可降低甲状腺癌的侵袭能力。

关键词: 甲状腺乳头状癌, 侵袭, HOTAIR, miR-206, TAGLN2

Abstract: Objective To investigate the molecular mechanism of lncRNA (HOTAIR)/miR-206/TAGLN2 signaling axis in regulating the invasion of papillary thyroid carcinoma cells line TPC-1. Methods The papillary thyroid carcinoma(PTC) and paracancerous tissues were collected from the First Hospital of Lanzhou University, and the expression of lncRNA (HOTAIR), miR-206 and TAGLN2 in PTC tissues as well as paracancerous tissues was detected by RT-qPCR and Western blot; The thyroid papillary cancer cells with miR-206 over-expression and HOTAIR under-expression were constructed to detect the expression of target gene and its effect on the invasion of TPC-1 cells by Transwell assay. Results Compared with the adjacent tissue, the expression of HOTAIR in papilla-rythyroid cancer tissue was increased by 2.48 times. miR-206-5p was decreased down to 0.6,and the expression of TAGLN2 was increased (P＜0.05). After over-expression of miR-206, the protein expression of TAGLN2 was decreased in TPC-1 cell, and the invasion of TPC-1 cells was inhibited (P＜0.05). Knockdown of HOTAIR increased expression of miR-206 in TPC-1 cells and decreased the expression of TAGLN2. The Transwell assay showed that the invasion of TPC-1 cells was significantly reduced (P＜0.05). Conclusions In PTC, reducing the expression of HOTAIR results in over-expression of miR-206 then further inhibits the expression of TAGLN2. The biological outcome is inhibition of invasion of thyroid cancer.

Key words: papillary thyroid carcinoma, invasion, HOTAIR, miR-206, TAGLN2

中图分类号:

R736.1

侯丽杰, 马承旭, 王晶宇, 汤旭磊, 傅松波. LncRNA(HOTAIR)/miR-206/TAGLN2信号轴调控甲状腺乳头状癌细胞的侵袭[J]. 基础医学与临床, 2023, 43(11): 1642-1647.

HOU Lijie, MA Chengxu, WANG Jingyu, TANG Xulei, FU Songbo. LncRNA (HOTAIR) /miR-206/TAGLN2 signal axis regulates the invasion of papillarythyroid carcinoma cells[J]. Basic & Clinical Medicine, 2023, 43(11): 1642-1647.

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参考文献

[1]Sung H, Ferlay J, Siegel RL, et al. Global Cancer Statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2021, 71: 209-249.
[2]Ullmann TM, Gray KD, Moore MD, et al. Current controversies and future directions in the diagnosis and management of differentiated thyroid cancers[J]. Gland Surg, 2018, 7: 473-486.
[3]Zeng C, Zhang H. Mechanisms of Transgelin-2 in Tumorigenesis[J]. Discov Med, 2021,32:23-28.
[4]Yang R, Wang D, Han S, et al. miR-206 suppresses the deterioration of intrahepatic cholangiocarcinoma and promotes sensitivity to chemotherapy by inhibiting interactions with stromal CAFs[J]. Int J Biol Sci, 2022,18:4466-4467.
[5]Liao M, Peng L. miR-206 may suppress non-small lung cancer metastasis by targeting CORO1C[J]. Cell Mol Biol Lett,2020,25:22:1-13.
[6]Li T, Qin Y, Zhen Z, et al. Long non-coding RNA HOTAIR/microRNA-206 sponge regulates STC2 and further influences cell biological functions in head and neck squam-ous cell carcinoma[J]. Cell Prolif, 2019, 52:1-13.
[7]Kuo FC, Wang YT, Liu CH, et al. LncRNA HOTAIR impairs the prognosis of papillary thyroid cancer via regulating cellular malignancy and epigenetically suppressing DLX1[J]. Cancer Cell Int,2022,22:1-13.
[8]Dvorakova M, Nenutil R, Bouchal P. Transgelins, cytoskeletal proteins implicated in different aspects of cancer development[J]. Expert Rev Proteomics, 2014, 11: 149-165.
[9]Huang Y, Zhang H, Wang L, et al. miR-613 inhibits the proliferation, migration, and invasion of papillary thyroid carcinoma cells by directly targeting TAGLN2[J]. Cancer Cell Int, 2021, 21: 1-13.
[10]Fu S, Ma C, Tang X, et al. miR-192-5p inhibits proliferation, migration, and invasion in papillary thyroid carcinoma cells by regulation of SH3RF3[J]. Biosci Rep, 2021, 41: BSR20210342:1-12.
[11]郑婷华,王茜,杨小杰,等. 长链非编码RNA HOTAIR在宫颈癌患者组织及HeLa细胞中的表达和影响[J]. 基础医学与临床, 2016. 36: 94-98.
[12]Mahmoudian-Sani MR, Jalali A, Jamshidi M, et al. Long non-coding RNAs in thyroid cancer: implications for pathogenesis, diagnosis, and therapy[J]. Oncol Res Treat, 2019, 42: 136-142.

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LncRNA(HOTAIR)/miR-206/TAGLN2信号轴调控甲状腺乳头状癌细胞的侵袭

LncRNA (HOTAIR) /miR-206/TAGLN2 signal axis regulates the invasion of papillarythyroid carcinoma cells

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