敲除EZH2抑制人宫颈癌细胞系迁移和侵袭

doi:10.16352/j.issn.1001-6325.2022.03.004

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (3): 395-400.doi: 10.16352/j.issn.1001-6325.2022.03.004

敲除EZH2抑制人宫颈癌细胞系迁移和侵袭

陈茜¹, 刘宪¹, 崔南¹, 翟阳^2*

1.西安交通大学第一附属医院生殖医学科,陕西西安 710061;
2.陕西省肿瘤医院肿瘤内科, 陕西西安 710061

收稿日期:2021-03-30 修回日期:2021-08-06 出版日期:2022-03-05 发布日期:2022-03-04
通讯作者: ^* zhaiyang2020@126.com
基金资助:
国家自然科学基金(81672910)

Knockout of EZH2 inhibits migration and invasion of human cervical cancer cell lines

CHEN Qian¹, LIU Xian¹, CUI Nan¹, ZHAI Yang^2*

1. Department of Reproductive Medicine, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061;
2. Department of Oncology, Tumor Hospital of Shaanxi Province, Xi'an 710061, China

Received:2021-03-30 Revised:2021-08-06 Online:2022-03-05 Published:2022-03-04
Contact: ^* zhaiyang2020@126.com

摘要/Abstract

摘要： 目的研究zeste基因同源蛋白2(EZH2)对人宫颈癌细胞系迁移和侵袭的影响,并初步探讨其作用机制。方法利用CRISPR/Cas9敲除人宫颈癌HeLa和人子宫颈鳞癌SiHa细胞系中EZH2的表达。划痕实验检测细胞迁移;Transwell小室法检测细胞体外迁移和侵袭;实时荧光定量PCR 检测STAT3 mRNA 水平;Western blot 检测STAT3、p-STAT3蛋白表达。结果 CRISPR/Cas9能够敲除EZH2表达;敲除EZH2组中,HeLa和SiHa细胞的划痕愈合率24 h与48 h和Transwell小室迁移和侵袭穿膜细胞数目均低于对照组(P＜0.05)。与对照组相比,敲除EZH2组的p-STAT3 蛋白水平明显降低(P＜0.05)。结论 EZH2在宫颈癌中发挥促进宫颈癌细胞迁移和侵袭的作用,可能与靶向调控STAT3通路有关,具有作为宫颈癌治疗新靶点的潜能。

关键词: 宫颈癌, EZH2, 迁移和侵袭, STAT3信号通路

Abstract: Objective To explore the effect of EZH2 on the migration and invasion of human cervical cancer cell lines and its mechanism. Methods CRISPR/Cas9 was used to knockout EZH2 expression in HeLa and SiHa cell lines. The migration was detected by scratch test, and the migration and invasion in vitro was detected by Transwell migration and invasion test. The STAT3 mRNA level was detected by real-time fluorescence quantitative PCR, and the expression of STAT3 and p-STAT3 proteins was detected by Western blot. Results CRISPR/Cas9 could knockout EZH2 expression. The wound healing rate of HeLa and SiHa cells in EZH2 knockout group at 24 h and 48 h, and the counting of transmembrane cell migration and invasion in Transwell chamber were lower than those in control group(P＜0.05). Compared with the control group, the protein level of p-STAT3 in EZH2 knockout group was significantly decreased (P＜0.05). Conclusions EZH2 plays an important role in promoting the migration and invasion of cervical cancer cells, which may be related to the targeted regulation of STAT3 pathway, and is a potential target in the treatment of cervical cancer.

Key words: cervical cancer, EZH2, migration and invasion, STAT3 signaling pathway

中图分类号:

R737.33

陈茜, 刘宪, 崔南, 翟阳. 敲除EZH2抑制人宫颈癌细胞系迁移和侵袭[J]. 基础医学与临床, 2022, 42(3): 395-400.

CHEN Qian, LIU Xian, CUI Nan, ZHAI Yang. Knockout of EZH2 inhibits migration and invasion of human cervical cancer cell lines[J]. Basic & Clinical Medicine, 2022, 42(3): 395-400.

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敲除EZH2抑制人宫颈癌细胞系迁移和侵袭

Knockout of EZH2 inhibits migration and invasion of human cervical cancer cell lines

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