上调RAI2表达抑制人子宫内膜癌细胞系HEC-1-A和KLE迁移与侵袭

doi:10.16352/j.issn.1001-6325.2023.10.1537

基础医学与临床 ›› 2023, Vol. 43 ›› Issue (10): 1537-1541.doi: 10.16352/j.issn.1001-6325.2023.10.1537

上调RAI2表达抑制人子宫内膜癌细胞系HEC-1-A和KLE迁移与侵袭

聂丹^1*, 汪春燕¹, 李征宇²

1.西南医科大学附属医院妇科,四川泸州 646000;
2.四川大学华西第二医院妇科,四川成都 610041

收稿日期:2023-03-01 修回日期:2023-07-18 出版日期:2023-10-05 发布日期:2023-10-05
通讯作者: *niedan@swmu.edu.cn
基金资助:
泸州市科技计划项目(2019-JYJ-56)

Upregulated RAI2 inhibits migration and invasion of endometrial cancer cell lines HEC-1-A and KLE

NIE Dan^1*, WANG Chunyan¹, LI Zhengyu²

1. Department of Gynecology, the Affiliated Hospital of Southwest Medical University, Luzhou 646000;
2. Department of Gynecology, the West China Second University Hospital of Sichuan University, Chengdu 610041, China

Received:2023-03-01 Revised:2023-07-18 Online:2023-10-05 Published:2023-10-05
Contact: *niedan@swmu.edu.cn

摘要/Abstract

摘要： 目的检测上调维甲酸诱导基因2(retinoic acid-induced 2,RAI2)表达对子宫内膜癌细胞系迁移、侵袭能力的影响。方法选取子宫内膜癌细胞系HEC-1-A和KLE为研究对象,划痕实验、Transwell小室实验检测细胞迁移、侵袭能力。免疫荧光实验、Western blot检测RAI2、E-cadherin、vimentin的蛋白表达。结果子宫内膜癌细胞系HEC-1-A和KLE中RAI2表达水平上调后,LV-RAI2组子宫内膜癌细胞迁移与侵袭能力减弱(P＜0.05),RAI2、E-cadherin表达水平升高,vimentin表达水平降低(P＜0.05)。结论上调RAI2表达可能通过上皮间质转化调控抑制子宫内膜癌细胞迁移与侵袭能力。

关键词: 子宫内膜癌, 维甲酸诱导基因2, 迁移, 侵袭

Abstract: Objective To investigate the effect of upregulation of retinoic acid-induced 2 (RAI2) expression on the migration and invasion of endometrial cancer cells. Methods The endometrial cancer cell lines HEC-1-A and KLE were used for this study. Scratch assay and Transwell chamber assay were used to evaluate the migration and invasion of endometrial cancer cells. Immunofluorescence and Western blot were used to detect the expression of RAI2, E-cadherin, and vimentin. Results The upregulation of RAI2 expression in HEC-1-A and KLE cells significantly reduced migration and invasion ability (P＜0.05). The expression levels of RAI2 and E-cadherin were increased, while the vimentin expression level was decreased (P＜0.05). Conclusions Upregulation of RAI2 expression may inhibit endometrial cancer cell migration and invasion through epithelial-mesenchymal transition.

Key words: endometrial cancer, retinoic acid-induced 2(RAI2), migration, invasion

中图分类号:

R711.7

聂丹, 汪春燕, 李征宇. 上调RAI2表达抑制人子宫内膜癌细胞系HEC-1-A和KLE迁移与侵袭[J]. 基础医学与临床, 2023, 43(10): 1537-1541.

NIE Dan, WANG Chunyan, LI Zhengyu. Upregulated RAI2 inhibits migration and invasion of endometrial cancer cell lines HEC-1-A and KLE[J]. Basic & Clinical Medicine, 2023, 43(10): 1537-1541.

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上调RAI2表达抑制人子宫内膜癌细胞系HEC-1-A和KLE迁移与侵袭

Upregulated RAI2 inhibits migration and invasion of endometrial cancer cell lines HEC-1-A and KLE

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