基础医学与临床 ›› 2022, Vol. 42 ›› Issue (10): 1522-1527.doi: 10.16352/j.issn.1001-6325.2022.10.1522

• 研究论文 • 上一篇    下一篇

EPAS1 siRNA抑制人肾透明细胞癌细胞系HiMet-ccRCC的增殖、迁移及侵袭

陈海滨, 赵建军*, 谭超   

  1. 河北工程大学附属医院 泌尿外二科, 河北 邯郸 056004
  • 收稿日期:2021-07-01 修回日期:2021-11-12 出版日期:2022-10-05 发布日期:2022-09-23
  • 通讯作者: * 1182642227@qq.com
  • 基金资助:
    河北省卫生健康委员会基金(20200591)

EPAS1 siRNA inhibits the proliferation, migration and invasion of human renal clear cell carcinoma cell line HiMet-ccRCC

CHEN Hai-bin, ZHAO Jian-jun*, TAN Chao   

  1. Department of Urology, the Affiliated Hospital of Hebei University of Engineering, Handan 056004, China
  • Received:2021-07-01 Revised:2021-11-12 Online:2022-10-05 Published:2022-09-23
  • Contact: * 1182642227@qq.com

摘要: 目的 探讨沉默内皮PAS区域蛋白1(EPAS1)对抑制人肾透明细胞癌细胞系HiMet-ccRCC增殖、迁移和侵袭的影响及可能机制。方法 将HiMet-ccRCC细胞分为对照(Con)组、阴性对照(NC)组、沉默EPAS1表达组(EPAS1 siRNA组)。用RT-qPCR检测HiMet-ccRCC细胞EPAS1 mRNA水平;MTT法检测HiMet-ccRCC细胞增殖;Transwell小室法检测HiMet-ccRCC细胞迁移、侵袭能力;蛋白质印迹法检测E-cadherin、N-cadherin、MMP-9、PCNA表达。结果 与对照组、阴性对照组比较,EPAS1 siRNA组HiMet-ccRCC细胞中EPAS1表达水平显著降低(P<0.05),HiMet-ccRCC细胞增殖抑制率明显升高、侵袭及迁移细胞数明显减少(P<0.05);与对照组、阴性对照组比较,EPAS1 siRNA组HiMet-ccRCC细胞E-cadherin蛋白表达上调(P<0.05),N-cadherin、MMP-9、PCNA蛋白表达下调(P<0.05)。结论 沉默EPAS1可抑制HiMet-ccRCC细胞增殖、迁移及侵袭,EPAS1可能是肾透明细胞癌基因治疗的潜在生物靶标。

关键词: 内皮PAS区域蛋白1, 肾透明细胞癌, 增殖, 迁移, 侵袭

Abstract: Objective To explore the effects of silencing endothelial PAS domain-containing protein 1 (EPAS1) on the proliferation, migration and invasion of human renal clear cell carcinoma cell line HiMet-ccRCC and to discuss the possible mechanism. Methods HiMet-ccRCC cells were divided into control (Con) group, negative control (NC) group and silencing EPAS1 expression group (EPAS1 siRNA group). RT-qPCR was used to detect the EPAS1 mRNA level in HiMet-ccRCC cells; MTT method was used to detect the proliferation of HiMet-ccRCC cells; Transwell experiment was used to detect the migration and invasion ability of HiMet-ccRCC cells; Western blot was used to detect the expression of E-cadherin,N-cadherin,MMP-9,PCNA. Results Compared with Con and NC group, the expression level of EPAS1 in HiMet-ccRCC cells in the EPAS1 siRNA group was significantly reduced (P<0.05), the proliferation inhibition rate of HiMet-ccRCC cells was significantly increased and the counting of invasion and migration cells was significantly reduced (P<0.05), the protein expression of E-cadherin in HiMet-ccRCC cells from EPAS1 siRNA group increased (P<0.05)and the protein expression of N-cadherin, MMP-9 and PCNA reduced(P<0.05). Conclusions Silencing EPAS1 could inhibit the proliferation, migration and invasion of HiMet-ccRCC cells and EPAS1 may be a potential biological target for gene therapy of renal clear cell carcinoma.

Key words: endothelial PAS domain-containing protein 1, renal clear cell carcinoma, proliferation, migration, invasion

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