骨髓间充质干细胞来源的外泌体缓解低氧/复氧引起的心肌细胞损伤

doi:10.16352/j.issn.1001-6325.2025.12.1557

基础医学与临床 ›› 2025, Vol. 45 ›› Issue (12): 1557-1564.doi: 10.16352/j.issn.1001-6325.2025.12.1557

骨髓间充质干细胞来源的外泌体缓解低氧/复氧引起的心肌细胞损伤

文雯¹, 刘晨曦¹, 陈双景², 陆晓炯¹, 靳志涛³, 张铮^3*

1.锦州医科大学火箭军特色医学中心研究生培养基地心血管内科,北京 100088;
中国人民解放军火箭军特色医学中心 2.研究部,3.心血管内科,北京 100088

收稿日期:2024-11-13 修回日期:2025-03-25 出版日期:2025-12-05 发布日期:2025-11-25
通讯作者: ^*faithword@163.com
基金资助:
北京市自然科学基金(7222229)

Exosomes derived from bone marrow mesenchymal stem cells alleviate hypoxia/reoxygenation-induced cardiomyocyte injury

WEN Wen¹, LIU Chenxi¹, CHEN Shuangjing², LU Xiaojiong¹, JIN Zhitao³, ZHANG Zheng^3*

1. Department of Cardiology, Graduate Training Base of Jinzhou Medical University, PLA Rocket Force Characteristic Medical Center, Beijing 100088;
2. Research Department; 3. Department of Cardiology, PLA Rocket Force Characteristic Medical Center, Beijing 100088, China

Received:2024-11-13 Revised:2025-03-25 Online:2025-12-05 Published:2025-11-25
Contact: ^*faithword@163.com

摘要/Abstract

摘要： 目的探讨骨髓间充质干细胞(BMMSCs)来源的外泌体(BMMSCs-Exo)对低氧/复氧(H/R)诱导大鼠心肌细胞系(H9c2)损伤的作用及机制。方法高速离心法分离BMMSCs-Exo。将细胞分为对照组(control)、H/R组和H/R+BMMSCs-Exo组(H/R+Exo),低氧12 h复氧6 h构建细胞低氧/复氧(H/R)损伤模型。流式细胞术检测细胞凋亡,DHE染色评估细胞ROS水平,JC-1免疫荧光染色检测线粒体膜电位,Western blot检测线粒体自噬相关蛋白。结果 BMMSCs-Exo处理能显著减轻氧化应激,恢复线粒体膜电位,降低线粒体自噬水平,并有效减少心肌细胞凋亡。结论骨髓间充质干细胞来源的外泌体缓解H/R诱导的心肌细胞损伤。

关键词: 外泌体, 低氧/复氧, 氧化应激, 线粒体, 凋亡

Abstract: Objective To explore the effects and mechanisms of bone marrow mesenchymal stem cell(BMMSC)-derived exosomes (BMMSC-Exo) on hypoxia/reoxygenation (H/R)-induced injury in rat cardiomyocyte cell line (H9c2). Methods BMMSC-Exosomes were isolated by ultracentrifugation. The cells were divided into three groups: control,H/R,and H/R+BMMSC-Exo (H/R+Exo). A hypoxia/reoxygenation (H/R) injury model was established by exposing cells to 12 hours of hypoxia followed by 6 hours of reoxygenation. Flow cytometry was used to detect cell apoptosis,DHE staining was used to assess cellular ROS levels,JC-1 immunofluorescence staining was used to evaluate mitochondrial membrane potential,and Western blot was used to detect mitochondrial autophagy-related proteins. Results BMMSC-Exo treatment significantly alleviated oxidative stress,restored mitochondrial membrane potential,reduced mitochondrial autophagy levels,and effectively decreased cardiomyocyte apoptosis. Conclusions Bone marrow mesenchymal stem cell-derived exosomes alleviate H/R-induced cardiomyocyte injury.

Key words: exosomes, hypoxia/reoxygenation, oxidative stress, mitochondria, apoptosis

中图分类号:

R541.4

文雯, 刘晨曦, 陈双景, 陆晓炯, 靳志涛, 张铮. 骨髓间充质干细胞来源的外泌体缓解低氧/复氧引起的心肌细胞损伤[J]. 基础医学与临床, 2025, 45(12): 1557-1564.

WEN Wen, LIU Chenxi, CHEN Shuangjing, LU Xiaojiong, JIN Zhitao, ZHANG Zheng. Exosomes derived from bone marrow mesenchymal stem cells alleviate hypoxia/reoxygenation-induced cardiomyocyte injury[J]. Basic & Clinical Medicine, 2025, 45(12): 1557-1564.

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骨髓间充质干细胞来源的外泌体缓解低氧/复氧引起的心肌细胞损伤

Exosomes derived from bone marrow mesenchymal stem cells alleviate hypoxia/reoxygenation-induced cardiomyocyte injury

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