甘露醇促进人肺鳞癌细胞系NCI-H292凋亡

doi:10.16352/j.issn.1001-6325.2025.03.0336

基础医学与临床 ›› 2025, Vol. 45 ›› Issue (3): 336-340.doi: 10.16352/j.issn.1001-6325.2025.03.0336

甘露醇促进人肺鳞癌细胞系NCI-H292凋亡

李黄羿, 黄道航, 张文玉, 黄鼎铭, 黄其春^*

广西医科大学附属肿瘤医院药剂科,广西南宁 530021

收稿日期:2024-08-16 修回日期:2024-10-31 发布日期:2025-02-25
通讯作者: ^*102140912@qq.com
基金资助:
2022年度广西高校中青年教师科研基础能力提升项目(2022KY0106);2022年度广西壮族自治区卫生健康委西医类自筹经费科研课题(Z-A20220676)

Mannitol promotes the apoptosis ofhuman lung squamous carcinoma cell line NCI-H292

LI Huangyi, HUANG Daohang, ZHANG Wenyu, HUANG Dingming, HUANG Qichun^*

Department of Pharmacy, Guangxi Medical University Affiliated Cancer Hospital, Nanning 530021, China

Received:2024-08-16 Revised:2024-10-31 Published:2025-02-25

摘要/Abstract

摘要： 目的探讨20%甘露醇注射液对人肺鳞癌细胞系NCI-H292增殖和凋亡的作用及其可能机制。方法使用20%及以下不同浓度甘露醇处理NCI-H292细胞,CCK-8法检测NCI-H292细胞增殖和活性;流式细胞测量术检测细胞凋亡;Western blot检测线粒体凋亡途径相关蛋白Bcl-2和Bax的表达。结果与对照组比较,甘露醇呈剂量和时间依赖性抑制细胞增殖(P＜0.05); 20%甘露醇可诱导细胞凋亡(P＜0.05);经20%甘露醇处理后的细胞中Bcl-2的表达水平均显著降低,Bax的表达水平均明显升高(P＜0.05)。结论临床上常用的20%甘露醇注射液可以诱导肺鳞癌细胞系NCI-H292凋亡,其机制与线粒体途径相关蛋白Bcl-2、Bax有关。

关键词: 甘露醇, 肺鳞癌, 凋亡, Bcl-2, Bax

Abstract: Objective To investigate the effects of 20% mannitol injection on the proliferation and apoptosis of human lung squamous carcinoma cell line NCI-H292 and its possible mechanisms. Methods NCI-H292 cells were incubated with 20% and lower concentrations of mannitol. Cell proliferation and viability were detected by CCK-8 assay. Cell apoptosis was detected by flow cytometry and the expression of mitochondrial apoptosis pathway-related proteins Bcl-2 and Bax was detected by Western blot. Results Compared with the control group, mannitol inhibited cell proliferation in a dose- and time-dependent manner (P＜ 0.05), 20% mannitol could induce cell apoptosis (P＜ 0.05). The expression level of Bcl-2 was significantly reduced, while that expression level of Bax was significantly increased in cells incubated with 20% mannitol (P＜ 0.05). Conclusions Clinically used 20% mannitol injection can induce apoptosis of lung squamous carcinoma cell line NCI-H292. Its mechanism is related to mitochondrial pathway-related proteins Bcl-2 and Bax.

Key words: mannitol, lung squamous cell carcinoma, apoptosis, Bcl-2, Bax

中图分类号:

R73-36
R982

李黄羿, 黄道航, 张文玉, 黄鼎铭, 黄其春. 甘露醇促进人肺鳞癌细胞系NCI-H292凋亡[J]. 基础医学与临床, 2025, 45(3): 336-340.

LI Huangyi, HUANG Daohang, ZHANG Wenyu, HUANG Dingming, HUANG Qichun. Mannitol promotes the apoptosis ofhuman lung squamous carcinoma cell line NCI-H292[J]. Basic & Clinical Medicine, 2025, 45(3): 336-340.

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甘露醇促进人肺鳞癌细胞系NCI-H292凋亡

Mannitol promotes the apoptosis ofhuman lung squamous carcinoma cell line NCI-H292

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