LncRNA HIF1A-AS1通过干预HIF1A促进胃癌细胞系增殖

doi:10.16352/j.issn.1001-6325.2025.05.0627

基础医学与临床 ›› 2025, Vol. 45 ›› Issue (5): 627-636.doi: 10.16352/j.issn.1001-6325.2025.05.0627

LncRNA HIF1A-AS1通过干预HIF1A促进胃癌细胞系增殖

殷利军¹, 卜阳¹, 昂格勒玛², 宋琳琳^3*

1.宁夏回族自治区人民医院普通外科中心, 宁夏银川 750000;
2.宁夏医科大学第一临床学院, 宁夏银川 750000;
3.宁夏医科大学总医院心脑血管病医院妇科,宁夏银川 750000

收稿日期:2024-07-29 修回日期:2024-11-08 出版日期:2025-05-05 发布日期:2025-04-23
通讯作者: ^* 42976260@qq.com
基金资助:
宁夏自然科学基金(2022AAC03376)

LncRNA HIF1A-AS1 promotes the proliferation of gastric cancer cell lines through interfering with HIF1A

YIN Lijun¹, BO Yang¹, Anggelema², SONG Linlin^3*

1. Center of General Surgery, People′s Hospital of Ningxia Hui Autonomous Region, Yinchuan 750000;
2. The First Clinical College of Ningxia Medical University, Yinchuan 750000;
3. Department of Gynecology,Cardio-Cerebrovascular Disease Hospital, General Hospital of Ningxia Medical University, Yinchuan 750000, China

Received:2024-07-29 Revised:2024-11-08 Online:2025-05-05 Published:2025-04-23
Contact: ^* 42976260@qq.com

摘要/Abstract

摘要： 目的探索HIF1A-AS1对胃癌细胞系作用及其可能的作用机制。方法生物信息学分析胃癌组织中HIF1A-AS1的表达情况;构建HIF1A-AS1的过表达和沉默BGC-823、MKN28细胞株,real-time PCR检测HIF1A-AS1的表达情况,CCK-8法完成细胞增殖检测,流式细胞测量术完成细胞凋亡检测,Western blot完成HIF1A蛋白在细胞质和细胞核中的表达检测,细胞免疫荧光完成HIF1A蛋白的入核检测。结果成功构建了HIF1A-AS1过表达和沉默的BGC-823和MKN28细胞模型。CCK-8法结果显示,HIF1A-AS1的沉默使细胞增殖下降(P＜0.05),HIF1A-AS1过表达则细胞增殖显著上升(P＜0.05);细胞凋亡结果显示HIF1A-AS1的沉默使细胞凋亡显著上升(P＜0.05),HIF1A-AS1-over组细胞凋亡显著降低(P＜0.05);细胞免疫荧光显示结果显示HIF1A-AS1的沉默抑制HIF1A蛋白的入核现象,过表达则促进入核现象的发生;Western blot结果显示,HIF1A-AS1沉默使HIF1A细胞质蛋白的表达显著降低(P＜0.05),HIF1A-AS1过表达则促使HIF1A细胞质蛋白的表达显著上升(P＜0.05);HIF1A-AS1沉默促使HIF1A细胞核蛋白的表达显著降低(P＜0.05),HIF1A-AS1过表达促使HIF1A细胞核蛋白的表达显著上升(P＜0.05)。结论 HIF1A-AS1可能通过调控亲本基因HIF1A的表达促进胃癌细胞系BGC-823、MKN28的增殖,抑制凋亡。

关键词: 亲本基因控调, 入核, 增殖, 凋亡

Abstract: Objective To investigate the effects of HIF1A-AS1 on the proliferation and apoptosis of gastric cancer cells lines BGC-823 and MKN28 and its possible mechanism. Methods The expression of HIF1A-AS1 in gastric cancer tissues was analyzed by bioinformatics. HIF1A-AS1 over expression and silenced BGC-823 and MKN28 cell strains were constructed. HIF1A-AS1 expression was detected by real-time PCR, cell proliferation was detected by CCK-8 assay, and apoptosis was detected by flow cytometry. Western blot was used to detect the expression of HIF1A protein in cytoplasm and nucleus and cellular immunofluorescence was used to detect HIF1A protein into nucleus. Results Models of BGC-823 and MKN28 gastric cancer cells with HIF1A-AS1 over-expression and silence were successfully constructed.HIF1A-AS1 over-expression decreased cell proliferation (P＜0.05), while HIF1A-AS1 over-expression significantly increased cell proliferation (P＜0.05). HIF1A-AS1 silencing significantly increased the apoptosis (P＜0.05), and HIF1A-AS1-over expression significantly decreased the apoptosis (P＜0.05). HIF1A-AS1 silencing inhibited the nucleation of HIF1A protein and over-expression promoted the nucleation of HIF1A protein. HIF1A-AS1 silencing significantly decreased the expression of HIF1A cytoplasmic protein (P＜0.05) and over-expression of HIF1A-AS1 significantly increased the expression of HIF1A cytoplasm protein (P＜0.05). HIF1A-AS1 silencing significantly decreased the expression of HIF1A nuclear protein (P＜0.05) while HIF1A-AS1 over-expression significantly increased the expression of HIF1A nuclear protein (P＜0.05). Conclusions HIF1A-AS1 may promote the proliferation and inhibit apoptosis of BGC-823 and MKN28 gastric cancer cells by regulating the expression of parental gene HIF1A.

Key words: parental gene control, enter the nucleus, proliferation, apoptosis

中图分类号:

R73-3

殷利军, 卜阳, 昂格勒玛, 宋琳琳. LncRNA HIF1A-AS1通过干预HIF1A促进胃癌细胞系增殖[J]. 基础医学与临床, 2025, 45(5): 627-636.

YIN Lijun, BO Yang, Anggelema, SONG Linlin. LncRNA HIF1A-AS1 promotes the proliferation of gastric cancer cell lines through interfering with HIF1A[J]. Basic & Clinical Medicine, 2025, 45(5): 627-636.

参考文献 15

[1]	Bray F, Laversanne M, Sung H, et al. Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2024,74:229-263. doi: 10.3322/caac.21834.
[2]	Machlowska J, Baj J, Sitarz M, et al. Gastric cancer: epidemiology, risk factors, classification, genomic characteristics and treatment strategies[J]. Int J Mol Sci, 2020,21:4012. doi:10.3390/ijms21114012.
[3]	Yan H, Bu P. Non-coding RNA in cancer[J]. Essays Biochem,2021,65:625-639.
[4]	肖宁,林玮键,龙永福.长链非编码RNA HIF1A-AS1的研究现状与进展[J].现代医学与健康研究电子杂志,2021,5:126-128.
[5]	Hong F, Gao Y, Li Y, et al. Inhibition of HIF1A-AS1 promoted starvation-induced hepatocellular carcinoma cell apoptosis by reducing HIF-1α/mTOR-mediated autophagy[J]. World J Surg Oncol, 2020,18:113.doi: 10.1186/s12957-020-01884-x.
[6]	Xu J, Zhang Y, Chu L, et al. Long non-coding RNA HIF1A-AS1 is upregulated in intracranial aneurysms and participates in the regulation of proliferation of vascular smooth muscle cells by upregulating TGF-β1[J]. Exp Ther Med, 2019,17:1797-1801.doi: 10.3892/etm.2018.7144.
[7]	Gao J, Cao R, Mu H. Long non-coding RNA UCA1 may be a novel diagnostic and predictive biomarker in plasma for early gastric cancer[J]. Int J Clin Exp Pathol, 2015,8:12936-12942.
[8]	Gong Z, Yang J, Dong J, et al. LncRNA HIF1A-AS1 regulates the cellular function of HUVECs by globally regulating mRNA and miRNA expression[J]. Front Biosci (Landmark Ed), 2022,27:330. doi: 10.31083/j.fbl2712330.
[9]	Gong W, Tian M, Qiu H, et al. Elevated serum level of lncRNA-HIF1A-AS1 as a novel diagnostic predictor for worse prognosis in colorectal carcinoma[J]. Cancer Biomark, 2017,20:417-424. doi: 10.3233/CBM-170179.
[10]	Tantai J, Hu D, Yang Y, et al. Combined identification of long non-coding RNA XIST and HIF1A-AS1 in serum as an effective screening for non-small cell lung cancer[J]. Int J Clin Exp Pathol, 2015,8:7887-7895.
[11]	Chen D, Wu L, Liu L, et al. Comparison of HIF1A-AS1 and HIF1A-AS2 in regulating HIF-1α and the osteogenic differentiation of PDLCs under hypoxia[J]. Int J Mol Med, 2017,40:1529-1536. doi: 10.3892/ijmm.2017.3138.
[12]	Tantai J, Hu D, Yang Y, et al. Combined identification of long non-coding RNA XIST and HIF1A-AS1 in serum as an effective screening for non-small cell lung cancer[J]. Int J Clin Exp Pathol, 2015,8:7887-7895.
[19]	张广健,高蕊,付军科,等. 多药耐药基因及缺氧诱导因子-1α在食管癌组织中的表达及其临床意义[J]. 西安交通大学学报(医学版), 2010, 31: 724-727.
[20]	Rashid M, Zadeh LR, Baradaran B, et al. Up-down regulation of HIF-1α in cancer progression[J]. Gene, 2021,798:145796. doi: 10.1016/j.gene.2021.145796.
[14]	Maruggi M, Layng FI, Lemos R Jr, et al. Absence of HIF1A leads to glycogen accumulation and an inflam-matory response that enables pancreatic tumor growth[J]. Cancer Res, 2019,79:5839-5848. doi: 10.1158/0008-5472.CAN-18-2994.
[15]	金烨,颜海豪,代璐,等.缺氧诱导因子-1在胃癌中的研究进展[J].中国临床研究,2021,34:405-408.

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LncRNA HIF1A-AS1通过干预HIF1A促进胃癌细胞系增殖

LncRNA HIF1A-AS1 promotes the proliferation of gastric cancer cell lines through interfering with HIF1A

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