基础医学与临床 ›› 2025, Vol. 45 ›› Issue (4): 486-492.doi: 10.16352/j.issn.1001-6325.2025.04.0486

• 研究论文 • 上一篇    下一篇

上调miR-152抑制ox-LDL诱导的人血管平滑肌细胞增殖和迁移

周晶, 屈苗*   

  1. 宝鸡市中心医院 功能科, 陕西 宝鸡 721008
  • 收稿日期:2024-07-31 修回日期:2024-12-25 出版日期:2025-04-05 发布日期:2025-03-24
  • 通讯作者: *qm305266253@163.com

Upregulation of miR-152 inhibits proliferation and migration of human vascular smooth muscle cells induced by ox-LDL

ZHOU Jing, QU Miao*   

  1. Function Department, Baoji Central Hospital, Baoji 721008, China
  • Received:2024-07-31 Revised:2024-12-25 Online:2025-04-05 Published:2025-03-24

摘要: 目的 研究miR-152和Rho相关卷曲螺旋蛋白激酶1(ROCK1)在氧化型低密度脂蛋白(ox-LDL)干预的血管平滑肌细胞(VSMCs)中的作用。方法 通过ox-LDL干预人血管平滑肌细胞CRL-1999建立体外动脉粥样硬化模型,转染miR-152 mimic和ROCK1 siRNA至对应细胞,RT-qPCR检测miR-152和ROCK1 mRNA表达,CCK-8法检测细胞增殖活性,Trasnwell实验检测细胞迁移能力,荧光素酶报告基因检测miR-152和ROCK1的靶向关系。结果 以100 μg/mL ox-LDL处理48 h的CRL-1999细胞增殖率最大;与未被ox-LDL未处理的对照组相比较,ox-LDL诱导组的miR-152相对表达显著降低(P<0.05);转染miR-152 mimic后显著降低了CRL-1999细胞增殖活性和迁移能力(P<0.05);荧光素酶报告基因测定显示miR-152靶向调节ROCK1,miR-152 mimic转染的 CRL-1999细胞中ROCK1 mRNA和蛋白质表达降低(P<0.05);转染ROCK1 siRNA显著降低了CRL-1999细胞增殖活性和迁移能力(P<0.05)。结论 miR-152通过下调ROCK1的表达,抑制了ox-LDL诱导的CRL-1999细胞增殖和迁移,这可能是动脉粥样硬化的潜在治疗靶点。

关键词: miR-152, 动脉粥样硬化, Rho相关卷曲螺旋蛋白激酶1, 血管平滑肌, 增殖, 转移

Abstract: Objective To investigate the roles of miR-152 and Rho-associated coiled-coil kinase 1 (ROCK1) in vascular smooth muscle cells (VSMCs) treated with oxidized low-density lipoprotein (ox-LDL). Methods Human vascular smooth muscle cells CRL-1999 were intervened with ox-LDL to establish an in vitro atherosclerosis model. miR-152 mimic and ROCK1 siRNA were transfected into corresponding cells. RT-qPCR was used to detect the expression of miR-152 and ROCK1 mRNA. The CCK-8 method was used to assess cell proliferation activity, and the Transwell assay was used to test cell migration ability. A luciferase reporter gene assay was conducted to determine the targeting relationship between miR-152 and ROCK1. Results CRL-1999 cells treated with 100 μg/mL ox-LDL for 48 hours showed the maximum proliferation rate. Compared with the untreated control group, the relative expression of miR-152 in the ox-LDL-induced group was significantly decreased (P<0.05). Transfection of miR-152 mimic significantly reduced the proliferation and migration of CRL-1999 cells (P<0.05). The luciferase reporter gene assay indicated that miR-152 targets ROCK1, and expression of ROCK1 mRNA and protein in miR-152 mimic group was reduced(P<0.05). Transfection of ROCK1 siRNA also significantly decreased the proliferation and migration of CRL-1999 cells(P<0.05). Conclusions miR-152 inhibits the ox-LDL induced proliferation and migration of CRL-1999 cells by downregulating ROCK1 expression, which may be a potential therapeutic target for atherosclerosis.

Key words: miR-152, atherosclerosis, Rho-associated coiled-coil kinase 1, vascular smooth muscle, proliferation, migration

中图分类号: