多烯磷脂酰胆碱促进高糖诱导人脐静脉内皮细胞损伤的修复

doi:10.16352/j.issn.1001-6325.2026.01.0071

基础医学与临床 ›› 2026, Vol. 46 ›› Issue (1): 71-77.doi: 10.16352/j.issn.1001-6325.2026.01.0071

多烯磷脂酰胆碱促进高糖诱导人脐静脉内皮细胞损伤的修复

陈星^1*, 李雨龙², 张冰¹, 梁劲杰⁴, 陈姿伊^3*

1.中国人民解放军总医院医疗保障中心药剂科,北京 100853;
2.北京中医药大学临床医学院,北京 100029;
3.中国中医科学院中药资源中心,北京 100700;
4.北京中医药大学生命科学学院,北京 102488

收稿日期:2025-04-06 修回日期:2025-05-27 出版日期:2026-01-05 发布日期:2025-12-29
通讯作者: ^*chenxing115115@126.com;czy9899@163.com
基金资助:
北京中医药大学研究生自主科研课题(90011461220416)

Polyene phosphatidylcholine repairs high glucose-induced human umbilical vein endothelial cells damage

CHEN Xing^1*, LI Yulong², ZHANG Bing¹, LIANG Jinjie⁴, CHEN Ziyi^3*

1. Department of Pharmacy, Medical Supplies Center of Chinese PLA General Hospital,Beijing 100853;
2. School of Clinical Medicine, Beijing University of Chinese Medicine, Beijing 100029;
3. National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700;
4. School of Life Sciences, Beijing University of Chinese Medicine, Beijing 102488, China

Received:2025-04-06 Revised:2025-05-27 Online:2026-01-05 Published:2025-12-29
Contact: ^*chenxing115115@126.com;czy9899@163.com

摘要/Abstract

摘要： 目的探究多烯磷脂酰胆碱(PPC)对高糖诱导人脐静脉内皮细胞(HUVECs)的功能损伤是否有修复作用。方法 MTT法检测PPC对高糖损伤的HUVECs增殖活力的影响。细胞划痕实验和Transwell小室法检测PPC对高糖损伤HUVECs细胞迁移能力的影响。血管形成实验检测PPC对高糖损伤HUVECs分化成血管能力的影响。Western blot检测PPC对高糖损伤的HUVECs中Bax、Bcl-2、caspase-3、VEGFA蛋白表达的影响。结果 MTT实验结果显示,PPC可以显著恢复高糖对HUVECs的增殖抑制作用。细胞划痕实验和Transwell小室法结果显示,PPC可以显著促进高糖损伤HUVECs迁移能力的恢复。血管形成实验结果显示,PPC可以显著促进高糖损伤HUVECs分化形成血管能力的恢复。Western blot结果显示,PPC可以抑制高糖损伤HUVECs内促凋亡蛋白Bax、caspase-3的表达,并促进抗凋亡蛋白Bcl-2的表达。此外,PPC显著促进高糖损伤HUVECs中VEGFA的表达。结论 PPC通过促进VEGFA的表达进而促进高糖损伤HUVECs的增殖、迁移和分化成血管能力的恢复,并且通过Bax/Bcl-2/caspase-3信号通路抑制高糖损伤HUVECs发生细胞凋亡。

关键词: 多烯磷脂酰胆碱, 人脐静脉内皮细胞, 血管内皮生长因子, 凋亡, 血管生成

Abstract: Objective Exploring whether polyene phosphatidylcholine (PPC) has a reparative effect on high glucose-induced functional damage of human umbilical vein endothelial cells (HUVECs). Methods MTT assay was used to detect the effect of PPC on the proliferation activity of HUVECs damaged by high concentration of glucose. Cell scratch assay and Transwell migration assay were used to investigate the effect of PPC on the migration ability of high glucose damaged HUVECs. The angiogenesis experiment was conducted to investigate the effect of PPC on the ability of high concentration glucose-induced HUVECs to differentiate into blood vessels. Western blot was used to detect the effect of PPC on the expression of Bax, Bcl-2, caspase-3, and VEGFA proteins in HUVECs damaged by high concentration of glucose. Results PPC significantly restored the inhibitory effect of glucose on HUVECs proliferation and significantly promoted the recovery of high glucose-damaged HUVECs migration ability. PPC also significantly promoted the recovery of the ability of high glucose-damaged HUVECs to differentiate and form blood vessels. Western blot results showed that PPC inhibited the expression of proapoptotic proteins Bax and caspase-3 in HUVECs tissue. Conclusions PPC promotes the proliferation, migration, and vascular differentiation of high glucose-induced HUVECs by increasing VEGFA expression and inhibits apoptosis of HUVECs damaged by high concentration of glucose through Bax/Bcl-2/caspase-3 signaling pathway.

Key words: polyene phosphatidylcholine, human umbilical vein endothelial cell, vascular endothelial growth factor, apoptosis, angiogenesis

中图分类号:

R285

陈星, 李雨龙, 张冰, 梁劲杰, 陈姿伊. 多烯磷脂酰胆碱促进高糖诱导人脐静脉内皮细胞损伤的修复[J]. 基础医学与临床, 2026, 46(1): 71-77.

CHEN Xing, LI Yulong, ZHANG Bing, LIANG Jinjie, CHEN Ziyi. Polyene phosphatidylcholine repairs high glucose-induced human umbilical vein endothelial cells damage[J]. Basic & Clinical Medicine, 2026, 46(1): 71-77.

参考文献

[1]Reusch JE, Manson JE. Management of type 2 diabetes in 2017: getting to goal[J]. JAMA, 2017,317:1015-1016.
[2]Chen Z, Yuan M, Li H, et al. Succinylated chitosan derivative restore HUVEC cells function damaged by TNF-α and high glucose in vitro and enhanced wound healing[J]. Int J Biol Macromol, 2024, 265:130825. doi: 10.1016/j.ijbiomac.2024.130825.
[3]冉倩,徐蔚蔚,孙航,等.伤口愈合协会2023年《糖尿病足溃疡治疗指南(第3版)》解读[J].护理研究,2025,39:1062-1067.
[4]朱成晟,方水林,石彦波.糖脉宁对高糖诱导人脐静脉内皮细胞损伤的保护作用及AMPK-eNOS通路的影响[J].中国中医药科技,2025,32:25-30.
[5]Mak KM, Shekhar AC. Soybean polyenylphosphatidylcholine (PPC) is beneficial in liver and extrahepatic tissue injury: an update in experimental research[J]. Anat Rec (Hoboken), 2024, 307:2162-2186.
[6]Li Y, Chen A, Li Z, et al. Effectiveness of polyene phosphatidylcholine and its combination with other drugs in patients with liver diseases based on real-world research[J]. Expert Rev Clin Pharmacol, 2022,15:1363-1375.
[7]Li C, Yang X, Quan Y, et al. Network Meta-analysis of different liver protective drugs in the treatment of drug-induced liver injury[J]. Medicine (Baltimore), 2023, 15,102:e36538.doi: 10.1097/MD.0000000000036538.
[8]Lou J, Wu J, Feng M, et al. Exercise promotes angiogenesis by enhancing endothelial cell fatty acid utilization via liver-derived extracellular vesicle miR-122-5p[J]. J Sport Health Sci, 2022,11:495-508.
[9]高砚丽,康黎,韩怡婷,等.舒洛地特通过上调VEGF-VEGFR2对高糖环境下心肌微血管内皮细胞发挥促血管新生作用[J].中国老年学杂志,2025,45:1495-1499.
[10]Pan Q, Xu X, He W, et al. Enrichment of miR-17-5p enhances the protective effects of EPC-EXs on vascular and skeletal muscle injury in a diabetic hind limb ischemia model[J]. Biol Res, 2023,56:16.doi: 10.1186/s40659-023-00418-5.
[11]李蓉,姚国敏,张敏,等.胃饥饿素通过抑制内质网应激减少高糖诱导的视网膜血管内皮细胞凋亡[J].山西医科大学学报,2023,54:1632-1637.
[12]Dogan T, Yildirim BA, Terim Kapakin KA, et al. Protective effects of crocin against gentamicin-induced damage in rat testicular tissue: modulating the levels of NF-κB/TLR-4 and Bax/Bcl-2/caspase-3 signaling pathways[J]. Food Chem Toxicol, 2025, 22;200:115407.doi: 10.1016/j.fct.2025.115407.
[13]Alipour M, Sheikhnejad R, Fouani MH, et al. DNAi-peptide nanohybrid smart particles target BCL-2 oncogene and induce apoptosis in breast cancer cells[J]. Biomed Pharmacother, 2023,166:115299.doi: 10.1016/j.biopha.2023.115299.
[14]肖丽娜,周先荣,张青华.Caspase-3蛋白在非小细胞肺癌中的表达及其与肿瘤细胞凋亡的关系[J].检验医学与临床,2019,16:2034-2036+2041.

[1]	孙迎春, 李建军, 杨再波, 闫俊慧, 刘育硕. IgA肾病患者血清CHI3L1和sFlt-1水平与病情严重程度及预后相关[J]. 基础医学与临床, 2026, 46(1): 22-27.
[2]	李春孟, 孙慧艳, 郑祥坚, 谢尚尚, 林德永, 刘子田. 外源性Ang-1与miR-210共感染的内皮祖细胞腔内移植促进糖尿病慢性下肢缺血血管新生[J]. 基础医学与临床, 2026, 46(1): 46-55.
[3]	李娜, 张鹏, 张楠, 林伟, 刘德峰, 郑继会. 灯盏花乙素减轻椎间盘退变模型大鼠髓核细胞凋亡[J]. 基础医学与临床, 2025, 45(9): 1158-1164.
[4]	安信, 贾麟, 马吾苏木·马木特, 黄志鹏, 卡哈尔曼·斯拉木, 热夏提·热合曼, 海日拉·吾拉木, 库尔班江·阿布力克木, 加素尔·巴吐尔. ANGPT1影响小鼠前列腺癌细胞系RM-1增殖、侵袭及血管生成[J]. 基础医学与临床, 2025, 45(9): 1165-1172.
[5]	于华惠, 杜智勇, 焦晓璐, 吕倩雯, 杨云云, 韩丽杰, 秦彦文. 血管生成素样蛋白8通过激活p53信号通路促进平滑肌细胞凋亡[J]. 基础医学与临床, 2025, 45(7): 882-888.
[6]	孙婷, 邹腾, 杨轶嵩, 刘双萍, 任欣, 王丹. β-榄香酮酸对结肠癌细胞系增殖和侵袭的抑制作用[J]. 基础医学与临床, 2025, 45(6): 748-754.
[7]	张芳龄, 张湲钫, 张立. 右美托咪定减轻丙泊酚诱导的新生大鼠神经损伤[J]. 基础医学与临床, 2025, 45(5): 644-650.
[8]	殷利军, 卜阳, 昂格勒玛, 宋琳琳. LncRNA HIF1A-AS1通过干预HIF1A促进胃癌细胞系增殖[J]. 基础医学与临床, 2025, 45(5): 627-636.
[9]	魏建新, 房彦乐, 鲁雨博, 高宇光, 郎兴, 李静涛, 马新生. 青蒿琥酯抑制肾母细胞瘤细胞系SK-NEP-1增殖和促进凋亡与自噬[J]. 基础医学与临床, 2025, 45(4): 493-498.
[10]	李黄羿, 黄道航, 张文玉, 黄鼎铭, 黄其春. 甘露醇促进人肺鳞癌细胞系NCI-H292凋亡[J]. 基础医学与临床, 2025, 45(3): 336-340.
[11]	文雯, 刘晨曦, 陈双景, 陆晓炯, 靳志涛, 张铮. 骨髓间充质干细胞来源的外泌体缓解低氧/复氧引起的心肌细胞损伤[J]. 基础医学与临床, 2025, 45(12): 1557-1564.
[12]	冯海凉, 孔令华, 代伽茵, 杨振丽, 卞晓翠, 刘玉琴. 原代及转化的人血管内皮细胞模型的建立[J]. 基础医学与临床, 2025, 45(12): 1600-1607.
[13]	李海鹏, 刘一帆, 李珊, 陈道辉, 雷京霖, 胡政. miR-423-5p在缺血性卒中患者血浆中的表达升高[J]. 基础医学与临床, 2025, 45(12): 1572-1579.
[14]	张越, 王君影. 泛凋亡在年龄相关性疾病中的研究进展[J]. 基础医学与临床, 2025, 45(11): 1506-1510.
[15]	甲宗虎, 晋群, 韩淑芳, 胡毓洪, 任昌振, 李云萍, 刘文燕. miR-374c-5p降低过氧化氢诱导的人脐静脉内皮细胞凋亡[J]. 基础医学与临床, 2025, 45(11): 1457-1462.

多烯磷脂酰胆碱促进高糖诱导人脐静脉内皮细胞损伤的修复

Polyene phosphatidylcholine repairs high glucose-induced human umbilical vein endothelial cells damage

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价