miR-374c-5p降低过氧化氢诱导的人脐静脉内皮细胞凋亡

doi:10.16352/j.issn.1001-6325.2025.11.1457

基础医学与临床 ›› 2025, Vol. 45 ›› Issue (11): 1457-1462.doi: 10.16352/j.issn.1001-6325.2025.11.1457

miR-374c-5p降低过氧化氢诱导的人脐静脉内皮细胞凋亡

甲宗虎¹, 晋群^2*, 韩淑芳², 胡毓洪², 任昌振², 李云萍², 刘文燕²

1.锦州医科大学中国人民解放军联勤保障部队第九六〇医院研究生培养基地心内科,山东济南 250031;
2.中国人民解放军联勤保障部队第九六〇医院心内科,山东济南 250031

收稿日期:2024-08-13 修回日期:2025-02-26 出版日期:2025-11-05 发布日期:2025-10-24
通讯作者: ^*b94xlm@163.com
基金资助:
潍坊医学院附属医院科技发展项目(2023FYZ013)

miR-374c-5p reduces hydrogen peroxide induced apoptosis of human umbilical vein endothelial cells

JIA Zonghu¹, JIN Qun^2*, HAN Shufang², HU Yuhong², REN Changzhen², LI Yunping², LIU Wenyan¹

1. Department of Cardiology,Graduate Training Base of the 960^th Hospital of the PLA Joint Logistics Support Force of Jinzhou Medical University, Jinan 250031;
2. Department of Cardiology,the 960^th Hospital of the PLA Joint Logistics Support Force,Jinan 250031, China

Received:2024-08-13 Revised:2025-02-26 Online:2025-11-05 Published:2025-10-24
Contact: ^*b94xlm@163.com

摘要/Abstract

摘要： 目的探究miR-374c-5p对过氧化氢(H₂O₂)诱导的人脐静脉内皮细胞(HUVECs)凋亡的保护作用。方法体外培养HUVECs,将培养细胞分为4组:对照组、H₂O₂组、H₂O₂+miR-374c-5p mimics转染组、H₂O₂ +miR-374c-5p inhibitor转染组。CCK-8法检测细胞增殖率评估细胞活性;TUNEL染色检测细胞凋亡;RT-qPCR检测miR-374c-5p和Fas mRNA的表达;Western blot检测细胞Fas蛋白的表达。结果 1)与对照组相比,H₂O₂组HUVECs的增殖率明显降低,凋亡明显增加(P＜0.001);2)H₂O₂+miR-374c-5p mimics转染组较H₂O₂组增殖率明显增加,凋亡明显降低(P＜0.001);3)H₂O₂ +miR-374c-5p inhibitor转染组较H₂O₂组增殖率明显降低,凋亡明显增加(P＜0.001)。结论 miR-374c-5p降低H₂O₂诱导的人脐静脉内皮细胞凋亡。

关键词: miR-374c-5p, 人脐静脉内皮细胞(HUVECs), 过氧化氢, 氧化应激, 凋亡

Abstract: Objective To explore the protective effect of miR-374c-5p on hydrogen peroxide (H₂O₂) -induced apoptosis of human umbilical vein endothelial cells (HUVECs). Methods HUVECs were cultured in vitro and the harvested cells were divided into four groups: control group, H₂O₂ group, H₂O₂ + miR-374c-5p mimics transfection group, and H₂O₂ + miR-374c-5p inhibitor transfection group. Cell activity was assessed by CCK-8 proliferation rate assay, apoptosis was detected by TUNEL staining microscopy. Expression of miR-374c-5p and Fas mRNA by RT-qPCR, and Fas protein in HUVECs by was detected by Western blot. Results Proliferation of HUVECs was significantly inhibited(P＜0.001);H₂O₂ was significantly increased as compared with the H₂O₂ intervention group(P＜0.001);Proliferation in H₂O₂ + miR-374c-5p inhibitor transfection group was significantly increased as compared to H₂O₂ intervention group(P＜0.001). Conclusions miR-374c-5p protectes the HUVECs against apoptosis induced by H₂O₂.

Key words: miR-374c-5p, human umbilical vein endothelial cells(HUVECs), hydrogen peroxide(H₂O₂), oxidative stress, apoptosis

中图分类号:

甲宗虎, 晋群, 韩淑芳, 胡毓洪, 任昌振, 李云萍, 刘文燕. miR-374c-5p降低过氧化氢诱导的人脐静脉内皮细胞凋亡[J]. 基础医学与临床, 2025, 45(11): 1457-1462.

JIA Zonghu, JIN Qun, HAN Shufang, HU Yuhong, REN Changzhen, LI Yunping, LIU Wenyan. miR-374c-5p reduces hydrogen peroxide induced apoptosis of human umbilical vein endothelial cells[J]. Basic & Clinical Medicine, 2025, 45(11): 1457-1462.

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miR-374c-5p降低过氧化氢诱导的人脐静脉内皮细胞凋亡

miR-374c-5p reduces hydrogen peroxide induced apoptosis of human umbilical vein endothelial cells

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