Effects of 5-Aza-induced BM-MSCs transplantation on myocardial infarction area, related cytokines and potassium ion channels in rats
LI Ming-yang, WANG Shu-xia, ZHAO Jing-miao, WANG Qiu-ping, CHEN Ping, JIN Hua, HU Ji-hong
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Objective To observe the effects of 5-azacytidine(5-Aza)-induced bone marrow mesenchymal stem cells(BM-MSCs) transplantation on the myocardial infarction area, the expression of related cytokines and potassium channels in rats. Methods Rats were randomly divided into control group, model group, blank control group, BM-MSCs group and 5-Aza-BM-MSCs group. Coronary artery ligation was used to develope a model of myocardial infarction, and cell transplantation was performed using a 4-point injection method. HE staining was used to observe the pathology of infarct myocardium, and the expression of caspase-3, beclin-2, VEGF, PGI2, superoxide dismutase (SOD), IL-3, IL-6 and TNF-α in infarct areas were detected by immunohistochemistry. The Kv1.2 and Kv1.5 proteins expressions of potassium channels were detected by Western blot. Results 1)Compared with the control group, the area of myocardial infarction and the expression level of caspase-3, beclin-2, VEFG, PGI2, IL-3, IL-6 and TNF-α in the model group all significantly increased (P＜0.05). However, the expression level of Kv1.2 and Kv1.5 were reduced (P＜0.05). 2)Compared with the model group, the area of myocardial infarction and caspase-3, beclin-2, and IL-3, IL-6 and TNF-α expression level significantly reduced both in the BM-MSCs group and the 5-Aza-BM-MSCs group (P＜0.05), Kv1.5, VEGF, PGI2, and SOD expression level increased(P＜0.05); Kv1.2 in BM-MSCs group decreased, but increased in 5-Aza-BM-MSCs group (P＜0.05). 3)Compared with the BM-MSCs group, the area of myocardial infarction and the expression of caspase-3 and IL-6 decreased in the 5-Aza-BM-MSCs group (P＜0.05), Kv1.2, VEGF and SOD expression level increased (P＜0.05). Conclusions 5-Aza-BM-MSCs more effectively reduces inflammatory response and apoptosis, promotes neovascularization, improves myocardial antioxidant capacity, reduces myocardial infarction area, and promotes the expression of potassium ion channel Kv1.2 protein as compare to single BM-MSCs transplantation.