Basic & Clinical Medicine ›› 2021, Vol. 41 ›› Issue (1): 68-71.

• Original Articles • Previous Articles     Next Articles

Effects of main compositions of Astragalus on estradiol synthesizing activing in TGF-β1-induced rat testicular Leydig cell line

LI Yan-rong1*, LIU Guang-wei1, ZHAO Jing2, LI Ya-jun1   

  1. 1. Gansu University of Traditional Chinese Medicine(Dingxi Campus), Dingxi 743000;
    2. Department of Clinical Laboratory, Dingxi People's Hospital, Dingxi 743000, China
  • Received:2019-04-06 Revised:2020-09-30 Online:2021-01-05 Published:2020-12-30
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Abstract: Objective To investigate the effects of the main compositions of Astragalus on testicular Leydig cells and their synthesis of estradiol(E2), P450arom under TGF-β1 induction. Methods Leydig cells were divided into three groups: control group, model group and Chinese herbal medicine intervention group. Results Compared with the control group, Leydig cells in the model group showed abnormal morphology and less cells(P<0.01).TGF-β1 significantly inhibited the synthesis of E2 and P450arom by Leydig cells (P<0.05). Total Astragalus flavone, total Astragalus saponin and total astragalus polysaccharide all resulted in pathological changes of Leydig cells induced by TGF-β1. Compared with the model group, the number of cells increased significantly (P<0.01) and all of them alleviated the inhibitory effect of TGF-β 1 on the synthesis of E2 in Leydig cells induced by TGF-β1, and the effect of concentration of 20 mg/L total saponin of Astragalus was the most significant. Conclusions Total Astragalus saponin is the most effective component in Astragalus to exert TGF-β1 inhibitory effect on E2 synthesis by Leydig cells.

Key words: Astragalus, Leydig cells of testis, estradiol(E2)

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