Table of Content

    05 October 2012, Volume 32 Issue 10
    How residents improve bedside teaching
    2012, 32(10):  1115-1117. 
    Asbtract ( 815 )   PDF (405KB) ( 763 )  
    Related Articles | Metrics
    Abstract: Resident doctors play very important role in clinical teaching in teaching hospitals. They contribute significantly to the quality of undergraduate medical clerkship training and it is assumed that by participating in the process, they also improve their own professional competency. Residents’ clinical teaching should focus on training clinical skills, good clinical habits, patient-doctor communications, and professionalism. Learning clinical teaching skills will help residents enjoy teaching and improve teaching quality.
    Promoter methylation analysis of clock genes in mice peripheral tissues
    2012, 32(10):  1118-1125. 
    Asbtract ( 1092 )   PDF (2432KB) ( 453 )  
    Related Articles | Metrics
    Abstract: Objective To establish a simple, rapid and inexpensive method to determine the methylation status of eight key clock genes, BMAL1, BMAL2, CLOCK, NPAS2, PER1, PER2, CRY1 and CRY2. To examine the methylation of clock promoters in five peripheral tissues with the developed method. Methods Genome DNA was deaminated by Sodium metabisulfite solution and hydroquinone. The two sets of PCR primers for unmenthylated and methylated DNA, respectively, were used to amplify the promoter of clock genes. Polymerase chain reaction (PCR) products were then loaded and electrophoresed on 3% agarose gels. The PCR products for each reaction were sequenced directly. Results Specific amplicons with correct size were amplified. PCR products were also confirmed by sequencing. Conclusion In the present study, a new method of detection of the methylation of clock genes was successfully established; it may apply a new technique for clock genes promoter methylation detection. All of the clock promoters are free from methylation in the adult mouse.
    p38 MAPK Inhibitor decreases the TNF-α level in Spinal Cord of Rats with Chronic Constriction Injury
    2012, 32(10):  1126-1131. 
    Asbtract ( 876 )   PDF (1080KB) ( 675 )  
    Related Articles | Metrics
    Abstract:Objective: In this study, we examined the hypothesis that chronic constrictive injury (CCI) of the sciatic nerve could promote spinal cord release of TNF-α and produce allodynia via the p38 MAPK pathway. Methods: SD rats were divided into five groups: 1) control rats, 2) sham surgery rats, 3) CCI surgery rats without treatment, 4) CCI surgery rats with saline treatment, and 5) CCI surgery rats with the p38 inhibitor SB203580 treatment. In treatment groups, saline or SB203580 was given intrathecally starting 1 day before or 1 day or 7 days after CCI. All rats were killed at different times after surgery to examine p38 activity and TNF-α levels in the spinal cord by Western blot analysis or immunohistochemistry. Mechanical allodynia was tested by a series of von Frey hairs 3, 7, and 14 days after surgery. Results p-p38was significantly increased at 3, 7, and 14 days after CCI surgery compared with time-matched shams (P <0.05). Peripheral nerve injury induced mechanical allodynia and enhanced spinal concentrations of TNF-α (P<0.05). Pretreatment or early treatment with SB203580 inhibited p38 activity, resulting in reduction of TNF-α synthesis and attenuation of mechanical allodynia (P< 0.05). Conclusion: p38 activation is one aspect of the signaling cascade that culminates in TNF-α synthesis and contributes to mechanical allodynia after peripheral nerve injury.
    Effect of Poly(ADP-ribose) glycohydrolase (PARG) gene silencing on liver metastasis of colorectal carcinoma CT26 cell line in mice
    2012, 32(10):  1132-1136. 
    Asbtract ( 1234 )   PDF (823KB) ( 671 )  
    Related Articles | Metrics
    Abstract:Objective This study was to investigate the effect of PARG-shRNA on liver metastasis of colorectal carcinoma CT26 cell line in mice. Methods Mice were divided at random into three groups. Animal models for Liver metastases of colorectal cancer were established by intrasplenic inoculation of colorectal carcinoma cell in BALB/c mice.CT26 cells transfected with empty vector and CT26 cells transfected with PARG-shRNA were inoculated to spleen caspsule.CT26 cells transfected with empty vector and untransfected CT26 cells served as control. The change of spleen and liver metastases carcinoma nodules were observed and counted. The expressions of PARG, PARP, NF-κB, integrin-β1,MMP-2,MMP-9 in spleen transplant tumor were measured by Western blot analysis. Results The size of spleen transplant tumor and liver metastatic nodules in transfected group were smaller than that in the control groups (p<0.05). The expression of PARG(0.0105±0.0028)、PARP(0.1786±0.024)、NF-κB(0.1678±0.0359)、integrin-β1、MMP-2、MMP-9 in transfected group was weaker than that in the control groups (p<0.05). Conclusion The growth of spleen transplant tumor and liver metastases can be inhibited by PARG gene silencing in CT26 cells. It is probably through inhibiting PARP, NF-κB and NF-κB-dependent gene downstream.
    Effect of hypoxia on SW480 colon cells and the relationship between hypoxia and autophagy
    2012, 32(10):  1143-1148. 
    Asbtract ( 1146 )   PDF (945KB) ( 758 )  
    Related Articles | Metrics
    Abstract: Objective To observe the effects of hypoxic microenvironment on the proliferation, invasion and autophagy of colon cancer cells and investigate the relationship between survival and autophagy of colon cancer cells under hypoxic conditions. Methods: CD 133+ cancer stem cells (cancer stem cells, CSCs) were purified using MACS (magnetic activated cell-sorting system) CD 133+ cell isolation kit. The invasion of colon cells was measured by Boyden Chamber. The ultrastructure was observed using transmission electron microscope (TEM). The autophagy vesicles were detected by monodansylcadaverin (MDC) fluorescence. The relationship between autophagy and cell survival was analyzed using flow cytometry together with trypan blue staining. Results In Boyden Chamber, the amount of SW480 moving into the second floor was increased under the hypoxic microenvironment. In experimental groups that were exposed to hypoxic conditions, the MDC fluorescence intensity of SW480 cells decreased from 20.53±0.64 to 17.00±0.20, while that of CD133+ CSCs increased from 19.89±0.58 to 33.13±1.95 (P<0.05). The trypan blue staining showed that under hypoxic conditions, the viability of SW480 cells decreased significantly over time (P<0.05), while that of CD133+ cells had no significant changes. Conclusion: For SW480 cells, the invasion could be enhanced under the hypoxic microenvironment, with both the level of autophagy and viability decreased. For CD133+ cells, the occurrence of autophagy increased under the hypoxic environment, with the viability basically unchanged.
    IL-1 increases ACAT-1 expression and activity of foam cells by protein kinase C /mitogen activated protein kinase pathway
    2012, 32(10):  1149-1153. 
    Asbtract ( 1222 )   PDF (697KB) ( 786 )  
    Related Articles | Metrics
    Abstract Objective Our current investigation attempts to study whether interleukin-1 (IL-1) increases foam cells acyl-CoA cholesterol ester acyltransferase-1 (ACAT-1) expression and activity by protein kinase C (PKC)/mitogen-activated protein kinase (MAPK) signaling pathway. Methods The mononuclear cells (THP-1) were co-cultured with phorbol myristate acetate (PMA) and induced into differentiation to macrophages. The macrophages were cultured with oxidized low density lipoprotein (Ox-LDL) and induced into differentiation to the foam cells. Oil red O staining was employed to examine cytoplasm lipid deposition. PKC and MAPK activity was detected in three different groups (the foam cells group, the foam cells plus IL-1 group, the foam cells plus IL-1/IL-1 monoclonal antibody group). PKC and MAPK inhibitors were separately added to the three different groups. Western blot assay and liquid phase scintillation counting were employed to examine the expression of ACAT-1 protein and ACAT-1 activity in different groups, respectively. Results THP-1 cells presented a suspension growth pattern, and showed significant changes including the adhensive growth, ovalisation shape after cultured in RPMI l640 media containing 200nM PMA for 48h. Some red stained substance could be found in the cytolymph after co-cultured with 100mg/L Ox-LDL for 24h. The PKC and MAPK activities in the the foam cells plus IL-1 group are higher than those in the foam cells group (P<0.05). The expression and activity of ACAT-1 in the the foam cells plus IL-1 group are higher than those in the foam cells group (P<0.05). After added PKC and MAPK inhibitors, the up-regulation effects of IL-1 on the expression and activity of ACAT-1 were remarkably decreased (P<0.05). Conclusions PKC/MAPK signaling pathway could be involved in the IL-1 up-regulation effects on the ACAT-1 expression and activity in the the foam cells.
    AMPK expression decreases in alcoholic liver disease
    2012, 32(10):  1154-1160. 
    Asbtract ( 846 )   PDF (2899KB) ( 852 )  
    Related Articles | Metrics
    【Abstract】 Objective To observe the effects of ethanol consumption on the expression of AMP-activated protein kinase(AMPK) in the rats hepatic tissue with alcoholic liver disease(ALD). Methods In the 50 male Wistar rats, 10 rats were randomly assigned to the normal control group. Others were to develop the rats model of ALD by intragastric alcohol and sacrificed randomly at the end of 4th, 8th, 12th and 16th week, and the serum and liver samples were collected respectively. The contents of ALT, AST, CHE, TG, TC, LDL, VLDL, HDL in serum were examined and the pathological changes were observe in liver tissue by HE, Sirius red.and Sudan Ⅳ. And the protein expression of AMPK, Acetyl-CoA carboxylase (ACC), Sterol regulatory element-binding proteins (SREBP) and mRNA were detected by immunohisto-chemical staining and RT-PCR respectively. Results With the consumption of ethanol , the level of ALT and AST in the serum increased while the CHE decreased gradually. The level of TG, TC, LDL, VLDL in the serum increased while the HDL decreased gradually. Compared with the control group, the expression of AMPK in model group decreased gradually with the progress of ALD, and it negatively correlated with the expression of ACC and SREBP of hepatic tissue(r=-0.911and -0.907 respectively, P<0.01). Conclusions The expression of AMPK in hepatic tissue was decreased with the process of ALD, which inhibit the activation to ACC and SREBP. It causes the increase of lipid synthesis, which would result in the fat accumulation in the liver and has a great effect on the liver injury. As a new pharmacological target, AMPK provides a new idea for the prevention and treatment of ALD.
    Newcastle disease virus infect activated human hepatic stellate cells LX-2
    2012, 32(10):  1161-1166. 
    Asbtract ( 1487 )   PDF (1181KB) ( 540 )  
    Related Articles | Metrics
    Objective To explore the replication efficiency of Newcastle disease virus in hepatic stellate cells. Methods Human hepatic stellate cells LX-2 were stimulated with TGF-β1 and the proliferation of LX-2 cells was detected by MTT assay, RT-PCR was used to measure the changes of related genes mRNA levels. Different passages LX-2 cells and primary mouse HSCs with or without TGF-β1 stimulation were infected by NDFLtag-EGFP, NDV replication in these cells was observed under fluroscent microscopy. The replication of NDFLtag-EGFP and NDV-Italien in LX-2 was detected by flow cytometry (FACS). Results TGF-β1 stimulated the activation of LX-2 cells. The efficiency of NDV replication was increased with the consecutive passages (from (15.65±0.92)% to (23.05±1.5)%, (P<0.05)) and TGF-β1 stimulation of LX-2 cells (from (12.8±1.4)% to (22.7±1.7)%, (P<0.05)). In primary isolated mouse HSCs, NDV also had increasing replication with HSC passage and TGF-β1 stimulation. Conclusions NDV replicate in activated HSC effectively, it means that activation of HSC facilitate the replication of NDV in this kind of cells.
    The relation of serum squamous cell carcinoma antigen level, pelvic lymph node HPV16/18 infection in patients with cervical squamous cell carcinoma and its prognosis
    2012, 32(10):  1167-1170. 
    Asbtract ( 1105 )   PDF (404KB) ( 625 )  
    Related Articles | Metrics
    Abstract: Objective the study aimed to explore the relation of preoperative serum SCCA levels, pelvic lymph node HPV16/18 infection in patients with cervical squamous cell carcinoma and its prognosis. Methods ELISA was performed to detect the SCCA level and real-time fluorescence quantitative PCR was utilized to analyze the pelvic lymph node HPV16 / 18 positive rate. The relation of SCCA level, HPV16/18 positive rate and the three-year survival rate was investigated. Results The SCCA level is closely related with the infection of HPV16/18 in pelvic lymph node (P<0.05).The media follow-up time for 35 patients with cervical squamous cell carcinoma was 36 m(rang 12 m-41 m). Four patients were dead and the overall 3 year survival rate was 85.0%. The 3 year survival rates for patients with positive SCCA and negative SCCA were 100% and 69.2% respectively and that were 96.4% and 57.1% for patients with positive pelvic lymph node HPV16/18 and negative pelvic lymph node HPV16/18. the 3 year survival rates for patients with negative SCCA and lymph node HPV16/18 were obviously better than that of positive ones(P<0.05). lymph node metastasis, SCCA level and pelvic lymph node HPV16/18 infectionstatus were associated with prognosis. Conclusion SCCA level and pelvic lymph node HPV16/18 infection status were associated with the prognosis of cervical squamous carcinoma patients and could be the prognostic markers for cervical squamous carcinoma.
    Matrix metalloproteinases-1,-2 expression in vaginal tissues from women with pelvic organ prolapse
    2012, 32(10):  1171-1174. 
    Asbtract ( 1049 )   PDF (517KB) ( 745 )  
    Related Articles | Metrics
    Objective Compare the expression of Matrix metalloproteinase (MMP)-1 and -2 in the vaginal tissue from 20 women with pelvic floor dysfunction and the controls in order to investigate the relation of Matrix metalloproteinase and female pelvic floor dysfunction. Methods A total of 40 women referred for pelvic reconstruction surgery for pelvic floor dysfunction or benign gynaecological disease were included in this study from January 2009 to May 2010. During surgery, vaginal tissue biopsies were obtained from patients with POP (n=20) and women without evidence of pelvic floor weakening (n=20). Level of MMP-1, -2 and pro-MMP-2 in the vaginal tissue of women were measured. Statistical evaluations were made by Student t-test or x2 test. Results A significant increase in MMP-1 expression was seen in vaginal tissue from women with POP (p<0.05). In contrast, there was no difference in the expression of MMP-2 between women with POP and those without (p>0.05). Interesting, a significant increase in pro-MMP-2 expression was seen in vaginal tissue from women with POP (p<0.05). Conclusions MMP-1 increases the collagen degradation. Increased MMP-1 expression in the vaginal tissue is associated with urogenital prolapse.
    Clinical significance and expression of ANO1 in oral squamous cell carcinoma
    2012, 32(10):  1175-1178. 
    Asbtract ( 1716 )   PDF (765KB) ( 772 )  
    Related Articles | Metrics
    Objective To study the clinical significance of ANO1 mRNA and protein in oral squamous cell carcinoma (OSCC) and to find the biological foundation of making a proper treatment for OSCC. Methods The specimens of 81 patients with OSCC were examined by using SP immunohistochemistry and northern blot.The expression of ANO1 protein and clinical and pathological feature were determined and analyzed.The expression of ANO1 protein in head and neck cancer cell lines were examined by western blot.Results ANO1 mRNA and protein expression were significantly higher in OSCC than those in normal oral tissue(P<0.05), and showed significant correlation with clinical stage of OSCC (P<0.05) and lymph node metastasis (P<0.05). No statistical significance was observed in of the ANO1 expression in histological grades and patient sex, age (P>0.05). The lowest endogenous ANO1 was expressed in Hep-2 cell line. The most endogenous ANO1 was expressed in SCC-25 cell line. Conclusion ANO1 may be involved in the occurrence, metastasis and progress of OSCC. Analysis of the expression characteristics of ANO1 and clinic and pathological features of OSCC may better conduct clinical therapy of OSCC.
    The expression of FSHR and LHR in ovariectomized rat kidney
    2012, 32(10):  1179-1182. 
    Asbtract ( 936 )   PDF (545KB) ( 596 )  
    Related Articles | Metrics
    Objective By studying the characteristics of FSH receptor (FSHR), LH receptor (LHR) in rat renal tissue, further to prove the similar features and changes in renal and ovary, then to reveal the substantial of " The renal governs reproducion " ,to provide the experimental basis for the theory of traditional Chinese medicine, and to add new contents for the physiological functions of the renal. Methods To prepare the model of ovariectomized rats, then to study the characteristics of FSHR, LHR in normal and ovariectomized rat kidney tissue by radioimmunoassay, RT-PCR semi-quantitative methods. Results The two-phase adjustments of FSH, LH receptor in kidney and ovary is same: low doses of FSH, LH increased the level of the FSHRmRNA, LHRmRNA, while high doses of FSH, LH down the level of the FSHRmRNA, LHRmRNA; the treatment of high-dose estrogen significantly decreased the level of the FSHRmRNA, while the death of the fetal kidney cell suspension effectively increased the level of the E2 in ovariectomized rats, reduced the expression of FSHR mRNA. Conclusion The regulation characteristics of the FSHR,LHR was same in rat kidney and ovarian tissues, kidney and ovarian tissues may have similar functions, to participate in the process of the synthesis and regulation of hormone.
    Effect of shRNA targeting VEGF on human skin squamous carcinoma cell line A431
    2012, 32(10):  1183-1187. 
    Asbtract ( 907 )   PDF (1511KB) ( 723 )  
    Related Articles | Metrics
    【Abstract】Objective The eukaryotic expression plasmids targeting VEGF (psilencer-VEGF1-shRNA,VEGF-s1;psilencer-VEGF2-shRNA,VEGF-s2)were constructed and transfected these plasmids to the human skin squamous cell carcinoma cell line (A431) to observe its affect to a series of biological characteristics of A431 cells for exploring the significance of intervention by VEGF-s1 and VEGF-s2. Method: VEGF-s1,VEGF-s2 and random target sequence of the negative control plasmid (psilencer-Target-off-shRNA,T-off)were constructed, simultaneously. and transfected these plasmids to A431 by Tm2000. The expression of VEGF mRNA and protein were detected by RT-QPCR, western blot and ELISA; the vitality of A431 cells was examined by CCK-8 assay; the percentage of Cell Proliferative Cycle and apoptosis of A431 were tested by flow cytometry; the migration capacity of A431 in two-dimensional and three- dimensional space were inspected by wound healing effect and Transwell assay,respectively; the adhesion potential of A431 was detected by FN adhesion test. Results: After VEGF-s1 or VEGF-s2 treatment, cytoactivity declined, cell cycle arrested, G1 cell proportion increased sharply, S cell proportion decreased obviously, apoptosis increased, the expression of VEGF mRNA and protein decreased significantly, the migration and adhesion of A431 cells was suppressed significantly, as compare with the control group and untreated cells(every ,p<0.05). Conclusion: VEGF is an important growth-related gene of human skin squamous cell carcinoma, silencing VEGF could effectively inhibit the biological behavior of A431 cells.
    Effect of high fructose-induced hypertriglyceridmia on kidney of healthy rats
    2012, 32(10):  1188-1193. 
    Asbtract ( 900 )   PDF (1335KB) ( 615 )  
    Related Articles | Metrics
    [Abstract] Objective To investigate the effect of high fructose-induced hypertriglyceridmia on kidney of healthy Wistar rats. Methods Thirty-two healthy male Wistar rats were randomly divided into two groups (n =16): normal control group and high fructose group. Rats were sacrificed by the end of 8 and 16 weeks. BUN, Scr, fast glucose ,lipid concentration, urinary microalbumin were observed. Triglyceride of renal cortices were detected. The pathological changes were examined by PAS staining. IV-type collage and α-SMA protein were examined by immunohistochemistry staining. The electron microscope sections were made to measure GBM width Results By the end of 8 and 16 weeks, compared with the control group, high fructose group showed elevated triglyceride (1.65+0.86)mmol/L、(2.13+0.87)mmol/L(both P<0.05 ), and very low density lipoprotein chloesterol(0.75+0.39)mmol/L、(0.97+0.40)mmol/L(both P<0.05 ) in blood. Renal cortices were both significantly increased(7.21+2.20)mg/g、(7.92+3.05)mg/g (both P<0.05). The urinary microalbumin significantly increased too(63.00+12.00)μg、(150.00+48.00) μg (both P<0.05). The pathological changes were serious in the high fructose group. The expression of IV-type collagen and α-SMA protein were up-regulated. Electron microscope revealed GBM obviously thickened in high fructose group. Conclusion High fructose-induced hypertriglyceridmia may cause lipid nephrotoxicity in Wistar rats.
    Relationship Between the Genetic Polymorphism of GSTT1 Gene 、smoking and Lung Cancer Susceptibility
    2012, 32(10):  1194-1197. 
    Asbtract ( 1054 )   PDF (387KB) ( 665 )  
    Related Articles | Metrics
    Objective: To investigate the association between the genetic polymorphism of GSTT1 and the susceptibility to lung cancer and a synergetic interactions between smoking and genetic polymorphisms on lung cancer susceptibility.Methods: A case-control study and PCR—RFLP techniques were used to identify GSTT1 genotype in 100 cases of lung cancer and 135 controls.Results: The frequency of GSTT1(-) genotype in lung cancer group and controls was 57%(57/100) and 41.5%(56/135) respectively(P=0.016). There were no gender differences of GSTT1 (-) genotype distribution. In people with GSTT1(-) genotype ,smoking markedly increased the risk for lung cancer(P=0.016).In smokers,GSTT1 (-) genotype significantly increased the risk for lung cancer(P=0.006).In smokers≥ 20 packyears,GSTT1 (-) genotype remarkably increase the risk of lung cancer (P=0.002).Conclusions:The distribution frequency of GSTT1(-) genotype was higher in lung cancer,which Might increase the genetic susceptibility to lung cancer.There might be a synergetic interaction between smoking and GSTT1(-) genotype.
    Grape seed procyanidin decreases the acute chemical hepatic injury in mice
    2012, 32(10):  1198-1201. 
    Asbtract ( 1007 )   PDF (631KB) ( 560 )  
    Related Articles | Metrics
    Background: Even though there are so many researches on grape seed procyanidin, its protective effect on chemical hepatic injury in mouse has seldom been explored. Objective:To investigate the protective effect of grape seed procyanidin on chemical hepatic injury in mouse Methods:Copy acute chemical hepatic injury in the mouse with alcohol and carbon tetrachloride respectively as the model group; treat the mouse with different concentrations of grape seed procyanidin (GSP) by intragastric administration as the treatment group; then detect the serum concentration of alanine aminotransferase (ALT) and aspartate aminotransferase (AST); analyze the content of superoxide dismutase (SOD) and malondialdehyde (MDA) and observe the pathological change in the hepatic tissue. Results:Two types of acute chemical hepatic injury model were obtained successfully and all index in the model groups differed from the control group significantly (P<0.01). Compared with the model groups, the content of MDA and serum concentration of ALT and AST in the treatment group decreased dose-dependently (P<0.05), but SOD increased in a dose-dependent way. Conclusions:GSP has some protective effect on the alcohol or carbon tetrachloride-induced chemical hepatic injury in the mouse. To some extent, GSP protects the liver in a dose-dependent way.
    Expression and clinical pathology significance of p53,ki-67, galectin-3, HBME-1, 34βE12 and CK19 in papillary thyroid carcinomas
    2012, 32(10):  1202-1206. 
    Asbtract ( 1098 )   PDF (639KB) ( 684 )  
    Related Articles | Metrics
    [Abstract] Objective: To investigate the expression of p53,ki-67, galectin-3, HBME-1, 34βE12 and CK-19 in the papillary thyroid carcinomas and clinical pathology significance. Methods: By the s-p immunohistochemical technique, the expression of p53,ki-67, galectin-3, HBME-1, 34βE12 and CK-19 was detected in 43 cases of papillary thyroid carcinomas, 37 multinodular goiters, 33 follicular adenomas and 17 Hashimoto’s thyroiditis. Results: The expression rate of p53,ki-67, galectin-3, HBME-1, 34βE12 and CK19 in the papillary thyroid carcinomas was 88.37%, 79.07%, 88.37%, 93.02%, 86.05%and 95.35% respectively; Expression of p53,ki-67, galectin-3, HBME-1, 34βE12 and CK-19 were obviously different in the papillary thyroid carcinomas and benign lesions ( P<0.01). Conclusion: Combined application of immunohistochemical staining of p53,ki-67, galectin-3, HBME-1, 34βE12 and CK-19 is a more useful option to distinguish the papillary thyroid carcinoma from benign lesions.
    Role of β-catenin in mice hair follicle senescence
    2012, 32(10):  1207-1211. 
    Asbtract ( 967 )   PDF (1131KB) ( 595 )  
    Related Articles | Metrics
    Abstract:Objective In C57 young mice and 34-month-old senile mice, β-catenin expression in hair follicle was compaired between the two age groups to investigate the relationship between β-catenin and mice hair follicle senescence. Methods The expression pattern of β-catenin in hair follicles of two age groups of C57 mice was examed by immunofluorescence, RT-PCR, and Western blot. Results Compaired to young mice, both β-catenin mRNA and protein expression levels were upregulated in old mice hair follicle at various stages of hair cycle. In the anagen skin of 34months old mice, β-catenin protein expressions were at the level of 0.9024±0.0167, significantly higher then the level in the anagen skin of 35 days old mice(0.8012±0.0184). In the telogen skin of 34months old mice, β-catenin protein expressions were 0.6348±0.0241, significantly higher then the level in the telogen skin of 23 days old mice(0.6348±0.0241)(P<0.05).β-catenin protein distribution was localized at the up-portion of outer root-sheath and Bulge cells, while the nuclear expression was obviours. No apparente difference of the β-catenin expression pattern was observed in various stages of hair cycle in senile mice.Conclusion Compaired to young mice, in senile mice hair follicle, the changes of β-catenin expression pattern, including the expression level, periodicity and localization, may contribute to the senescent poliosis.
    Hemodialysis patients serum induce apoptosis of HUVECs
    2012, 32(10):  1212-1215. 
    Asbtract ( 991 )   PDF (710KB) ( 590 )  
    Related Articles | Metrics
    Objective To validate the apoptosis of HUVECs induced by hemodialysis patients seum and explore the mechanism.Methods HUVECs are exposed to culture medium containing serum obtained from patients undergoing haemodialysis(trial group) and healthy volunteers(control group).The effect of uraemic serum on the apoptosis of endothelial cells in vascular networks are observed under light-microscope;Cell proliferation are measured by MTT;Apoptotic cells in vascular networks are evaluated by TUNEL;To investigate the expression of caspase-3 and production of ROS, cell lysates are evaluated by western blotting and spectrophotometry.Results After HUVECs are exposed to pooled serum medium for 40 h, there are apoptotic endothelial cells in vascular networks (P<0.05) and the endothelial cells produce more ROS in trial group(P<0.05).At 40h, the expression level of caspase-3 in endothelial cells in trial group is(0.83±0.03),significantly higher than in control group(0.57±0.02)(P<0.05).Conclusion Hemodialysis patients serum can induce apoptosis of HUVECs in vascular networks. Higher expression of caspase-3 and more production of ROS in endothelial cells may be involved in the mechanism.
    Fever and dyspnea after autologous peripheral blood stem cell transplantation
    2012, 32(10):  1216-1220. 
    Asbtract ( 1403 )   PDF (900KB) ( 598 )  
    Related Articles | Metrics
    Engraftment syndrome (ES) is a severe complication that occurs during neutrophil recovery following hematopoietic stem cell transplantation. It is characterized by fever, skin rash, and non-cardiac pulmonary edema. Previously, ES was associated with a high transplant-related mortality, mostly from respiratory failure and associated multi-organ dysfunction syndrome. For ES, early diagnosis and corticosteroid therapy can dramatically decrease transplant-related mortality rate. The authors report a young female patient with Non Hodgkin Lymphoma who presented with fever, dyspnea and diffuse pulmonary infiltration at the time of neutrophil engraftment following autologous peripheral blood stem cell transplantation. She was early diagnosed as ES and recovered promptly after treatment with corticosteroid.
    The inhibitory effect of specific small interfering RNA against nucleus accumbens-1 gene on cell proliferation of ovarian cancer cells
    2012, 32(10):  1221-1223. 
    Asbtract ( 1409 )   PDF (508KB) ( 591 )  
    Related Articles | Metrics
    Objective: To investigate the effect of specific small interfering RNA (siRNA) against nucleus accumbens-1(Nac1 or NAC-1)gene on the proliferation of ovarian cancer cell line HO8910. Methods: Three specific NAC-1 siRNAs and one negative siRNA were individually transfected into HO8910 cells for 48 h . The expression levels of NAC-1 mRNA and protein were analyzed by real-time fluorescence quantitative PCR (qRT-PCR) and Western blotting, respectively. The cell proliferation was determined by MTT assay and plate clone formation assay. The distribution of cell cycle was assessed by flow cytometry. Results: In comparison with the other two kinds of NAC-1 siRNAs, NAC-1-siRNA-1 was demonstrated to have the strongest silencing effect on NAC-1 gene expression in HO8910 cells at 48 h after transfection. The expression levels of NAC-1 mRNA and protein were reduced by 65% and 80%, respectively. The growth rate of HO8910 cells transfected with NAC-1-siRNA-1 was significantly decreased. Moreover, the cell cycle arrest in the G1 phase was observed when HO8910 cells were transfected with NAC-1-siRNA-1 for 48 h. Conclusion: Specific NAC-1 siRNA can effectively inhibit the expression of NAC-1 gene and significantly suppress cell proliferation of ovarian cancer HO8910 cells.
    Protein and gene dynamic expressions of leptin, collagen I and collagen III in the tissues of hepatic fibrosis model
    2012, 32(10):  1224-1226. 
    Asbtract ( 1605 )   PDF (289KB) ( 597 )  
    Related Articles | Metrics
    Abstract: Objective To investigate protein and gene dynamic expressions of leptin, collagen I and collagen III in the tissues of hepatic fibrosis model. Methods Liver fibrosis models of rats were made by hypodermic injection with 60% CCl4. The protein and gene expressions of leptin, collagen I and collagen III were assayed by Western blot and reverse transcription polymerase chain reaction(RT-PCR), respectively. Results The protein and gene expressions of leptin, collagen I and collagen III were slight in normal liver tissues. In the model groups, the expressions began to increase progressively at the end of the 2nd week after injection with CCl4. The mRNA expression levels of leptin, collagen I and collagen III in the model groups were higher than that in the control group (P<0.05), and there were closely positive correlation between leptin and collagen I(r=0.595, P=0.017), collagen III (r=0.478, P=0.011).Conclusion In the process of hepatic fibrosis induced by CCl4 injection in rats, expression of leptin and collagen I, III are increased with the development of hepatic fibrosis. Leptin probably participate the synthesis and degradation of extracellular matrix in hepatic fibrosis.
    Research progresses on lipotoxicity and islet microvascular endothelial cells injury
    2012, 32(10):  1227-1229. 
    Asbtract ( 1091 )   PDF (445KB) ( 617 )  
    Related Articles | Metrics
    Abstract Islet microcvascular endothelial cells (IMECs) ,as themost pivotal part of microcirculation tissue barrier in islet , plays a critical role in islet endocrine function and pathogenesis of diabetes.It is well established abnormal glucose and lipid metabolism coexist in type 2 diabetes .Excessive lipotoxicity can cause IMECs injury and dysfunction directly by inducing oxidative stress,vasomotor dysfunction and cell apoptosis.In this review, we will describe the functional characteristics of IMECs and the possible mechanisms involved in the lipotoxicity induced cellular injury and dysfunction.
    Internal sequence binding characteristics of PDZ domain and clinical applications
    2012, 32(10):  1230-1234. 
    Asbtract ( 1099 )   PDF (569KB) ( 595 )  
    Related Articles | Metrics
    PDZ domains are abundant protein interaction modules that mediate multiple biological processes and physiological functions. They recognize canonical four to five amino acids at the C-termini of partner proteins. However, there are probably more patterns of protein interaction than we currently know. More and more studies showed that PDZ domains also have non-canonical binding specificities. Researches on the non-canonical binding specificities of PDZ domains can help to enrich the protein interaction networks and discover novel functions of proteins, and will be benefit for development of save and effective drugs for disease treatment.
    Research Advances in the function of USP22 in the occurrence and development of tumor
    2012, 32(10):  1235-1238. 
    Asbtract ( 912 )   PDF (415KB) ( 599 )  
    Related Articles | Metrics
    Abstract: USP22 is the ubiquitin-specific peptidase and a subunit of hSAGA complex subunit, belonging to the DUBs. Within hSAGA, USP22 deubiquitylates histone H2A and H2B and acetyltransfers H4 to regulate the transcription and expression of the related genes.Now that it is believed that USP22 is one of the death-from-cancer signature and associated with proliferation,invasion,metastasis and poor prognosis. It promotes the occurrence and development of tumor corresponding with the BMI-1,which is one of the Polycomb group, and Myc,FBP1 ,TRF1 .That makes it possible to become one of the tumor stem cell markers.