Abstract: Abstract: Objective the study aimed to explore the relation of preoperative serum SCCA levels, pelvic lymph node HPV16/18 infection in patients with cervical squamous cell carcinoma and its prognosis. Methods ELISA was performed to detect the SCCA level and real-time fluorescence quantitative PCR was utilized to analyze the pelvic lymph node HPV16 / 18 positive rate. The relation of SCCA level, HPV16/18 positive rate and the three-year survival rate was investigated. Results The SCCA level is closely related with the infection of HPV16/18 in pelvic lymph node (P<0.05).The media follow-up time for 35 patients with cervical squamous cell carcinoma was 36 m(rang 12 m-41 m). Four patients were dead and the overall 3 year survival rate was 85.0%. The 3 year survival rates for patients with positive SCCA and negative SCCA were 100% and 69.2% respectively and that were 96.4% and 57.1% for patients with positive pelvic lymph node HPV16/18 and negative pelvic lymph node HPV16/18. the 3 year survival rates for patients with negative SCCA and lymph node HPV16/18 were obviously better than that of positive ones（P<0.05). lymph node metastasis, SCCA level and pelvic lymph node HPV16/18 infectionstatus were associated with prognosis. Conclusion SCCA level and pelvic lymph node HPV16/18 infection status were associated with the prognosis of cervical squamous carcinoma patients and could be the prognostic markers for cervical squamous carcinoma.

Key words: cervical neoplasms, squamous cell carcinoma antigen, human papillomavirus, prognosis