Whole blood NPM1, MCP-1 and intestinal flora are associated with gastric cancer progression and prognosis

doi:10.16352/j.issn.1001-6325.2024.08.1137

Abstract

Abstract: Objective To investigate the association of whole blood nucleophosmin 1(NPM1), monocyte chemotactic protein-1 (MCP-1), and intestinal flora with gastric cancer progression and prognosis. Methods A total of 120 patients with gastric cancer who were admitted to the Nanyang Second People′s Hospital from May 2018 to May 2020 were selected as the gastric cancer group, 120 patients with benign gastric lesions underwent gastroscopy in the same period were selected as the benign gastric lesions group, and 120 patients with healthy physical examinations in the same period were selected as the control group. RT-qPCR was used to detect mRNA expression of NPM1 and MCP-1. The intestinal microbiota was examined by MicroScan microbial identification analysis system; The correlation between NPM1, MCP-1 and intestinal microbiota was analyzed by Pearson method. Multivariate COX regression was used to analyze the influencing factors of prognosis in gastric cancer patients. Results The level of NPM1 mRNA,exuberance of Bifidobacterium and Lactobacillus in the control group, gastric benign lesion group, and gastric cancer group decreased sequentially (P＜0.05), whereas MCP-1 mRNA, Enterococcus, and Escherichia coli increased sequentially (P＜0.05). Serum NPM1 mRNA, Bifidobacterium and Lactobacillus in stage Ⅲ-Ⅳ of gastric cancer were obviously lower than those found in stage Ⅰ-Ⅱ(P＜0.05), while MCP-1 mRNA, Enterococcus and Escherichia coli were obviously more than those found in stage Ⅰ-Ⅱ(P＜0.05). Pearson correlation analysis showed that, there was a negative correlation between serum NPM1 mRNA and MCP-1 mRNA levels in gastric cancer patients (P＜0.05), NPM1 mRNA was positively correlated with Bifidobacterium and Lactobacillus(P＜0.05) and negatively correlated with Enterococcus and Escherichia coli(P＜0.05). MCP-1 mRNA was negatively correlated with the quantity of Bifidobacterium and Lactobacillus(P＜0.05) but positively correlated with Enterococcus and Escherichia coli(P＜0.05). The level of NPM1 mRNA, quantity of Bifidobacterium and Lactobacillus in the poor prognosis group were obviously lower than those found in the good prognosis group (P＜0.05), while the level of MCP-1 mRNA, quantity of Enterococcus and Escherichia coli were obviously higher than those in the good prognosis group (P＜0.05). COX regression analyses showed that MCP-1 mRNA, Enterococcus and Escherichia coli were risk factors affecting the prognosis of gastric cancer patients (P＜0.05), while NPM1 mRNA, Bifidobacterium, and Lactobacillus were protective factors (P＜0.05). Conclusions Decreased level of NPM1, decreased counting of Bifidobacterium intestinalis and Lactobacillus, increased level of MCP-1, increased counting of Enterococcus and Escherichia coli are strongly associated with gastric cancer progression and poor prognosis.

Key words: nucleophosmin 1, monocyte chemotactic protein-1, intestinal microbiota, gastric cancer, prognosis

CLC Number:

R735.2

HOU Nan, LIU Yuan, GAO Jun, WANG Jing, YUAN Meng. Whole blood NPM1, MCP-1 and intestinal flora are associated with gastric cancer progression and prognosis[J]. Basic & Clinical Medicine, 2024, 44(8): 1137-1142.

References

[1] 朱娟, 王少明, 陈茹, 等. 胃癌筛查现状的研究进展[J].中华肿瘤杂志, 2020, 42: 603- 608.
[2] Sung H, Ferlay J, Siegel RL, et al. Global cancer statistics2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2021, 71:209-249.
[3] Karimi Dermani F, Gholamzadeh Khoei S, Afshar S, et al. The potential role of nucleophosmin (NPM1) in the development of cancer[J]. J Cell Physiol, 2021, 236: 7832-7852.
[4] Guo CA, Su XL, Wang WJ, et al. NPM1 is a diagnostic and prognostic biomarker associated with the clinicopathological characteristics of gastric cancer[J]. Neoplasma, 2022, 69: 965-975.
[5] 周静,陈萍,郑雅萍,等.感染性早产产妇血清MCP-1与sTNFR-Ⅰ和HMGB1及TNF-α的表达[J].中华医院感染学杂志, 2019, 29: 436-439.
[6] 黄箫娜,朱晨阳,李雨真,等.中药微调3号合剂对晚期胃癌免疫治疗临床疗效及肠道菌群的影响[J].中国医学科学院学报, 2023, 45: 581-590.
[7] 中华医学会消化内镜学分会, 中国抗癌协会肿瘤内镜专业委员会.中国早期胃癌筛查及内镜诊治共识意见(2014年,长沙)[J].中华消化内镜杂志, 2014, 31: 361-377.
[8] Wang S, Han H, Hu Y, et al.MicroRNA-130a-3p suppresses cell migration and invasion by inhibition of TBL1XRl-mediated EMTin human gastric carcinoma[J]. Mol Carcinog, 2018, 57: 383-392.
[9] Akimoto T, Muto S, Kutsuwada T, et al. Peritoneal dialysis andmalignancy: an experience with a patient complicated by gastriccarcinoma[J].Clin Med Insights Case Rep, 2019,12: 1179547619835176-1179547619835186.
[10] 曹小萌,夏天红,袁绍斌,等.核仁磷酸蛋白1在肿瘤中的研究进展[J].国际老年医学杂志, 2022, 43: 751-754.
[11] Wang S, Qin J, Ye H, et al. Using a panel of multiple tumor-associated antigens to enhance autoantibody detec-tion for immunodiagnosis of gastric cancer[J]. Oncoimmunology, 2018, 7: e1452582. doi: 10.1080/2162402X.2018.1452582.
[12] Dutta P, Sarkissyan M, Paico K, et al. MCP-1 is overexpressed in triple-negative breast cancers and drives cancer invasiveness and metastasis[J]. Breast Cancer Res Treat, 2018, 170: 477-486.
[13] 张元秋,曾萍,周彦生,等.血MCP-1、PCT、免疫球蛋白在甲状腺癌患者术后感染并发症预测中的价值分析[J].实用癌症杂志, 2021, 36: 1181-1185.
[14] Liu JF, Chen PC, Chang TM, et al. Monocyte chemoattractant protein-1 promotes cancer cell migration via c-Raf/MAPK/AP-1 pathway and MMP-9 production in osteosarcoma[J]. J Exp Clin Cancer Res, 2020, 39:254-261.
[15] 王雨,许达峰,周开伦,等.TLR4和肠道菌群在原发性肝癌进展中动态变化及与预后的关系[J].肝脏, 2020, 25: 273-276.