Abstract: Abstract：Objective This study was to investigate the effect of PARG-shRNA on liver metastasis of colorectal carcinoma CT26 cell line in mice. Methods Mice were divided at random into three groups. Animal models for Liver metastases of colorectal cancer were established by intrasplenic inoculation of colorectal carcinoma cell in BALB/c mice.CT26 cells transfected with empty vector and CT26 cells transfected with PARG-shRNA were inoculated to spleen caspsule.CT26 cells transfected with empty vector and untransfected CT26 cells served as control. The change of spleen and liver metastases carcinoma nodules were observed and counted. The expressions of PARG, PARP, NF-κB, integrin-β1,MMP-2,MMP-9 in spleen transplant tumor were measured by Western blot analysis. Results The size of spleen transplant tumor and liver metastatic nodules in transfected group were smaller than that in the control groups (p<0.05). The expression of PARG(0.0105±0.0028）、PARP（0.1786±0.024）、NF-κB（0.1678±0.0359）、integrin-β1、MMP-2、MMP-9 in transfected group was weaker than that in the control groups (p<0.05). Conclusion The growth of spleen transplant tumor and liver metastases can be inhibited by PARG gene silencing in CT26 cells. It is probably through inhibiting PARP, NF-κB and NF-κB-dependent gene downstream.

Key words: PARG-shRNA, colon carcinoma, liver metastases