Table of Content

05 July 2012, Volume 32 Issue 7

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Bone marrow mesenchymal stem cells transplantation promotes regenerati-

2012, 32(7):  727-733. 

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Abstract Objective To investigate growth and differentiation of adenovirus-green fluorescent protein labelled bone marrow mesenchymal stem cells (BM-MSCs) in uterus incision, and discuss its potential regeneration repair effect on uterine incision in rat. Methods BM-MSCs were separated by density gradient centrifugation and cultured until to the fourth generation, then were transfected and labelled with adenovirus-green fluorescent protein. Fourty female SD rats were divided equally into control and experimental group, followed with the establishment of uterine wound model. After two weeks, adenovirus-green labelled BM-MSCs were transplanted directly into uterus at previous incision, with the control group only received normal saline injection. Then, one month later, CTGF、CD34 and Masson was used to detect the outcome of the transplanted BM-MSCs.Results The transplanted BM-MSCs can growth well and showed some extent of differentiation toward smooth muscle cells. Significant difference existed for CTGF and Masson between experimental and control samples (both P<0.01).Conclusion BM-MSCs transplantation is a promising technique and can be used to promote regeneration repair of uterine incision.

Genistein Postconditioning protects against I/R injury through eNOS activation in hippocampal CA1 region of rats

2012, 32(7):  734-738. 

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Objective To investigate the neuroprotective role of genistein postconditioning (GPC) against cerebral ischemic injury and its effects on phosphorylation (activation) and protein expression of eNOS in hippocampal CA1 region of rats. Methods Cerebral ischemia was induced by four-vessel occlusion in rats and protein level of p-eNOS and eNOS were detected by Western Blotting. Additionally, NeuN staining was used to detect the survival neurons and apoptotic neurons of hippocampal CA1 region was observed using TUNEL analysis by Laser Scanning Confocal Microscope. Results In I/R groups, the number of NeuN-positive cell in hippocampal CA1 region was significant reduce accompanied with the increase of apoptotic neurons compared with sham groups. By contrast, treatment with Genistein after ischemia markedly protected the pyramidal neurons, as evidenced by the increase of NeuN-positive cell and the decrease of apoptotic neurons. Furthermore, L-NAME, an inhibitor of NOS abolished the neuroprotective role induced by genistein postconditioning. Additionally, immunoblot analysis showed that eNOS protein expression had no significant change in different times, while the level of p-eNOS significantly increased with two peaks occurring at 30min and 3d of reperfusion compared with I/R groups. L-NAME significantly suppressed enhance of p-eNOS induced by GPC at 3d of reperfusion. Conclusions GPC significantly prevents neuronal injury from global cerebral ischemia in the hippocampal CA1 region of rats. The possible mechanism is involved in the increase of p-eNOS expression.

Postnatal development characteristics of NG2 cells in spinal cords of rats

2012, 32(7):  739-743. 

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Objective To observe the postnatal development characteristics of NG2 cells in spinal cord of rats. Method Immunohistochemistry(IHC) was applied to examine the morphologic characteristics of NG2 cells. Western blot was used to observed the expression of NG2 in spinal cord during the different postnatal developmental stages. Result IHC showed NG2 cells persistently existed during different postnatal developmental stages in spinal cord. There were a great number of NG2 cells with few processes and round or oval cell bodies at P1d. At P7d, a few NG2 cells can be detected with short processes in spinal cord. But at P21d,few NG2 cells were examined in the spinal cord. At P60d,NG2 cells with irregular cell bodies and 1~3 short processes were revealed. Compared with the cerebral cortex in the corresponding stages,NG2 cells in spinal cord possessed bigger soma, shorter and thin processes.Western blot showed that NG2 expression was highest at P1d ,declined at P7d and reduced significantly lowest at P21d,but increased atP60d.Conclusion The NG2 cells persistently exist in spinal cord and are different in cell number and morphology at the different postnatal development stages. It may start myelination from P7d to P21d.NG2 cells in spinal cord may be at an earlier stage in oligodendrocyte lineage.

The effects and mechanisms of SD118-2 on HepG2 cells apoptosis

DAI yanyan

2012, 32(7):  744-750. 

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Objective: To study the effects of SD118-2 on human HepG2 cells proliferation 、cell cycle and cell apoptosis. Methods: The proliferation of cells was detected by MTT assay. The modality of cells was detected by the fluorescence microscope after DAPI staining. The cell apoptosis 、cell cycle and mitochondrial membrane potential were detected by the flow cytometer. The apoptosis associated with Bcl-2、Bax、PARP、C-PARP was detected by Western blots. Results :SD118-2 inhibited the proliferation of human HepG2 cells、decreased the mitochondrial membrane potential、induced cell apoptosis 、blocked the cells in G2 stage and down-regulated expression of Bcl-2、up-regulated expression of Bax、C-PARP at a time-dependent manner. At the same time, the effects of SD118-2 on normal human liver were very little. Conclusion : SD118-2 can inhibit the cell proliferation 、induce cell apoptosis、block the cell in G2 Stage of HepG2 .

Tmed2 accelerates murine MC3T3-E1 pre-osteoblast proliferation in vitro

2012, 32(7):  751-755. 

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Abstract: Objective Study the role of Tmed2 in murine pre-osteoblast proliferation. Methods 1. Over-express and knock down Tmed2, and the proliferation rate of MC3T3-E1 cell was then detected. 2. MC3T3-E1 cells were treated with β-Estradiol, cell number was then counted and the mRNA level of Tmed2 was analyzed. Quantitative real-time PCR was used to measure the mRNA level, MTS assay was used to assess the cell proliferation rate, flow cytometry was used to figure out the cell cycle distribution, and the protein level was evaluated by Western blot. Results Over-expression of Tmed2 accelerated MC3T3-E1 cell proliferation, and the proportion of cells in S phase markedly increased compared. The expression of Cyclin A also increased. On the contrary, Knockdown of Tmed2 decelerated MC3T3-E1 cell proliferation. In β-Estradiol promoted MC3T3-E1 proliferation, the mRNA level of Tmed2 remarkably increased. Conclusion Tmed2 accelerates murine pre-osteoblast MC3T3-E1 proliferation through up-regulating Cyclin A expression and therefore increasing the proportion of cells in S phase. In addition, the expression of Tmed2 is regulated by β-Estradiol, indicating that Tmed2 is possibly involved in the process of MC3T3-E1 proliferation promoted by β-Estradiol.

Expressional change of pancreatic derived factor (PANDER) in the pancreas of diabetic mice

2012, 32(7):  756-760. 

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Objective To analyze PANDER expression in pancreatic islets of type 1 and type 2 diabetic mice, and probe the correlationship of PANDER expression and insulin resistance in type 2 diabetic mice. Methods PANDER mRNA and protein levels were analyzed in the pancreas of STZ-induced type 1 diabetic mice, Akita diabetic mice, type 2 diabetic db/db mice. Result In the pancreas of STZ-induced and Akita diabetic mice, PANDER mRNA (STZ：1.00±0.17 vs. 0.25±0.06，P<0.01；Akita：1.00±0.19 vs.0.17±0.04，P<0.001) and protein (STZ：1.00±0.18 vs. 0.16±0.11) levels were significantly decreased when compared with non-diabetic control mice. In contrast, both PANDER mRNA (1.00±0.05 vs. 2.13±0.46，P<0.05) and protein (1.00±0.28 vs. 3.58±0.36，P<0.01) levels were increased in the pancreas of db/db mice as compared with db/m mice. Oral treatment of db/db mice for 1 month with rosiglitazone reduced pancreatic PANDER mRNA(1.00±0.13 vs. 0.28±0.06，P<0.01) and protein expression in pancreatic islets of db/db mice, with improved insulin resistance, hyperinsulinemia and hyperglycemia. Conclusions PANDER expression was reduced in the pancreas of type 1 diabetic mice, whereas its expression was increased in the pancreas of type 2 diabetic mice.

Txndc5 mediates estrogen accelerated proliferation of murine pre-osteoblast MC3T3-E1

2012, 32(7):  761-766. 

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Objective To investigate the role of Txndc5 in murine pre-osteoblast proliferation. Methods Murine pre-osteoblast cell line MC3T3-E1 was treated with estrogen. Txndc5 was either over-expressed by plasmid vector or knocked down by siRNA in MC3T3-E1 cell. The protein level of Txndc5 and Cyclins was evaluated by Western blot. The mRNA level of Cyclin A was measured by quantitative real-time PCR. Cell proliferation rate was determined by MTS assay and cell counting. Cell cycle distribution was revealed by flow cytometry analysis. Results The protein level of Txndc5 was up-regulated in estrogen-induced MC3T3-E1 proliferation. Knockdown of Txndc5 blocked estrogen-induced cell proliferation. Over-expression of Txndc5 accelerated MC3T3-E1 cell proliferation, increased the proportion of cells in S phase. Eighteen hours after the synchronized cells entered cell cycle, the expression of Cyclin A was up-regulated, and the percentage of cells in S phase was 18.69%±4.08% in cells over-expressing Txndc5, which was only 8.15%±3.68% in control cells. On the other hand, knockdown of Txndc5 inhibited the growth of MC3T3-E1 cells, decreased the proportion of cells in S phase, and down-regulated the expression of Cyclin A. Knockdown of Cyclin A attenuated the cell proliferation-enhancing effect of Txndc5. Conclusion Txndc5 mediates estrogen-accelerated pre-osteoblast proliferation through up-regulation of Cyclin A.

Profile of microRNA expression in Tumor Associated Macrophage and bioinformatics analysis

2012, 32(7):  767-772. 

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Objective To investigate the profile of microRNA expression in tumor associated macrophage (TAM). Methods An xenograft mouse model was established by using mouse breast cancer cell line 4T1. TAM were isolated from the tumor tissue. The microRNA expression profile was detected by using a microRNA chip assay. The result of chip assay was validated by real-time PCR and analyzed by bioinformatics. The peritoneal macrophage was used as control. Results There were significant changes in 59 microRNAs’ expression in TAM compared with the negative control. Among these microRNAs, 23 microRNAs’ expression was up regulated and 36 microRNAs down regulated. These microRNAs participate in the regulation of various signaling pathways. Conclusion Profile of microRNA expression and bioinformatics analysis suggested microRNA plays an important role in regulation of TAM differentiation.

SLC30A3 promotes early erythroid differentiation in human erythroleukemia K-562 cell line

2012, 32(7):  773-777. 

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Objective To study the role of SLC30A3 in erythroid differentiation. Methods After SLC30A3 was over-expressed by plasmid vector in human erythroleukemia K-562 cell line, markers of erythroid differentiation including CD235, ε-、γ- and β-globin, as well as cell proliferation rate were measured. Flow cytometry was used to assess the expression of CD235. Quantitative real-time PCR was used to measure the mRNA level of globins. The protein level of transcription factor GATA-2 was evaluated by Western blot. MTS assay was used to check the cell proliferation rate. Results Over-expression of SLC30A3 up-regulated the expression level of erythroid differentiation specific marker CD235. The percentage of CD235(+) cells increased to 95.7%±0.14% compared to that of the negative control which was 34.25%±16.89%. The expression of ε-、γ- and β-globin were also remarkably up-regulated. The expression of erythroid differentiation-related transcription factor GATA-2 was down-regulated. And the cell proliferation rate was accelerated in response to SLC30A3 over-expression. Conclusion SLC30A3 promotes early erythroid differentiation in human erythroleukemia K-562 cell line.

Lysis of autologous HIV-infected and uninfected CD4 T cells by γδ T cells from AIDS patients

2012, 32(7):  778-782. 

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Objective: Establish a model of autologous Human Immunodeficiency Virus (HIV)-infected CD4 T cell, to investigate the cytotoxicity of γδ T cells of acquired immunodeficiency syndrome (AIDS) patients to autologous HIV-infected and uninfected CD4 T cells. Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from healthy donors and AIDS patients. CD4 T cells with high purity were obtained through negative isolation using magnetic activated cell sorting (MACS). To establish the autologous HIV-infected CD4 T cell model, CD4 T cells from AIDS patients were stimulated with phytohemagglutinin (PHA) and co-cultured with interleukin-2 (IL-2). Percentages of HIV-p24+ CD4 T cells were detected by flow cytometry. Cytotoxicity of γδ T cells was determined by Lactate dehydrogenase (LDH) assay. Results: The percentages of HIV-p24+ CD4 T cells reached the peak at 10 days. γδ T cells from AIDS patients can kill both the autologous HIV-infected and uninfected CD4 T cells (the cytolysis rate were 71.1 ± 9.7 and 24.2 ± 18.2，respectively). However, γδ T cells from healthy donors do not kill the autologous CD4 T cells (the cytolysis rate was 4.3 ± 10.1). Conclusions: γδ T cells of AIDS patients can kill the autologous HIV-infected CD4 T cells, which provide a new sight for the clinical therapy of AIDS based on γδ T cells adoptive immunotherapy.

Overexpression of miR-195 promotes apoptosis in glioma T98G cells

Bin YIN Xiao-zhong PENG

2012, 32(7):  783-787. 

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Abstract：Objective To investigate the influence of overexpression of miR-195 on cell proliferation in glioma T98G cells and explore the function of miR-195 in glioma. Method T98G cells containing lower expression level of miR-195 were transfected with synthesized miR-195 mimics , then MTT assay, flow cytometry and TUNEL assay were performed to analysis the alterations of cellular phenotype，western blot was performed to examine the expression level of BCL-2. Result Mature mir-195 increased significantly after miR-195 mimics transfection (P<0.05). Overexpression of miR-195 induced significant cell proliferation suppression. Flow cytometry results showed that up-regulated miR-195 produced little influence on cell cycle, while TUNEL assay indicated that miR-195 promoted apoptosis in T98G cells. Western blot result showed that BCL-2 was reduced after by miR-195 mimics. Conclusion Overexpression of miR-195 significantly promoted cell apoptosis in T98G cells, suggesting that miR-195 may function in glioma treatment.

Effects of Ca2+ and NO on increased normal adult rat’s mesentery mesenteric microvessel permeability induced by H2O2

2012, 32(7):  788-792. 

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Objective: To study the effect of endothelial [Ca2+]i and NO production on H2O2 induced increased mesenteric microvessel permeability in normal adult rats. Methods: Microvessel permeability was assessed by measuring hydraulic conductivity (Lp). H2O2-induced changes in endothelial [Ca2+]i and NO production were measured in Fura-2 AM (Ca2+ fluorescent indicator) or DAF-2 DA (NO fluorescent indicator) loaded microvessels in vivo via fluorescence microscopy. Results: H2O2 can increase mesenteric microvessel permability (6.13±0.87 times control value, P<0.01）and increase endothelial [Ca2+]i (714.58±144.70 nmol/L, P<0.01）and NO production (1034.3%±44.3% of control ?uorescence intensity, P<0.01)in normal adult rats. Calcium channel blocker, Lanthanum Chloride (LaCl3), inhibited the increase in microvessel permeability (P<0.01) and endothelial [Ca2+]i (P<0.01) induced by H2O2. NOS inhibitor, AP-Cav-1, can inhibit increased microvessel permeability induced by H2O2 (P<0.01), but have no effect on increased endothelial [Ca2+]i. Conclusion: Increased microvessel endothelial [Ca2+]i and NO production is involved in H2O2 induced increases in microvessel permeability.

MDA-7/IL-24 inhibits the growth and induces the apoptosis of human breast cancer MDA-MB-231 cell line

2012, 32(7):  793-797. 

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Abstract:Objective To explore the effect of IL-24 gene on proliferation and apoptosis of human breast cancer cell line MDA-MB-231. Methods The recombinant shuttle vector pDC316-hIL-24-EGFP was transiently transfected into human breast cancer cell line MDA-MB-231 with lipofectamine. Both IL-24 mRNA and protein were detected by RT-PCR and Western blot respectively.The inhibition of cell proliferation was evaluated by MTT assay. Changes in cell cycle were detected by flow cytometry(FCM) .The cell apoptosis was studied by Hoechst 33258 staining. Results　IL-24 was proved successfully transcribed and expressed in MDA-MB-231 cells. IL-24 enhanced protein Caspase-3 expression level of MDA-MB-231cells. The IL-24 expression inhibited MDA-MB-231 cells proliferation. In MDA-MB-231cells of IL-24 over-expressed the DNA synthesis was inhibited and arrested in G2/ M phrase according to FCM.Increased apoptosis of MDA-MB-231 cells were observed by Hoechst33258 staining.Conclusion IL-24 gene overexpressed was able to effectively inhibit the proliferation and promote the apoptosis of breast cancer cells,which may be due to enhancing of protein Caspase-3 leve.

Isolation of mesenchymal stem-like cells from human esophageal carcinoma and identification of their biological characteristics

2012, 32(7):  798-803. 

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Abstract Objective To isolate mesenchymal stem-like cells from human esophageal carcinoma and to identify their biological characteristics. Methods hEC-MSCs were isolated by collagenase digestion.Gene expression were detected by RT-PCR; surface antigen markers and cell cycle were determined by flow cytometry. Growth curve was portrayed and chromosme karyotype was assayed.Multilineage differentiation capacity of the cells was tested by inducing differentiation toward osteoblasts and cardiomyocytes. Results hEC-MSCs isolated by collagenase tended to be long and stringy in shape and multiplied rapidly.The cells expressed related gene Oct-4,Nanog,vimentin and N-cadherin,but not E-cadherin；FCM results showed that The cells expressed CD13, CD29,CD44 and CD105,but did not express CD33,CD45, CD133,CD14,CD34 and HLA-DR.Cell cycle analysis revealed that the majority of cells stood in G0/G1 phase while a small population of cells were in S and G2/M phase.The cells contain 46 chromosomes with normal Karyotype and they have the potentials differentiated into osteoblasts and cardiomyocytes.Conclusion hEC-MSCs can be isolated efficiently by collagenase digestion and they have important significance for further studying the microenvironment of esophageal carcinoma.

The Effect Of Bone Marrow Mesenchymal Stem Cells Derived HGF To Modulate Apoptosis And The Expression Of DR5 And Caspase-8 In Rat Hepatic Stellate cells

2012, 32(7):  804-808. 

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Objective: To observe rat bone marrow mesenchymal stem cells (BMSCs) derived hepatocyte growth factor (HGF) effects on primary hepatic stellate cells (HSCs) death receptor-5 (DR5), Caspase-8. To study part of the mechanism of BMSCs derived HGF to promote apoptosis of HSCs. Methods: BMSCs were isolated from SD rats, cultured and purified in vitro. Primary HSCs were cultured in plastic plates. BMSCs were cultured in the Transwell insert and the HSCs were cultured in the plastic plates(6-wells) establish the upper and lower double-cell co-culture system. Cultured 24h，48h，72h；divided into four groups: Group A: HSCs blank group: HSCs cultured alone; ② Co-cultured group: BMSCs co-cultured with HSCs; ③ TNF-a Pretreatment group: BMSCs stimulated by TNF-a six hours later discard the supernatants and then co-cultured with HSCs with fresh medium ; ④ HGF-ShRNA interference group. Cell supernatants were harvested to determine the concentration of HGF and TRAIL by ELISA. Apoptosis of HSCs was determined by Annexin-V-FITC/PI; the expression of DR5 mRNA、Caspase-8 mRNA and two kinds of protein in HSCs was determined by FQ-QPCR and Western blot respectively. Results: 1. Concentration of HGF and TRAIL in each group Supernatants were determined by Elisa: Concentration of HGF in TNF-a pretreatment group obviously higher than that in BMSCs group and HSCs blank control group (P<0.01); Concentration of TRAIL in the BMSCs group was higher than that in HSCs blank control group and TNF-a pretreament group; 2. Flow cytometric Annexin-V-FITC/PI test shew: apoptosis rate of HSCs in TNF-a pretreatment group was obviously higher than that of the other three groups and had time-dependent (P<0.01); Apoptosis rate of HGF-ShRNA interference group was lower than that of BMSCs+HSCs cocluture group and higher than HSCs blank control group (P<0.05). 3. The expressions of DR5、Caspase-8 protein and mRNA in HSCs was lower than BMSCs+HSCs cocluture group (P<0.01); Those of TNF-a pretreatment group was significantly higher than HSCs blank control group and HGF-ShRNA interference group (P<0.01). Conclusions: BMSCs co-cultured with HSCs to promote apoptosis of HSCs, which may be associated with BMSCs derived HGF increasing the expression of HSCs DR5 and Caspase-8.

Morphine preconditioning attenuated MCAO-induced brain ischemic injuries of mice

LIU Ya Bing-Xi Zhang Jun-fa LI

2012, 32(7):  809-813. 

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Abstract: Objective To investigate the effects of morphine preconditioning (MP) on middle cerebral artery occlusion (MCAO)-induced brain injuries and its possible mechanisms. Methods MCAO mouse models were established. The brain infarction sizes and edema ratio were determined by 2,3,5-Triphenyltetrazolium chloride (TTC), the neurological deficit score was also evaluated. Samples were collected from left cerebral cortex of Sham group, ischemic core (I), penumbra (P) and contralateral cortex (C) from both MCAO and 10mg/kg MP groups. Western blot analysis was used to determine Hsp70 protein expression levels. Western blot analysis was used to determine Hsp70 protein expression levels. Results The brain infarct size of MCAO group was 31.69%±4.19%, the edema rate was 8.98%±1.79%. MP reduced infarction size and edema ratio significantly compared to MCAO group (P<0.05), there were 23.34%±4.85% and 4.60%±0.86% respectively. MP could also improve MCAO-induced neurological deficits score of mice (P<0.05). Compared with that of Sham group, Hsp70 expression in mice under MCAO increased significantly (P<0.05), there was much higher expression of Hsp70 in the penumbra of MP group but not in the ischemic core and contraletaral compared with that of MCAO group. Conclusion Morphine preconditioning can attenuate MCAO-induced focal brain ischemic injuries in mice, and the high level of Hsp70 in penumbra may be involved in the neuroprotection.

Expression of nervous system related gene in retinoic acid –induced neural tube defects of mice

2012, 32(7):  814-818. 

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Abstract: Objective To study the expression of four nervous system related genes: ASH2L, HDAC4, NSPC1and DOK5 in the all trans retinoic acid -induced mouse neural tube defect model. Methods: Divide eight E8.5 C57/BL6 pregnant mice randomly into two groups: control group and experimental group . Control group, intraperitoneal injection of olive oil, the experimental group by intraperitoneal injection of all-trans retinoic acid; E13.5 take embryos. Real-time PCR were detected in mouse brain and spinal cord of ASH2L, HDAC4, NSPC1 and DOK5 mRNA expression; Western Blotting detected two groups of mice brain and spinal cord ASH2L, HDAC4, NSPC1 and DOK5 of protein expression.Results All-trans retinoic acid can induce a typical neural tube defects in mice. Compared with the control group, the expression of mRNA declined in fetal mice brain and spinal cord which is treated with all trans RA (P <0.05); Meanwhile, the protein levels of these genes was also significantly decreased (P <0.05). Conclusion ASH2L, HDAC4, NSPC1 and DOK5 may be the may underlying genes of inhibiting all trans RA-induced neural tube defects.

Clinical analysis of gout outpatient database from Peking Union Medical College Hospital

2012, 32(7):  819-822. 

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Objective Analyse the clinical characteristic of gout outpatients from Peking Union Medical College Hospital. Methods Questionnaire survey and clinical analysis was conducted to collect and analyse 358 gout outpatients’ clinical data and feature. Results The proportions of males and females were 97.8% and 2.2% respectively. Among the 358 patients with the average duration of (100.1±76.7) months and mean serum uric acid level of (601.49±11.16) umol/L, multi-joints affected patients accounted for 79%. Tophus was found in 77(21.5%) paitents, and tophus affected hands, metatarsophalangeal joints and auricle. 71.5% had coexistence of basic diseases. Conclusion Young and middle-aged patients are in the majority. Most patients have multi-joins affected with a high rate of underlying disorder coexisting. Clinical presentation is diversified and complicated.

Tran-2,4-dimethoxystibene (S3) promoted the learning and memory in model rats induced by hypercholesterolemic with i.c.v. injection of Aβ25-35

2012, 32(7):  823-827. 

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Abstract Objective To investigate the effect of S3 on learning and memory in model rats induced by hypercholesterolemic with i.c.v. injection of Aβ25-35. Method 70 female Wistar rats were divided into 7 groups. Except the normal group, other 60 rats were fed with hypercholemic chow for six weeks, and then received an intracerebroventricular injection for once. The E2 and S3-treatment groups’ rats were treated with E2 or S3 for another 7 days. Behavioral changes were evaluated by Morris water maze and step-down test. The activities of choline acetyl transferase (ChAT) and acetylcholine esterase (Ach E) were analyzed by spectrophotometric method, and the content of acetylcholine was by ELISA. Result The data shown that S3 dose-dependently decreased serum total and LDL-C levels (P<0.01) in model rats with hypercholesterolemic plus i.v.c. injection of Aβ25-35. The highest dose of S3 shortened the escape latency significantly. The step-down latency of S3 treated groups was restored to near that of the control group and the number of errors was reduced markedly. Meanwhile, S3 reversed the decreased activity of ChAT as well as the increased activity of Ach E in hippocampus. Conclusion These findings suggest that S3 improved the model rats learning and memory by decreased the serum cholesterol, increased the concentration of Ach in hippocampus through changes of ChAT and Ach E activities.

Antagonistically inhibitory effect of Fluorouracil combined with Anthocyanin to the proliferation of gastric cancer cell SGC-7901 in vitro

2012, 32(7):  828-830. 

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Puerarin and other reducing lead poisoning or ovariectomized in rats osteoporosis

2012, 32(7):  831-832. 

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[Abstract] Objective: Through observation of puerarin on osteoporosis in ovariectomized rats and rats with lead poisoning Haierfu on the treatment of osteoporosis, as in Traditional Chinese treatment of osteoporosis and postmenopausal osteoporosis in rats with lead poisoning and provide experimental Basis.. Methods: 5 months old female rats 100, divided into five experimental groups (n = 20): (1) control group (Nomal); (2) to the ovariectomy group (OVX); (3) lead poisoning model group (PLD); (4) OVX + puerarin group (P + OVX); (5) lead poisoning Haierfu + group (H + PLD), the experimental group at 12 weeks observed from rat femur bone tissue biopsy, blood Measuring serum calcium, phosphorus and alkaline phosphatase, the data were statistically analyzed.. Results: OVX group and the PLD group showed a light microscope, the pathological changes of osteoporosis, calcium, phosphorus was significantly lower than Nomal group (P <0.01), while the ALP was significantly higher than Nomal group (P <0.01). P + OVX group and the H + PLD group compared with the Nomal calcium, phosphorus, and alkaline phosphatase were no significant differences (P> 0.05), 2 treatment groups had no significant pathological changes of osteoporosis. Conclusion: Puerarin on ovariectomized rats osteoporosis and Haierfu on osteoporosis in rats of lead poisoning have a good therapeutic effect; lead poisoning can cause osteoporosisAnd reduced hemoglobin

Development of translational medicine in the tumor thermotherapy and tumor immunology

2012, 32(7):  833-836. 

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Thermotherapy becomes another important cancer treatment apart from the surgery, radiotherapy, chemotherapy and biological therapy. Heat shock proteins and dendritic cells which influenced by hyperthermia, correlate with tumor immunology, and was studied from bench to bedside. According to these researches, the development of translational medicine in combination of thermotherapy and tumor immunology has been in concern, and may change the status in quo of the treatment of cancer.

The advancement on the mitochondrial-associated apoptosis protein

2012, 32(7):  837-840. 

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Apoptosis, a form of intrinsic cellular suicide program, is closed related with the occurrence and development of cancer or neurodegenerative diseases. The current study confirmed that mitochondria is the cener place of apoptosis proceeding, and mitochondrial pathway is an important pathway of apoptosis. Some mitochondrial-associated proteins, such as: Bcl-2 family proteins, apoptosis-inducing factor, Cytochrome C, etc, play an important role in the process of apoptosis respectively. Meanwhile, these proteins can cross-talk and regulate apoptosis coordinately. In this review, the focus will be put on the main mitochondrial apoptosis-related proteins and their latest research.

Activating protein-2 transcription factors and its roles in breast cancer

2012, 32(7):  841-843. 

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Abstract：AP-2，a nuclear transcription factor family ，are involved in the regulation of cell proliferation, differentiation, apoptosis and carcinogenesis.At least 3 of the five AP-2 family members identified to date, AP-2a, AP-2βand AP-2γ, are related with the development of the breast cancer; transactivation fuction of AP-2 is regulated by tumor suppressor gene WWOX and P53、HER2 oncogene、trascriptional coactivators and AP-2 protein modifications.

Advances in the Organ Protection of Dexmedetomidine

2012, 32(7):  844-846. 

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Abstract Dexmedetomidine is a novel and highly selectiveα2-adrenoceptor agonist. Dexmedetomidine offers beneficial pharmacological properties, providing dose-dependent sedation, analgesia, sympatholysis and without relevant respiratory depression. Resent studies of dexmedetomidine has proved that it can maintain hemodynamics during the perioperative period,decrease sympathetic tone,reduce the consumption of anaesthetic,prevent postanesthetic shivering and provide organ protection. This review aims to present the organ protection of dexmedetomidine.

The Application of Virtual Microscopy Technology In Pathology

2012, 32(7):  847-849. 

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The application of virtual microscopy technology to morphology teaching can significantly improve teaching and learning efficiency, and the combination of virtual microscopy technology with digital microscope mutual system complement each other; in clinic virtual microscopy technology is mainly used in remote pathology consultation, slides archiving, and is a important assistant technology. With the development and improvement of virtual microscopy technology, virtual microscopy technology will be applied more popularly, and promotes continuous development of the pathology and pathological diagnosis.

Current Situations and Improvement Strategies of Medical Morphology Lab Science Education

Hong-Cheng ZHU

2012, 32(7):  850-852. 

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It is great exploration to develop the course of Medical Morphology Lab Science, though some problems occur. Measures should be taken to combine the theory and practice, to diversify teaching methods, to innovate experimental content and to create efficient and reasonable assessment methods. Benefits of this course should be made full use of to train qualified medical personnel.