Abstract: Objective: To study the effects of SD118-2 on human HepG2 cells proliferation 、cell cycle and cell apoptosis. Methods: The proliferation of cells was detected by MTT assay. The modality of cells was detected by the fluorescence microscope after DAPI staining. The cell apoptosis 、cell cycle and mitochondrial membrane potential were detected by the flow cytometer. The apoptosis associated with Bcl-2、Bax、PARP、C-PARP was detected by Western blots. Results :SD118-2 inhibited the proliferation of human HepG2 cells、decreased the mitochondrial membrane potential、induced cell apoptosis 、blocked the cells in G2 stage and down-regulated expression of Bcl-2、up-regulated expression of Bax、C-PARP at a time-dependent manner. At the same time, the effects of SD118-2 on normal human liver were very little. Conclusion : SD118-2 can inhibit the cell proliferation 、induce cell apoptosis、block the cell in G2 Stage of HepG2 .

Key words: key words： SD118-2 , HepG2 cells , apoptosis , cycle , the mitochondrial membrane potential , Bcl-2 , Bax , PARP , C-PARP .