Table of Content

05 November 2011, Volume 31 Issue 11

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Conjoint Analysis the Gene Polymorphism of ACE and AGT in hypertension disease complicated with cerebral infraction

2011, 31(11):  1189-1193. 

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Objective To explore the association between the ACE, AGT gene polymorphism and hypertension disease complicated with cerebral infraction．Methods The ACE gene I/D polymorphism and the AGT gene M235T polymorphism were detected in a study of 664 cerebral infraction (CI) cases, 678simple essential hypertension (EH) cases and 716 control cases (C). The association of gene polymorphism and hypertension disease complicated with cerebral infraction is evaluated. Results The genotype frequencies of ACE-DD and AGT-TT in the CI group (0.309 and 0.643) were significantly higher than those in the control group (0.203 and 0.543) and EH group (0.217 and 0.569), respectively. The OR value of combining ACE-DD and AGT-TT of CI to the control group (2.547，95%CI : 1.919~3.382) is significantly higher than the OR value of single ACE-DD genotype (1.759，95%CI : 1.376~2.248) or single AGT-TT genotype（1.515, 95%CI : 1.220~1.880). Conclusions These results suggest that ACE-DD or AGT-TT variant is not associated with Chinese hypertensive patients, but is significantly associated with Chinese hypertensive patients complicated with cerebral infraction. ACE-DD and AGT-TT may have a synergistic effect on risk of hypertension disease complicated with cerebral infraction.

Mobiliztion and recruitment of very small embryonic-like stem cells in Focal Cerebral Ischemia mouse

2011, 31(11):  1194-1199. 

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Abstract:Objective To investigate the mobilization of bone marrow very small embryonic-like stem cells(VSEL-SCs) into peripheral blood （PB）and its ’recruitment to the infarcted brain tissues in focal cerebral ischemia mouse.Methods: MCAO model was induced by using the filament occlusion method.G-CSF, the CXCR4 specificity antagonist AMD3100 and combilation used were seperatedly injected to MACO model.Neurological scale were evaluated . The number of VSEL-SCs mobilized into PB was check by fluorescence-activated cell sorting analysis( FACS ) . The level of SDF-1 in plasma and cerebral tissue were checked by ELISA assay . Expression of SDF-1 in the regions of ischemia were checkd by immuncytochemistry. Results: the neurological scale of G-CSF treated group significantly diseaseed compared with the control group (P<0.05), AMD3100 showed no significantly effect.All three operated groups mobilized more VSEL-SCs into PB compared with the control group,the effective sequence is AMD300 combine with G-CSF, AMD300, G-CSF. The plasma SDF-1 density significantly incresed in G-CSF group at 72h and 108h (P<0.05)while AMD300 and A+G group show stronger effect at all three time point(24h,72h,108h). Positive correlation can be seen between the number of VSEL-SCs mobilized into PB and the SDF-1 plasma concentration .The SDF-1 level of cerebral tissue were significantly incresed in G-CSF group and the expression of SDF-1 in ischemia region were much stronger in G-CSF group and A+G group.Conclusion: G-CSF and AMD3100 mobilized VSEL-SCs in adult MACO mouse bone marrow into periphril blood. The mechanism of beneficial effects of G-CSF possibly related to increasement of SDF-1 expression in the infarcted tissues and recruitment of more VSEL-SCs through CXCR4/SDF-1 axis . Key Words:very small embryonic-like stem cells ;Bone marrow stem cells; mobilization; ischemic stroke

Effect of atorvastatin on myocardial injury in rats following co-stress of myocardial ischemia and cold

2011, 31(11):  1200-1204. 

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Abstract: Objective To analyze the effect of co-stress of myocardial ischemia(MI) and cold on myocardium in rats,and investigate the role and relative mechanism of atorvastatin intervention in such co-stress.Methods Co-stress model was induced by ligation of left coronary artery combined with cold stress of 4℃,8h/d for 4 consecutive days.Rats were gavaged with atorvastatin 20mg.kg-1.d-1 for 3 days before MI was induced and thereafter for 4 days.Cardiac function was assessed by echocardiography;myocardial infarct size was determined by TTC staining;p- PI3K,p-GSK3β,Bim and Caspase-3 expression in myocardium was determined by western blotting.Results It was demonstrated that co-exposure of myocardial ischemia and cold stress could significantly deteriorate the cardiac function and increase the infarct size (P<0.01),and this was attenuated by atorvastatin use with increased expression of p-PI3K,p-GSK3β and Bim, Caspase3 downregulating(P<0.01).Conclusion Co-exposure to myocardial ischemia and cold stress aggravate the cardiac injury, this influence can partially attenuated by atrovastatin use and the mechanism may be attributed to activation of PI3K/Akt/GSK3β and decreasing expression of Bim.

Migration of in vitro expanded γδ T cells toward tumor tissue

2011, 31(11):  1210-1216. 

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Abstract Objective To investigate the migration tendency of the expanded γδ T cells toward colorectal cancer cells. Methods Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation and stimulated by immobilized T cell receptor (TCR) gd specific antibody in vitro for two weeks. The expanded cells underwent immunofluorescence staining and flowcytometry analysis or flow sorting. The cytokines and chemokines expressed by gd T cell were assayed using the Bioplex 200 Suspension Array System. The chemotaxis assays were performed in the transwell chambers. Results Those in vitro-expanded γδ T cells primarily expressed chemokine receptors CCR5 and CXCR3. CCR5 and CXCR3 ligands were detected in four different colorectal cancer cell lines and in ten cases of colorectal cancer. Upon TCR engagement, expanded γδ T cells produced large amounts of Th1 and Th2 cytokines, which further increased γδ T cell accumulation. It is noteworthy that the interferon γ (IFN-γ) produced by the γδ T cells markedly increased the expression of CXCR3 ligands in colorectal cancer cells, which further boosted the migration of the expanded γδ T cells. Conculsion Our data may provide important indications for the use of adoptive γδ T cell-based immunotherapy for the treatment of colorectal cancer.

Constriction and identification of mice model with cardiac specific disruption of cardiac ankyrin repeat protein

2011, 31(11):  1217-1222. 

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Abstract: Objective To establish the mice model with cardiac disruption of cardiac ankyrin repeat protein (CARP) and to provide reliable tools for studying the function of CARP gene in heart related diseases. Methods Construct the conditional target plasmid of PL253-CARP-LoxP and transfect to 129 embryonic stem cells. Positive clones selected by antibiotics of G418 and GANC were determined by Southern blot to confirm conditional disruption of CARP gene, and then were injected to the blastula. Chimeras were got and crossed with α-MHC-Cre transgenic mice, which expressed recombinase specifically in heart, and then the mice with cardiac deficiency of CARP gene (CARP-KO) will get. Results Six chimeras were got, and CARP-KO mice were got after crossing with α-MHC-Cre mice. CARP gene was disrupted in the heart of CARP-KO mice by semi-quantitative PCR and western blot. These mice displayed normal in embryonic development, body growth, and heart development. However the expression of hypertrophic markers of β-MHC and ANF increased significantly in CARP-KO mice. Conclusion: The mice model with specific disruption of CARP gene was generated successfully. The cardiac disruption of CARP gene did not interfere the development and growth of mice significantly, either for the heart development.

Sirt1 enhances drug resistance of CML K562 cells

2011, 31(11):  1223-1228. 

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Objective to explore the function of Sirt1 in drug resistance of CML（chronic myelogenous leukemia）K562 cells. Methods K562 cells stably expressing dominate negative Sirt1 (H363Y) or Sirt1 shRNA were treated with H2O2 and etoposide. Cleaved caspase3, Bax and γ-H2A.X signals were detected by Western blotting; MCF-7 and 293A cells overexpressing wild type Sirt1 were treated with H2O2 and etoposide. γ-H2A.X was detected by Western blotting. Results Sirt1-H363Y and Sirt1 shRNA expression in K562 cells could enhance the cleavage of caspase3 and the expression of Bax, but inhibit the formation ofγ-H2A.X induced by H2O2 and etoposide. On the contrary, overexpression of wild type Sirt1 in MCF-7 and 293A cells promotes the formation ofγ-H2A.X. Conclusion Sirt1 could enhance the drug resistance and signal for DNA repair in K562 cells.

Relationship Between SOD2 Expression and Free Radical Level in Neurodegeneration Induced by Ischemia-reperfusion

2011, 31(11):  1229-1233. 

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Objective To investigate the changes of time process of SOD2 and free radicals in neurodegeneration induced by cerebral ischemia-reperfusion(I/R). Methods Cerebral I/R model was established through drawing out and reperfusing 40% of the whole blood volume in combination with clamping the carotid arteries for 20 min. Histological observation was performed to evaluate the neural damage; learning and memory ability was detected by Morris water maze; SOD2 activity and malonaldehyde(MDA) were determined by spectrophotometer; and expression of SOD2 was detected by western blot. Results Compared with sham group, the learning and memory ability of I/R group was significantly decreased, the hippocampal MDA level of I/R group was clearly increased in a time-dependent manner (P<0.05). SOD2 activity (14.69+2.49 U/mg.pro, 14.15+2.41 U/mg.pro, 15.07+2.67 U/mg.pro, 15.55+2.64 U/mg.pro）and protein expression (9.20+1.23, 5.40+1.03, 4.90+0.92, 5.60+0.59) of 5th d, 15th d, 30th d, and 60th d I/R group were lower than sham group (P<0.05). Histological test indicated there existed the loss and karyopyknosis of neurons in hippocampi of I/R group. Conclusion I/R-induced neurodegeneration may be related to the steady decreases of SOD2 expression and activity and increase of oxidative stress.

Implementation survey of comprehensive smoke-free policy in Jiamusi medical institutions

2011, 31(11):  1234-1237. 

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Abstract: Objective To study the implementation of smoke-free policy in Jiamusi medical institutions. Methods The multi-stage sampling method was used to choose 14 medical institutions in Jiamusi. The methods of personal interview, site observation and intercept survey were used to collect related information of tobacco control activities, the smoke-free regulations and secondhand smoke exposure in Jiamusi medical institutions. Results Although there is no municipal level smoke-free regulation in Jiamusi, the health institutions launched a series of tobacco control activities. Most leaders in medical institutions expressed supports for tobacco control publicly and developed a comprehensive smoke-free policy. In 204 specific observation points, the percentage of people smoking was 9.80%, the percentage of finding cigarette butts was 23.04%, the percentage of finding non-smoking signs in the institutions was 28.43%. According to the intercept survey, the percentage of smokers smoking indoor reached 28.30%, the percentage of seeing other people smoking indoor reached 15.23%, the percentage of knowing the comprehensive smoke-free policy was 63.79%. Conclusions Jiamusi medical institutions launched a series of tobacco control activities for the enforcement of smoke-free medical institutions by the end of 2011 and had active effects, but they did not meet the requirements of comprehensive smoke-free environment, we should advocate legislation in municipal level and mobilize people in the institution in the future.

Study of the relationship between PAI-1 promotor region 4G/5G gene polymorphism,ACE I/D gene polymorphism and cerebral stroke

2011, 31(11):  1238-1241. 

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【Abstract】 Objective To investigate the relationship between gene polymorphism of the plasminogen activator inhibitor-1 (PAI-1) promotor region 4G/5G gene, angiotensin I converting enzyme gene insertion/deletion (I/D) and cerebral stroke. Methods The genotype of 4G/5G allele polymorphism in the PAI-1 promotor region and I/D allele polymorphism in ACE were determined by polymerase chain reaction from leukocytes of 139 normal controls and 203 patients with cerebral stroke. Serum ACE activity was measured by colorimetry, plasma level of PA I-1 activity was determined by spectrophotometric assay. Results The plasma PAI-1 activity (0.769±0.163 AU/mL) and ACE activity in serum (43.42±14.36 U/L) in cerebral ischemia group (CI group) were significantly higher than those in control group (0.652±0.116 AU/mL, 31.28±8.64 U/L, p < 0.01, respectively). The frequency of PAI-1 4G/4G genotype and 4G alleles (43.14% and 62.24%), ACE D/D genotype and D alleles (49.02% and 67.16%) in CI group was significantly higher than those in control group (24.46% and 51.79%; 20.86% and 42.09%, p < 0.05, respectively). However, the difference of these data was not significant between cerebral hemorrhage (CH) and Control group. Furthermore, the effects of PAI-1 4G/4G genotype and ACE D/D genotype on cerebral infarction were synergetic. Conclusion 4G/4G PAI-1 and D/D genotype in ACE may be a susceptible factor to acute cerebral infarction, 4G and D allele homozygous genotype may be the major and synergetic risk factors of acute cerebral infarction.

Enriching the Fetal Nuclear Red Blood Cells by Intervening

2011, 31(11):  1242-1246. 

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Abstract：Objective To investigate the effectiveness of the Nucleated Red Blood Cells’ enrichment by intervening membrance ionic channels- K+/Cl- cotransporter and density gradient centrifugation in umbilical cord blood. Methods Setting up optical Intervening condition of maximal change of cell volume from RBCs on the umbilical cord blood, these rates of cell’s enrichment by different density medium centrifugation were determined by flow cytometry. Results Compared to traditional rich method, the average purity of NRBC enrichment in the umbilical cord blood could take from 8.6% to 69.9% and 55.9% by centrifugation of 1.065g/L medium after intervening and 1.099g/L medium,which means to respectively increase about .8 and 6.5 times enrichment of NRBC. Conclusions Both urea intervention and density centrifugation could effectively promote the rate of the NRBC’enrichment .

Effect of Curcumin Preconditioning on Expression of Myocardial Apoptosis-related Factor in Acute Myocardial Ischemia in rats

2011, 31(11):  1247-1250. 

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【Abstract】0bjective to investigate the effect of curcumin preconditioning on expression of myocardial apoptosis-related factor in acute myocardial ischemia (AMI) in rats. Methods Thirty SD male rats were randomly divided into acute myocardial ischemia injury rats group, sham-operation group and curcumin-treated AMI group. Detected myocardial apoptosis with the terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) technique of all rats．Then infered their apoptosis index．We detected myocardial apoptosis-related factor with RT-PCR technique of all rats and investigated their relations．Results Compared with acute myocardial ischemia injury rats group，their mRNA levels of bcl-2 in curcumin-treated AMI group increased markedly, the myocardial apoptosis index related positively with their mRNA levels of bcl-2; their mRNA levels of caspase-3 in curcumin-treated AMI group decreased signaficantly, the myocardial apoptosis index related negtively with their mRNA levels of caspase-3. Conclusions Curcumin have obviously protective effects on myocardial infarction in the myocar- dial ischemia rats．The mechanism possible related with regulation apoptosis-related factor.

Construction of the Recombinant Adenovirus Expressing the CVB3 VP1 protein and study on its immunological effects in mice

2011, 31(11):  1251-1255. 

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Objective To construct recombinant adenovirus Ad/VP1 and investigate the immune effect against coxsackievirus infection in mice. Methods The recombinant adenovirus Ad/VP1 was constructed and packaged. The target protein was verified by Western blot analysis. BALB/c mice were divided into three groups: Ad/VP1 group, Ad group and PBS group. The mice in each group were immunized by intramuscular injection. The titers of sera IgG and neutralizing antibody were detected by ELISA method and trace neutralization assay, respectively. The specific CTL cytotoxic activity was detected by CCK-8 assay. The mice in each group were challenged with lethal dose of coxsackievirus, the titers of the sera virus were titrated and the protection efficacy against coxsackievirus infection were observed. Results The recombinant adenovirus Ad/VP1 was successfully constructed and target protein was expressed. It’s observed that the titers of CVB3 VP1 specific antibody and neutralizing antibody was much higher than those of the other groups(P<0.01), CTL cytotoxicity activities and protection rate of the Ad/VP1 group were also much higher than the other groups(P<0.05), and the titer of sera virus was lower after CVB3 challenged(P<0.05). Conclusions Both the celluar and humoral immune responses and the protection rate in mice could been significantly enhanced by the Ad/VP1.

The role of Noggin protein in proliferation and invasion of breast cancer cell line MDA-MB-231

2011, 31(11):  1256-1260. 

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Abstract: Objective To investigate the effecti and mechanism of Noggin protein in proliferation and invasion of human breast cancer cell line MDA-MB-231. Methods MDA-MB-231 cells were infected with recombinant Noggin adenovirus. MTT, Wound-healing experiment and Matrigel invasion assays were performed to examine the change of proliferation, movement and invasion. The expression of MMP-1 and CXCR4 was evaluated by real-time PCR and Western blot. Results The growth of MDA-MB-231 cells infected with Ad-Noggin is faster than control group（P<0.05）, while scratch repair time extended. Transwell cell invasion assay shown that the invasive ability of MDA-MB-231 cells were inhibited in Noggin group(79±4), compared with GFP group(135±7)（P<0.05）. Using real-time PCR and western blot, the expression of CXCR4 and MMP1 was confirmed to decrease. Conclusion Noggin protein can enhance the proliferation of MDA-MB-231 cell line, and inhibit its invasion and metastases. Maybe the cancer-related gene CXCR4 and MMP1 was involved in it.

The genetic variations of the Bone Morphogenetic Protein 7 gene is associated with essential hypertension in Xinjiang old Uygur population

2011, 31(11):  1261-1266. 

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Objective To analyze the association between the genetic variations of functional region in Bone Morphogenetic Protein (BMP7)gene and hypertension in Chinese Uygur individuals. Methods The all exons, segmental introns and the promoter regions of BMP7 gene were sequenced in 48 Uygur Chinese. Representative variations were selected according to the minor allele frequency (MAF) and linkage disequilibrium and genotyped using the TaqMan polymerase chain reaction method in 1446 Uygur Chinese and a case-control study was conducted to test the association between genetic variations of BMP7 gene and hypertension Results 5 novel and 8 known variations in the BMP7 gene were identified. All genotype distributions were tested for deviations from Hardy-Weinberg equilibrium (P>0.05). There were significant differences of genotype distribution of rs17480735 between hypertension and control groups(P<0.05)．And the means of blood pressure in individuals with GA + AA were significantly higher than in individuals with GG genotypes of rs17480735 in population with age ≥50 years (P<0.05). The logistic regression analysis showed that AA genotype of rs17480735 variation might be a hypertensive risk factor in individuals with age ≥50years (P<0.05) after affect factors(age, gender, BMI, drinking and smoking) were adjusted. Conclusions The present study suggests rs17480735 polymorphism in the BMP7 gene may be associated with Hypertension in Uygur individuals with age ≥50 years .

Ex vivo activated macrophages myocardial transplantation improve the ventricular remodeling of the infarcted heart induced by ischemia/reperfusion

He LI

2011, 31(11):  1267-1272. 

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Objective To investigate the mechanism of the paracrine effect of phenytoin (PHT) and macrophage in the angiogenesis and ventricular remodeling after myocardial infarction (MI). Method The peritoneal macrophages were adherently cultured, and parts of cultured macrophages were pre-stimulated by PHT. The survival animals induced experimental ischemia-reperfusion (I/R) were randomly distributed into Sham group, control (AMI) group, Phenytoin (PHT) group group, macrophage (MΦ) group, phenytoin pre-stimulating macrophage (MΦ-PHT) group. At the 7th and 28th day after the induction of I/R, histology examination were analyzed including index of expansion, fiber area, arterioles density, capillary density in infarct region and cardiocyte cross-sectional area (CSA) in non-infarct zone. The levels of protein expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) within infarct region 7 days after MI were determined by Western blot. Result Compared with AMI group, Index of Expansion, fiber area and CSA in non-infarct zone at 28th day were all decreased in PHT group, MΦ group and MΦ-PHT group (P <0.05), while arteriole density in infarct zone had increased (P <0.05). Fiber area of MΦ-PHT group at 28th day was fewer than MΦ group (P <0.05). Compared with MΦ group, capillary density and arteriole density in infarct zone at 7th and 28th day had increasing tendency. The levels of VEGF, bFGF protein expression in infarct zone at 7th day in MΦ-PHT group were more than MΦ group (P <0.05). Conclusion Phenytoin may promote angiogenesis in infarct zone and improve ventricular remodeling after MI through stimulating macrophages, which up-regulate the growth factors involved in the angiogenesis through the paracrine effect.

Oxytocin induced rat bone marrow mesenchymal stem cells differentiate into cardiomyocyte-like cells in vitro

2011, 31(11):  1273-1277. 

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[Abstract] Objective To investigate the feasibility of inducing rat bone marrow mesenchymal stem cells(MSCs) differentiating into cardiomyocytes by Oxytocin(OT). Methods SD rat MSCs were isolated and cultured from rat bone marrow, then induced by OT for 72 h. The cultured cells were observed by phase-contrast microscope. The laser scanning confocal microscope (LSCM) was used for the expression of desmin, α-sarcomeric actin and C-TnT. GATA-4 and α-MHC expression was detected by relative quantitative RT-PCR after 7, 21, 28 days of induction respectively. Results The cultured cells grow stablely and proliferate faster, mainly appeared as fusiform and fibroblastic shape. After induced by OT for one week, most cells became spindle-like and pillar-form with oval nuclei lying in the center of cells and similar to cardiomyocytes. The direction of the cell arraying was similar gradually. After four weeks, a majority of cells enlarged,lengthened，showed stick-like shape and paralleled closely.The direction of the cell arraying was similar gradually. MSCs induced by OT could be identified by the positive staining for desmin, α-sarcomeric actin and C-TnT. laser scanning confocal microscopy indicated that desmin(α-sarcomeric actin ) was signed red, cTnT was green.When they were both been showed,it changed to yellow.RT-PCR assessment showed that the differentiated cells began to express GATA-4 from day 7 to day 28 of differentiation and began to express α-MHC from day 21 to day 28 of differentiation. Conclusion　MSCs can differentiate into cardiomyocytes in vitro after induced by OT.

Establishment of a simple and efficient method for isolating and culturing vascular endothelial cells of rat thoracic aorta

2011, 31(11):  1278-1282. 

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Abstract: Objective To establish a simple and efficient method for isolating and culturing vascular endothelial cells of rat thoracic aorta. Methods Under aseptic condition, the thoracic aorta was harvested from healthy male Wistar rat. After stripping off the adventitia, the thoracic aorta were cut into vessel rings with length of 1.0～1.5mm. The vessel rings were placed on culture dish vertically and filled with the culture medium DMEM/ F12 containing 10 % fetal bovine serum; After 24h, the vessel rings had attached on the culture dish firmly and some new culture medium were added; 3～4d later, the thoracic aorta was discarded and the new culture medium was added into the culture dish. The migrating cells were digested by 0.25 % pancreatic enzyme for serial subcultivation. The endothelial cells were identified by morphological, immunohistochemical and flow-cytometry methods with anti-vWF antibody, which was regarded as the marker of endothelial cells. Results A amount of cells migrated from the aorta and adhered to the bottom of culture dish 3～4d after cultivation. The confluent cells grew rapidly after being digested with 0.25% pancreatic enzyme and showed the typical cobblestone appearance. The cells were identified as endothelial cells, its positive ratio was (98.3±0.2)% by using immunostaining and flow-cytometry with vWF. Conclusion The modified explanting cultivation method for isolating and culturing was established in the this study. This method is simple, easy to handle and more economical and applicable as does not need collagen and endothelial cell growth promoting substrate. In addition, this modified method is especially suitable for isolation and cultivation of vascular endothelial cells from small arteries.

Study on 25 Cases of Urinary Negative Stones

2011, 31(11):  1285-1286. 

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Objective In order to seek the methods in the prevention and cure of urinary negative stones, the compositions of stones and the zeta potential of their urinary crystallites were analyzed. Methods Twenty-five negative stone samples were investigated by XRD and FT-IR. The zeta potential of urinary crystallites in lithogenic urines were investigated by nanoparticle size analyzer and were compared with 30 samples of healthy subjects. Results 24 cases of the negative stones mainly consisted of uric acid in which 6 cases containing a small quantity of calcium oxalate. Only one sample was ammonium urate. The urine pH of the negative stone formers, with an average of 5.25±0.26, was lower than that of the healthy subjects with an average of 6.06±0.41. The Zeta potential of urinary crystallites in 25 lithogenic patients was -5.04±3.48 mV, which being higher than that in normal subjects (-10.27±1.35 mV). Conclusions The urine pH of urinary negative stone formers was lower than that of the healthy subjects, but there is on difference between them (P>0.05). The Zeta potential of urinary crystallites in negative stone formers (with an average of -5.04±3.48 mV) was higher than that in healthy subjects (with an average of -10.27±1.35 mV), there is difference between them (P<0.05).

Relationship Between HIF-1α Expression and Angiogenesis in Non-small Cell Lung Cancer

2011, 31(11):  1287-1288. 

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Abstract Objective To investigate the expression and the relationship of angiogenesis of HIF-1αin non-small cell lung cancer(NSCLC).Methods 　Immunohistochemical method was employed to detect the expression of HIF-1αand angiogenesis in tumor and laterotumor tissue of 55 patients .The vascular endothelial cell was marked in CD105. Results The positive rate of HIF - 1αin NSCLC tissue was 63.3%，and MVD was 23.12 ±7.22 , which were higher than those in the laterotumor tissue ( P < 0. 05). The expression of HIF－1αwas positively correlated with the angiogenesis , TNM stage and lymphatic metastasis. Conclusion It is suggested that HIF-1α is involved in the tumor angiogenesis in NSCLC.

Research on Physiological and Pathophysiological Functions of X- box Binding Protein

张霞丽 ZHANG Xia-Li

2011, 31(11):  1289-1292. 

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ABSTRACT:X-box binding protein 1 is an important component of unfolded protein response.XBP-1 has two splicing variants that were designated XBP-1S and XBP-1U. The former activity is much higher than the latter. When the cell is on the condition of endoplasmic reticulum stress, XBP-1 is induced by ATF6,and spliced by IRE1,then the functional XBP-1S was transcripted, and activation of unfolded protein response. XBP-1 is an important transcription factor,it has an important regulatory role on cell growth and differentiation.XBP-1 is involved in the genesis,development of many diseases.

The metabolism and influence factors of skin melanin

2011, 31(11):  1293-1297. 

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The skin color of human is diversity, there is a direct relation between the number, magnitude, type, distribution of melanosome in melanocytes. The synthesis of melanin and the intercellular movement of melanin are complex and a precise process that determines the distribution and skin pigmentation. There are many factors influence the metabolism of skin melanin, that is tyrosinase, peroxidase, microelement, ultraviolet rays, structural proteins 17, protein receptor 2.