Table of Content

20 December 2009, Volume 29 Issue 12

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研究论文

Transplantation of bone marrow-derived mesenchymal stem cells improves acute lung injury in mice induced by LPS

Lei SUN; Xiao-ming WANG; Qin SI; Xiao-hong YU; Yu LIN; Jin QIU; Heng-yi GUO; Qi-xia WU

2009, 29(12):  1233-1238. 

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Objective To study the potential of transplanted bone marrow-derived mesenchymal stem cells (MSCs) in treating and repairing the acute lung injury in animal models. Methods MSCs were isolated from mouse bone marrow of healthy donors,cultrued and amplified in vitro. The lipopolysaccharide (LPS) was inhaled along postnasal tract to produce acute lung injury in mice and the MSCs labeled by Brdu were administrated via vein into the mice. The migration and differention of the cells were identified by immunostaining and double immunostaining. The pathological changes, pulmonary edema index and the content of IL-1β in lung homogenate were used to accese the therapeutical effect of MSCs. Results The cultured MSCs dispalyed a positive CD44 and a negative CD34.The Brdu-labeled cells were detected in the lungs of the recipient 4 days after transplantation, indicating its origin of MSCs.Theses cells also exhibited characteristics of aveolar epithelials,expressing the cytokeratin-the marker of epithelium. Compared with the injuried ones, the mice under the therapy with MSCs showed a decreased pulmonary edema index and IL-1β content in the lung homogenate. Conclusion These data suggest a therapeutical effects of MSCs in treating and repairing the mouse acute lung injury.

VEGF-induced Expression of Survivin in Differernt Types of Cells

Xue-hu WANG; Zhong-xue FU; Wei SHEN; Xing-ye WU

2009, 29(12):  1239-1243. 

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Objective To investigate the effects of VEGF-induced expression of Survivin in colon cancer cells (LOVO, HCT116) and human umbilical vein endothelial cells (HUVECs). Methods VEGF was used to stimulate the LOVO, HCT116, HUVECs. We used RT-PCR and Western blot to estimate the expression of Survivin in different cells, and immunocytochemistry to investigate the expression level and site of Survivin protein. Results Survivin was expressed highly in the cytoplasm and was upregulated with the induction of VEGF. In HUVECs, Survivin expression was not obvious but significantly upregulated with the inductioin of VEGF in the cytoplasm and nucleus. Conclusion The expression of Survivin mRNA and protein are upregulated by VEGF stimulation. The level and distribution of Survivin are different in tumor cell lines and non-tumorgenicity cells.

Comparison in Different Rabbit Models with Diastolic Heart Failure and Systolic Heart Failure

Lei WANG; Hai-peng WANG; Cai-ming ZHAO; Lian-hua HAN; Cao ZOU; Zhi-hua LIU; Wen-ping JIANG

2009, 29(12):  1244-1248. 

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Objective To compare the differences of cardiac function and interstitial remodeling between DHF and SHF rabbit models. Methods To establish DHF model with abdomial aorta ligation and SHF model with abdomial aorta ligation plus catheterization abdomial aorta ligation and catheterization. The cardiac function was detected by UCG parameters and homodynamic parameters. The collagen content was detected through hydroxyproline colorimetric assay and collagen area(CA), collagen volume fraction(CVF) and area ratio ofⅠto Ⅲ type collagen with PSR. Result Compared with control group, there were significantly increasing thickness and stiffness of myocardium, impaired diastolic function but normal ejection fraction (EF), and significantly increasing collagen content, CA, CVF and area ratio ofⅠto Ⅲ type collagen in DHF group; there were significantly enlarging heart chamber, decreasing systolic function, and significantly increasing collagen content, CA, CVF but decreasing ratio ofⅠto Ⅲ type collagen in SHF group (P<0.05 or P<0.01). Conclusion DHF and SHF rabbit models were established successfully, which can represent different clinical types and provide technical support to future research..

Construction and identification of a lentiviral vector harboring RNAi based on gene NSBP1

Ning JIANG; Li-qun ZHOU; Dian-qi XIN; Tian-jing LV; Wen-ke HAN

2009, 29(12):  1249-1253. 

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Objective: To construct and identify a lentiviral vector harboring RNAi sequence targeting NSBP1 gene. Methods: Three siRNA targeting the NSBP1 mRNA were designed, and the pGCSIL-GFP-NSBP1 lentivirus vectors were constructed, confirmed by DNA sequencing. A total of 293T cells were co-transfected with pGCSIL-GFP-NSBP1，pHelper1.0 and pHelper2.0 for the virus stocks produced, the titer of the virus was tested. After lentivirus were transfected into DU145 cells, Western-blot and MTT methods were used to determine the expression and biological activity of NSBP1 gene, the cells were transplanted into nude mice, then inhibitive effect was observed. Results: PCR analysis and DNA sequencing demonstrated that the RNAi sequence targeting the human NSBP1 gene was successfully inserted into the lentiviral vector. The titer of the recombinant lentiviral vector as 2×108TU/ml. NSBP1 protein expression level in transfected cells was significantly decreased and growth rate of cells transfected with lentivirus was decreased by MTT assay, the downregulation of NSBP1 reduced growth rate of transplantated tumor, whereas tumorgenicity was not influenced. Conclusion: The construction of the lentiviral vector of NSBP1 has been successfully prepared and NSBP1 plays an important regulatory role in androgen-independent prostate cancer cell proliferation.

Effects of repeated hypoxic exposures on glycolysis, mitochondrial oxidative phosphorylation and energy charge in the mouse brain

Xin LI; Sheng-nan XIE; Juan-juan YIN; Guang-wei LIU; Yao-hua LI; Guo-wei LV; Shun YU

2009, 29(12):  1254-1257. 

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Objective To study the effects of repeated hypoxic exposures (HEs) on glycolysis, mitochondrial oxidative phosphorylation and energy charge in the mouse brain. Methods Adult Balb/c mice were repeatedly exposed to hypoxia for 5 times and the standard tolerant time and body temperature were recorded. The activities of PFK, PK and mitochondrial complex I in the brain were assayed. Phosphoadenosines and energy charge were measured. Results Repeated HEs prolonged the hypoxic tolerance and reduced the body temperature. The activities of PFK and PK experienced regular changes, with an increase in 1st and 3rd HEs and a decline to control levels in 5th HE. The complex I activity continued to decrease during HEs. The energy charge was kept stable. Conclusion REs lead to a regular change in the activity of glycolysis, a continued inhibition of mitochondrial oxidative phosphorylation, and a maintained energy charge in the brains of mice.

Neural cell induction of human placenta derived adherent cells

Yue-si WANG; Fan-gang MENG; Yan-wei WANG; Jian-yuan LI

2009, 29(12):  1258-1262. 

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Objective: The expression of hematopoietic stem cell and neural markers on placenta adherent cells and their association were investigated, the potential of neural differentiation of placenta adherent cells so as to demonstrate the relationship between placenta adherent cells and hematogenesis and neurogenesis. Methods: Isolated adherent cells in human placenta tissue were stained immunocytochemically with Nestin,MAP2,MBP,CD133,CD34 antibody to detect protein production and induced neural differentiation with β-mercaptoethanol and RA. Results: Isolated placenta adherent cells which were confirmed to be heterogenous expressed CD133, Nestin, MAP2 weakly and CD34 none. While induced cells showed neural like morphology, strong positive for Nestin、MAP2 and weak positive for MBP and GFAP in immunocytochemical staining. Conclusion: Human placenta adherent cells can be induced into neural like cells in vitro which has quality characteristics of MSC,it provides a new source for clinical application of hMSCs．

Construction and expressionof secretary recombinant adenovis vectors carrying mPSMA

Dong-mei ZHANG; Shao-jun WANG; Xing-chen PENG; Li YANG

2009, 29(12):  1263-1267. 

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Objective To construct secretary recombinant adenoviral vector carrying the mouse prostate-specific membrane antigen gene through AdEasy vector system and the immune result was evaluated. Methods mPSMA was amplified from plasmid pCR-BluntII-TOPO by PCR and subcloned into transfer vector pAdenoVator CMV5, The signal peptide DNA sequence of hIL-2 was fused to 5′terminal of mPSMA gene to construct a secretary Ad-mPSMA. pAdv-mPSMA was co-transformed with pAdenoVator ΔE1/E3 through homologous recombination. The recombinant adenoviruses were packaged ,amplified and purified in HEK293 cells. HeLa cell was infected by recombinant adenovirus Ad-mPSMA and the expression of mouse prostate-specific membrane antigen gene was detected by RT-PCR and Western blot. The recombinant adenovirus had been immuned mice, sera antibody against mPSMA from immunized mice was detected by ELISA.Results The secretary pAd-mPSMA was constructed successfully and typical cytopathic effect (CPE) was observed. The titer of the recombinant adenovirus was 1.32x1011IU/ml and expression of mPSMA was confirmed by RT-PCR and Western blot. The specific antibody against mPSMA had been found in serum of the immunized mice. Conclusion Secretary mPSMA gene recombinant adenovirus was constructed successfully, which provide a basis for further study on the anti-tumor immunotherapy role of PSMA.

Expression and biological function identification of TCR 9/ 2-Fc protein in baculovirus vector expression system

Yang GUO; Jing ZHENG; Yu HU; Lian-xian CUI; Wei HE

2009, 29(12):  1268-1272. 

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Objective To establish an expression system of TCR 9/ 2-Fc protein by baculovirus vector expression system and identify biological function of expressed TCR 9/ 2-Fc protein. Methods Fc and Fc gene fragments were amplified by overlap PCR and inserted into expression vector pBACp10ph. The recombinant plasmid pBACp10ph- 9/ 2(OT3)-Fc and the baculovirus DNA were co-transfected into sf9 cells. The expression position of TCR 9/ 2(OT3)-Fc was identified by Western blot and the expression efficiency of 9Fc and 2(OT3)Fc was tested by FCM. Furthermore, the binding activity of TCR 9/ 2(OT3)-Fc protein with SKOV3 cells and MNS protein was evaluated with confocal and SPR. Results The recombinant vector pBACp10ph- 9/ 2(OT3)-Fc was constructed and TCR 9/ 2(OT3)-Fc protein was expressed in sf9 cells. However, the expression efficiency of 9Fc and 2(OT3)Fc was quite different. It was proved that purified TCR 9/ 2(OT3)-Fc protein can bind with SKOV3 cell and MNS protein. Conclusions TCR 9/ 2-Fc protein is successfully expressed in baculovirus vector expression system and TCR 9/ 2-Fc protein can mimic the binding activity of TCR in vitro

Hypoxic preconditioning up-regulates the activity and gene expressions of glucose transporters (GLUT1 and GLUT3) under anoxic condition in cultured rat hippocampal neurons and astrocytes

Er-fu WANG; Xin LI; Chun-song JIA; Guang-wei LIU; Hai-xia FANG; Shun YU

2009, 29(12):  1273-1276. 

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Objective To explore the effect of hypoxic preconditioning on the activity and gene expressions of glucose transporters in the cultured rat hippocampal neurons and astrocytes under anoxic condition. Methods The cultured rat hippocampal neurons and astrocytes were preconditioned for 6 days by intermittently exposing to hypoxic gas mixture (1% O2, 10% CO2, 89% N2) for 20 min each day. 24h after the last hypoxic exposure, the cells were exposed to anoxic gas mixture (10% CO2, 90% N2 ) for 6h, and the uptake rate of [3H]- 2-deoxyglucose (2-DG), the levels of glucose transporter GLUT1 and GLUT3 mRNAs and the cell survival rate were examined immediately after anoxic exposure. Results Neurons and astrocytes preconditioned with hypoxia showed higher 2-DG uptake rates and increased expressions of GLUT1 mRNA in the astrocytes and GLUT1 and GLUT3 mRNA in the neurons. The preconditioned neurons also presented increased tolerance to anoxia, which was abolished by cytochalasin B, a specific inhibitor of glucose uptake. Conclusions Hypoxic preconditioning up-regulates the activity and gene expressions of glucose transporters of hippocampal neurons and astrocytes under anoxic condition.

Screening of the target genes related to the development and progression of ESCC

Pei LI; Zhi-qiang LING; Hong-yan YANG; Ji-min ZHAO; You-tian HUANG; Ming-yao ZHAO; Zi-ming DONG

2009, 29(12):  1277-1281. 

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OBJECTIVE: This study was to investigate the differentially expressed genes between primary esophageal squamous cell carcinoma and normal esophageal mucosa using cDNA microarray. METHOD: LCM-GMA-cDNA microarray was used to detect the mRNA from both the primary carcinoma and the corresponding esophageal epithelium in 15 cases of human esophageal squamous cell carcinoma (ESCC). After high-stringent washing, the cDNA microarray was scanned for the fluorescent signals and showed differences between 2 tissues. RESULTS: Among the 886 target genes, 34 genes had significant differed between the Ⅰ/Ⅱand Ⅲ/Ⅳ group. Cell cycle regulators possibly promoting the growth of tumor cells were highly expressed in the early stages of ESCC, whereas adhesion molecules and extracellular matrix-related molecules possibly promoting invasiveness increased in the later stages. CONCLUSION: More than one gene contributed to esophageal cancer and the profiles of genes expression will open up new possibilities for understanding the molecular mechanism of tumor progression and be helpful to clinical treatment.

Valproic acid Inhibits the cardiomyocyte hypertrophy induced by angiotensin Ⅱ in rats

Ying LU; Shuang YANG; Wen-juan DU

2009, 29(12):  1282-1285. 

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Objective To observe the inhibitory role of Valproic acid (VPA) in hypertrophic cardiomycytes induced by angiotensin Ⅱ（AngⅡ）in rats. Method The cultured rat cardiomyocytes were divided randomly into the control group, the hypertrophic group and VPA group. They were treated with AngⅡ to induce hypertrophy and then given with VPA. The morphologic changes of cardiomyocytes were observed under the contrast phase microscope and electron microscope. The mRNA levels of β-MHC were exmained by Reverse transcriptase polymerase chain reaction (RT-PCR) method.The protein expressions of c-fos were exmanined by immunohistochemistry method. Result Stimulated by AngⅡ,the cardiomyocytes were enlarged under the contrast phase microscope and the ultrastructure also changed. After stimulated by AngⅡ,the mRNA level of β-MHC and the protein expression of c-fos increased in the hypertrophic cardiocytes. Given by VPA, these data decreased accordingly. Conclusion VPA may inhibit the cardiomyocyte hypertrophy induced by angiotensin Ⅱ in some degree.

Construction of a siRNA Expression Plasmid Targeting CXCR4 and Its Effect on the invasion ability of the breast cancer cell

Jun-fei FENG; Jian DONG; Min HONG; Chang-e GAO

2009, 29(12):  1286-1290. 

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Objective To construct and identify the siRNA eukaryotic expression vector targeting gene CXC chemokine receptor-4 and study its role in invasion ability of bread cancer cells. Methods Two siRNAs were designed and synthesized according to the coding the cDNA sequence of CXCR4 gene and cloned into eukaryotic expression plasmid pGE-1-U6/kna.The constructed CXCR4-siRNA expression vector was transfected into MDA-MB-231 cells by liposome.Western blotting were used to evaluate the suppression of CXCR4 expression in different groups. The invasion and migration of MDA-MB-231 cells were evaluated by cell invasion assay in vitro. Results　Enzyme digestion and DNA sequencing confirmed that the CXCR4-siRNA expression vector was constructed successfully. After transfection,the CXCR4-siRNA obviously suppressed the expression of CXCR4 compared with control groups and the ability of cell migration was decreased markedly. Conclusions CXCR4-siRNA expression vector can effectively suppress CXCR4 expression in the breast cancer cell and decrease potential of cell invasion, which may provide a novel applicable strategy for gene therapy of breast cancer metastasis.

Puerarin Reduces Ethanol-induced-Apoptosis of Spermatogenic Cells in Rat Testis

Hong-yun HE; Yi-hao DENG; Xin-wen YANG; Kai-ming YANG

2009, 29(12):  1291-1295. 

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0bjective To investigate whether puerarin could protect against ethanol-induced apoptosis of spermatogenic cells in rat testis．Methods 30 S.D adult male rats，were randomly divided into three groups: normal control group ,alcohol group and puerarin group. At the 40 day， Bcl-2 and Bax of spermatogenic cells of testis tissue was checked by RT-PCR and immunohistochemistry; Apoptosis of spermatogenic cells was determined by TUNEL. Results The results of RT-PCR and immunohistochemistry indicated that Bcl-2 and Bax of spermatogenic cells were no significant statistical difference between group B and group C(P>0.05)，but there was significant statistical difference between group A and group B (P<0.01). Apoptosis of spermatogenic cells in group A were significantly higher than group C. Conclusion Spermatogenic cells could generate apoptosis by changing the expression of Bcl-2 and Bax. Puerarin could inhibit this damage of didymus by alcohol.

The Relationship of Polymorphism of Alpha2-HS glycoprotein and Atherosclerosis and Osteoporosis in Elderly Female

Jian-li LIN; Jian-ming HOU; Qing-ming LIN; Li-xiang LIN; Wei-te ZHUANG; Fa-qiang TANG; Long JIN

2009, 29(12):  1296-1300. 

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Objective To investigate the distribution of Alpha2-HS glycoprotein ( AHSG) gene polymorphisms and the relationships of AHSG gene polymorphisms with atherosclerosis and serum bone relative biochemical markers. Methods Blood samples of 342 patient-in female patients, aged 20～80, were collected serum bone alkaline phosphatase, cross-linked N-telopep tide of collagen typeⅠ, cross-linked C-telopep tide of collagen type Ⅰ, osteoprotegrin and leptin were determined by ELISA. Serum TC、TG and calcium content were detected.Polymorphisms of AHSG gene were detected by polymerase chain reaction fragment length polymorphisms ( PCR-RFLP) of restriction enzyme Sac I. BMD (Norland XR-36) of the anteroposterior spine (AP) , supine lateral spine (Lat) , and femoral neck ( FN) were measured. Morphological changes in the aorta and bone of type GG patient were detected by pathological section. IMT were measured by Color Doppler Ultrasound equipment（SEQUOIA512）.Results　(1) The genotype frequency of CC, CG, and GG were 59.7% , 25.1% and 15.2% respectively in all elderly females patients.There were significant differences in blood lipids，Ca2＋ and serum bone relative biochemical markers to different AHSG genotypes. (2)There were significant differences in the BMD of the AP, Lat, FN and IMT and the serum biochemical markers among the CC，CG and GG genotypes. (3)GG-female patients bone tissue pathology section verify the AHSG polymorphism genetic mutation and atherosclerosis, osteoporosis development of the relationship.Conclusions There were closely related among AHSG polymorphism variation and the incidence of arteriosclerosis and osteoporosis in elderly female.

Association of apolipoprotein E gene polymorphism with postoperative delirium

Gang TAN; Xiang-yang GUO; Ai-lun LUO; Yu-guang HUANG; Jian-qing XU; Hong-zhi REN; Tie-hu YE

2009, 29(12):  1301-1304. 

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Objective To investigate whether apolipoprotein E (APOE) genotypes associate with postoperative delirium in aged noncardiac surgical patients. Methods 212 inpatients, aged 65yr or older, undergoing selective noncardiac surgeries were enrolled in the study. The patients were frequently interviewed and evaluated prospectively for delirium with the Confusion Assessment Method (CAM) during the first three postoperative days. APOE genotype was determined using multiplex amplification refractory mutation system polymerase chain reaction(multi-ARMS PCR) technique. Results Delirium occurred in 45 patients during the first three postoperative days. Of the 212 patients, 18 (8.5%) possessed one or two APOE ε4 allele. There was no significant difference between delirious patients and non-delirious patients(6.7%: 9.0%, P>0.05) in the presence of APOE ε4 allele. In all four APOE ε4/4 homozygote patients, one female patient presented a transient delirium status at three days before surgery, another male patient presented serious fluctuated delirium symptoms from the second to 17th days postoperatively. Conclusion The presence of APOE ε4 allele may not be a predictator of postoperative delirium. APOE ε4/4 homozygote patients may be more indulgent to delirium than others.

Expression and significance of PDGF-D and VEGF in gastric carcinoma

Lin ZHAO; Cai-quan ZHANG; Gang LIAO; Jie-gen LONG; Wei-xue TANG

2009, 29(12):  1305-1309. 

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Objective To investigate the expression of Platelet-derived growth factor-D （PDGF-D） and Vascular endothelial growth factor（VEGF） in gastric carcinoma and elucidate the relationship between their overexpression and clinical pathological characteristics. Methods Immunohistochemical assay was performed to detect the protein expression of PDGF-D and VEGF in tissues. Semiquantitative reverse transcription polymerase chain reaction (RT-PCR) was performed to evaluate the mRNA expression of PDGF-D and VEGF in part of random selecting gastric carcinoma samples. The correlation between the expression of PDGF-D and VEGF, and the relationship between the expression of PDGF-D, VEGF and the clinical pathological characteristics were analyzed. Results The protein expression of PDGF-D and VEGF in gastric carcinoma tissues were significantly higher than that in adjacent tissues and normal gastric mucosa(P<0.05 )；The expression of PDGF-D and VEGF correlated with TNM staging, depth of invasion ，and lymphatic metastasis (P<0.05), while the expression of PDGF-D also correlated with histological differentiation (P<0.05). There was a significant positive correlation between PDGF-D and VEGF at the mRNA and protein expression levels. (P<0.05). Conclusions PDGF-D and VEGF specifically highly expressed in gastric carcinoma. The abnormal expression may play an important role in the carcinogenesis and metastasis of gastric carcinoma.

ASPIRIN INHIBITE THE PROLIFERATION OF RAT CARDIAC FIBROBLASTS INDUCED BY ENDOTHELIN-1

Zhi-guo ZHANG; Li YANG; Ya-ping ZHENG; Yan-feng ZHAO

2009, 29(12):  1310-1313. 

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Aim: To investigate the effect of Aspirin on endothelin-1 induced proliferation of cardiac fibroblasts (CFs). Methods: Isolated and cultured CFs from neonatal Sprague-Dawley rats (SD) were randomly divided into 7 groups: ①control, ②ET-1, ③Aspirin, ④ET-1+ Aspirin 1mmol/L, ⑤ET-1+ Aspirin 2mmol/L, ⑥ET-1+ Aspirin 5mmol/L and ⑦ET-1+ Aspirin1 10mmol/L. CFs were counted by MTT assay. Cell cycle distribution was determined with a flow cytometer (FCM). [3H]-Proline incorporation was evaluated by scintillation counting. Nitric oxide (NO) was measured by colorimetry. Results: 10-7mol/L ET-1 significantly increased the number of CFs and [3H]-Pro incorporation and decreased NO2-/NO3- secretion compared with the control group (P < 0.01). 1-10mmol/L Aspirin inhibited the effects of ET-1 on CFs in a concentration-dependent manner (P < 0.05 vs ET-1). In the ET-1 group, the percentage of cells in the S phase was higher than control, which was inhibited by 10mmol/L Aspirin (P< 0.01 vs ET-1 and control). Conclusion: Aspirin can restrain the proliferation and collagen synthesis of cardiac fibroblasts induced by endothelin-1, and this effect may be partially mediated by NO.

Impaired cholesterol efflux in macrophages of patients with type 2 diabetes mellitus is associated with the decreased expression of ABCG1

Hui-juan WANG; Lian-feng CHEN; Quan FANG; Xiao-wei YAN

2009, 29(12):  1314-1319. 

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Objective In the present study, we examined the cholesterol efflux and the expressions of ATP-binding cassette receptor A1 (ABCA1), ABCG1 and scavenger receptor B1 (SR-B1) in monocytes derived macrophages of patients with type 2 diabetes mellitus. Methods and Results Blood was collected from subjects with and without Type 2 diabetes mellitus. Peripheral blood monocytes were differentiated for 72 hours into macrophages, and cholesterol efflux assays, Real-time quantitative PCR and western blot were performed. Macrophages from patients with type 2 diabetes mellitus had a reduction in cholesterol efflux relative to healthy controls. The mRNA and protein expressions of ABCG1 in macrophages from patients with type 2 diabetes mellitus were significantly reduced compared with control subjects. In contrast, the expression of ABCA1 and SR-B1 were similar in both control subjects and diabetic patients. In addition, cellular cholesterol efflux from macrophages to autologous serum and pool serum was significantly correlated with the expression of ABCG1. Conclusion ABCG1 expression and cholesterol efflux are reduced in patients with type 2 diabetes mellitus. This impaired cholesterol efflux significantly correlates with decreased expression of ABCG1.

研究短文

Effects of sodium valproate on blood lipids and thyroid gland function in adult epilepsy

Xue-dong SUN; Yi-feng DU; Zhao-fu CHI; Wei WU

2009, 29(12):  1320-1321. 

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Rosiglitazone increased the expression of pigment epithelium-derived factor in STZ-induced diabetic rats

Jian-hua PANG; Ya-ru ZHOU; Shan LIU; Shu-chang WANG; Qing-fang SONG; Zhan-jian WANG; Kuan-zhi LIU

2009, 29(12):  1322-1324. 

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Effect of tetramethylpyrazine on oxygen free radical in rats with acute severe pacreatitis

Fu-jun YAO; Yu-peng LIU; Shuang-cheng LI

2009, 29(12):  1325-1326. 

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Establishment and clinical application of real-time PCR for detecting pathogenic fungus

Hai-ping ZHANG; Li-jia YANG; Shi-qin TAO; Ying-wei ZHAO

2009, 29(12):  1327-1328. 

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Clincial evaluation of insulin glargine in type 2 diabetes mellitus

Bing WANG; Jun MA

2009, 29(12):  1329-1330. 

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短篇综述

Functions of P2 receptors in blood vessel and the relationship with some vascular diseases

Jia-ri LIN; Gui-lin LI; Shang-dong LIANG

2009, 29(12):  1331-1333. 

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P2 receptors are divided into two subclasses：P2X receptors which are ligand-gated ion channels and P2Y receptors which are G-protein coupled. The several kinds of P2X and P2Y receptor subtypes have been expressed in vascular endothelial cells and vascular smooth muscle cells. Purinergic signaling plays an important role in vascular diseases such as atherosclerosis, cerebral vessels ageing and blood vessel remodeling and makes it possible to provide a new target to treat vascular diseases.

Aquaporins and cancer

Hong ZHANG; Zhen-zhen LI; Zhi-yong WANG; Jian-guo WEN

2009, 29(12):  1334-1336. 

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The aquaporins (AQPs) are small, integral-membrane proteins that selectively transport water across cell plasma membranes. AQPs are strongly expressed in tumor cells of different origins, particularly aggressive tumors. AQPs-expressing cancer cells show enhanced migration in vitro and greater local tumor invasion, tumor cell extravasations, and metastases in vivo. AQPs inhibition will provide a help for titumor therapy.

ERS response mediate glucolipotoxicity-induced β-cell apoptosis

Yan-li YANG; Ruo-lan XIANG; Qi SUN

2009, 29(12):  1337-1340. 

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As one of the most sensitive cells of endoplasmic reticulum stress (ERS), pancreatic β-cells have an abundance of endoplasmic reticulum. Fatty acids cause apoptosis of β-cells and might contribute to β-cell loss in type 2 diabetes via the induction of ERS. Glucose is an amplifier of the ERS response to fatty acid, leading to increased β-cell apoptosis. ERS response is mediate to glucolipotoxicity-induced β-cell apoptosis.

医学教育

Practice of Problem Based Learning in Pathophysiology

Ruo-lan XIANG; Hai XU; Yuan-sheng GAO; Li-mei LIU; Li LI; Cheng WANG; Yan ZHANG; Li-lin WU

2009, 29(12):  1341-1343. 

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We has designed and carried out problem based learning (PBL) pedagogy since 2004．According to clinical cases, students learnt the pathophysiology of heart failure knowledge by themselves. Each group recommended one student to make an oral presentation and wrote a review about heart failure. Preparing clinical cases and group discussions are very important. At the same time we should pay attention to the change in role of the teacher in PBL and cooperation with other disciplines.