Table of Content

05 January 2010, Volume 30 Issue 1

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研究论文

Increased phosphorylation of CaMKⅡ in cerebral tissues of hypoxic preconditioned mice

Hai-tao LI; Jun JIANG; Wei-wei YANG; Xiang-ning BU; Song HAN;

2010, 30(1):  1-5. 

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Objective To explore the role of Calcium/calmodulin-dependent protein kinaseⅡ(CaMKⅡ) in the development of cerebral hypoxic preconditioning(HPC). Methods Healthy male BALB/c mice (weighting 18～22g, 8～10w) were randomly divided into 7 groups as follows: normoxic control (H0), early(H1-H4)and delayed (H5 and H6) hypoxic preconditioned mice groups. SDS-PAGE, Western blot and Gel Doc imagine systems were applied to quantitatively analyze the level of CaMKⅡ phosphorylation and protein expression level in the brain of mice. Results Compared with H0 group, the phosphorylation level of CaMKⅡ increased in cortex and hippocampus of mice in H3-H5 hypoxic preconditioned groups(P<0.05, n=6). However, there was no significant changes in total CaMKⅡprotein expression in cortex and hippocampus of hypoxic preconditioned mice. Similarly, enhanced p-Thr286 CaMKⅡwas also observed in the hippocampus and cortex of mice by immunostaining following hypoxic exposures (H3 and H6). Conclusion The increased phosphorylation of CaMKⅡ may involved in the development of cerebral HPC in mice.

IL -10 inhibits myocardium collagen deposition after acute myocardial infarction in rats

Xiao-ning HAN; Chun-yang HU; Song-yun CHU; Yong-fen QI; Wen-hui DING

2010, 30(1):  6-12. 

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Objective: To test the hypothesis that IL-10 might be useful for promoting left ventricular remodeling and cardiac function by modulating extracellular matrix after acute myocardial infarction. Methods: Male adult rats were divided into three groups in random: control group (n=6), MI/AAV2 group (n=16) and MI/AAV2-IL-10 group (n=16). Establishing animal modol of experimental myocardial infarction and equal dose of recombinant adeno-associated virus type 2 (AAV)/IL-10 (AAV2-rhIL-10) and AAV2 were injected around the ischemic zone. Echocardiography parameters, hemodynamic parameters, left ventricular mass index (LVMI), collagen volume fraction (CVF), perivascular circumferential area (PVCA), collagen type Ⅰ&Ⅲ volume fraction and mRNA levels of collagen type Ⅰ&Ⅲ, matrix metalloproteinases-2 (MMP-2) and tissue inhibitor of metalloproteinase-1 (TIMP-1) were compared between the three groups. Results: Improved cardiac function was observed in MI/AAV2-IL-10 group both by echocardiography and hemodynamic examination. 4 weeks after myocardial infarction, thickness of different parts of LV was same in MI/AAV2-IL-10 group and MI/AAV2 group. Nevertheless CVF, PVCA, and collagen type I volume fraction was significantly descending in remote zone of MI/AAV2-IL-10 group compared with that of MI/AAV2 group, P＜0.01. The mRNA expression of collagen type I and MMP-2 was lower in MI/AAV2-IL-10 group than that in MI/AAV2 group, P＜0.05 and P＜0.01 in turn. Conclusions: Recombinant IL-10 expression mediated by AAV2-rhIL-10 transfection of rats' myocardium contributes to promoting LV remodeling and cardiac function after acute myocardial infarction. The promotion was partially achieved by inhibition myocardium collagen deposition.

Establishment and their functional analysis of CHO cells with stable expression of hGHR gene and its mutants

Li-hua WU; Xiao-li CHEN; Wen-qin TANG; Ting ZHANG; Zhi-yong LIAO

2010, 30(1):  13-18. 

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Objective To construct three eukaryotic expression vectors containing wide-type hGHR gene and its mutants (hGHR-E42K、hGHR-H56R) related to Congenital Growth Hormone Insensitivity, then make sure these vectors are expressed stably in CHO cells. After hGH stimulation, we measured the expression of phosphorylated STAT5 in those cells. Methods With the available PUC-hGHR vector, other two mutate hGHR genes (hGHR-E42K,hGHR-H56R) were achieved by quick site-directed mutagenesis. Then three recombinants were cloned to eukaryotic expression vectors, pcDNA3.1/zeo(+) with restriction enzymatic reactions. Then with Lipofectamine2000, we transfected expression vectors to CHO cells and screened the stably expressed CHO cells by Zeocin. RT-PCR and/or Western Blotting were used to testify levels of hGHR and STAT5-P. Results After sequencing, two mutations were introduced to hGHR smoothly, three eukaryotic expression vectors identified. The transfected CHO cells expressed wt-hGHR or its mutants. Compared with hGH-wt, two mutate cells (E42K, H56R) had decreased phosphorylated STAT5 levels. Conclusion：Three CHO cells which stably expresses wide type hGHR and its mutants were successfully established, E42K, H56R partly interfered the phosphorylation of STAT5.

Effects of Allogenic Intra-bone Marrow Bone Marrow Transplantation on the Hematopoiesis in Mice

Ya-hong YUAN; Yong WANG; Zhen WENG; Yan DING; Dong-sheng LI

2010, 30(1):  19-23. 

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Objective To investigate the effects of allogenic intra-bone marrow bone marrow transplantation ( IBM-BMT) on re-establishing hematopoiesis in mice. Methods Bone marrow mononuclear cells (BMNCs) from BALB/c mice were transplanted into the C57BL/6 mice treated with a lethal dose of 60Coγ-ray radiation either by intra-bone marrow injection or intravenous injection. Sixty of the C57BL/6 mice were randomly divided into three groups as higher dose intra-bone marrow injection group (IBM1 group), lower dose intra-bone marrow injection group (IBM2 group) and intravenous injection group (IV group) with 20 in each group. The nucleated cell numbers of whole bone marrow from the tibia of each recipient mouse were counted respectively at the day 1, day 3, day 6 and day 9 after the transplantation. The numbers of donor-derived total nucleated cells and myeloid cells were further analyzed by flow cytometry. Results At 6th day after transplantation, the numbers of total bone marrow nucleated cells, total donor-derived nucleated cells and donor-derived myeloid cells in the tibia of injected side in both IBM1 group and IBM2 group were all significantly higher than those in IV group (P＜0.05 or P＜0.01). Conclusion Compared with traditional bone marrow transplantation (IV-BMT), IBM-BMT improves the bone marrow hematopoiesis in the early hematopoietic re-establishing stage in allogenic bone marrow transplantation.

Alleviative Effects of Lidocaine Postconditioning on Pulmonary Ischemia-Reperfusion Injury in Rats

Mao XU; Feng GAO; Xiang-yang GUO

2010, 30(1):  24-27. 

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Objective to investigate the alleviative effects of lidocaine postconditioning on pulmonary injury following ischemia reperfusion. Methods 72 adult SD rats were randomized to 4 groups (n=18 for the each): sham group, ischemia-reperfusion group, ischemic postconditioning group and lidocaine postconditioning group. The pulmonary ischemia-reperfusion model was established by occlusion of the left hilum of lung for 45min and the reperfusion was taken by removing the clamp for 2h. At the moment of reperfusion, lidocaine 4mg/kg was injected in as a priming dose following a continuous rate of 4mg/kg·h. PaO2, TNF-α, W/D of left lung, the level of MDA of left lung tissue were measured. At the end of reperfusion left lung was removed for microscopic determination. Results Following reperfusion PaO2 of lidocaine group was much higher than that of I-R group（P＜0.05）. Lidocaine postconditioning induced a significant decrease in the level of MDA of lung tissue（7.03±1.17μmol/L） compared with ischemia reperfusion group ( 8.77±1.42μmol/L）（P＜0.05）.Lidocaine postconditioning resulted in a lower level of TNF-α（1.69±0.34μg/L）than that of I-R group(2.52±0.54μg/L）（P＜0.05）. Microscopic examination showed that lidocaine postconditioning could decrease the level of edema of left lung and accumulation of neutrophils. Conclusion Lidocaine postconditioning exerts a protective effect on pulmonary ischemia-reperfusion injury administered in the beginning of reperfusion. The effect may be related to the antioxidant effect and the suppression of expression of TNF-α.

Salvia Miltiorrhiza Monomer IH764-3 Protects Human Brain Microvascular Endothelial Cell from Hypoxia/reoxygention Injury by Inhibiting Leukocyte Activity

Ran ZHOU; Wen-jian ZHANG; Jin-ning LOU; Li-ya YE; Cheng-hui LI

2010, 30(1):  28-32. 

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Objective To investigate the effect of IH764-3 on the leukocyte-mediated hypoxia-reoxygention injury of human brain microvascular endothelial cell (HBMVEC). Methods MTT assay was used to detect the survival of HBMVEC; gelatin zymography was used to analyze the activity of MMPs. The level of reactive oxygen species （ROS） in leukocyte was determined via commercially available kit, and the indirect enzyme-linked immunosorbent assay （ELISA） was used to quantify the contents of TNF-α, IL-1α, IL-2 and INF-γ in leukocyte culture medium. Results Survival of HBMVEC was impaired by hypoxia-reoxygenation, and aggravated by supernatant of activated leukocytes, which was attenuated by IH764-3. Leukocytes produced high levels of MMP-9, ROS and cytokines (TNF-α, IL-1α, IL-2, IFN-γ) after suffered hypoxia-reoxygenation, which was inhibited by IH 764-3. Furthermore, IH 764-3 could effectively reverse hypoxia-reoxygenation injury of HBMVEC with supernatant of activated leukocytes. Conclusion IH764-3 can protect HBMVEC against leukocyte-involved hypoxia-reoxygenation injury by attenuating the activation of leukocytes and inhibiting the injury effects of leukocytes products.

Image of pathology in rabbit unstable plaque with18F-FDG PET/CT detection

Dan-dan ZHANG; Zhan-min XU; Ai-li SONG; Quan-ming ZHAO; Xiao-li DONG

2010, 30(1):  33-36. 

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Objective To study the feasibility of noninvasive detection of unstable plaques with 18F-Fluorodeoxyglucose (18F-FDG) PET/CT imaging. Methods Atherosclerotic plaques were induced in male New Zealand white rabbits. Animals were injected with FDG labeled with 18F , then imaged with PET/CT . Aorta was explanted for photography with digital camera ,and 18F-FDG uptake analysis. 30 unstable plaques and 30 stable plaques were choosed so as to compare the quantitative 18F-FDG uptake.The number of macrophages and smooth muscle cells was detected by immunohistochemical technique. Results Experimental group showed inconsistent uptake of 18F-FDG in the abdominal aorta. The results were confirmed in the ex vivo digital photo of the explanted aorta. The target to non target ratio(T/NT) and macrophages of unstable plaques were higher than stable plaques(P<0.01), but smooth muscle cells obviously reduced (P<0.01).Correlation analysis showed that there was a positive correlation between T/NT and macrophage content( r=0.815，P<0.01), and a negative correlation between T/NT and SMC content(r=-0.684，P<0.01). Conclusion 18F-FDG PET/CT can constitute an attractive imaging method for the noninvasive detection of experimental unstable plaques.

NDRG2 gene transfection inhibition proliferation of breast cancer cell

Cai-lin ZHU; Nan-lin LI; Ting WANG

2010, 30(1):  37-41. 

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Objective:To explore the effect of gene NDRG2（N-myc downstream regulated gene 2） transfection on the proliferation and apoptosis in breast cancer cell. Methods : Bcap37 cell line express low level NDRG2 gene is transfected with recombinating adenovirus expressing high level NDRG2 gene to improve NDRG2 gene expression level. The NDRG2 expression level was detected by Western blot and the proliferation activity of the cell lines was detected by MTT and flow cytometry. Results : High expression level of NDRG2 inhibits cells growth and results in G1 phase arrest in Bcap37 cell line. Compared with non-transfected Bcap37 cells and Bcap37 cells transfected by empty vector, apoptosis cells obviously increase in Bcap37 cell line transfected by recombinating adenovirus expressing high level NDRG2 gene, 12% after 48 hours and 21.5% after 72 hours. Conclusions: Overexpression of NDRG2 in Bcap37 cells effectively inhibited cell proliferation and induced cell cycle arrest and apoptosis.

Therapeutic Effects of Mesenchymal Stem Cells Transplantation to Parkinson' s Disease Rat Models

Wen-yu FU; Zhi-juan ZHENG; E LV; Wen-xin ZHUANG; Xi-he SUN; Cui LV; Li YANG

2010, 30(1):  42-47. 

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Objective To investigate the therapeutic effects of rat bone marrow-derived mesenchymal stem cells(MSCs) transplantation into the corpus striatum of the 6-OHDA injection side of the Parkinson's disease(PD) rat models. Methods The BrdU labeled MSCs were transplanted into the corpus striatum of the 6-OHDA injected side of 6 model rats. Before MSCs transplantation and 8 weeks later, the ethological changes of PD rats were detected. After 2 days the concentrations of N-acetylaspartate(NAA), Choline(Cho) and Creatine(Cr) were calculated by 1H-MRS. Relative concentrations of NAA/Cr and Cho/Cr were recorded. The expression characteristics of tyrosine hydroxylase(TH) in substantia nigra compacta(SNc) were checked and the migration of the transplanted cells were observed using immunocytochemical and immunofluorescent methods. Results The rotational behaviors of PD rats improved, and there were statistical differences in the survival TH positive cells in SNc after the transplantion of BrdU labeled MSCs. The BrdU positive cells scattered in the transplantation side of the brain and showed GFAP positive and Map positive partly. Compared with before transplantation, The NAA/Cr radio of the 6-OHDA injected side of striatum was increased (P<0.05) and the Cho/Cr radio was decreased(P<0.05). Conclusion The transplanted MSCs can survive in the PD rat brain and has the therapeutic effect to PD.

Transplanting Marrow Mesenchymal Stem Cells can Improve the Ventricular Remodeling and Heart Functions to Acute MI Rat Models

Xu-xian WU; Zhi-xu HE; Xiang-chun SHEN; Zhong-jun ZHOU; Jian LIU

2010, 30(1):  48-53. 

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Objective To study the effects of marrow mesenchymal stem cells on heart functions and ventricular remodeling after myocardial infarction in rats. Methods Myocardial infarction was operated by ligation of the left anterior descending coronary artery ( LAD ) in adult SD rats. 4 and 8 weeks after MMSCs implantation, hemodynamic evaluations, left ventricular weight/body weight ratio and heart weight/body weight ratio were determined. HE staining was performed for double-blind counting the density of microvasculars and Van Gieson staining was used for measurements and calculation of the myocardial fibrillar collagen. Then we investigated the migration and evolution of MSCs in vivo by fluorescent microscope. Results First of all, HR were significantly decreased in transplantation MMSCs group. After transplantation 8 weeks, bodies weight in transplantation MMSCs group reached to control group's. At the same time, SBP, DBP and MBP, were significantly increased in transplantation MMSCs group. HR were significantly decreased in transplantation MMSCs group. Secondly, left ventricular weight/body weight ratio and heart weight/body weight ratio were significantly decreased after transplantation MMSCs 4 weeks. Then the ratio were significantly decreased after transplantation MMSCs 8 weeks. Thirdly, our results also demonstrated that the density of microvasculars were increased at the boundary of infarction site in the animals transplanted MMSCs. Finally, total volume of the myocardial fibrillar collagen was reduced in the MMSCs treated groups after MI. Conclusion Transplanting MMSCs can improve the ventricular remodeling and heart functions to acute MI rat models.

Effect of phosphorylated-p38MAPK on caspase-3 expression in substantia nigra of the MPTP mouse model of Parkinson's disease

Zi-feng WEI; Yong-sheng WANG; Qian WANG; Li-ren MA; Zuo-feng ZHANG; Yu-xin ZHANG

2010, 30(1):  54-58. 

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Objective: To investegate the effect of phosphorylated-P38MAPK(mitogen-activated protein kinase) on the expression of caspase-3 in the substania nigra（SN）of MPTP-induced mouse model of（PD）. Methords: Mice were randomly divided into MPTP model group, which were treated with MPTP(30 mg/kg, dissolved in saline, intraperitoneal injection), inhibitor group, which were treated with SB203580(10 mg/kg, dissolved in 5 mg/ml DMSO, intraperitoneal injection) 1 h before injection of MPTP. Once a day for 5 days; control group were treated with saline and DMSO as much as the model group received per day for 5 days. The behavioral were observed, immunohistochemistry and western blot for TH, caspase-3 and phosphorylation of P38MAPK were used to observe the change of positive cell number numbers and the expression level in the SN of midbrain. Results: Compared with the mice in control group, the model group showed typical symtoms of PD with decreased numbers of TH-positive neurons and the protein level of TH in SN of the midbrain by about 60% and 65% respectively（P<0.01）, the numbers of caspase-3 and phosphorylation of P38MAPK immunoreactive cells and their protein level in the SN of the midbrain increased markedly（P<0.01). After giving SB203580, the above changes were reduced obviously（P<0.01）. Conclusions: In the mouse model of subacute Parkinson's disease induced by MPTP, phosphorylated-P38MAPK regulated caspase-3 in the SN of midbrain, the specific P38MAPK inhibitor SB203580 is neuroprotective to the mouse model.

ASPS induces G2/M arrest of H446 cells by activation of ERK signal pathway

Jun-xia ZHAO; Yong-xin YAN; Yan-ling WANG; Shuo HAN; Yun-li YAN

2010, 30(1):  59-62. 

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Objective To investigate ASPS induced G2/M arrest in small cell lung cancer cell lines H446 and its effect on ERK MAP kinase signal transduction pathways. Methods Cell cycle phases were inspected by flow cytometery (FCM) ; Western blot analysis inspected the proteins of ERK, p-ERK; Results Compared with control group, G2/M phase cells increased with concentration increase significantly, G0/G1 phase cells were no difference, G2/M phase cells and G0/G1 phase cells were no difference when pre-incubated with PD98059 prior to exposure to ASPS of different concentrations, proteins of p-ERK were increased significantly, expression of ERK were no difference. Conclusion ASPS may induce G2/M arrest of H446 cells possibly by activation ERK MAP kinase pathways.

Changes of LHR, INSR, AR genes' methylation in rat polycystic ovarian syndrome model

Liang ZHU; Fu-qi XING; Song QUAN; Wen-ying ZHANG; Jian-xin DU

2010, 30(1):  63-66. 

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Objective To establish an ideal model animal of PCOS and to detect the DNA methylation states of LHR, INSR and AR genes in this model. Methods 24-days-old female Sprague-Dawley (SD) rats were randomly divided into two groups. The rats in the experimental group were given subcutaneous implanting of levonorgestrel silica gel staff (3mm per rat) and begun to inject 1.5 IU hCG twice daily for 9 days from the 4th day. The rats in the control group were injected with normal saline at the same time. Ovarian morphologic changes, sex hormone levels, fasting serum insulin and glucose were detected. The LHR, INSR and AR genes' DNA methylation patterns were checked by methylation specific PCR in modeling group and control group. Results The ovarian weight and volume in modeling group were higher than those in control group (both P< 0.001). The ovaries in modeling group showed multiple follicular cysts, and the number of theca cells and interstitial cells increased. Less developing follicles and corpus lutea were seem. The serum level of progesterone, testosterone, luteinizing hormone, fasting insulin and glucose were significantly higher in experimental group than those in control group (P<0.05), so as the LH/FSH ratio and HOMA index (both P< 0.001). No methylation of LHR and AR genes were detected in both groups. The methylation frequency of INSR gene (76.7%) was significantly higher in modeling group than that in control group (P< 0.001). Conclusions The depression of INSR gene's transcriptional induced by DNA methylation is a good supplement to he pathogenesis of insulin resistance in PCOS.

Role of stroma cell-derived factor-1 receptor in utero transplantation

Wen-chao OU; Dong-sheng CHI; De-sheng SUN; Shu-kun LU

2010, 30(1):  67-70. 

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Objective To explore the effect of stroma cell-derived factor receptor CXCR4 on the homing of the hematopoietic stem/progenitor cells in utero transplantation. Methods CD34+ cells were separated by Ficoll density gradient centrifugation and MiniMACS and stimulated for 48h by SCF、IL-6 cytokines prior to transplantation. The changes of the CD184(CXCR4) expressions and transmigrate rates of the CD34+ cells were analysed by flow cytometer. The 1 105 cells pretreated with different treatment were transplanted into the abdominal cavity of the fetal BALB/c mouse in the pregnant days 13~14. The human CD45 cells as the marker of graft were detected by flow cytometry after 1 month the fetus born. Results Expression changes of CD184 on CD34+ cells were from 9.58% 1.56％ to 19.32% 3.64％ after SCF and IL-6 stimulation. The CD34+/CXCR4high cells exhibited significant increases in SDF-1 mediated chemotaxis compared with the CD34+/CXCR4low cells. Transmigration of CD34+/CXCR4high was inhibited by pretreatment with antiCXCR4mAb and PTX. The positive rates of human CD45 cells detected in the fetal mouse were significantly higher in the SCF and IL-6 pretreatment group. This effects were significantly abrogated after the addition of antiCXCR4mAb or PTX. Conclusion Up-regulation of CXCR4 expression may be useful for improving hematopoietic stem/progenitor cells homing in utero transplantation. This homing process is mediated and depended on the CXCR4 receptors. The signal transduction is mediated by PTX-sensitive Gi protein.

Acute Application of ApoE4 increased the Neuronal Resting [Ca2+]i of the Rat Cortical

Xin-ai LIU; Xin-yi LI

2010, 30(1):  71-74. 

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Objective To investigate the acute effect of apolipoprotein E（apoE）on the intracellular free Ca2+ in rat cortical neurons. Methods The intracellular resting calcium level in cultured primary rat cortical neurons was measured by using confocal fluorescent imaging technique. MK-801, an N-methyl-D-aspartate (NMDA) receptor noncompetitive antagonist, was employed to test whether NMDA receptor was implicated in the effect of apoE4. Results Acute application of apoE4, but not apoE3, significantly increased the resting [Ca2+]i in a dose- and time-dependent manner (P<0.01 or P<0.05), and MK-801 partly blocked the apoE4-induced elevation of resting [Ca2+]i (P<0.05 or P<0.01). Conclusion ApoE4 acutely disturbs calcium homeostasis, and the activation of NMDA receptor may play a critical role in the intracellular calcium overload induced by apoE4 and its neurotoxicity.

Cloning, expression and bioactivity analysis of Human Granulysin

Wan-xia WANG; Xi LAN; Xiang-hong XU; Jun JU; Ji-xing LIU

2010, 30(1):  75-79. 

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Objective To obtain recombinant human granulysin using prokaryotic expression system. Methods Total RNA was extracted from cultured PBMC. Granulysin gene segments were obtained with granulysin-specific primers by RT-PCR and then inserted into pET32a(+) plasmid. After identification by DNA sequence, pET-GNLY9K and pET-GNLY15K were transferred to E. coli Rosetta (DE3).Fusion proteins were expressed under induction of IPTG.The fusion proteins was identificated by SDS-PAGE and Western-blot.The bioactivity of granulysin fusion protein was measured by MTT assay. Results Restriction enzyme digestion and sequence analysis showed that prokaryotic expression vectors pET-GNLY9K and pET-GNLY15K were successfully constructed.The corresponding molecular weight of 31 and 37kD fusion protein were highly expressed in E. coli after induction. Recombinant proteins could specifically bind to anti-granulysin antibody.MTT assay analysis showed that GNLY9K fusion protein could significantly inhibit the growth of A549 cells in a dose-dependent manner,while GNLY15K had little effect on the growth of A549.Conclusion Different molecular weight granulysin were successfully expressed using prokaryotic expression system,which would be helpful for the further study of granulysin.

临床园地

Analysis of 222 Cases of Acute Myeloid Leukemia Karyotype and Chemotherapeutic Effect

Bei LIU; Juan LI; Xuan CHEN; Yu JIN

2010, 30(1):  80-83. 

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Objective To explore the relationship of acute myeloid leukemia kayotype with chemotherapeutic effect. Methods Conventional cytogenetic technique of G-band was used to analyze in 222 acute myeloid leukemia patients. Results　 Eighty-six cases(42.1%) with cytogenetic abnormalities were found in 204 patients.Excepting for M3 patients ,the favorable,intermediate and adverse groups according for MRC criteria were treated with TA or CAG schema respectively.The CR rate of M3 was obviously higher than non-M3.The favorable and intermediate group were obviously surpassinger than adverse group in TA schema.The CR rate of the intermediate group was highter than that of the other groups in CAG schema.The CR rate of the adverse group in two schema were all low.The CAG was surpassinger than TA schema in intermediate group. Conclusions Cytogenetics is an important chemotherapeutic and prognostic factor to acute myeloid leukemia. CAG schema could be the leader selection in the de novo presby or oligoaccrementional AML patients.

The clinical characteristics of pneumomediastinum in patients with dermatomyositis and polymyositis

Jin-mei SU; Hua CHEN; Dong XU; Yong HOU; Xi-qin SUN; Wen ZHANG; Fu-lin TANG

2010, 30(1):  84-86. 

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Objective To analyze the clinical characteristics of pneumomediastinum in patients with dermatomyositis and polymyositis and to study its pathogenesis and prognosis. Methods The clinical records of 96 patients with PM/DM were reviewed, focusing mainly on the presence of pneumomediastinum. Five patients with pneumomediastinum are described in detail. Case reports of pneumomediastinum in PM/DM in English publications are reviewed. Results Five DM cases complicated by pneumomediastinum all had lung infections. 29 cases (including our five cases) of DM/PM with pneumomediastinum have taken methylprednisolone, four cases alive, and six died. Ten cases have taken CsA，eight cases alive and two died. Conclusions The infections was strongly suspected as being responsible for the pneumomediastinum. methylprednisolone has poor effect. CsA can be an effective therapeutic agent in PM/DM.

研究短文

Detection and Its Clinical Significance of HBV Full-Length cccDNA from Serum

Wei CHEN; Qun-ying LIU; Xiao-feng ZHANG; Chao DENG; Ju-ping BAO

2010, 30(1):  87-88. 

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Effects on Scorpion Venom Fibrinolytic Active Peptide of human vascular endothelial cells of hypoxic injury

Qiao-yun WANG; Feng-jie WU

2010, 30(1):  89-90. 

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Apoptosis induction of Aspirin on human cervical cancer cell lines HeLa

Jian-lin YANG; Yu HAN; Yong-qin ZHOU

2010, 30(1):  91-92. 

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短篇综述

Progress in Pharmacological Action of Gossypol

Zhong GUO; Jin ZHAO; Jian-xiu MA

2010, 30(1):  93-96. 

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Gossypol is a kind of naturally yellow polyphenolic compounds extracted from root，stem and seed of the cotton plant. It had received significant attention for its potential application as a male antifertility agent. Then it was used to treat female hormone-dependent diseases，for instance, endometriosis，hysteromyoma，uterine bleeding and dysmenorrheal. Further researches showed that gossypol has multiple biological activities，such as anti-inflammatory，antimalarial，antiviral antioxidant activities and so on，especially the ability to induce tumor cell apoptosis.

Progress on individual therapy of colorectal cancer research

Jin LI; Jing-dong HE

2010, 30(1):  97-99. 

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Chemotherapy plays an important role in advanced colorectal cancer.patient may acquire various clinical response by selection different drugs .the importance of individual therapy which based on Pharmacogenomics has been emphasized in cancer therapy. To explore the individual therapy of colorectal cancer ,the progress of molecular markers which influence the efficacy of anti-tumor drugs containing Fluorouracil,Oxaliplatin,CPT-11, Monoclonal antibody drugs is reviewed in this article.

Progress on Aberrant Methylation of INK4a/ARF and ASPP Genes in Tumor

Hong-ling LI; Chun-hua ZHAO

2010, 30(1):  100-102. 

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Many tumorigenesis in human are closely linked to the functional loss of P53. The tumor-suppressive functions of P53 are abrogated by mutations, but in 50% of all tumors remains wild type. In these cases, loss of P53 tumor-suppressive function mainly results from the abnormal methylation of 5' CpG islands of tumor suppressor genes such as INK4a, ARF and ASPP. Restoring to normal methylation state of these genes may provide new means for tumor therapeutics.

The relation between cardiac development and wnt /β-catenin signal transduction in early embryo

Jun CHEN; Li SUN

2010, 30(1):  103-106. 

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The wnt/β-catenin signal transduction pathway is an access regulating cell proliferation, movement, differentiation and plays crucial roles in embryo development. The researchers have found that Wnt pathway is an important regulation factor in the early stimulation of the cardiogenesis and contacts closely the several key events during cardiac morphogenesis. The article reviews the recent studies about the relation between cardiac development and Wnt pathway in early embryo.