Abstract: Objective:To explore the effect of gene NDRG2（N-myc downstream regulated gene 2） transfection on the proliferation and apoptosis in breast cancer cell. Methods : Bcap37 cell line express low level NDRG2 gene is transfected with recombinating adenovirus expressing high level NDRG2 gene to improve NDRG2 gene expression level. The NDRG2 expression level was detected by Western blot and the proliferation activity of the cell lines was detected by MTT and flow cytometry. Results : High expression level of NDRG2 inhibits cells growth and results in G1 phase arrest in Bcap37 cell line. Compared with non-transfected Bcap37 cells and Bcap37 cells transfected by empty vector, apoptosis cells obviously increase in Bcap37 cell line transfected by recombinating adenovirus expressing high level NDRG2 gene, 12% after 48 hours and 21.5% after 72 hours. Conclusions: Overexpression of NDRG2 in Bcap37 cells effectively inhibited cell proliferation and induced cell cycle arrest and apoptosis.

Key words: NDRG2, tumor, gene transfection, adenovirus