Table of Content

    05 December 2010, Volume 30 Issue 12
    In vivo and in vitro research on inhibiting effects of DNAzyme on EBV-LMP1 of NPC cells
    KE Xia ;YANG Yu-cheng ;HONG Su-ling
    2010, 30(12):  1245-1251. 
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    Objective To explore the inhibitting effect of 1 0-23DNAzyme on the growth of nasopharyngeal carcinoma (NPC) cell,EBV-LMP1both in vivo and in vitro.Methods Thosthorothioate 1O- 23DNAzyme and analogs targetted on EBV-LMP1 (Epstein-Barr virus encoded latent membrane protein 1)gene mRNA were designed and synthesized ,and transfected into C666-1 cells with lipofectamine ,observing the inhibiting effect on LMP1 . Xenografts of NPC in nude mice were formed ,and after DNAzyme and analogs were injected in tumor, the depressive effects on the growth of tumor and LMP1 gene were assessed.Results The results showed that DNAzyme inhibited the expression of LMP1 gene in vivo and in vitro ,and also the growth and metastasis of xenograft of NPC were depressed,and it was found that the effect of DNAzyme, was better than that of its analogs. (P〈0.05). Conclusion 10-23DRz can strongly block the expression of EBV-LMP1gene in the C666-1 cell model and xenograft in nude mice .It might serve as a specific and effective anti-LMP1 gene therapeutic candidate agent.
    Baicalin Inhibits VSMC Proliferation and Neointimal Hyperplasia in Rats
    HU Hai-juan ;HAN Mei ;SUN Rong-hua ;LIU Bin ;WEN Jin-kun
    2010, 30(12):  1252-1256. 
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    Objective The present study was designed to elucidate the effect of baicalin on the proliferation of vascular smooth muscle cells (VSMCs) and the neointimal hyperplasia after balloon injury and the related mechanism. METHODS Cell culture, MTT assay, Western blotting and immunohistochemical staining were adopted to evaluate the inhibitory effects of baicalin on VSMC proliferation. RESULTS Baicalin inhibited cell proliferation induced by PDGF in a dose-dependent (5、10、20、40 μmol/L) manner, reduced proliferating cell nuclear antigen (PCNA) expression and blocked PDGFR-MEK-ERK1/2 signaling pathway activated by PDGF in VSMCs. In vivo, baicalin significantly inhibited neointimal hyperplasia induced by balloon injury. The ratio of intima-to-media area (I/M) in baicalin group was significantly less than that in balloon injury group (P<0.01). The expression of PCNA, ICAM-1 and VCAM-1 were significantly reduced in baicalin group, compared with the balloon injury group. CONCLUSIONS Baicalin inhibits neointimal hyperplasia via inhibiting VSMC proliferation and adhesion molecule expression induced by balloon injury.
    The role of Nrf2 in the expression of UGT1A and its isoforms induced by EGCG in colon tumor
    YUAN Jun-hua ;LI Yan-qing ;YANG Xiao-yun
    2010, 30(12):  1257-1262. 
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    Objective To investigate the role of nuclear factor Nrf2 in the expression of UGT1A and its isoforms induced by EGCG in colon cancer. Methods We used the technology of RNA interference to block the expression of Nrf2 in HT-29 cell line in vitro, and cultivated the cell line to the phase of logarithmic growth. Using HT-29-siNrf2 cell line we constructed subcutaneous cancer model in the BALB/cA nude mice. Using subcutaneous tumor we constructed orthotopic colon cancer model in nude mice. After having been administrated with low-, medium- and high-dose EGCG for 4 weeks,the orthotopic colon cancer was peeled. The protein expression of Nrf2 of colon cancer was detected by IHC and the mRNA levels of Nrf2, UGT1A, UGT1A8 and UGT1A10 were detected by RT-PCR. Results The body weights of the mice,the inhibition rate of orthotopic colon cancer and the protein expression of Nrf2 detected by IHC had no significant difference compared with those of mice in model group. The mRNA levels of Nrf2, UGT1A, UGT1A8 and UGT1A10 detected by RT-PCR had no significant difference. Conclusion Nrf2 could play a critical role in the expression of UGT1A and its isforms induced by EGCG.
    Differentiation of human induced pluripotent stem cells into neural stem cells
    FENG Nian-hua;XIE An ;LOU Yuan-lei ;RUAN Qiong-fang ;GUO Fei ;WANG Yang
    2010, 30(12):  1263-1267. 
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    Objective To observe the differentiation potential of induced pluripotent stem (iPS) cells to neural stem cells (NSCs). Methods The differentiation process was carried out through retinoic acid (RA) inducing procedure. The transformation of cell morphology was observed using inverted microscope. RT-PCR was used to measure the expression of pluripotent genes. Specific markers of neural linage cells such as NESTIN, SOX2, β-TUBULINШ, GFAP were also detected by immunostainning. Results Neural tube-like constructions appeared 4 days after retinoic acid (RA) induction, these cells expressed NESTIN and could differentiate to β-TUBULINШ positive neurons and few GFAP positive astrocytes. There were no neural tube-like constructions in control group. Conclusion Human iPS cells can differentiate toward neural stem cells and can imitate the development process of nervous system.
    The enhancement of chemosensitivity to cisplatin of human bone marrow mesenchymal stem cells by tumor suppressor protein p73
    LIANG Wei ;LU Chun-hua ;LI Jing ;ZHAO Chun-hua ;ZHANG Jing-bo
    2010, 30(12):  1268-1373. 
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    Objective To investigate the role of P73α in relation to cellular chemosensitivity in cultured human bone marrow mesenchymal stem cells. Methods To construct the retrovirus vector expressing P73α and transfect human bone marrow mesenchymal stem cells.Chemosensitivity and apoptosis were examined in the exogenous over-expressed cells. Results Our results showed that retrovirus can successfully induce human P73α expression in bone marrow mesenchymal stem cells, over-expression of ectopic P73α resulted in a significant increase of cellular sensitivity to cisplatin. Enhanced chemosentivity in P73α transfected cells was attributed by the increase of cellular apoptosis. Moreover, co-activation of pro-apoptotic factors Bax and P21 was observed in the transfected cells after cisplatin treatment. Conclusion Our findings suggested that P73α is an important determinant of cellular chemosensitivity to cisplatin in the human bone marrow mesenchymal stem cells.
    Mutation Analysis of HRAS gene in a Chinese girl with Costello Syndrome and literature review
    ZHANG Han-bing ;QIU Zheng-qing ;WANG Wei ;ZHAO Shi-min ;QIU Jia-jing ;WEI Min
    2010, 30(12):  1274-1277. 
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    Objective To introduce the rare autosomal dominant Costello Syndrome through literature review after diagnosing the 1st Chinese patient by direct sequencing of HRAS gene. Methods Total genomic DNA was extracted from peripheral blood leukocytes of the patient and her parents. The coding exons 2-5 of the HRAS gene were amplified by polymerase chain reaction (PCR) and sequenced directly. Results A common HRAS missense mutation, p.Gly12Ser, was identified in the patient,but not in the parents. Conclusion Patients with Costello syndrome manifest characteristic facial features, feeding difficulties and developmental delay/mental retardation etc. The diagnosis can be confirmed by mutation analysis of HRAS gene.
    SOCS-1 reduced the cytokine-induced pro-apoptotic gene expression in pancreatic β cells
    SUN Qi ;XIANG Ruo-lan ;YANG Yan-li ;FENG Kai ;ZHANG Wen-long ;YANG Guo-hua ;SONG Ai-ling
    2010, 30(12):  1278-1283. 
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    Objective This paper aims to generate a cell line overexpressing SOCS1 protein and investigate the protective effect of SOCS-1 on apoptosis of pancreatic β cell. Methods The rat SOCS-1 coding sequence was amplified and subcloned into pEGFP-C1 vector. The recombinant plasmid pEGFP-C1-SOCS1 was transfected into RINm5F cell line (insulin-secreting tumor cell line). We generated a rat RINm5F cell line overexpressing recombinant plasmid pEGFP-C1-SOCS1. Cells were treated by several cytokines, including TNF-α, IL-1β, IFN-r, IL-1β+IFN-r, IL-1β+TNF-α+IFN-r. The cellular apoptosis were measured by RT-PCR and Western blot. Results We have generated a rat RINm5F cell line overexpressing SOCS1 protein successfully. The expression level of the iNOS mRNA was significantly increased in RINm5F cells after treated by IFN-r, IL-1β+IFN-r, IL-1β+TNF-α+IFN-r, but not SOCS1-RINm5F cells. Western blot analysis demonstrated that the expression and activity of caspase-3 was significantly increased in RINm5F cells after treated by all cytokines. However, the caspase-3 expression and activity was not significantly different in SOCS1-RINm5F cells after treated by cytokines. Conlusion Overexpression of SOCS-1 can reduced cytokine-induced pro-apoptotic gene expression in pancreatic β cells.
    Bioinformatic analysis of a novel gene GP1 differentially expressed in gastric cancer and its premalignant lesions
    ZHENG Jie ;WANG Li-na ;YU En-yan ;LV Shi-jun
    2010, 30(12):  1284-1287. 
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    Objective To analyze the gene and protein sequence of a novel gene GP1 with bioinformatics and explore its action in process of the gastric carcinogenesis and development. Methods Based on the human genome resource, Megablast and SAGE were used for the analysis of chromosome location and tissue expression of GP1; BLAST procedure was used to analyze the gene sequence and the similarity gene; ORF finder, ProParam, ProtScale and so on have been performed on GP1, sequence of the GP1 encoding protein, the physical and chemical characteristics, subcellular localization, functional domain and so on are predicted. Results The full length of the GP1 is 1362bp, the longest ORF is 801bp and encoded 267 amino with a low homologous eIF5C domain.GP1 gene is expressed in different tissues. Conclusion Bioinformatic analysis of GP1 showed that GP1 is a gastric cancer associated gene and may be involved in the process of the gastric carcinogenesis and development.
    Adiponectin gene SNP-11377 and SNP-4522 haplotypes are associated with metabolic syndrome
    LI Yi-ping ;YANG Man ;XIONG Yu-xin ;LI Xian-li ;XU Bo ;YAO Yu-feng ;ZHANG Ying
    2010, 30(12):  1288-1292. 
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    Objective To investigate whether the adiponectin gene single nucleotide polymorphisms (SNP) are associated with the metabolic syndrome. Methods Using polymerase chain reaction-based restriction fragment-lenngth polymorphism (PCR-RFLP) genotyped SNP-11391、SNP-11377、SNP-4522、SNP+45 and SNP+331 of adiponectin gene, and estimated the association of the five SNPs with metabolic syndrome(MS). Results ⑴Adiponectin gene SNP-11391 and SNP+331 are not polymorphisms in Han population of Kunming City. ⑵In MS group, SNP-11377G-SNP-4522T haplotype frequency is lower than control group (P﹤0.05), SNP-11377C-SNP-4522T and SNP-11377G-SNP-4522C haplotype frequencies are higher than control group (P﹤0.05 and P﹤0.01) ; Conclusions In Han population of Kunming City, SNP-11377G-SNP-4522T haplotype maybe associated with lower risk of MS, and SNP-11377C-SNP-4522T and SNP-11377G-SNP-4522C haplotypes maybe associated with higher risk of MS.
    Simvastatin decreased the osteopontin expression in lipid-induced rats renal injury
    CHEN Li-qun ;GAN Hua ;LV Zhi-mei
    2010, 30(12):  1293-1298. 
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    Objective To investigate the expression of osteopontin (OPN) and monocyte chemoattractant protein 1(MCP-1)and the effect of simvastatin on them in diet-hypercholesterolemia rats. Methods Wistar rats were divided into three groups: control rats, cholesterol fed rats and cholesterol fed rats treated with simvastatin (10 mg ? kg-1? d -1). Twelve weeks later, we measured the 24 hours total urine protein, creatinine clearance , total serum cholesterol, LDL cholesterol.HDL cholesterol and triglycerides. kidney pathology was observed. Immunohistochemistry was used to analyse the expression of MCP -1. The expression of OPN in kidney tissue were detected by RT-PCR and western blot. Results As compared to control group rats ,the total serum cholesterol, LDL—cholesterol level, triglycerides and 24 h total urine protein increased in hypercholesterolemia group rats(P<0.01) . but it decreased significantly in simvastatin treated group compared with hypercholesterolemia group(P<0.01). the renal pathological lesion was ameliorated in simvastatin treated group; As compared to control group rats ,the expression of MCP-1 increased in cortical tubular epithelium and the expression of OPN increased in hyperlipemia group, and the expression of MCP-1 and OPN was significantly decreased in the simvastatin treated groups after 12 weeks(P<0.05). Either the expression of MCP-1 or urine protein was positively correlated with the expression of OPN in renal tissues.Conclusion The expression of OPN and MCP-1 were increased and had a close relationship with macrophage infiltration into tubulointerstitium in Hypercholesterolemia rats.Simvastatin has some protective effect partly through down regulating OPN and MCP-1 expression and reducing macrophage infiltration.
    Somatic mutations in the mitochondrial DNA control region and breast tumor pathogenesis in Yunnan Province
    YANG Yue ;HE Jun-dong ;GUO Xue-jun ;CHE Yan-hua ;ZHANG Yong ;KONG Qing-peng ;ZHANG Ya-ping ;LI Li
    2010, 30(12):  1299-1302. 
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    Objective To investigate the somatic mutations in control region of MITOCHONDRIAL DNA (mtDNA) in patients with breast tumor in Yunnan Province. Methods The DNA of tumor tissue and peripheral blood in 28 breast cancer patients and 13 benign breast tumor patients were extracted and mtDNA D-loop HVR1 and HVR2 fragments were amplified with designed two pairs of primers by PCR. The mutations of products were screened by sequencing and compared the mutations between tumor tissue and peripheral blood. Results 8 somatic mutations in 28 breast cancer patients were found, including 309+CC (sample BC6), 309+C (sample BC20, sample BC26), 279Y (sample BC16), 297R (sample BC16), 214R (sample BC21), 190Y (sample BC61) and 204Y (sample BC64); 2 somatic mutations in 13 benign breast tumor patients were found, including 16292Y (sample BCA10) and 309+CC (sample BCA12). Conclusion Our data suggest that somatic mutations in mtDNA D-loop HVR1 and HVR2 may play a role in the pathogenesis of breast cancer in Yunnan Province.
    Preparation and characterization of recombinant poly-epitope peptides based on SARS-CoV B cell recognized linear epitopes
    ZHOU Jian-hua ;ZENG Yu-hong ;CUI Lian-xian ;KANG Ning ;HE Wei
    2010, 30(12):  1303-1308. 
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    Objective To obtain recombinant poly-epitope peptides based on selected linear B cell epitope and evaluate its probability as epitope vaccines. Methods Poly-epitope peptides were expressed by prokaryotic expression vector with multi-epitope peptides coding gene fragments, which were obtained through gene engineering technology. The cross-reactivity of tested peptides with the SARS patients sera and titers to serum from rabbits immunized with recombinant poly-epitope peptides were determined by ELISA. Their specificity was measured by Western blotting, while SARS-CoV-neutralizing response was identified by neutralization technology. Results PEP1 and PEP2 encoding gene fragments were successfully obtained and the recombinant poly-epitope peptides rPEP1 and rPEP2 were successfully expressed. Cross-reaction rate of purified rPEP1 and rPEP2 with the patient serum were 88.7% and 97.2%, respectively. They can be recognized by immunized rabbits serum, binding titers were 1×106 and 5×105, and SARS-CoV neutralizing efficiency were 70% and 80% respectively. Conclusions Recombinant poly-epitope peptides rPEP1 and rPEP2 with high cross-reactivity, strong immunogenicity were successfully obtained. It provided experimental basis for the preparation of poly-epitope peptide vaccine.
    Hippocampal neuronal apoptosis and the increase expression of caspase-8 and AIF after chronic alcoholism in rats
    DUAN Yu-xiang ;SHI Qiu-yan ;JIANG Jin-ke
    2010, 30(12):  1309-1312. 
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    Objective To investigate hippocampal neuronal apoptosis and the expression of caspase-8 and AIF protein in the model rats after chronic alcoholism. Methods Forty-five male SD rats,which were eight weeks old, were divided into two groups randomly: Control group(n=20)and alcohol group(n=25). The experiment adopted free drinking by increasing alcohol percentage gradually . Memory function of rats was detected by Morris water maze . Pathological changes were observed by HE staining, apoptosis cells and the expression of caspase-8 and AIF protein of each group were quantitatively examined by TUNEL and Immunohistochemical method. Results In alcohol group,there are decrease and denaturalization of nerve cells in hippocampus. At the same time,the numbers of the neural apoptosis and the expression of caspase-8 and AIF protein in the alcohol group are higher than that of the control group(p<0.01).Conclusion There are obviously neural apoptosis as well as the expression of caspase-8 and AIF protein in the rats hippocampus after chronic alcoholism. One of mechanisms of cerebral trauma in chronic alcoholism maybe through both caspase-dependent and caspase-independent pathways.
    Intravenous administration of Human Umbilical Cord Blood Cells (HUCBC) enhances angiogenesis in rabbits with acute myocardial infarction
    PAN Wei ;ZHOU Bo ;WANG Tao ;QING Li-qiong ;ZHANG Guo-gang ;YANG Tian-lun ;YU Guo-long
    2010, 30(12):  1313-1317. 
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    Objective To investigate the effects of intravenous administration of Human Umbilical Cord Blood Cells (HUCBC) on the angiogenesis in rabbits with acute myocardial infarction. Methods Rabbits were randomly divided into sham operation group, model and cell transplantation groups, with 15 animals in each group. Rabbits were performed anterior descending branch braid without ligation in sham group; Rabbits acute myocardial infarction models were prepared in cells transplantation group and model group. At hour 24 after model preparation, 0.5 mL HUCBCs suspension (containing 2×107 cells) was intravenously administrated into rabbits in the cell transplantation group. The same volume of saline was administrated into the other two groups. Cardiac functions were performed by echocardiography at 1st week, 2nd week and 4th week after transplantation. The survival of transplanted cells and microvessle density in the myocardium were identified by immunohistochemistry. The expression of VEGF mRNA in the myocardium was examined by RT-PCR. Results Transplantation of HUCBC improved left ventricular function indexes such as LVFS, LVEF at all 3 examinations(P<0.05); Immunohistochemistry showed that BrdU positive cells were found only in the transplantated rabbits. Compared with control group, there was a significant increase of microvessle density within the boundary of infarcted myocardium in cell transplantation rabbits(P<0.01). Compared with control group, there was an markedly higher level of the expression of VEGF mRNA in the transplantated group(P<0.01). Conclusions Angiogenesis induced by the HUCBCs might be the one of main mechanisms for stem cell transplantation for myocardial infarction.
    Binding of avian influenza A (H5N1) to gangliosides on the surface of A549 cells and Hep-2 cells
    HUANG Li ;GUO Xian-rong ;TU Yan-hui ;SONG De-lu ;YANG Hao ;YIN Hong-chao
    2010, 30(12):  1318-1324. 
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    Objective To study the attachment and subsequent entry of H5N1 virus to A549 cells and Hep-2 cells. Methods Expression of SAa2,3Gal and SAa2,6Gal on A549 cells and Hep-2 cells was examined by using lectin fluorescence and flow cytometry. H5N1 virus entry into A549 and Hep-2 cells was measured by indirect immuno?uorescence assay. The entry efficiency was determined by Western blot analysis. Results SAa2,3Gal was prevalent in A549 cells and Hep-2 cells, while SAa2,6 Gal was little found. Furthermore, SAa2,3Gal expression was more regularly observed in Hep-2 cells, rather than A549 cells. The H5N1 virus tested could enter A549 cells and Hep-2 cells. However, viral entry efficiency differed between the two cell lines tested. A549 cells were found to be more susceptible to avian influenza than Hep-2 cells. H5N1-induced cell death was inefficient in A549 cells than Hep-2 cells. Conclusion The expression of SAa2,3Gal on the cells tested corresponded with the attachment of the H5N1 virus. However, sialic acid only may not sufficient for entry into cells.
    Clinical evaluation of prostate cancer spatial distribution
    YAN Wei-gang ;LI Han-zhong ;ZHOU Yi ;CUI Quan-cai
    2010, 30(12):  1325-1328. 
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    Objective We analyzed the prostate biopsy data obtained with a transperineal ultrasound and thereafter navigated the 11-region prostate biopsy template in a sample population to evaluate prostate cancer spatial distribution characteristics. Methods From May 2004 to December 2007, 215 patients with a median prostate-specific antigen (PSA) level of 21.0 ng/ml (range 1.2 to 100) were diagnosed with prostate cancer through the transperineal ultrasound guided 11-region template prostate biopsy at Peking Union Medical College Hospital. The characteristics of our sample cancer spatial distribution were assessed in relation with different PSA levels. Results The mean positives for the cancer of regions 1-10 and region 11 (the apical region) were 61.2% vs. 66.4% in patients whose PSA > 20ng/ml , and 35.7% vs. 47.6% in patients with PSA ≤ 20ng/ml (P<0.001). The positives for cancer contained within the anterior and posterior areas were 96.5% vs. 90.9% in patients with PSA > 20ng/ml and 75.2% vs. 75.2% in patients with PSA ≤ 20ng/ml. Conclusions Our findings suggest that there is no difference in cancer distribution between anterior and posterior parts of prostate. Prostate cancers in PSA level less than 20ng/ml patients are more frequently found in the apical region, but no different rate is found in these regions in higher PSA level patients.
    Correlation of Imatinib Plasma Concentration and Efficacy in GIST Patients
    SUN Nai-pin ;LI Jian ;GAO Jing ;SHEN Lin
    2010, 30(12):  1329-1333. 
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    Objective To detect the plasma concentration of Imatinib in Chinese gastrointestinal stromal tumor (GIST) patients, and analyze the relationship between Imatinib plasma concentration and efficacy. Methods We collected 117 plasma samples from 112 GIST patients treated with Imatinib over 28 days. Plasma concentrations of Imatinib were detected using high performance liquid chromatography-mass spectrometry (HPLC-MS/MS). The correlation between Imatinib plasma concentration and efficacy was statistically analyzed. Results The plasma concentrations of Imatinib in high dose (600 mg/day) patients were significantly higher than that in low dose (400 mg/day) patients (P<0.001). Plasma concentrations in patients treated with Imatinib 1-2 years were higher than that in patients treated less than 1 year (P=0.028). The efficacy was increased along with the increase of plasma concentration of Imatinib, although there was no significant difference between them (P=0.546): the efficacy in low plasma concentration, middle plasma concentration and high plasma concentration was 50%, 67.5% and 66.7%, respectively. Conclusion There were significant correlations between Imatinib plasma concentration and drug dose or time of drug administration; the efficacy of Imatinib was increased along with the increase of plasma concentration.
    Analysis of alternaria alternate allergen
    CAO Nai-qing ;ZHANG Hong-yu
    2010, 30(12):  1334-1335. 
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    Nitric oxide can relieve lung injury after ischemia-reperfusion of hind limbs
    KONG Xiao-yan ;MEN Xiu-li ;DONG Shu-yun ;LI-Hong-jie ;ZHAO Li-jun ;DUAN Guo-xian ;ZHANG Lian-yuan
    2010, 30(12):  1336-1337. 
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    Analysis and Reflection Of Current Laboratory Diagnosis on Invasive Candidiasis
    HU Dong-mei ;CHE Xiao-yan
    2010, 30(12):  1338-1341. 
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    Ideal diagnostic methods of invasive candidiasis, the fourth nosocomial infection are urgently desirable to date. Even though great progress has been made on the subject which uses the antigen, antibody and nucleotide as the detection targets, they are hard to solve the practical problems confronted to clinical requirement due to issues such as sensitivity, specificity, standardization and design of study et al. Highly conserved and rich diagnostic markers might be a good solution to such problem.
    Wnt/β-catenin Signaling Pathway and Kidney Diseases
    YAN Zhe ;DUAN Hui-jun
    2010, 30(12):  1342-1345. 
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    Wnt/β-catenin signaling pathway is involved in kidney development and many kidney diseases, including kidney cancers, renal fibrosis, Polycystic kidney disease, acute renal failure and diabetic nephropathy. The pathway control ureteric bud development and regulate nephrogenesis in the mesenchcymal cells ;It also plays a critical role in the oncogenesis and progression of many kidney cancers; Aberrantly activation of Wnt/β-catenin signal induces proliferation or apoptosis of glomerular mesangial cells and renal tubular epithelial cells , which leads to pathogenesis of many kidney diseases. These important roles of the Wnt/β-catenin signaling pathway in kidney development and diseases are considerably consered.
    Research progress of relationship between miRNAs and hepatocellular carcinoma
    LI Yan-chun ;WEN Ji-fang
    2010, 30(12):  1346-1348. 
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    Recently, a new group of molecules, microRNAs, has been discovered to be aberrantly expressed in HCC and some of them are functionally involved in HCC carcinogenesis and progression. Certain microRNAs are associated with HCC subtypes, implying that microRNAs have a potential role in the diagnosis and prognosis of HCC; some of these HCC-associated microRNAs have been validated in independent cohorts, providing a molecular marker for early screening for clinical and risk assessment, but also providing a molecular basis for individual treatment of HCC.
    Effect of perturbating F-actin cytoskelecton on NF-κB activation
    GUO Wei-kang ;LIU Wen-hu ;ZHANG Dong-liang
    2010, 30(12):  1349-1351. 
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    The activation of the NF-κappa B signal transduction and the construction of the actin- cytoskeleton are simply introduced. The mechanism that NF-κappa B can be influenced through perturbation of the F-actin cytoskeleton is emphasized and prospect of this research is also introduced.
    Intermittent fever for five months
    2010, 30(12):  1352-1356. 
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